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BIO 311 C Introductory Biology I K. Sathasivan REVIEW LIST AND LEARNING OUTCOMES FOR EXAM 2 ORIGIN OF LIFE Cell and Molecular Biology - Book Chap. 4 After learning this topic, you should be able to 1. Recognize and rationalize the major time lines in the origin and evolution of life on earth. 2. Understand Miller and Urey’s experiment and basic results and make logical connections to the possible origin of life on earth. 3. Predict the logical process by which simple monomers were formed and led to polymers, protobionts and the early forms of cells. 4. Explain how the first possible macromolecule could be RNA to store genetic information and be able to catalyze reactions. CELL STRUCTURE AND FUNCTION Cell and Molecular Biology - Book Chap. 5 After learning this topic, you should be able to 1. Apply different methods of microscopy, centrifugation and gel electrophoresis commonly to study the structure and function of cells depending on the purpose of the experiment. 2. Recognize obvious images derived from various microscopes and reorder the sequence of events in a cell fractionation experiment. 3. Compare and contrast prokaryotic and eukaryotic cells; and plant and animal cells. 4. Analyze given information in Table or Figure formats and use them to answer given questions on the properties of various cell types. 5. Classify all the different components of a eukaryotic cell with a concept map. 6. Identify the eukaryotic cell components from a given diagram and assign their functions. 7. Apply the cell structure and function knowledge to selected examples of diseases, mentioned in class or discussions, with reference to some organelles or cell components. 8. Draw the structure of plant or animal cell and label the contents and write the function for each cellular component. 9. Connect the various cell parts in terms of their functions or flow of information or membranes. 10. Relate the functions of cytoskeleton elements in terms of the cell movements and transport. 11. Develop practical application of the knowledge about cell wall components in various organisms. 12. Design a cell with specific properties to perform specific functions to survive and reproduce. 13. Trace the pathway of proteins as they are synthesized, modified, sorted out and shipped to various organelles. 14. Interpret given numbers, graphs or figures from research papers on cell structure and function. BIOLOGICAL MEMBRANES Cell and Molecular Biology - Book Chap. 6 After learning this topic, you should be able to 1. Given the components of a biological membrane, draw a picture of fluid mosaic model of a membrane, name its components and list their functions. 2. Understand and apply how is the fluidity of the membranes is affected by its composition of saturated or unsaturated lipids, cholesterol and by temperature. 3. Identify a molecule as small (1-100 Daltons), medium (100 to 1000 Daltons) or large (> 1000 Daltons) and their polarity to deduce the different ways such molecules can be transported through a lipid bilayer or proteins. Know some specific examples. 4. Define diffusion (simple and facilitated), osmosis, osmoticum, active transport and passive transport. 5. Predict how the solute concentration of the surroundings affects the water movement and structure of an animal or plant cell. 6. Compare and contrast passive and active transport with some examples and situations in which they work. 7. Know examples of uniport, symport, antiport, electrogenic pump and apply the knowledge to given situations and problems. 8. Understand exocytosis and endocytosis; phagocytosis, pinocytosis and receptormediated endocytosis. 9. Predict which of the above mechanisms would be used in a given situation of cell transport based on the size of molecule, concentration gradient and polarity of the molecule. 10. Compare how biological membranes behave differently compared to a dialysis (synthetic) membrane in permeability. 11. Design an experiment to study cell transport with given materials by developing a hypothesis, treatments, observations and results analysis. CELL COMMUNICATION Cell and Molecular Biology - Book Chap. 7 After learning this topic, you should be able to 1. Understand why cells need to communicate with each other and inside them. 2. Differentiate the signal mechanisms that communicate directly, or to nearby cells or far away cells. 3. See the big picture of cell communication starting with signal reception followed by signal transduction and finally cell response. 4. Compare various signals, signal transduction mechanisms and cell or organismal responses. 5. Predict the cascade of cell communication under given situation and diagnose a cellular or tissue defect with given information. How to study for this exam? 1. Read and understand the textbook and the course guide. 2. Organize the content with outlines, concept maps and figures. Incorporate the lecture and discussion notes. 3. If you do not understand a term or concept or how to apply, ask specific questions to the Instructor, TA or in Piazza to clarify. 4. Practice recalling the information with the book and notes closed. 5. Practice applying the information to various situations. 6. Go through old exams, worksheets, in class /discussion section activities, clicker questions and text book questions.