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D (+)-Glucose, anhydrous Cell Culture Tested Product Code: TC130 Product Description : Molecular Weight: 180.16 Molecular Formula: C6H12O6 CAS No.: 50-99-7 Synonym: Dextrose Glucose is a carbohydrate compound consisting of six carbon atoms and an aldehyde group and they are referred to as aldohexose. The glucose structure can exist in an open-chain (acyclic) and ring (cyclic) form. It occurs in many fruits, animal tissues and fluids, etc. Glucose has several optically different forms. The common form is dextrorotatory glucose because of which it is also known as dextrose. All forms of glucose are colourless and soluble in water. Glucose is used an energy source in most of the organisms from bacteria to human beings. In plants and some prokaryotes, glucose is the produced as result of photosynthesis. In animals and fungi, glucose is obtained from the breakdown of glycogen by a process known as glycogenolysis. In animals, it is also synthesized in the liver and kidney by a process known as gluconeogenesis from pyruvate and glycerol. Glucose is used as a major component in wide range of cell culture media including classical and serum-free media. It plays many important roles in cell culture. In vitro functions of glucose: · Energy source: Glucose is utilized as a major source of energy by cultured cells. Several transporter proteins are involved in transport of glucose molecules in and out of the cells via cell membrane. Glucose cannot enter or exit the cells without these proteins. Once inside the cells, it serves as a major substrate in following cell metabolic pathways: 1) Glycolysis 2) Glycogenesis 3) Pentose-phosphate pathway 4) Citric acid cycle · Cell defense: In addition to its role in cell proliferation and cell metabolism, glucose is also involved in maintaining reduced environment inside the cells. Metabolism of glucose via the oxidative branch of the pentose phosphate pathway provides the reducing power needed to maintain the pool of NADPH. Cells in vitro are subjected to oxidative stress and their ability to survive and grow is likely to be significantly affected by their capacity to generate NADPH through the PPP. Applications: · In bio-manufacturing: Glucose is one of the major components of nutritional supplement solutions used in fed-batch biomanufacturing of recombinant proteins, monoclonal antibodies, viral vectors for gene therapy and viral vaccines from mammalian and insect cell cultures. Addition of such glucose containing supplements in mid-run results in improvement in the quantity of the harvested product. · In vitro diabetes research: Glucose is a potent regulator of pancreatic beta cell activity. It stimulates beta- cell proliferation and destruction. Addition of tritiated glucose to the culture medium and studying extent of glucose uptake in in vitro diabetes model is a very widely used system for evaluation of potency of anti-diabetic drug. Directions : Preparation instructions: For cell culture applications, glucose is generally used at a concentration of 1-10g/L depending on type of the medium and cells. Glucose is soluble in water (100mg/ml). Glucose solutions can be sterilized by autoclaving or by filtering through a sterile membrane filter of porosity 0.22 microns or less. Please refer disclaimer overleaf Quality Control: Appearance White powder. Solubility Clear, colorless solution at 10gm in 100ml of water . pH 5.00 - 6.50 Specific rotation α20 D +52.5° to +53.0° Assay NLT 99.00% Cell Culture Test Passes Insect Cell Culture Test Passes Storage and Shelf Life: Store below 15-30°C away from bright light. Shelf life is 48 months. Use before expiry date given on the product label. Revision : 1 / 2013 Disclaimer : User must ensure suitability of the product(s) in their application prior to use. Products conform solely to the information contained in this and other related HiMedia™ publications. The information contained in this publication is based on our research and development work and is to the best of our knowledge true and accurate. HiMedia™ Laboratories Pvt Ltd reserves the right to make changes to specifications and information related to the products at any time. Products are not intended for human or animal diagnostic or therapeutic use but for laboratory, research or further manufacturing use only, unless otherwise specified. Statements contained herein should not be considered as a warranty of any kind, expressed or implied, and no liability is accepted for infringement of any patents. HiMedia Laboratories Pvt. Ltd. A-516,Swastik Disha Business Park,Via Vadhani Ind. Est., LBS Marg, Mumbai-400086, India. Customer care No.: 022-6147 1919 Email: [email protected]