Laboratory manual for the diagnosis of whooping cough caused by... pertussis/ Bordetella parapertussis
... against severe disease and begins to wane after about three years. Acellular pertussis vaccines do not protect against infection by B. parapertussis. The need and timing for additional booster doses of DTP and their efficacy should be assessed by national programs. In the USA, booster doses are reco ...
... against severe disease and begins to wane after about three years. Acellular pertussis vaccines do not protect against infection by B. parapertussis. The need and timing for additional booster doses of DTP and their efficacy should be assessed by national programs. In the USA, booster doses are reco ...
MCB317 Topic 10, part 4, A Story of Txn Sp14
... Secondary screen to identify possible txn factors ...
... Secondary screen to identify possible txn factors ...
Tamoxifen or Raloxifene in Postmenopausal Women for Prevention
... carcinoma in situ by 37% to 49% compared with placebo (10, 17). Raloxifene has not been compared with a placebo in high-risk women, but in average to low-risk postmenopausal women, raloxifene was not associated with either a significant or numerical reduction in ductal carcinoma in situ compared wit ...
... carcinoma in situ by 37% to 49% compared with placebo (10, 17). Raloxifene has not been compared with a placebo in high-risk women, but in average to low-risk postmenopausal women, raloxifene was not associated with either a significant or numerical reduction in ductal carcinoma in situ compared wit ...
Plant centromeres: structure and control Eric J Richards and R Kelly
... itive DNA [18]. It is likely that this block corresponds, on the cytological level, to the heterochromatin that encompasses the region associated with the kinetochore. This pericentric heterochromatin appears to have a role in controlling and/or mediating sister chromatid cohesion in metaphase (of m ...
... itive DNA [18]. It is likely that this block corresponds, on the cytological level, to the heterochromatin that encompasses the region associated with the kinetochore. This pericentric heterochromatin appears to have a role in controlling and/or mediating sister chromatid cohesion in metaphase (of m ...
Erp, an extracellular protein family specific to
... factor in Mycobacterium tuberculosis and was thought to be present only in Mycobacterium leprae and members of the TB complex. Here it is shown that Erp is a ubiquitous extracellular protein found in all of the mycobacterial species tested. Erp proteins have a modular organization and contain three ...
... factor in Mycobacterium tuberculosis and was thought to be present only in Mycobacterium leprae and members of the TB complex. Here it is shown that Erp is a ubiquitous extracellular protein found in all of the mycobacterial species tested. Erp proteins have a modular organization and contain three ...
Dynamic Model of Paclitaxel Biosynthesis Suggests That the Key
... specially effective against breast cancer and non-small cell lung cancer [1].The most promising and environmentally friendly way for the sustainable production of paclitaxel is provided by plant cell cultures. Though more than 20 molecular cloning of cDNA sequences encoding these enzymes have been s ...
... specially effective against breast cancer and non-small cell lung cancer [1].The most promising and environmentally friendly way for the sustainable production of paclitaxel is provided by plant cell cultures. Though more than 20 molecular cloning of cDNA sequences encoding these enzymes have been s ...
DNA breathing dynamics distinguish binding from nonbinding
... LMD simulations distinguish true YY1 binding from nonbinding sites in the human PLG promoter YY1 knockdown in HeLa cells coincides with the accumulation of plasminogen (PLG) mRNA (not shown). The gene product regulates a wide variety of biologic responses directly related to the development of cardi ...
... LMD simulations distinguish true YY1 binding from nonbinding sites in the human PLG promoter YY1 knockdown in HeLa cells coincides with the accumulation of plasminogen (PLG) mRNA (not shown). The gene product regulates a wide variety of biologic responses directly related to the development of cardi ...
Structural organization of the transfer RNA gene clusters of cholera
... phage φ 149 genome. The results presented here show that the tRNA genes are contained in two HindIII fragments, 11 and 3·4 kb in length. These two fragments do not bear any sequence homology and the smallest fragment alone carried the genes for tRNAs specific for nine different amino acids. Secondly ...
... phage φ 149 genome. The results presented here show that the tRNA genes are contained in two HindIII fragments, 11 and 3·4 kb in length. These two fragments do not bear any sequence homology and the smallest fragment alone carried the genes for tRNAs specific for nine different amino acids. Secondly ...
Rapid and accurate cancer somatic mutation
... of a low percentage of mutant cells among non-malignant cells ...
... of a low percentage of mutant cells among non-malignant cells ...
Epigenetic inheritance of acquired traits through sperm RNAs and
... axin fused (AxinFu)35 gene activity, in which the methy lation state of the IAP was incompletely erased during reprogramming of the male germ line, providing an explanation for the epigenetic inheritance of coat colour or kinky-tail phenotype, respectively. However, such clearly identified inherit ...
