Download Somatic Cell Nuclear Transfer (SCNT) - bli-research-synbio

Somatic Cell Nuclear Transfer
(SCNT)
By Caroline Kim
DOLLY THE SHEEP AND
HER FIRST LAMB
CALLED BONNY
What is SCNT?
 Somatic Cell

A somatic cell is any cell in the body other than the reproductive cells
 Nuclear

The nucleus is a membrane bound organelle found in eukaryotic cells
that contains most of the cell’s genetic material
 Transfer

To move from one place to another
Stages of Fertilization
Embryonic Stem Cells
Cloning
 Most practical use of SCNT is to clone farm animals

Milk

Eggs

Meat

Pigs-organ donors
Dolly (6LL3)
(July 5, 1996-February 14, 2003)
 First mammal to be cloned
from an adult somatic cell
through SCNT at Roslin
Institute in Scotland
 The donor nucleus was not
injected into the oocyte,
but fused by an electric
current
DOLLY THE SHEEP AND HER FIRST
LAMB CALLED BONNY
Dolly (6LL3)
(July 5, 1996-February 14, 2003)
 Scientists at Roslin believe that Dolly died so young
(6 years old) due to the age of the sheep she was
cloned from (6 years old).
 Believed she may have been “old at birth”

Telomeres were shorter than expected for a sheep her age
Bibliography
http://www.sciencedaily.com/articles/d/dolly_the_sheep.htm
http://www.ncbi.nlm.nih.gov/pubmed/12006157
http://www.biologyjunction.com/images/scnt.jpg
http://www.roslin.ed.ac.uk/public-interest/dolly-the-sheep/technical-aspects-of-cloning/
http://www.cogforlife.org/scnt.htm
http://learn.genetics.utah.edu/content/cloning/whatiscloning
/http://www.nature.com/nature/journal/v487/n7405/full/487043a.html?message-global=remove
http://www.biologyjunction.com/stemcell_article.htm