Studying Environmental Influences and Breast Cancer
... The traditional view is that hormones cause breast cancer, except in rare cases where women in high-risk families inherit mutations in genes such as BRCA1 or BRCA2. Recent evidence shows that this view is too narrow. A broader view of breast cancer causation is needed that takes environmental factor ...
... The traditional view is that hormones cause breast cancer, except in rare cases where women in high-risk families inherit mutations in genes such as BRCA1 or BRCA2. Recent evidence shows that this view is too narrow. A broader view of breast cancer causation is needed that takes environmental factor ...
Warren, ST and Ashley, CT: Triplet repeat expansion mutations: The example of fragile X syndrome. Annual Review of Neuroscience 18:77-99 (1995).
... localized this instability to a single 1.0-kb Pst I fragment, pfxa2, that was sequenced and found to contain an unusual CGG trinucleotide repeat of 43 copies. By using the polymerase chain reaction (PCR) and primers flanking the repeat, Kremer et al (1991a) mapped the region of instability in pfxa2 ...
... localized this instability to a single 1.0-kb Pst I fragment, pfxa2, that was sequenced and found to contain an unusual CGG trinucleotide repeat of 43 copies. By using the polymerase chain reaction (PCR) and primers flanking the repeat, Kremer et al (1991a) mapped the region of instability in pfxa2 ...
The Nicotiana tabacum genome encodes two cytoplasmic
... kit from Stratagene, according to the recommendations of the manufacturer. From the partial genomic phage library, 400 O00 plaques of the recombinant bacteriophage were plated at a density of about 50 O00 per plate, transferred onto Hybond N f membrane (Amersham) and screened with the thioredoxin X5 ...
... kit from Stratagene, according to the recommendations of the manufacturer. From the partial genomic phage library, 400 O00 plaques of the recombinant bacteriophage were plated at a density of about 50 O00 per plate, transferred onto Hybond N f membrane (Amersham) and screened with the thioredoxin X5 ...
Document
... and its mutant phenotype we need to study the gene at multiple levels: Genetics- mutant gene- mutant phenotype Now what? Genetics will relate specific mutation to specific phenotype It usually provides No Information about how the protein generates the phenotype For p53 We would like to know The nuc ...
... and its mutant phenotype we need to study the gene at multiple levels: Genetics- mutant gene- mutant phenotype Now what? Genetics will relate specific mutation to specific phenotype It usually provides No Information about how the protein generates the phenotype For p53 We would like to know The nuc ...
SPATULA, a bHLH carpel development gene
... cDNA 3.5 corresponds to predicted gene 44 in the AP2 contig of Terryn et al. (1999) (also called AT4g36930 by the Martinsried Institute for Protein Sequences, see http://www.mips.biochem.mpg.de/proj/thal/). Comparison of cDNA 3.5 and the predicted ORF suggested that it is not full length. Two longer ...
... cDNA 3.5 corresponds to predicted gene 44 in the AP2 contig of Terryn et al. (1999) (also called AT4g36930 by the Martinsried Institute for Protein Sequences, see http://www.mips.biochem.mpg.de/proj/thal/). Comparison of cDNA 3.5 and the predicted ORF suggested that it is not full length. Two longer ...
Imprinting in the endosperm: a possible role in preventing wide
... tissue with an unequal parental genomic contribution, which, like the placenta of mammals, provides reserves to the developing embryo. Interestingly, many aspects of endosperm development, again like the placenta, are regulated by a range of epigenetic mechanisms that are globally termed imprinting. ...
... tissue with an unequal parental genomic contribution, which, like the placenta of mammals, provides reserves to the developing embryo. Interestingly, many aspects of endosperm development, again like the placenta, are regulated by a range of epigenetic mechanisms that are globally termed imprinting. ...
Properties of Mitotic and Meiotic Recombination in the
... (Liang et al., 1998). Saccharomyces cerevisiae strains that lack HR are very sensitive to DNA damaging agents such as X-rays (Resnick and Martin, 1976), and have a high rate of spontaneous chromosome loss (Song and Petes, 2012). HR is essential for cell survival of mammalian cells (Helleday, 2003). ...
... (Liang et al., 1998). Saccharomyces cerevisiae strains that lack HR are very sensitive to DNA damaging agents such as X-rays (Resnick and Martin, 1976), and have a high rate of spontaneous chromosome loss (Song and Petes, 2012). HR is essential for cell survival of mammalian cells (Helleday, 2003). ...
