Download Updated - PeproTech Posters

MAY 2011
TIGAR-TAT (Catalog #150-14T)
Synonym: TP53-induced glycolysis and apoptosis regulator
Description: TIGAR is a p53-inducible enzyme that catalyzes the hydrolysis of fructose-2-6-bisphosphate (F-2-6-BP)
to fructose-6-phosphate and inorganic phosphate. F-2-6-BP is
a powerful activator of 6-phosphofructose-1 kinase, the rate
limiting enzyme of glycolysis. By lowering the intracellular
level of F-2-6-BP, TIGAR expression leads to increased glucose processing via the pentose phosphate pathway, the major cellular source for NADPH. NADPH plays a key role in
maintaining the cellular redox state by regenerating reduced
glutathione, which is critical for cellular protection against
mitochondrial-derived reactive oxygen species (ROS). Consequently, TIGAR expression modulates p53-induced apoptosis in response to ROS-associated DNA damage. Since elevated levels of F-2-6-BP are required for cell growth and
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Pretreatment with recombinant human TIGAR-TAT for 4 hrs, using a concentration range 0.5 - 1.0 mg/ml, has been shown to protect U2OS cells from apoptosis
induced by hydrogen peroxide. This activity demonstrates that recombinant human TIGAR-TAT can successfully enter the cell when added to exogenous media
and, once in the cell, exerts the expected TIGAR biological activity.
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TIGAR-TAT
and vascular endothelium. It has been widely used as a molecular
marker for the identification, isolation, and manipulation of
hemopoietic stem cells and progenitors. CD34 can function as a
regulator of hemopoietic cell adhesion by mediating the attachment
of stem cells to bone marrow stromal cells or other bone marrow
components The full length human CD34 is a 385 amino acid
protein, consisting of a 31 amino acid signal sequence, a 74
amino acid cytoplasmic domain, a 21 amino acid transmembrane
domain and a 259 amino acid extracellular domain. Recombinant
human sCD34 is a 259 amino acid polypeptide containing only
the extracellular domain of the full length CD34 protein.
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proliferation, p53-induced TIGAR expression prevents outgrowth of cells harboring damaged DNA.
Protein transduction using TAT fusion proteins represents
an alternative methodology for introducing transcription
factors and other intracellular proteins into primary as well
as transformed cells. Recombinant human TIGAR-TAT
expressed in E.coli is a 36 kDa protein containing 284
amino-acid residues, including the 271 residues of full-length
TIGAR fused to a 13-residue C-terminal peptide containing
the TAT transduction domain (GGYGRKKRRQRRR).
GDF-7 (Catalog #120-37)
Synonyms: Growth/Differentiation Factor-7, BMP-12
Description: GDF-7 belongs to the TGF-β superfamily of growth
MANF (Catalog #450-06)
and differentiation factors. It is expressed selectively by roof
plate cells that are located in the developing embryonic central
nervous system, and has been shown to influence the neuronal
identity of cells within the central nervous system. GDF-7 has
also been implicated in the formation, maintenance, and repair
of certain cartilage and ligament tissue. Recombinant human
GDF-7 is a 28 kDa disulfide linked homodimer containing two
129 amino acid residues
Synonyms: ARMET, Arginine-rich protein (ARP)
Description: MANF (Mesencephalic Astrocyte-derived
Neurotrophic Factor) is a secreted neurotrophic factor that is
expressed in brain, neuronal and certain non-neuronal tissues.
It has been shown to promote survival, growth and function of
dopamine specific neurons. MANF and its structural homolog
CDNF, each contain an N-terminal saposin-like lipid binding
domain, and a carboxyl-terminal domain, which is not homologous
to previously characterized protein structures. MANF and CDNF
can prevent 6-OHDA induced degeneration of dopaminergic
neurons by triggering survival pathways in a rat experimental
model of Parkinson disease. Recombinant human MANF is
an 18.1 kDa protein consisting of 158 amino acids including 8
cysteine residues.
Fetuin A/AHSG (Catalog #140-13)
Synonyms: Alpha-2-HS-glycoprotein, Alpha-2-Z-globulin,
Ba-alpha-2-glycoprotien
Description: Fetuin A/AHSG is a human plasma glycoprotein
belonging to the Cystatin family of proteases inhibitors. It is
highly expressed in embryonic cells and adult hepatocytes, and
is expressed to a lesser extent in monocytes/macrophages. Fetuin
A/AHSG is a major serum protein component that exerts various
calcium dependent physiological activities, and can mediate
growth signaling in certain tumor cells. It also can act as a natural
antagonist against specific TGF-β and BMP signaling proteins,
Recombinant human HEK293 cell derived Fetuin-A/AHSG is a
heterodimeric protein containing a 280 amino acid A chain and
a 25 amino acid B chain. Due to glycosylation, recombinant
Fetuin-A/AHSG migrates at an apparent molecular weight of 45
– 55 kDa by SDS-PAGE run under non-reducing conditions.
sCD30 Ligand (Catalog #450-42)
Synonyms: TNFSF8, CD153 , CD30L
Description: CD30 ligand (CD30L) is a type-II membrane-
associated glycoprotein protein belonging to the TNF superfamily
and is expressed primarily on certain B cells, T cells, and
monocytes. CD30L binds specifically to CD30 (receptor),
which is expressed on activated, but not resting, B and T cells, in
lymphomas and various chronically inflamed tissues. CD30L/
CD30 interactions initiate a signaling cascade that can ultimately
lead to the activation of NF-KappaB. CD30L/CD30 signaling
exerts pleiotropic effects on normal cells, including cell death,
differentiation, or cell division. Certain diseases, including
Hodgkins’s lymphoma, allergic inflammation, diabetes (in NOD
mice), and mycobacterial infection can also be affected by CD30L/
CD30 signaling. The CD30L gene encodes for a 234 amino acid
type II transmembrane protein, which contains a 37 amino acid
cytoplasmic sequence, a 25 amino acid transmembrane domain
and a 172 amino acid extracellular domain. Recombinant human
soluble CD30L (sCD30L) is a 188 amino acid polypeptide
corresponding to the extracellular domain, and contains an 8
residue N-terminal His -Tag.
VAP-1 (Catalog #150-16)
Synonyms: Vascular Adhesion Protein-1, SSAO, HPAO,
Copper amine oxidase, Membrane primary amine oxidase
Description: VAP-1 is a type II membrane cell adhesion protein
sCD34 (Catalog #310-31)
Synonyms: Hematopoietic progenitor cell antigen CD34
Description: CD34 is a highly glycosylated type I membrane
protein that is selectively expressed on hematopoietic stem cells
-2-
belonging to the copper/topaquinone oxidase family. It is
primarily expressed on the high endothelial venules of peripheral
lymph nodes and on hepatic endothelia. VAP-1 can catalyze the
oxidative deamination of low molecular weight amines, and plays
an important role in the migration of lymphocytes to inflamed
tissue. Inhibition of VAP-1 can protect against inflammation
related damage to certain injured tissues. Additionally, VAP-1 can
function as a significant prognostic marker for certain cancers and
cardiovascular diseases. Recombinant human VAP-1 is a mixture
of monomeric and disulfide linked homodimeric forms of a 737
amino acid polypeptide corresponding to amino acids 27 to 763
of the VAP-1 precursor.
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