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DO NOT WRITE ON THIS LAB !!!!! LAB # ______ Lab Instructions – Translation Please Purpose: To help students understand the role of DNA, mRNA, tRNA, and amino acids in the role of protein synthesis. This activity will also introduce the concept of mutations. Procedure: 1. You will be working in 3 person teams. 2. The teacher’s desk is the nucleus and the DNA templates cannot leave that area. The teacher will designate one team member’s desk for each group as the ribosome. The mRNA must be read at this station. 3. Students have 3 jobs. One student will be the mRNA and transcribe the DNA at the teacher’s desk. 4. With the mRNA sequence she/he will go back to the group’s desk and the ribosomal student will write out the tRNA anti-codon sequence for the tRNA. 5. The tRNA student will go out to the cell (walls of the classroom) and will search out the correct anti-codon card and flip the card over revealing the word. He/she will write down the word and bring it back to the ribosome. 6. The ribosomal student will assemble the amino acids (words) into a protein (sentence). 7. This will continue until the sentence is complete. 8. If students incorrectly transcribe the DNA strand or the mRNA strand, then a mutation will occur and the sentence will not make any sense. 9. Read the teacher your sentence and get another DNA template until you have completed the required number of “proteins”.