Download Meiosis II

MEIOSIS
Organisms that reproduce Sexually are
made up of two different types of cells.
1. Somatic Cells are “body” cells and contain the
normal number of chromosomes ….called the
“Diploid” number (the symbol is 2n). Examples
would be … skin cells, brain cells, etc.
2. Gametes are the “sex” cells and contain only half
the normal number of chromosomes…. called the
“Haploid” number (the symbol is n)….. Sperm
cells and ova are gametes.
n = number of chromosomes in the set… so….2n means 2
chromosomes in the set…. Polyploid cells have more than two
chromosomes per set… example: 3n (3 chromosomes per set)
Gametes
• The Male Gamete is the Sperm
• The Female Gamete is the Ovum
Fertilization, the joining of the sperm
and ovum ,results in the formation of
the zygote. (fertilized egg)
n=23
egg
(haploid)
sperm
(haploid)
n=23
2n=46
zygote
(diploid)
REVIEW TIME!
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What is a gamete?
What is the male gamete called?
What is the female gamete called?
What happens during fertilization?
What is a zygote?
Which of the following are haploid: egg
sperm, zygote?
• Which of the following are diploid: egg
sperm, zygote?
Summary of Mitosis…
• Mitosis Review
Chromosomes
• If an organism has the diploid number (2n) it has
a matching pair of homologous chromosomes for
each chromosome number. One of the
homologues comes from the mother (and has the
mother’s DNA).… the other homologue comes
from the father (and has the father’s DNA).
• Most organisms are diploid. Humans have 23 sets
of chromosomes… therefore humans have 46
total chromosomes….. The diploid number for
humans is 46 (46 chromosomes per cell).
Basic Genetic Information
• Genes are located on
chromosomes
• Alleles are alternate
forms of a gene
• EX: The gene for eye
color is on a
chromosome, it can
have several different
alleles: blue, brown,
green
Homologous Chromosomes
• Pairs of chromosomes (maternal and paternal) that
are similar in shape and size.
• Homologous pairs (tetrads) carry genes controlling
the same inherited traits.
• Each locus (position of a gene) is in the same
position on homologues.
• Humans have 23 pairs of homologous chromosomes.
22 pairs of autosomes
1 pair of sex chromosomes
Homologous Chromosomes
(because
a homologous pair consists of 4 chromatids it is called a “Tetrad”)
*May not always be identical because alleles can be different
eye color
locus
eye color
locus
(Brown)
(Blue)
hair color
locus
hair color
locus
(Brown)
(Blonde)
Paternal
Maternal
Humans have 23 Sets of Homologous Chromosomes
Each Homologous set is made up of 2 Homologues.
Homologue
Homologue
Autosomes
The Autosomes code for most of the
offspring’s traits
In Humans the
“Autosomes”
are sets 1 - 22
Sex Chromosomes
The Sex Chromosomes code for the sex of the offspring.
If the offspring has two “X” chromosomes it will be a female.
If the offspring has one “X” chromosome and one “Y”
chromosome it will be a male.
In Humans the
“Sex
Chromosomes”
are the 23rd set
XX chromosome - female
XY chromosome - male
Sex Chromosomes
“Sex Chromosomes”
…….the 23rd set
This person has 2
“X” chromosomes…
and is a female.
23
REVIEW TIME!
• How many pairs of chromosomes do humans
have?
• Pairs 1-22 are called what?
• Pair 23 is called what?
• Females have what chromosomes?
• Males have what chromosomes?
• Where are genes located?
• Are the homologous chromosomes exactly
the same?
• Are sister chromatids exactly the same?
• What is an allele?
Meiosis
is the process by which “gametes” (sex cells) , with half
the number of chromosomes, are produced.
During Meiosis diploid cells are reduced to haploid cells
Diploid (2n)

Haploid (n)
If Meiosis did not occur the chromosome
number in each new generation would
double…. The offspring would die.
Meiosis
Meiosis is two
cell divisions
(called meiosis I
and meiosis II)
with only one
duplication of
chromosomes.
Meiosis in males is called
spermatogenesis and produces sperm.
Meiosis in females is called oogenesis
and produces ova.
Spermatogenesis
Secondary Spermatocyte
n=23
human
sex cell
2n=46
sperm
n=23
Primary Spermatocyte
n=23
Secondary Spermatocyte
haploid (n)
n=23
diploid (2n)
n=23
4 sperm cells are
produced from each
primary spermatocyte.
meiosis I
n=23
meiosis II
Oogenesis
*** The polar bodies die… only one ovum
(egg) is produced from each primary oocyte.
REVIEW TIME!
• What is the purpose of meiosis?
• How many cells are produced as a result
of meiosis (haploid or diploid)?
• Why do we need to reduce the number of
chromosomes?
• How many cell divisions does meiosis go
through?
• In males, what is produced as a result of
meiosis?
• In females, what is produced as a result of
meiosis?
Interphase I
• Similar to mitosis interphase.
• Chromosomes replicate (S phase).
• Each duplicated chromosome consists of two
identical sister chromatids attached at their
centromeres.
• Centriole pairs also replicate.