A Physical Gene Map of the Bacteriophage P22 Late
... Therefore SmaI site 2 is located at the other end of the 2.0-kb SmaI fragment at P22 map coordinate 0.958. A packaging fragment extending from pat to SmaI site 1 should be present. However, due to the small size (less than 200 base pairs) and low yield of this fragment, it has not been detected. Xho ...
... Therefore SmaI site 2 is located at the other end of the 2.0-kb SmaI fragment at P22 map coordinate 0.958. A packaging fragment extending from pat to SmaI site 1 should be present. However, due to the small size (less than 200 base pairs) and low yield of this fragment, it has not been detected. Xho ...
Are Restriction Enzymes Recognition Sites Underrepresented in the
... both enzymes, the effective recognition of the host restriction site sequence and the foreign restriction site sequence by the methyltransferase enzyme and restriction enzymes, respectively. The net cellular concentration of the active S-adenosyl methionine is important factor that strongly influenc ...
... both enzymes, the effective recognition of the host restriction site sequence and the foreign restriction site sequence by the methyltransferase enzyme and restriction enzymes, respectively. The net cellular concentration of the active S-adenosyl methionine is important factor that strongly influenc ...
MEF2 activity during embryogenesis - Development
... et al., 1989; Cserjesi and Olson, 1991). Whereas a single MEF2-binding site is transcriptionally inactive, when the ...
... et al., 1989; Cserjesi and Olson, 1991). Whereas a single MEF2-binding site is transcriptionally inactive, when the ...
by Vanessa Di Gioacchino A thesis submitted to the Department of Biology
... Goα47A is a candidate gene in a putative haloperidol-affected pathway that is highly expressed in the nervous system. The gene encodes nine transcripts and two isoforms, which are 98% identical and differ only in the N-terminal region. However, the individual roles for each of the two different prot ...
... Goα47A is a candidate gene in a putative haloperidol-affected pathway that is highly expressed in the nervous system. The gene encodes nine transcripts and two isoforms, which are 98% identical and differ only in the N-terminal region. However, the individual roles for each of the two different prot ...
Genome-wide characteristics of sequence coverage by next
... 2003 - Essential Completion = 30, 000 – 40,000 genes ?? = 24, 195 genes !!!??? ...
... 2003 - Essential Completion = 30, 000 – 40,000 genes ?? = 24, 195 genes !!!??? ...
Visualizing expression patterns of Shh and Foxf1 genes
... 9]. Homozygous Shh mutant mouse embryos are characterized by a number of development defects; especially in the respiratory tract that include failure of the trachea to develop as a separate structure from the oesophagus [8, 10]. In Adriamycin treated rats, the level of Shh protein expression is ver ...
... 9]. Homozygous Shh mutant mouse embryos are characterized by a number of development defects; especially in the respiratory tract that include failure of the trachea to develop as a separate structure from the oesophagus [8, 10]. In Adriamycin treated rats, the level of Shh protein expression is ver ...
Gene replacement with the human BRCA1 locus
... BRCA1 are very dicult to obtain and doubly mutant mouse cells grow poorly due to high rates of chromosomal loss associated with defective G2 checkpoint control (Xu et al., 1999). Mouse cells lacking functional Brca1 are also more sensitive to ionizing radiation and oxidative stress (Shen et al., 19 ...
... BRCA1 are very dicult to obtain and doubly mutant mouse cells grow poorly due to high rates of chromosomal loss associated with defective G2 checkpoint control (Xu et al., 1999). Mouse cells lacking functional Brca1 are also more sensitive to ionizing radiation and oxidative stress (Shen et al., 19 ...
ACTIN-RELATED PROTEIN6 Regulates Female Meiosis By
... SWR1 complex are essential for viability; the remainder, including the SWR1 ATPase, enhance complex function (Mizuguchi et al., 2004). Homologs of all yeast and 11 human SWR1 subunits have been identified in Arabidopsis thaliana (March-Díaz and Reyes, 2009; Meagher et al., 2009), indicating that Arab ...
