BLOTTING.142
... Size-separation of the fragments (standard agarose gel electrophoresis) In situ denaturation of the DNA fragments (by incubation @ ↑temp) Transfer of denatured DNA fragments into a solid support (nylon or ...
... Size-separation of the fragments (standard agarose gel electrophoresis) In situ denaturation of the DNA fragments (by incubation @ ↑temp) Transfer of denatured DNA fragments into a solid support (nylon or ...
Evidence that MEK1 positively promotes
... first meiotic non-disjunction. In some organisms the mechanisms ensuring that sufficient DSBs are repaired using crossovers could involve stochastic processes. For example, in Schizosaccharomyces pombe there is evidence that recombination intermediates [joint molecules (JMs)] are more abundant among si ...
... first meiotic non-disjunction. In some organisms the mechanisms ensuring that sufficient DSBs are repaired using crossovers could involve stochastic processes. For example, in Schizosaccharomyces pombe there is evidence that recombination intermediates [joint molecules (JMs)] are more abundant among si ...
High-Level Expression in Escherichia coli of Alkaline Phosphatase
... decreased drastically (data not shown). The recombinant Tca APase activity was activated by Mg2+; however, it was inhibited by Ca2+, Co2+, Zn2+, and EDTA at 1 mM concentration (data not shown). This dependence of the catalytic activity on Mg2+ has already been observed in most of the APases investig ...
... decreased drastically (data not shown). The recombinant Tca APase activity was activated by Mg2+; however, it was inhibited by Ca2+, Co2+, Zn2+, and EDTA at 1 mM concentration (data not shown). This dependence of the catalytic activity on Mg2+ has already been observed in most of the APases investig ...
Risk of Gastric Cancer in Hereditary Nonpolyposis Colorectal
... According to a series of reports on family G, the first HNPCC family described (thus the period of investigation is long and extends for several generations), gastric cancer was the most common cancer at the time of the initial description when the incidence of gastric cancer was extremely high in t ...
... According to a series of reports on family G, the first HNPCC family described (thus the period of investigation is long and extends for several generations), gastric cancer was the most common cancer at the time of the initial description when the incidence of gastric cancer was extremely high in t ...
Synapsis-Mediated Fusion of Free DNA Ends Forms Inverted Dimer Plasmids in Yeast.
... recombination. Homologous recombination provides an avenue by which to restore a broken chromosome to the wild-type structure. The idea that free DNA ends can serve as initiators in homologous recombination has received strong experimental support (reviewed in THALER and STAHL1988). Rearrangement mi ...
... recombination. Homologous recombination provides an avenue by which to restore a broken chromosome to the wild-type structure. The idea that free DNA ends can serve as initiators in homologous recombination has received strong experimental support (reviewed in THALER and STAHL1988). Rearrangement mi ...
Archives of Microbiology
... V-dependent enzyme (V-nitrogenase) with an FeVcofactor under Mo-deWcient growth conditions. In some strains, an Fe-only nitrogenase is active when both Mo and V are unavailable (Loveless and Bishop 1999). The three types of nitrogenase are encoded by separate gene clusters: nifHDK coding for the Mo- ...
... V-dependent enzyme (V-nitrogenase) with an FeVcofactor under Mo-deWcient growth conditions. In some strains, an Fe-only nitrogenase is active when both Mo and V are unavailable (Loveless and Bishop 1999). The three types of nitrogenase are encoded by separate gene clusters: nifHDK coding for the Mo- ...
Transposon stability and a role for conjugational transfer in adaptive mutability
... revertant colonies that accumulate during selection with and without an imposed requirement for conjugal transfer. This loss was found many orders of magnitude more frequently than had been observed for spontaneous transposon loss in the past (16–21). Moreover, even lacI33 Lac⫺ cells, products of co ...
... revertant colonies that accumulate during selection with and without an imposed requirement for conjugal transfer. This loss was found many orders of magnitude more frequently than had been observed for spontaneous transposon loss in the past (16–21). Moreover, even lacI33 Lac⫺ cells, products of co ...
Two-Component System of Mycobacterium Tuberculosis
... the histidine protein kinase (HK) PhoR, are involved in the induction of Pho-regulon genes including the phoPR operon and genes encoding the major vegetative alkaline phosphatases, phoA and phoB. The PhoP protein belongs to OmpR/PhoB subfamily, which is considered as the largest of the response regu ...
