Selection for TnlO Tet Repressor Binding to tet Operator
... complete derepression is probably not achieved because Lac repressor is present in such a high amount that it is never fully induced at the IPTG concentration used (10-' M). We have analyzed the selection systemwith an operator constitutivemutation to demonstratethe necessity of functional tet opera ...
... complete derepression is probably not achieved because Lac repressor is present in such a high amount that it is never fully induced at the IPTG concentration used (10-' M). We have analyzed the selection systemwith an operator constitutivemutation to demonstratethe necessity of functional tet opera ...
The universal reagent for genome tailoring
... achieve far more sophisticated genetic alterations than had been possible before. Now we have an enormous set of building blocks from which to select the desired combinations to achieve particular goals (Nagy, 1996). As far as imaginable genetic alterations are concerned, there is not too much left ...
... achieve far more sophisticated genetic alterations than had been possible before. Now we have an enormous set of building blocks from which to select the desired combinations to achieve particular goals (Nagy, 1996). As far as imaginable genetic alterations are concerned, there is not too much left ...
Maneeshi Prasad
... BDNF in CNS • Alternative promoter usage provides differential mRNA stability and subcellular localization • Promoter IV of BDNF has been implicated in forming inhibitory synapses in the cortex • BDNF mRNA with long 3’UTR are localized in dendrites of cortical neurons, while short 3’UTR mRNA is res ...
... BDNF in CNS • Alternative promoter usage provides differential mRNA stability and subcellular localization • Promoter IV of BDNF has been implicated in forming inhibitory synapses in the cortex • BDNF mRNA with long 3’UTR are localized in dendrites of cortical neurons, while short 3’UTR mRNA is res ...
Interaction of nonsense suppressor tRNAs and codon nonsense
... in both a prokaryotic and a eukaryotic host [13]. A temperature-sensitive mutant of E. coli harbors a weak suppressing activity towards a UAG mutation, together with an inability to grow at 43˚C in rich medium. The mutation shows enhanced misreading of UAG/A, but UGA. The mutation gives an increased ...
... in both a prokaryotic and a eukaryotic host [13]. A temperature-sensitive mutant of E. coli harbors a weak suppressing activity towards a UAG mutation, together with an inability to grow at 43˚C in rich medium. The mutation shows enhanced misreading of UAG/A, but UGA. The mutation gives an increased ...
DNA METHODS FOR HLA TYPING A WORKBOOK FOR - ASHI-U
... Hematopoietic stem cells found in the bone marrow differentiate to become blood cells. These cells play important roles in fighting infection (white blood cells or lymphocytes), in transporting oxygen to the tissues (red blood cells), and in causing blood clotting (platelets). Certain types of cance ...
... Hematopoietic stem cells found in the bone marrow differentiate to become blood cells. These cells play important roles in fighting infection (white blood cells or lymphocytes), in transporting oxygen to the tissues (red blood cells), and in causing blood clotting (platelets). Certain types of cance ...
Multiplex in-vitro Detection using SERS
... beneficial in obtaining further enhancement in the form of surface enhanced resonance Raman scattering (SERRS). This occurs when the analyte contains a chromophore close in energy to the exciting radiation. However, a dye label can also be used to achieve this enhancement if the chromophore is not p ...
... beneficial in obtaining further enhancement in the form of surface enhanced resonance Raman scattering (SERRS). This occurs when the analyte contains a chromophore close in energy to the exciting radiation. However, a dye label can also be used to achieve this enhancement if the chromophore is not p ...
Disruption and aberrant expression of HMGA2 as a
... consisting of HMGA2 exons 1–3 fused to intronic sequences from the same gene.8,26 These mRNA variants have been found in all the tumor types described above.8,26–29 These findings, and the fact that there is no obvious functional relationship between the various partner genes, suggest that overexpre ...
... consisting of HMGA2 exons 1–3 fused to intronic sequences from the same gene.8,26 These mRNA variants have been found in all the tumor types described above.8,26–29 These findings, and the fact that there is no obvious functional relationship between the various partner genes, suggest that overexpre ...
Bacteriophage A cloning system for the construction of
... endonucleases at the cloning site, subsequent treatment with phosphatase to ensure high insertion rates would be unnecessary. (iv) DNA inserts can be cloned in a known orientation relative to vector-encoded expression signals. The cloning vector XORF8 possesses a multiple cloning site (MCS) containi ...