... SWR1 complex are essential for viability; the remainder, including the SWR1 ATPase, enhance complex function (Mizuguchi et al., 2004). Homologs of all yeast and 11 human SWR1 subunits have been identified in Arabidopsis thaliana (March-Díaz and Reyes, 2009; Meagher et al., 2009), indicating that Arab ...
Histological classification and molecular genetics of meningiomas
... risk is increased mitotic activity—ie, four or more mitoses per ten high-power fields (figure 2). Nevertheless, in the absence of increased mitotic activity, other histological features are associated with the likelihood of recurrence and therefore have grading implications. With the WHO 2000 definitio ...
... risk is increased mitotic activity—ie, four or more mitoses per ten high-power fields (figure 2). Nevertheless, in the absence of increased mitotic activity, other histological features are associated with the likelihood of recurrence and therefore have grading implications. With the WHO 2000 definitio ...
Screening for Colorectal Cancer - US Preventive Services Task Force
... The evidence is convincing that screening for colorectal cancer with fecal occult blood testing, sigmoidoscopy, or colonoscopy detects early-stage cancer and adenomatous polyps. Although colonoscopy is considered to be the reference standard against which the sensitivity of other colorectal cancer s ...
... The evidence is convincing that screening for colorectal cancer with fecal occult blood testing, sigmoidoscopy, or colonoscopy detects early-stage cancer and adenomatous polyps. Although colonoscopy is considered to be the reference standard against which the sensitivity of other colorectal cancer s ...
factor occupancy and gene expression Effects of
... Chromatin state may also play a role either in increasing TF occupancy at variants bound by multiple TFs, or in maintaining a state established by pioneer factors. In support of this hypothesis, the DNA near TF hubs had increased sensitivity to DNase I when compared with regions bound by a single fa ...
... Chromatin state may also play a role either in increasing TF occupancy at variants bound by multiple TFs, or in maintaining a state established by pioneer factors. In support of this hypothesis, the DNA near TF hubs had increased sensitivity to DNase I when compared with regions bound by a single fa ...
factor occupancy and gene expression Effects of sequence variation
... Chromatin state may also play a role either in increasing TF occupancy at variants bound by multiple TFs, or in maintaining a state established by pioneer factors. In support of this hypothesis, the DNA near TF hubs had increased sensitivity to DNase I when compared with regions bound by a single fa ...
... Chromatin state may also play a role either in increasing TF occupancy at variants bound by multiple TFs, or in maintaining a state established by pioneer factors. In support of this hypothesis, the DNA near TF hubs had increased sensitivity to DNase I when compared with regions bound by a single fa ...
factor occupancy and gene expression Effects of sequence variation
... Chromatin state may also play a role either in increasing TF occupancy at variants bound by multiple TFs, or in maintaining a state established by pioneer factors. In support of this hypothesis, the DNA near TF hubs had increased sensitivity to DNase I when compared with regions bound by a single fa ...
... Chromatin state may also play a role either in increasing TF occupancy at variants bound by multiple TFs, or in maintaining a state established by pioneer factors. In support of this hypothesis, the DNA near TF hubs had increased sensitivity to DNase I when compared with regions bound by a single fa ...
Gene Section MLL (myeloid/lymphoid or
... Transcriptional regulatory factor, maintenance of Hox gene expression. ...
... Transcriptional regulatory factor, maintenance of Hox gene expression. ...
Primer on Molecular Biology
... • Along with eye color and sex, other genes, such as body color and wing size, had a higher probability of being co-inherited by the offspring genes are linked. • Morgan hypothesized that the closer the genes were located on the a chromosome, the more often the genes are co-inherited. ...
... • Along with eye color and sex, other genes, such as body color and wing size, had a higher probability of being co-inherited by the offspring genes are linked. • Morgan hypothesized that the closer the genes were located on the a chromosome, the more often the genes are co-inherited. ...
Cancer epigenetics
Cancer epigenetics is the study of epigenetic modifications to the genome of cancer cells that do not involve a change in the nucleotide sequence. Epigenetic alterations are as important as genetic mutations in a cell’s transformation to cancer, and their manipulation holds great promise for cancer prevention, detection, and therapy. In different types of cancer, a variety of epigenetic mechanisms can be perturbed, such as silencing of tumor suppressor genes and activation of oncogenes by altered CpG island methylation patterns, histone modifications, and dysregulation of DNA binding proteins. Several medications which have epigenetic impact are now used in several of these diseases.