... the histidine protein kinase (HK) PhoR, are involved in the induction of Pho-regulon genes including the phoPR operon and genes encoding the major vegetative alkaline phosphatases, phoA and phoB. The PhoP protein belongs to OmpR/PhoB subfamily, which is considered as the largest of the response regu ...
Gene Section PYY (peptide YY) Atlas of Genetics and Cytogenetics
... vitamin E exhibit a significantly increased inhibitory effect on pancreatic cancer in vitro. ...
... vitamin E exhibit a significantly increased inhibitory effect on pancreatic cancer in vitro. ...
7nQ Jj I f NO "7^07 - UNT Digital Library
... to understand. It offers a detailed look at all the components of genetics. It contains study questions as well as summaries for each chapter to provide an even better way to retain the knowledge from my lectures. The course that I have outlined is intended for a junior or senior college student. So ...
... to understand. It offers a detailed look at all the components of genetics. It contains study questions as well as summaries for each chapter to provide an even better way to retain the knowledge from my lectures. The course that I have outlined is intended for a junior or senior college student. So ...
Test Info Sheet
... 4. Prenatal diagnosis for known familial mutation(s) in at-risk pregnancies Methods: Using genomic DNA, coding exons and flanking splice junctions of the genes on this panel are enriched using a proprietary targeted capture method developed by GeneDx. The products are sequenced on an Illumina instru ...
... 4. Prenatal diagnosis for known familial mutation(s) in at-risk pregnancies Methods: Using genomic DNA, coding exons and flanking splice junctions of the genes on this panel are enriched using a proprietary targeted capture method developed by GeneDx. The products are sequenced on an Illumina instru ...
Translational Repression of C. elegans p53 by GLD
... apoptosis phenotype and, conversely, a transgene expressing wild-type GLD-1 rescuing the excess apoptosis phenotype (Supplemental Note 2 and Supplemental Figure S1b). GLD-1 has been shown to bind and translationally repress a number of target mRNAs and is required for multiple aspects of germline de ...
... apoptosis phenotype and, conversely, a transgene expressing wild-type GLD-1 rescuing the excess apoptosis phenotype (Supplemental Note 2 and Supplemental Figure S1b). GLD-1 has been shown to bind and translationally repress a number of target mRNAs and is required for multiple aspects of germline de ...
Myriad--Ambry -- Final Version of Ambry Preliminary Injunction
... sequence similarity with any particular gene requires an application of detailed knowledge from the discovery of that gene’s structure.2 A genetic variant is a change in the gene sequence. Any change, even one nucleotide, can constitute a variant. Some variants are harmless, but some—termed “mutatio ...
... sequence similarity with any particular gene requires an application of detailed knowledge from the discovery of that gene’s structure.2 A genetic variant is a change in the gene sequence. Any change, even one nucleotide, can constitute a variant. Some variants are harmless, but some—termed “mutatio ...
Assaying … promoter activity
... true breeding experiment. In this experiment, recombinant cells were grown at the non-permissive temperature in the absence of antibiotic selection for approximately 10 generations. Following this period, aliquots from this culture were plated onto selective (LB with 5 mg/L gentamicin) or non-select ...
... true breeding experiment. In this experiment, recombinant cells were grown at the non-permissive temperature in the absence of antibiotic selection for approximately 10 generations. Following this period, aliquots from this culture were plated onto selective (LB with 5 mg/L gentamicin) or non-select ...
Assessment by Molecular Dynamics Simulations of the Structural
... nucleotide sequences different from, but related to and sometimes overlapping, those recognized by the structurally better characterized early growth response protein 1 (EGR1, also known as Zif268, Krox-24, and NGFI-A). The accepted consensus binding sequence for Sp1 is usually defined by the asymme ...
... nucleotide sequences different from, but related to and sometimes overlapping, those recognized by the structurally better characterized early growth response protein 1 (EGR1, also known as Zif268, Krox-24, and NGFI-A). The accepted consensus binding sequence for Sp1 is usually defined by the asymme ...
Is HP1 an RNA detector that functions both in repression and
... with a range of nuclear activities (Kellum, 2003; and references therein). Do RNA sense strands recruit transcriptional activators to sites of active transcription through HP1, while RNA double strands specify targeting of a separate class of HP1 repressing complexes to heterochromatic regions? If s ...