... endonucleases at the cloning site, subsequent treatment with phosphatase to ensure high insertion rates would be unnecessary. (iv) DNA inserts can be cloned in a known orientation relative to vector-encoded expression signals. The cloning vector XORF8 possesses a multiple cloning site (MCS) containi ...
Molecular Biology and Applied Genetics
... health institutions. It can also be used in teaching any introductory course on medical Applied Genetics and Molecular Biology and as a reference material. This lecture note is specifically designed for medical laboratory technologists, and includes only those areas of molecular cell biology and App ...
... health institutions. It can also be used in teaching any introductory course on medical Applied Genetics and Molecular Biology and as a reference material. This lecture note is specifically designed for medical laboratory technologists, and includes only those areas of molecular cell biology and App ...
Polygenes and Modifier Genes for Tetracycline and
... A lawn of 24392 grew on all Tet plates except 1.5 p g ml-l. -, no growth. Growth means that the transformants were resistant to Tet at the concen- ...
... A lawn of 24392 grew on all Tet plates except 1.5 p g ml-l. -, no growth. Growth means that the transformants were resistant to Tet at the concen- ...
The evolution of meiotic sex and its alternatives
... damage caused by reactive oxygen species (ROS) [19,20]. Hence, an enzymatic DNA repair machinery already existed in prokaryotes which was taken over by eukaryotes [21]. DNA repair was badly needed in the first eukaryotes because of endogenous production of ROS with the onset of cellular oxygen respi ...
... damage caused by reactive oxygen species (ROS) [19,20]. Hence, an enzymatic DNA repair machinery already existed in prokaryotes which was taken over by eukaryotes [21]. DNA repair was badly needed in the first eukaryotes because of endogenous production of ROS with the onset of cellular oxygen respi ...
Conservation of Brachyury (T) genes in amphioxus and vertebrates
... with a poly(A) tract of 17 adenine residues, preceded by a polyadenylation signal at position 2228 (the complete nucleotide sequence of AmBra-1 is accessible in the EMBL/GenBank/DDBJ databases under accession number X91903). AmBra-2 contains an insert of approximately 1.8 kb, with the potential to e ...
... with a poly(A) tract of 17 adenine residues, preceded by a polyadenylation signal at position 2228 (the complete nucleotide sequence of AmBra-1 is accessible in the EMBL/GenBank/DDBJ databases under accession number X91903). AmBra-2 contains an insert of approximately 1.8 kb, with the potential to e ...
Specialized Transduction by Bacteriophage P22 in Salmonella typhimurium: Genetic and Physical Structure of the Transducing Genomes and the Prophage Attachment Site.
... that carry the proA and proB genes of Salmonella typhimurium. These genes lie immediately adjacent to the prophage attachment site on the bacterial chromosome. By examining DNA heteroduplexes in the electron microscope, we found that DNA molecules from P22pro-I and P22pro-3 each contain a substituti ...
... that carry the proA and proB genes of Salmonella typhimurium. These genes lie immediately adjacent to the prophage attachment site on the bacterial chromosome. By examining DNA heteroduplexes in the electron microscope, we found that DNA molecules from P22pro-I and P22pro-3 each contain a substituti ...
Graph-based Methods for the Design of DNA - TUBdok
... A DNA single strand (ssDNA) is a linear biopolymer consisting of nucleobases (or simply bases) bound to a sugar-phosphate backbone. The alternation of phosphate and sugar gives an orientation to the backbone and to the whole DNA strand. The bases in the strand are usually taken from a phosphate (den ...
... A DNA single strand (ssDNA) is a linear biopolymer consisting of nucleobases (or simply bases) bound to a sugar-phosphate backbone. The alternation of phosphate and sugar gives an orientation to the backbone and to the whole DNA strand. The bases in the strand are usually taken from a phosphate (den ...
Press release
... based on the use of magnetic particles. These particles can specifically bind nucleic acids such as DNA and thus allow the separation of DNA from other contaminants. sbeadex is based on a two-stage binding mechanism, so that water can be used for the final washing step. This avoids having to dry the ...
