Nucleotide Sequence Preservation of Human
... tions? We have examined mtDNA3 isolated from neoplastic cells of patients with leukemia. We chose to study the mtDNA of human leukemic cells for 3 reasons: (a) mtDNA is well charac terized; its nucleotide sequence is known in entirety (3), and a great deal is known about between-individual nucleotid ...
... tions? We have examined mtDNA3 isolated from neoplastic cells of patients with leukemia. We chose to study the mtDNA of human leukemic cells for 3 reasons: (a) mtDNA is well charac terized; its nucleotide sequence is known in entirety (3), and a great deal is known about between-individual nucleotid ...
DNA sequence of the rat growth hormone gene: location of the 5
... All d i g e s t i o n s v/ere done with enzymes purchased fran e i t h e r Mew England Biolabs, rtethesda Pesearch Laboratories, o r Poehringer f'annheim. Digestions were usually done with a s u b s t a n t i a l excess of enzyme and approximately in accordance with t h e conditions provided by t h ...
... All d i g e s t i o n s v/ere done with enzymes purchased fran e i t h e r Mew England Biolabs, rtethesda Pesearch Laboratories, o r Poehringer f'annheim. Digestions were usually done with a s u b s t a n t i a l excess of enzyme and approximately in accordance with t h e conditions provided by t h ...
Altering gene expression by aminocoumarins: the role of DNA
... twofold repressed in novobiocin treated bacteria) via light grey (unchanged) to dark orange (at least twofold induced in novobiocin treated cells). Strain HG001 was grown to exponential phase (OD600 = 0.6) and treated with and without novobiocin (0.5 mg/L) for one hour. ...
... twofold repressed in novobiocin treated bacteria) via light grey (unchanged) to dark orange (at least twofold induced in novobiocin treated cells). Strain HG001 was grown to exponential phase (OD600 = 0.6) and treated with and without novobiocin (0.5 mg/L) for one hour. ...
PowerPoint-Präsentation
... Figure S6. Analyses of the primary RpoN regulon in P. aeruginosa A The PseudoCAP annotation (Winsor et al, 2005) was used to categorize the members of the primary RpoN regulon and the enrichment of specific gene classes is displayed. Strong and moderate over-represented classes are highlighted in d ...
... Figure S6. Analyses of the primary RpoN regulon in P. aeruginosa A The PseudoCAP annotation (Winsor et al, 2005) was used to categorize the members of the primary RpoN regulon and the enrichment of specific gene classes is displayed. Strong and moderate over-represented classes are highlighted in d ...
Resolvase OsGEN1 Mediates DNA Repair by
... and Sancar, 2003; Whitby et al., 2003; Taylor and McGowan, 2008). SLX1 is the catalytic subunit of the SLX1-SLX4 heterodimer endonuclease complex and belongs to the GIY-YIG nuclease family (Fricke and Brill, 2003; Dunin-Horkawicz et al., 2006). The SLX1SLX4 complex prefers to cut 59 flap structures i ...
... and Sancar, 2003; Whitby et al., 2003; Taylor and McGowan, 2008). SLX1 is the catalytic subunit of the SLX1-SLX4 heterodimer endonuclease complex and belongs to the GIY-YIG nuclease family (Fricke and Brill, 2003; Dunin-Horkawicz et al., 2006). The SLX1SLX4 complex prefers to cut 59 flap structures i ...
Prevention of DNA Rereplication Through a Meiotic Recombination
... kinase inhibitor Sic1 during meiosis can trigger extra rounds of DNA replication. When programmed DNA double-strand breaks are generated but not repaired due to absence of DMC1, a pathway involving the checkpoint gene RAD17 prevents this DNA rereplication. Further genetic analysis has now reveale ...
... kinase inhibitor Sic1 during meiosis can trigger extra rounds of DNA replication. When programmed DNA double-strand breaks are generated but not repaired due to absence of DMC1, a pathway involving the checkpoint gene RAD17 prevents this DNA rereplication. Further genetic analysis has now reveale ...
A DNA Polymerase ε Mutant That Specifically Causes 1
... 1089 is located 83 amino acids downstream from the last of the polymerase domains (Figure 1A) in a region that has been proposed to be involved in subunit interactions (Kesti et al. 1993). To demonstrate that the C1089Y substitution is the only alteration in POLε required for the mutator phenotype, ...
