Stage-specific expressions of four different ribonuclease H genes in
... catalytic activity of the protein. Three other Leishmania RNases H share conserved domains with the known type II enzymes (Figure 1). We are not sure about the metal ion requirements of each of these enzymes. Thus, the classification is simply based on sequence ...
... catalytic activity of the protein. Three other Leishmania RNases H share conserved domains with the known type II enzymes (Figure 1). We are not sure about the metal ion requirements of each of these enzymes. Thus, the classification is simply based on sequence ...
Engineered bacteriophage-defence systems in bioprocessing
... genetic content and genomic organization of these seven phages exhibit significant similarities to other members of the λ-superfamily of phages23. Bioinformatic analyses have provided significant insight into the evolution of S. thermophilus phages, revealing that their genomes are molecular mosaics ...
... genetic content and genomic organization of these seven phages exhibit significant similarities to other members of the λ-superfamily of phages23. Bioinformatic analyses have provided significant insight into the evolution of S. thermophilus phages, revealing that their genomes are molecular mosaics ...
345 - Timstar
... genes, the noncoding sequences of genes, or regions in-between genes (the intergenic regions). When an SNP falls within a coding sequence, that alteration may not necessarily change the amino acid sequence of the subsequent protein produced due to degeneracy of the genetic code. On the other hand, S ...
... genes, the noncoding sequences of genes, or regions in-between genes (the intergenic regions). When an SNP falls within a coding sequence, that alteration may not necessarily change the amino acid sequence of the subsequent protein produced due to degeneracy of the genetic code. On the other hand, S ...
Structure and function of the GINS complex, a key component of the
... The effect of the phosphorylation of Sld3 is to generate a binding site from another essential replication factor, Dpb11 (equivalent to TOPBP1 in human cells). The Dpb11 protein possesses four BRCT domains that act in pairs to bind to phosphopeptides [23] and it is the N-terminal pair that is respon ...
... The effect of the phosphorylation of Sld3 is to generate a binding site from another essential replication factor, Dpb11 (equivalent to TOPBP1 in human cells). The Dpb11 protein possesses four BRCT domains that act in pairs to bind to phosphopeptides [23] and it is the N-terminal pair that is respon ...
Simple identification of dominant p53 mutants by
... are unable to function as sequence-specific transcription factors, generally because they contain mutations in the DNA binding domain which reduce the affinity for DNA (3,4). The high level of p53 protein commonly seen in tumours probably reflects the presence of a persistent p53 activating signal a ...
... are unable to function as sequence-specific transcription factors, generally because they contain mutations in the DNA binding domain which reduce the affinity for DNA (3,4). The high level of p53 protein commonly seen in tumours probably reflects the presence of a persistent p53 activating signal a ...
Julio`s MCB - Digital Access to Scholarship at Harvard
... morpholinos and cell sorting analysis in transgenic zebrafish embryos, we show that GATA-1 directly regulates the expression of Mfrn1. Mutagenesis of individual GATA-1 binding cis elements (GBE) demonstrated that at least two of the three GBE within this CRM are functionally required for GATA-mediat ...
... morpholinos and cell sorting analysis in transgenic zebrafish embryos, we show that GATA-1 directly regulates the expression of Mfrn1. Mutagenesis of individual GATA-1 binding cis elements (GBE) demonstrated that at least two of the three GBE within this CRM are functionally required for GATA-mediat ...
Hox Genes: Let`s Work Together
... showed Ubx binding on the promoters of all anterior Hox genes and not posterior Hox genes (Slattery et al., 2011). This observation provides an evidence for direct Ubx-mediated transcriptional regulation of anterior Hox genes. Suppression through Non-coding RNAs Sense and anti-sense long non-coding ...
... showed Ubx binding on the promoters of all anterior Hox genes and not posterior Hox genes (Slattery et al., 2011). This observation provides an evidence for direct Ubx-mediated transcriptional regulation of anterior Hox genes. Suppression through Non-coding RNAs Sense and anti-sense long non-coding ...
Structure-Function Analysis of the Conserved Histone Chaperone
... Chromatin structure is crucial to regulate access to the genome for processes such as transcription, recombination, DNA repair, and DNA replication. Spt6, a key factor involved in regulating chromatin struct ...
... Chromatin structure is crucial to regulate access to the genome for processes such as transcription, recombination, DNA repair, and DNA replication. Spt6, a key factor involved in regulating chromatin struct ...
Short Tandem Repeat Polymorphism and Cancer Risk: Influence of
... in CA repeats between the two methods, including 5 samples with two-repeat difference, 3 with three-repeat difference, 3 with four-repeat difference, and 1 with fiverepeat difference. Of the 12 samples that had the same allelic difference in CA repeats, only 8 had exactly the same number of repeats ...