... based on the use of magnetic particles. These particles can specifically bind nucleic acids such as DNA and thus allow the separation of DNA from other contaminants. sbeadex is based on a two-stage binding mechanism, so that water can be used for the final washing step. This avoids having to dry the ...
Genome-Wide Identification of Allelic Expression in Hypertensive
... crosses between SHR-A3 and SHR/N and between SHR-A3 and SHR-C.9 –11 In each case, blood pressure was measured by tail cuff, a method no longer recommended in the present context12 that can contribute to relatively large within group variances that reduce statistical power and can lead to type II err ...
... crosses between SHR-A3 and SHR/N and between SHR-A3 and SHR-C.9 –11 In each case, blood pressure was measured by tail cuff, a method no longer recommended in the present context12 that can contribute to relatively large within group variances that reduce statistical power and can lead to type II err ...
Genome-Wide Identification of Allelic Expression in Hypertensive Rats
... of sequence variation between SHR-A3 and WKY. Among the 36 genes and ESTs surveyed, 21 have been found to be polymorphic between SHR-A3 and WKY. Genotyping assays were developed and used to determine which of these 21 genes share the same allele in all SHR lines (SHR-A3, -B2, -C, and SHR/N), but con ...
... of sequence variation between SHR-A3 and WKY. Among the 36 genes and ESTs surveyed, 21 have been found to be polymorphic between SHR-A3 and WKY. Genotyping assays were developed and used to determine which of these 21 genes share the same allele in all SHR lines (SHR-A3, -B2, -C, and SHR/N), but con ...
Relation of the Wnt/β-catenin signaling pathway
... called androgen receptor (AR), in the cytosol. After binding, androgen-AR complex is formed and it is transferred to the nucleus. This complex enters the nucleus by passing through the nucleolus membrane and binds DNA to activate the specific genes. If these events occur abnormally, carcinogenic tra ...
... called androgen receptor (AR), in the cytosol. After binding, androgen-AR complex is formed and it is transferred to the nucleus. This complex enters the nucleus by passing through the nucleolus membrane and binds DNA to activate the specific genes. If these events occur abnormally, carcinogenic tra ...
The Rad50 Signature Motif: Essential to ATP Binding and
... protein dimerization upon ATP binding by interaction with the g phosphate group of the ATP molecule bound at the Walker motifs of the other subunit. In addition, in vitro assays indicated that ATP stimulated DNA binding by pfRad50, an observation made previously with yeast Rad50.25 The mechanism for ...
... protein dimerization upon ATP binding by interaction with the g phosphate group of the ATP molecule bound at the Walker motifs of the other subunit. In addition, in vitro assays indicated that ATP stimulated DNA binding by pfRad50, an observation made previously with yeast Rad50.25 The mechanism for ...
Defining characteristics of Tn5 Transposase non
... localization within the global DNA, most likely through a direct transfer mechanism, and that nonspecific DNA binding may play a role in the cis bias manifested by Tn5 transposition. ...
... localization within the global DNA, most likely through a direct transfer mechanism, and that nonspecific DNA binding may play a role in the cis bias manifested by Tn5 transposition. ...
2004-2005
... Medicine, Nagoya, and approximately 30 visiting research fellows. The major areas being pursued are as follows: - descriptive and analytical epidemiology of cancers - primary and secondary prevention of cancer - molecular pathogenesis of gastrointestinal cancers - molecular oncology of lung cancer - ...
... Medicine, Nagoya, and approximately 30 visiting research fellows. The major areas being pursued are as follows: - descriptive and analytical epidemiology of cancers - primary and secondary prevention of cancer - molecular pathogenesis of gastrointestinal cancers - molecular oncology of lung cancer - ...
Cancer epigenetics
Cancer epigenetics is the study of epigenetic modifications to the genome of cancer cells that do not involve a change in the nucleotide sequence. Epigenetic alterations are as important as genetic mutations in a cell’s transformation to cancer, and their manipulation holds great promise for cancer prevention, detection, and therapy. In different types of cancer, a variety of epigenetic mechanisms can be perturbed, such as silencing of tumor suppressor genes and activation of oncogenes by altered CpG island methylation patterns, histone modifications, and dysregulation of DNA binding proteins. Several medications which have epigenetic impact are now used in several of these diseases.