... axin fused (AxinFu)35 gene activity, in which the methy lation state of the IAP was incompletely erased during reprogramming of the male germ line, providing an explanation for the epigenetic inheritance of coat colour or kinky-tail phenotype, respectively. However, such clearly identified inherit ...
Mating-Type Genes From the Homothallic Fungus Sordaria
... with the N. crassa A idiomorph. This similarity is markedly reduced further downstream from position 4860 of the S. macrospora sequence. The 3' sequence of SmtA3 reveals a significant homology with the N. crassa a idiomorph (Figure 3B). SmtA-3 is interrupted by one intron of 54 bp andencodes apolype ...
... with the N. crassa A idiomorph. This similarity is markedly reduced further downstream from position 4860 of the S. macrospora sequence. The 3' sequence of SmtA3 reveals a significant homology with the N. crassa a idiomorph (Figure 3B). SmtA-3 is interrupted by one intron of 54 bp andencodes apolype ...
Transcripts of the MHM region on the chicken Z chromosome
... The male hypermethylated (MHM) region, located near the middle of the short arm of the Z chromosome of chickens, consists of approximately 210 tandem repeats of a BamHI 2.2-kb sequence unit. Cytosines of the CpG dinucleotides of this region are extensively methylated on the two Z chromosomes in the ...
... The male hypermethylated (MHM) region, located near the middle of the short arm of the Z chromosome of chickens, consists of approximately 210 tandem repeats of a BamHI 2.2-kb sequence unit. Cytosines of the CpG dinucleotides of this region are extensively methylated on the two Z chromosomes in the ...
Maintenance of genomic integrity by p53: complementary
... to localize the junction of incised DNA, but is not needed during DNA synthesis (Li et al., 1994a). Thereby, repair DNA synthesis could proceed also in the presence of an upregulated p21Waf1. G2 arrest The third and the last line of defense against induced chromatid damage occurs in G2. A possible r ...
... to localize the junction of incised DNA, but is not needed during DNA synthesis (Li et al., 1994a). Thereby, repair DNA synthesis could proceed also in the presence of an upregulated p21Waf1. G2 arrest The third and the last line of defense against induced chromatid damage occurs in G2. A possible r ...
the association of chloroplast dna with photosynthetic membrane
... in the electron microscope they appear as in Figs. 6-8. They are bound by a membrane to which adhere varying numbers of grana lamellae. The number of grana lamellae seen in a single section will vary according to the section plane. The grana are partially disrupted due to the isolation in the 3-5 mM ...
... in the electron microscope they appear as in Figs. 6-8. They are bound by a membrane to which adhere varying numbers of grana lamellae. The number of grana lamellae seen in a single section will vary according to the section plane. The grana are partially disrupted due to the isolation in the 3-5 mM ...
Synthetic Chemical Inducers and Genetic
... bacterial cells, unlike the inducers of some other systems such as tetracycline, the lactose analogue isopropyl β-D-1-thiogalactopyranoside (IPTG)23,25−27 and in certain cases, arabinose.28 In E. coli the genes encoding the enzymes required for Lrhamnose metabolism (rhaBAD) and the regulatory protei ...
... bacterial cells, unlike the inducers of some other systems such as tetracycline, the lactose analogue isopropyl β-D-1-thiogalactopyranoside (IPTG)23,25−27 and in certain cases, arabinose.28 In E. coli the genes encoding the enzymes required for Lrhamnose metabolism (rhaBAD) and the regulatory protei ...
Part III: Laboratory – Electrophoresis
... AGO1 and thus to locate the gene. The F2 plants that are homozygous for the mutation of interest (+/+), and thus showing the mutant phenotype, will be used for mapping. Since both of their chromosomes contain the mutation and the mutation was from a Ler background, the number of crossing-over events ...
... AGO1 and thus to locate the gene. The F2 plants that are homozygous for the mutation of interest (+/+), and thus showing the mutant phenotype, will be used for mapping. Since both of their chromosomes contain the mutation and the mutation was from a Ler background, the number of crossing-over events ...
SNP
... alleles (0.1% of total SNPs is tri-allelic markers in TSC data). SNPs has lower mutation rate than do repeat sequences, but not as informative as microsatellite markers. detection methods for SNPs are potentially more suitable for genetic screening in automated and large-scale. the SNPs are likely b ...
... alleles (0.1% of total SNPs is tri-allelic markers in TSC data). SNPs has lower mutation rate than do repeat sequences, but not as informative as microsatellite markers. detection methods for SNPs are potentially more suitable for genetic screening in automated and large-scale. the SNPs are likely b ...