... 1089 is located 83 amino acids downstream from the last of the polymerase domains (Figure 1A) in a region that has been proposed to be involved in subunit interactions (Kesti et al. 1993). To demonstrate that the C1089Y substitution is the only alteration in POLε required for the mutator phenotype, ...
Transcriptional control of glial cell development in Drosophila
... 100 kb in mammals) of the exons or within the introns of the genes they control, and contain clustered binding sites for multiple transcriptional activators and repressors. Modules often work independently of one another to direct composite patterns of cell-specific gene expression when linked withi ...
... 100 kb in mammals) of the exons or within the introns of the genes they control, and contain clustered binding sites for multiple transcriptional activators and repressors. Modules often work independently of one another to direct composite patterns of cell-specific gene expression when linked withi ...
Chpt9_Transposition.doc
... occupy approximately half the human genome and a substantially greater fraction of some plant genomes! These movable elements are ubiquitous in the biosphere, and are highly successful in propagating themselves. We now realize that some transposable elements are also viruses, for instance, some retr ...
... occupy approximately half the human genome and a substantially greater fraction of some plant genomes! These movable elements are ubiquitous in the biosphere, and are highly successful in propagating themselves. We now realize that some transposable elements are also viruses, for instance, some retr ...
HIGH FREQUENCY GENE TARGETING USING INSERTIONAL
... recombination was 1 in 46 (2.2%). This frequency is significantly higher than those reported by two other groups using isogenic DNA sequence replacement constructs (11,12). All three groups were targeting the same region (at and around exon 10 of the Cftr gene), although the replacement targeting co ...
... recombination was 1 in 46 (2.2%). This frequency is significantly higher than those reported by two other groups using isogenic DNA sequence replacement constructs (11,12). All three groups were targeting the same region (at and around exon 10 of the Cftr gene), although the replacement targeting co ...
One of the crucial proteins to influence type 2 diabetes
... IVS5-13insC and the presence of type 2 diabetes. To better understand, some studies that include GWAS datasets will help determine whether the IVS5-13insC shows a consistent association with type 2 diabetes. However, the direct mechanism by which this variant affects mRNA expression or amino acid se ...
... IVS5-13insC and the presence of type 2 diabetes. To better understand, some studies that include GWAS datasets will help determine whether the IVS5-13insC shows a consistent association with type 2 diabetes. However, the direct mechanism by which this variant affects mRNA expression or amino acid se ...
Transition Bias
... I often say that when you can measure what you are speaking about, and express it in numbers, you know something about it; but when you cannot measure it, when you cannot express it in numbers, your knowledge is of a meagre and unsatisfactory kind; it may be the beginning of knowledge, but you have ...
... I often say that when you can measure what you are speaking about, and express it in numbers, you know something about it; but when you cannot measure it, when you cannot express it in numbers, your knowledge is of a meagre and unsatisfactory kind; it may be the beginning of knowledge, but you have ...
PDF
... transposon-based vectors remains the most suitable gene-delivery system for producing transgenic insects. The most common in current use are vectors based on P- element piggyBac, mariner, Minos, hobo and Hermes [4,7]. Such standard nonautonomous vectors contain target DNA surrounded by transposon se ...
... transposon-based vectors remains the most suitable gene-delivery system for producing transgenic insects. The most common in current use are vectors based on P- element piggyBac, mariner, Minos, hobo and Hermes [4,7]. Such standard nonautonomous vectors contain target DNA surrounded by transposon se ...
Gene Section USF1 (upstream transcription factor 1) Atlas of Genetics and Cytogenetics
... telomerase reverse transcriptase TERT (McMurray and McCance, 2003; Chang et al., 2005). Second, USF1 is involved in cell cycle control (Cogswell et al., 1995) and overexpression of USF1 slows G2/M transition in thyrocytes and thyroid carcinoma cells (Jung et al., 2007). Third, USF1 overexpression le ...
... telomerase reverse transcriptase TERT (McMurray and McCance, 2003; Chang et al., 2005). Second, USF1 is involved in cell cycle control (Cogswell et al., 1995) and overexpression of USF1 slows G2/M transition in thyrocytes and thyroid carcinoma cells (Jung et al., 2007). Third, USF1 overexpression le ...