... in CA repeats between the two methods, including 5 samples with two-repeat difference, 3 with three-repeat difference, 3 with four-repeat difference, and 1 with fiverepeat difference. Of the 12 samples that had the same allelic difference in CA repeats, only 8 had exactly the same number of repeats ...
Centipede Hox genes - Development
... Sets of degenerate primers were used to amplify portions of the various Hox genes. The primers were designed based on the sequences of orthologs from other arthropod species; primer sequences are available upon request. From the clones of the homeobox regions, exact primers were designed for 3′ RACE ...
... Sets of degenerate primers were used to amplify portions of the various Hox genes. The primers were designed based on the sequences of orthologs from other arthropod species; primer sequences are available upon request. From the clones of the homeobox regions, exact primers were designed for 3′ RACE ...
htp Pseudomonas aeruginosa AMANDA SWANEPOEL
... which leads to the formation of biofilms (Zobell, 1943; Costerton et al., 1995). Biofilms have been defined as structured communities of bacterial cells that are enclosed in a self-produced polymeric matrix and adhere to biotic and abiotic surfaces, an interface or each other (Costerton et al., 1995 ...
... which leads to the formation of biofilms (Zobell, 1943; Costerton et al., 1995). Biofilms have been defined as structured communities of bacterial cells that are enclosed in a self-produced polymeric matrix and adhere to biotic and abiotic surfaces, an interface or each other (Costerton et al., 1995 ...
Microbiology
... A series of transposons are described which contain the gusA gene, encoding B-glucuronidase (GUS), expressed from a variety of promoters, both regulated and constitutive. The regulated promoters include the tac promoter which can be induced by IPTG, and nifH promoters which are symbiotically activat ...
... A series of transposons are described which contain the gusA gene, encoding B-glucuronidase (GUS), expressed from a variety of promoters, both regulated and constitutive. The regulated promoters include the tac promoter which can be induced by IPTG, and nifH promoters which are symbiotically activat ...
Article Mitochondrial DNA turnover occurs during preimplantation
... (GV) were isolated from ovaries, unfertilized eggs (UF) were collected 12 h after HCG injection, and fertilized eggs (FE), 2-, 4-, 8-cell and blastocyst stage embryos were collected after successful mating at 24, 36, 48, 60 and 72 h post-HCG (a–g). MtDNA copy number was measured in groups of embryos ...
... (GV) were isolated from ovaries, unfertilized eggs (UF) were collected 12 h after HCG injection, and fertilized eggs (FE), 2-, 4-, 8-cell and blastocyst stage embryos were collected after successful mating at 24, 36, 48, 60 and 72 h post-HCG (a–g). MtDNA copy number was measured in groups of embryos ...
Document
... Mendel’s Law of Segregation A. Law of Segregation states that a pair of factors (alleles) is segregated, or separated, during the formation of gametes (reproductive cells) (1) When two gametes combine during fertilization, the offspring have two factors controlling a specific trait (Gg) ...
... Mendel’s Law of Segregation A. Law of Segregation states that a pair of factors (alleles) is segregated, or separated, during the formation of gametes (reproductive cells) (1) When two gametes combine during fertilization, the offspring have two factors controlling a specific trait (Gg) ...
CBSE XII BIO QS with key(2009-2015)
... a) When and how does placenta develop in human female? b) How is the placenta connected to the embryo? c) Placenta acts as an endocrine gland. Explain. Ans: a) after implantation of the embryo placenta develop in the human female, the chorionic villi, and uterine tissue, become interdigitated with e ...
... a) When and how does placenta develop in human female? b) How is the placenta connected to the embryo? c) Placenta acts as an endocrine gland. Explain. Ans: a) after implantation of the embryo placenta develop in the human female, the chorionic villi, and uterine tissue, become interdigitated with e ...
Probing the evolution of appendage specialization by
... 4–12 h after the onset of heat shock (Fig. 2I) and lower levels of PhScr could be observed up to 1 day later (3/22 embryos). This downregulation provides an explanation for the transformation of Mx2 (which normally expresses high levels of PhScr) to Mxp (which expresses lower levels) in ⬇16% of surv ...
... 4–12 h after the onset of heat shock (Fig. 2I) and lower levels of PhScr could be observed up to 1 day later (3/22 embryos). This downregulation provides an explanation for the transformation of Mx2 (which normally expresses high levels of PhScr) to Mxp (which expresses lower levels) in ⬇16% of surv ...
Evaluation of genomic DNA from paraffin
... of DCM. Cardiomyopathy of Boxers and Doberman Pinschers are called “fatty infiltration-degenerative” type and in many giant, large- and medium-sized breeds DCM can be classified as “attenuated wavy fiber” type. Attenuated wavy fibers are myofibers that are thinner than normal and have wavy appearanc ...
