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School of Biomedical, Biomolecular and Chemical Sciences Unit Outline Molecular Biology SCIE2225 Semester: 1 Campus: Crawley and QEII Medical Centre Unit Coordinator: A/Prof Barbara Chang This outline is the currently available version for this unit. Detailed Information on unit content and assessment may undergo modification before the time of delivery of the unit. For the most up to date information students must consult material supplied to enrolled students by the unit co-ordinator. All material reproduced herein has been copied in accordance with and pursuant to a statutory licence administered by Copyright Agency Limited (CAL), granted to the University of Western Australia pursuant to Part VB of the Copyright Act 1968 (Cth). Copying of this material by students, except for fair dealing purposes under the Copyright Act, is prohibited. For the purposes of this fair dealing exception, students should be aware that the rule allowing copying, for fair dealing purposes, of 10% of the work, or one chapter/article, applies to the original work from which the excerpt in this course material was taken, and not to the course material itself © The University of Western Australia 2001 1 Introduction The objectives of this unit are to provide students with a broad knowledge and understanding of the concepts and methods used in molecular biology. Molecular biology can be defined as the study of biological phenomena in molecular terms, or more precisely as the study of gene structure and function at the molecular level. The unit will interest students in many areas of biology who wish to understand the spectacular advances that have recently been made in molecular biology, such as the sequencing of the human, mouse, rice, dog and many other genomes, the simultaneous expression of thousands of genes by DNA chip technology, and many other techniques leading to novel insights into evolution, medical and agricultural science. The unit extends many of the topics introduced in Molecular Biology of the Cell 106. The content of the unit is divided into several major themes: revision of DNA, RNA and protein synthesis; chromosome structure and function; protein structure and function; tools of the molecular biologist; the regulation of gene activity in prokaryotes and eukaryotes; bioinformatics; recombinant DNA technology and genetic engineering. The practical work will familiarise the student with techniques basic to Molecular Biology. Broad Learning Outcomes Outcomes Assessment Activity Students will be able to recall and integrate key knowledge and concepts about: chromosome structure and function protein structure and function tools of the molecular biologist regulation of gene expression in prokaryotes and eukaryotes recombinant DNA technology introductory bioinformatics Theory exam Lab worksheets Students will acquire skills in: polymerase chain reaction plasmid isolation and sizing DNA melting, ligation and hybridization genetic code analysis determination of gene regulation in prokaryotes Lab worksheets Lab quizzes Practical test Advisable Prior Study Molecular Biology of the Cell 106 is a prerequisite unit. Students must also have completed another 6 point level 1 biology unit, plus 12 points of level 1 Chemistry. Technical Requirements All equipment required is provided in the laboratories. Lab coats are provided for use during the practicals held in Microbiology, however a lab coat must be purchased for use in Biochemistry laboratories. 2 Software Requirements Nil Contact Details Unit web site (may include Web CT URL): http://Webct.uwa.edu.au/ Name of Unit coordinator: A/Prof Barbara Chang e-mail: [email protected] Phone: 9346 2288 Fax: 9346 2912 Consultation hours: By appointment Unit Structure Summary Lectures Two 45 minute lectures per week. Practical and/or Laboratory Sessions One 2-hour laboratory class every week. Attendance is compulsory. Tutorials There is time allocated during the laboratory classes for discussion of experimental results and worksheets. Topics For details of times and venues of all teaching sessions please consult the university timetable at http://www.timetable.uwa.edu.au/ LECTURE TOPICS 1 Introduction to unit 2 Revision of basic molecular genetic mechanisms 3 Assumed skills and revision questions 4 Chromosome structure and function: DNA organisation and repair 5 Chromosome structure and function: Structure/function linkage 6 Overview of protein structure and function 7 Purifying, detecting and characterising proteins 8 Tools of the molecular biologist: Plasmids 9 Tools of the molecular biologist: Viruses and transposons 10 Tools of the molecular biologist: Restriction and modification of DNA 11 Recombinant DNA technology: Cloning by PCR 3 LECTURE TOPICS continued 12 Recombinant DNA technology: Genetic recombination and the use of recombinase in cloning 13 Recombinant DNA technology: DNA sequencing and synthesis 14 Regulation of gene expression in prokaryotes: Protein-DNA interactions 15 Regulation of gene expression in prokaryotes: Repressors, activators and global regulatory mechanisms 16 Regulation of gene expression in eukaryotes: Measuring gene expression – Northern blots 17 Regulation of gene expression in eukaryotes: Measuring gene expression – arrays 18 Regulation of gene expression in eukaryotes: Levels of transcriptional control in eukaryotes 19 Regulation of gene expression in eukaryotes: Combinatorial control in transcription 20 Regulation of gene expression in eukaryotes: Global regulation by oxygen in yeasts 21 Regulation of gene expression in eukaryotes: Global regulation by oxygen in mammals and plants 22 Introduction to Bioinformatics 23 Bioinformatic resources and applications 24 Unit review and exam information References, Resources and Reading Materials Recommended Text Molecular Cell Biology, Lodish H. et al., 5th Edition, 2004, Freeman and Co. Reference Books Essentials of Molecular Biology, Malacinski G.M. and Freifelder D., 4th Edition, 2002, Jones and Bartlett Publishers. Genes VIII, Lewin B., 2004, Pearson Prentice Hall Molecular Biology, Weaver R., 2002, 2nd Edition, McGraw-Hill. The text book by Lodish is currently also the text for Molecular Biology 325. It has more depth of information than is necessary for Molecular Biology 225, but is a good buy for students considering further studies in molecular biology. It is available on closed reserve in the Biological Sciences and Medical Libraries. Molecular Biology of the Cell, and Essential Cell Biology, both by Alberts, provide useful supportive material in some areas. These are the texts for Molecular Biology of the Cell 106. All lecturers provide lecture handouts, which will be available on WebCT. The unit laboratory manual, supplied to all students enrolled in the unit, contains detailed instructions, background information and worksheets. Unit web site All enrolled students have access to the unit WebCT site (http://webct.uwa.edu.au). This site will be used for notices, posting of supplementary course materials, email and discussion. It is strongly recommended that you log onto this site on a daily basis. The website should be used for all remote communication to the unit co-ordinator. Students are encouraged to pose questions about course content on the discussion forum of the site so that all class members can view and contribute to the discussion. 4 Assessment Details Assessed Work % Mark Timing Final exam – 2hr exam. Mix of MCQ and short answer questions 70% End of semester exam period Microbiology Laboratory assessment: open book lab quiz 15% Last laboratory session of this section Biochemistry Laboratory assessment: practical test 15% Last laboratory session of this section Plagiarism The University of Western Australia takes very seriously the matter of academic misconduct by students and has policies in place that define misconduct (including plagiarism) and the penalties that apply. The consequences for misconduct can be severe, including exclusion from the university. All students are expected to make themselves aware of the definitions and policies relating to academic misconduct, found at the websites below, and with any additional requirements or stipulations that may be provided by individual unit co-ordinators. http://www.secretariat.uwa.edu.au/__data/page/20839/StuMiscondInfo.rtf http://www.teachingandlearning.uwa.edu.au/tl/academic_conduct When you obtain authority to log in to the unit website, you will se an online module on academic conduct which you are strongly advised to complete in your own time. 5