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Meiosis and Biotechnology Review Guide I. Vocabulary Cell cycle Chromosome Cytokinesis Gene G2 Phase Stem cell Mitosis Meiosis Centrioles Cell Plate Centromere Somatic cell Crossing over Tetrad Haploid (n) S phase DNA Fingerprinting Chromatid Sister chromatids Gametes Spindle fibers Cloning Chromatin Diploid (2N) G1 Phase Genetic Recombination PCR II. Short Answer 1) 2) 3) 4) 5) 6) Why is it important that cells divide and not continuously grow? What type of cell is incapable of repairing itself once it reaches maturity? What is cell division and why is it important? Describe the structure of sister chromatids? Describe what occurs during Interphase. What purpose do the spindle fibers serve in cell division? What structure makes them in the animal cell? Plant cell? 7) Draw the stages of Meiosis for an animal cell: 2N=2. 8) Explain crossing over and how a tetrad is involved? 9) How does the cell regulate the cell cycle? 10) What occurs during Pro I of Meiosis? What must occur first? 11) Differentiate between spermatogenesis and oogenesis. What is the basic differenceinclude number of cells and types of cells created? 12) Describe the differences between Meiosis I and Meiosis II. 13) What is a karyotype? 14) Be prepared to review karyotypes to determine gender and normal vs. abnormal For Questions 13-21 Use your Biotechnology/Cloning Web quest worksheet 15) What causes DNA to move through a gel in Gel electrophoresis? 16) What is the gel used in Gel Electrophoresis made of? 17) Do shorter or longer strands of DNA move farther in gel during Gel Electrophoresis? 18) Why is it necessary to stain the gel? See web quest for help. 19) Explain the process of cloning? 20) Review the DNA fingerprint below and determine who committed the crime. 21) Try another DNA fingerprinting problem. 22.) Identify what stages of Meiosis are shown below. What can occur during this stage? A. B. 23.) Explain what is occurring below. What phase of Meiosis does this occur in?