MicroRNA Analysis
... Age-Related Diseases. Evidence is accumulating that many age-related diseases are associated with a decreased control of cell signaling that occurs in mid-life. The miRNA control of such systems as the cell cycle, DNA repair, oxidative stress responses and apoptosis, has been shown to become abnorma ...
... Age-Related Diseases. Evidence is accumulating that many age-related diseases are associated with a decreased control of cell signaling that occurs in mid-life. The miRNA control of such systems as the cell cycle, DNA repair, oxidative stress responses and apoptosis, has been shown to become abnorma ...
Obligate phototrophy in cyanobacteria: more than a lack of sugar
... 3.1. Search for glcP homologous genes in various cyanobacteria The strains tested (Table 1) were chosen upon two criteria, their (in)capacity of heterotrophic growth on glucose and when known of glucose uptake, and a G+C content as close as possible to that of Synechocystis PCC6803 [15,16]. Total DN ...
... 3.1. Search for glcP homologous genes in various cyanobacteria The strains tested (Table 1) were chosen upon two criteria, their (in)capacity of heterotrophic growth on glucose and when known of glucose uptake, and a G+C content as close as possible to that of Synechocystis PCC6803 [15,16]. Total DN ...
Characterisation of marsupial PHLDA2 reveals eutherian specific acquisition of imprinting Open Access
... Background: Genomic imprinting causes parent-of-origin specific gene expression by differential epigenetic modifications between two parental genomes. We previously reported that there is no evidence of genomic imprinting of CDKN1C in the KCNQ1 domain in the placenta of an Australian marsupial, the ...
... Background: Genomic imprinting causes parent-of-origin specific gene expression by differential epigenetic modifications between two parental genomes. We previously reported that there is no evidence of genomic imprinting of CDKN1C in the KCNQ1 domain in the placenta of an Australian marsupial, the ...
Restriction Fragment Length Polymorphism of hsp70
... this polymorphism is illustrated in Figure 1. Both human hsp70 probes (2.78 kb and 1.2 kb) and the mouse hsp68 probe gave the same results. These data demonstrate the homogeneity of this gene polymorphism in hypertensive strains of various origins and the presence of larger fragments in both normote ...
... this polymorphism is illustrated in Figure 1. Both human hsp70 probes (2.78 kb and 1.2 kb) and the mouse hsp68 probe gave the same results. These data demonstrate the homogeneity of this gene polymorphism in hypertensive strains of various origins and the presence of larger fragments in both normote ...
Antioxidant and Xenobiotic-metabolizing Enzyme Gene Expression
... particularly the pH 6 optimum reducÃ-asetermed carbonyl reThe clinically useful antineoplastic agent doxorubicin is a ductase (13-16). It has been proposed that decreased enzyme multifunctional molecule, with several possible mechanisms of activity could diminish doxorubicin sensitivity (17). Carbon ...
... particularly the pH 6 optimum reducÃ-asetermed carbonyl reThe clinically useful antineoplastic agent doxorubicin is a ductase (13-16). It has been proposed that decreased enzyme multifunctional molecule, with several possible mechanisms of activity could diminish doxorubicin sensitivity (17). Carbon ...
Fig 16.12a - McGraw Hill Higher Education
... Example: a2 repressor in yeast • Determines mating type by binding to enhancers of certain genes In a haploids and a/a diploids, a2 binds to enhancers of a-specific genes but not to enhancers of haploid-specific genes ...
... Example: a2 repressor in yeast • Determines mating type by binding to enhancers of certain genes In a haploids and a/a diploids, a2 binds to enhancers of a-specific genes but not to enhancers of haploid-specific genes ...
Expression of the six chromate ion transporter
... universal primers. DNA fragments containing the chr genes were obtained by digestions with HindIII/XbaI or HindIII/EcoRI endonucleases and subcloned into the corresponding sites of pACYC184 or pUCP20 vectors. E. coli W3110 cells were transformed by electroporation with recombinant plasmids and trans ...
... universal primers. DNA fragments containing the chr genes were obtained by digestions with HindIII/XbaI or HindIII/EcoRI endonucleases and subcloned into the corresponding sites of pACYC184 or pUCP20 vectors. E. coli W3110 cells were transformed by electroporation with recombinant plasmids and trans ...
Cancer epigenetics
Cancer epigenetics is the study of epigenetic modifications to the genome of cancer cells that do not involve a change in the nucleotide sequence. Epigenetic alterations are as important as genetic mutations in a cell’s transformation to cancer, and their manipulation holds great promise for cancer prevention, detection, and therapy. In different types of cancer, a variety of epigenetic mechanisms can be perturbed, such as silencing of tumor suppressor genes and activation of oncogenes by altered CpG island methylation patterns, histone modifications, and dysregulation of DNA binding proteins. Several medications which have epigenetic impact are now used in several of these diseases.