Interphase I
• Nucleus and nucleolus visible.
chromatin
nuclear
membrane
cell membrane
nucleolus
Meiosis I (four phases)
• Cell division that reduces the chromosome
number by one-half.
• four phases:
a. prophase I
b. metaphase I
c. anaphase I
d. telophase I
Prophase I
• Longest and most complex phase.
• 90% of the meiotic process is spent in Prophase I
• Spindle fibers form, centrioles move to the poles and
nuclear envelope dissolves.
• Chromatin condenses into replicated chromosomes
(2 sister chromatids).
• As homologous chromosomes condense, synapsis
occurs: homologous chromosomes come together
Prophase I - Synapsis
Homologous chromosomes
sister chromatids
Tetrad
sister chromatids
During synapsis (in Prophase I)
“Crossing Over” occurs.
Crossing Over is one of the Two major
occurrences of Meiosis
(The other is Non-disjunction)
• During Crossing over segments of
chromosomes break off and reattach to
the paired homologous chromosome. This
leads to greater genetic diversity!
Crossing Over
creates variation (diversity) in the offspring’s traits.
nonsister chromatids
chiasmata: site
of crossing over
Tetrad
genetic
variation
Question:
• A cell containing 20 chromosomes (diploid)
at the beginning of meiosis would, at its
completion, produce cells containing how
many chromosomes?
Answer:
• 10 chromosomes (haploid)
Prophase I
spindle fiber
aster
fibers
centrioles
REVIEW TIME!
• Draw a picture of what Prophase I looks
like.
• What is crossing over?
• Why is it important?
• What phase does the cell spend most
of its life in (meiosis, NOT mitosis!)
Metaphase I
• Shortest phase
• Spindle fibers attach to the centromere of each
homologous chromosome
• Pairs of homologous chromosomes line up at
the equator of the cell.
This is a major difference
between Meiosis and Mitosis.
Recall in Mitosis, individual
chromosomes line up at the
equator.
Metaphase I
• INDEPENDENT ASSORTMENT OCCURS:
1. Orientation of homologous pair to poles is random.
2. Leads to greater genetic variation
Metaphase I
Anaphase I
• Homologous chromosomes separate and move
towards the poles.
• Sister chromatids remain attached at their
centromeres.
Anaphase I
Telophase I
• Chromosomes uncoil and spindle
fibers break down. Nuclear
envelopes form around the DNA at
each pole creating 2 nuclei.
• Each pole now has one of the 2
homologous chromosomes
consisting of 2 sister chromatids.
The sister chromatids may not be
identical due to crossing over.
Telophase I
• Cytokinesis occurs and two haploid daughter
cells are formed. Because there is only one
copy of each chromosome at each pole, they
are said to be haploid.
Telophase I
Cleavage Furrow
Meiosis II
• No interphase II
(or very short period called interkinesis –
no DNA replication occurs)
Meiosis II is similar to mitosis
Prophase II
• same as prophase in mitosis
Metaphase II
• same as metaphase in mitosis
Anaphase II
• same as anaphase in mitosis
• sister chromatids separate
Telophase II
• Same as telophase in mitosis.
• Nuclei form.
• Cytokinesis occurs.
• Remember:
four haploid daughter cells
produced.
gametes = sperm or egg
Telophase II
REVIEW TIME!
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Draw a picture of anaphase I.
Draw a picture of telophase I.
Draw a picture of metaphase II.
Draw a picture of anaphase II.
Draw a picture of telophase II.
How many cells are produced as a
result of meiosis?
• Haploid or diploid?
Meiosis Review
• Meiosis Summary
Non-disjunction
Non-disjunction is one of the Two major occurrences of Meiosis
(The other is Crossing Over)
• Non-disjunction is the failure of homologous
chromosomes, or sister chromatids, to
separate during meiosis.
• Non-disjunction results in the production of
zygotes with abnormal chromosome
numbers…… remember…. An abnormal
chromosome number (abnormal amount of
DNA) is damaging to the offspring.
Non-disjunctions is displayed in
one of two fashions.
• The first is called Monosomy, the second
is called Trisomy. If an organism has
Trisomy 18 it has three chromosomes in
the 18th set, Trisomy 21…. Three
chromosomes in the 21st set. If an
organism has Monosomy 23 it has only
one chromosome in the 23rd set.
Common Non-disjunction Disorders
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Down’s Syndrome – Trisomy 21
Turner’s Syndrome – Monosomy 23 (X)
Kleinfelter’s Syndrome – Trisomy 23 (XXY)
Edward’s Syndrome – Trisomy 18
Amniocentesis
• An amniocentesis is a procedure a
pregnant woman can have in order to
detect some genetics disorders…..such as
non-disjunction.
Amniocentesis
Amniotic fluid withdrawn
Karyotype
(picture of an individual’s chromosomes)
One of the ways
to analyze the
amniocentesis
is to make a
Karyotype
What genetic
disorder does
this karyotype
show?
Trisomy
21….Down’s
Syndrome
REVIEW TIME!
• What is nondisjunction?
• What is it called when you have twice
the amount of genetic material?
• What is it called when you have half the
amount of genetic material?
• What is amniocentesis?
• What is a karyotype, and why are they
important?