... with a range of nuclear activities (Kellum, 2003; and references therein). Do RNA sense strands recruit transcriptional activators to sites of active transcription through HP1, while RNA double strands specify targeting of a separate class of HP1 repressing complexes to heterochromatic regions? If s ...
Collaborative coupling between polymerase and helicase for
... holoenzyme destabilizes the first few base pairs of the fork thereby increasing the efficiency of helicase unwinding. The model implies that both enzymes are localized at the fork, but does not require a specific interaction between them. The model quantitatively reproduces homologous and heterologo ...
... holoenzyme destabilizes the first few base pairs of the fork thereby increasing the efficiency of helicase unwinding. The model implies that both enzymes are localized at the fork, but does not require a specific interaction between them. The model quantitatively reproduces homologous and heterologo ...
Natiiona Str An l D ate nua 20 NA gy l R 15/ A Da Boa epo /16 tab rd
... I am delighted to present this my first report as the new Chair of the National DNA Database and Fingerprint Strategy Board, having previously chaired the National DNA Database Strategy Board between 2007 and 2011. My thanks go to my predecessor, Chris Sims, whose hard work as Chair of the Board has ...
... I am delighted to present this my first report as the new Chair of the National DNA Database and Fingerprint Strategy Board, having previously chaired the National DNA Database Strategy Board between 2007 and 2011. My thanks go to my predecessor, Chris Sims, whose hard work as Chair of the Board has ...
Transcriptional analysis of the gene for glutamine synthetase II and
... glnII) activity detectable in crude extracts from Streptomyces coelicolor is low compared to the activity of glutamine synthetase I (GSI, encoded by glnA) and to that of GSII from S. viridochromogenes. We have identi®ed and sequenced a 3.9-kb BglII-BamHI fragment carrying the glutamine synthetase II ...
... glnII) activity detectable in crude extracts from Streptomyces coelicolor is low compared to the activity of glutamine synthetase I (GSI, encoded by glnA) and to that of GSII from S. viridochromogenes. We have identi®ed and sequenced a 3.9-kb BglII-BamHI fragment carrying the glutamine synthetase II ...
PDF
... into the correct chromosomal var upstream region. This design enables rapid positive selection for integration into the desired locus as described by Wang et al. [18]. The constructs, cloning strategy, and expression patterns are shown in Figures 1–5. var loci are found in both subtelomeric and inte ...
... into the correct chromosomal var upstream region. This design enables rapid positive selection for integration into the desired locus as described by Wang et al. [18]. The constructs, cloning strategy, and expression patterns are shown in Figures 1–5. var loci are found in both subtelomeric and inte ...
Molecular events during translocation and proofreading extracted
... the polymerase active site prior to the reaction. Second, the enzyme translocates by one nucleotide space along the single-stranded DNA template after a correct incorporation. Third, if an incorrect nucleotide is accidentally incorporated into the primer, the 3 terminus of the primer is switched to ...
... the polymerase active site prior to the reaction. Second, the enzyme translocates by one nucleotide space along the single-stranded DNA template after a correct incorporation. Third, if an incorrect nucleotide is accidentally incorporated into the primer, the 3 terminus of the primer is switched to ...
Genome Biology - Department of Computer Science and
... Upon binding, TFs interact with RNA polymerase II to activate or repress transcription. TFs also recruit chromatin modification enzymes to induce chromatin structure changes, which in turn affect the accessibility of factors to genomic DNA regions [3, 4]. The target genes of a TF change according to ...
... Upon binding, TFs interact with RNA polymerase II to activate or repress transcription. TFs also recruit chromatin modification enzymes to induce chromatin structure changes, which in turn affect the accessibility of factors to genomic DNA regions [3, 4]. The target genes of a TF change according to ...
Cancer epigenetics
Cancer epigenetics is the study of epigenetic modifications to the genome of cancer cells that do not involve a change in the nucleotide sequence. Epigenetic alterations are as important as genetic mutations in a cell’s transformation to cancer, and their manipulation holds great promise for cancer prevention, detection, and therapy. In different types of cancer, a variety of epigenetic mechanisms can be perturbed, such as silencing of tumor suppressor genes and activation of oncogenes by altered CpG island methylation patterns, histone modifications, and dysregulation of DNA binding proteins. Several medications which have epigenetic impact are now used in several of these diseases.