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Download Build a Paper Model of Transfer RNA (tRNA)
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Build a Paper Model of Transfer RNA (tRNA) pdb101.rcsb.org I. The primary structure bases phosphates 1 Cut out each piece, leaving the grey tabs intact. sugars nucleotide numbering phosphodiester backbone 2 Following the nucleotide numbering, tape the pieces into a long strip. The blank grey tabs should be hidden. 3 Make slits in-between each double line (16 total). Be careful not to cut through the entire strip. 4 Fold back ("mountain fold") on the horizontal dashed lines II. The secondary structure Acceptor arm TΨC arm Anticodon arm Anticodon Acceptor Fold in ("valley fold") on the small vertical dotted lines. 6 Bring colored/patterned sections together, and tape so the grey tab is hidden. It is important that you match the bases in the following order: 1. 10-13 with 25-22 2. 27-31 with 43-39 3. 49-53 with 65-61 4. 1-7 with 72-66 or or or or Notice the almost-perfect base pairing (G:C and A:U bases pair up). At this point the model is a cloverleaf shape– the secondary structure of tRNA. Each colored region represents the double helical regions of the structure. What is tRNA? III. The tertiary structure 7 Bring together bases G19 and C56 (colored yellow) to form a base pair – hide the grey tab by taping it under. This forms the beginnings of the tRNA tertiary structure– the inverted L-shape. Transfer RNA (tRNA) "translates" the genetic code into the language of proteins. Each tRNA molecule binds to a specific amino acid on the acceptor arm, recognizes its corresponding code on the mRNA through the anticodon loop region and delivers the amino acid to a growing peptide chain in the ribosome for protein synthesis. Go to pdb101.rcsb.org to: n 6 y Anticodon 5 t D-arm & Acceptor Arm Anticodon Loop • READ the Molecule of the Month articles on tRNA and Ribosomes 3D • DOWNLOAD additional copies of this model, WATCH a video demonstration of how to build it, and to access the DIGITAL ACTIVITY PAGE allowing for futher exploaration of the 3D model (Learn > Paper Models) PDB-101 is the educational portal of RCSB Protein Data Bank (rcsb.org) tRNA Model Template for Reproduction in Black pdb101.rcsb.org pdb101.rcsb.org PDB-101 is the educational portal of RCSB Protein Data Bank (rcsb.org) tRNA Model Template for Reproduction in Color pdb101.rcsb.org pdb101.rcsb.org PDB-101 is the educational portal of RCSB Protein Data Bank (rcsb.org)