... of DCM. Cardiomyopathy of Boxers and Doberman Pinschers are called “fatty infiltration-degenerative” type and in many giant, large- and medium-sized breeds DCM can be classified as “attenuated wavy fiber” type. Attenuated wavy fibers are myofibers that are thinner than normal and have wavy appearanc ...
Increased sex chromosome expression and epigenetic
... polymerase II, and are enriched for several repressive chromatin marks present on the sex body, including histone H3 dimethylated at lysine 9 (H3K9me2) and CBX1 (chromobox protein homolog 1, previously known as HP1) (Baarends et al., 2007; Greaves et al., 2006; Khalil et al., 2004; Namekawa et al., ...
... polymerase II, and are enriched for several repressive chromatin marks present on the sex body, including histone H3 dimethylated at lysine 9 (H3K9me2) and CBX1 (chromobox protein homolog 1, previously known as HP1) (Baarends et al., 2007; Greaves et al., 2006; Khalil et al., 2004; Namekawa et al., ...
anterior olfactory nucleus (aon) - Dashboard
... The anterior olfactory nucleus (AON) is a substructure of the olfactory areas, located within the rostral portion of the cerebral cortex. The AON is comprised of dorsal (AONd), external (AONe), lateral (AONl), medial (AONm) and posteroventral (AONpv) subdivisions. Rostrally, the AONe surrounds the r ...
... The anterior olfactory nucleus (AON) is a substructure of the olfactory areas, located within the rostral portion of the cerebral cortex. The AON is comprised of dorsal (AONd), external (AONe), lateral (AONl), medial (AONm) and posteroventral (AONpv) subdivisions. Rostrally, the AONe surrounds the r ...
Discovering the Distribution of Palindromic Sequences in the
... titled “mutations.csv”. Three Java programs were written: (Supplementary Data Index 1) reads mutation information from the mutations. csv file and accordingly mutates a .fasta file (Supplementary Data Index 2) finds palindromes and prints the length of each palindrome (length) (Supplementary Data In ...
... titled “mutations.csv”. Three Java programs were written: (Supplementary Data Index 1) reads mutation information from the mutations. csv file and accordingly mutates a .fasta file (Supplementary Data Index 2) finds palindromes and prints the length of each palindrome (length) (Supplementary Data In ...
Genetic Polymorphism of Human CYP2E1
... CYP2E1*2 cDNA, although yielding similar amounts of mRNA, only caused 37% of the protein expression and 36% of the catalytic activity compared with the wild-type cDNA. Complete screening by single-stranded conformation polymorphism of the three populations studied revealed that these variant alleles ...
... CYP2E1*2 cDNA, although yielding similar amounts of mRNA, only caused 37% of the protein expression and 36% of the catalytic activity compared with the wild-type cDNA. Complete screening by single-stranded conformation polymorphism of the three populations studied revealed that these variant alleles ...
Missouri Western State University
... first and second, second and third, all three. • The following slide shows Northern Hemisphere of the 2-spatula burnt pancake graph on a globe. ...
... first and second, second and third, all three. • The following slide shows Northern Hemisphere of the 2-spatula burnt pancake graph on a globe. ...
Lecture-Mic 623-Plasmids-Listeria - Home
... Decimal reduction times of each L. innocua strain were 1.5 to 3 times longer in either heating menstruum than were D-values of the most heat resistant L. monocytogenes strain studied (F5069). L. innocua PFEI retained the plasmid during heating so that, of 300 survivors evaluated, 100% were resistan ...
... Decimal reduction times of each L. innocua strain were 1.5 to 3 times longer in either heating menstruum than were D-values of the most heat resistant L. monocytogenes strain studied (F5069). L. innocua PFEI retained the plasmid during heating so that, of 300 survivors evaluated, 100% were resistan ...
Knockdown of Parhyale Ultrabithorax - IMBB
... developed a strategy to knock down gene function during Parhayle development using injection of siRNAs, a technique effective in several diverse animal taxa. To demonstrate the efficacy of this technique in Parhyale, we chose the Dll gene as a positive control. Dll and its vertebrate homologs, the D ...
... developed a strategy to knock down gene function during Parhayle development using injection of siRNAs, a technique effective in several diverse animal taxa. To demonstrate the efficacy of this technique in Parhyale, we chose the Dll gene as a positive control. Dll and its vertebrate homologs, the D ...
Cancer epigenetics
Cancer epigenetics is the study of epigenetic modifications to the genome of cancer cells that do not involve a change in the nucleotide sequence. Epigenetic alterations are as important as genetic mutations in a cell’s transformation to cancer, and their manipulation holds great promise for cancer prevention, detection, and therapy. In different types of cancer, a variety of epigenetic mechanisms can be perturbed, such as silencing of tumor suppressor genes and activation of oncogenes by altered CpG island methylation patterns, histone modifications, and dysregulation of DNA binding proteins. Several medications which have epigenetic impact are now used in several of these diseases.