RECOMBINANT DNA TECHNOLOGY AND BIOTECHNOLOGY
... system will be rendered ineffective. In this case, the phage’s DNA will already be methylated at appropriate sequence and will be recognized as self. Structural and biochemical studies have indicated that for the type II R/M system, the methylase recognizes and methylates one strand of DNA duplex, w ...
... system will be rendered ineffective. In this case, the phage’s DNA will already be methylated at appropriate sequence and will be recognized as self. Structural and biochemical studies have indicated that for the type II R/M system, the methylase recognizes and methylates one strand of DNA duplex, w ...
Bruce Wallace Biotechnology Lab Program Student Guide 5th
... molecular biology have been modified through genetic engineering to facilitate gene cloning and protein production (gene expression) in bacteria. Antibiotic resistant genes have been engineered into these plasmids and function as selectable markers—that is to say, these genes allow us to select betw ...
... molecular biology have been modified through genetic engineering to facilitate gene cloning and protein production (gene expression) in bacteria. Antibiotic resistant genes have been engineered into these plasmids and function as selectable markers—that is to say, these genes allow us to select betw ...
Red Line Walk-through
... translated into amino acid sequences. UniGene: A database of transcript data, “each UniGene entry is a set of transcript sequences that appear to come from the same transcription locus (gene or expressed pseudogene), together with information on protein similarities, gene expression, cDNA clone reag ...
... translated into amino acid sequences. UniGene: A database of transcript data, “each UniGene entry is a set of transcript sequences that appear to come from the same transcription locus (gene or expressed pseudogene), together with information on protein similarities, gene expression, cDNA clone reag ...
Molecular Cloning of Streptococcus bovis Lactose
... has a polar effect on the transacetylase gene. Alteration of the latter sequence does not reduce expression of the other lac genes. These data indicate that the lac genes are under operon control with the direction of transcription running from the P-D-galactosidase locus to the transacetylase gene. ...
... has a polar effect on the transacetylase gene. Alteration of the latter sequence does not reduce expression of the other lac genes. These data indicate that the lac genes are under operon control with the direction of transcription running from the P-D-galactosidase locus to the transacetylase gene. ...
Active repressors
... Housekeeping genes seem to be arranged in clusters that replicate early, whereas many tissuespecific genes replicate late in most tissues, but become early-replicating in cells in which they are expressed ...
... Housekeeping genes seem to be arranged in clusters that replicate early, whereas many tissuespecific genes replicate late in most tissues, but become early-replicating in cells in which they are expressed ...
Mutations: The Effect On Phenotype
... how and why the ability to taste certain compounds differs from person to person. In the 1930’s scientists discovered that some people can taste a bitter compound known as PTC (phenylthiocarbamide), while others cannot. In order to taste something, a receptor on the tongue bindsi a specific chemical ...
... how and why the ability to taste certain compounds differs from person to person. In the 1930’s scientists discovered that some people can taste a bitter compound known as PTC (phenylthiocarbamide), while others cannot. In order to taste something, a receptor on the tongue bindsi a specific chemical ...
Methods of screening for bioactive agents using cells transformed
... draWbacks. First, the presence of viral promoters at the 5‘ long terminal repeats (LTR) may result in mobiliZation or rescue of an integrated provirus by endogenous retroviruses or upon infection With retroviral vectors that express viral ...
... draWbacks. First, the presence of viral promoters at the 5‘ long terminal repeats (LTR) may result in mobiliZation or rescue of an integrated provirus by endogenous retroviruses or upon infection With retroviral vectors that express viral ...
Sequence analysis of three mitochondrial DNA molecules reveals
... no. NC002653) and Yarrowia lipolytica (9) have mtDNA sizes of 27.7, 40.2 and 47.9 kb, respectively. Even in the genus Brettanomyces/Dekkera, mitochondrial genome sizes have been shown to vary from ~28 to ~101 kb (7,10). The mitochondrial genomes of some ascomycetous yeasts have been characterised by ...
... no. NC002653) and Yarrowia lipolytica (9) have mtDNA sizes of 27.7, 40.2 and 47.9 kb, respectively. Even in the genus Brettanomyces/Dekkera, mitochondrial genome sizes have been shown to vary from ~28 to ~101 kb (7,10). The mitochondrial genomes of some ascomycetous yeasts have been characterised by ...
The orthopoxvirus 68k ankyrin-like protein is essential for DNA
... with 1⫻ SDS lysis buffer were cleared with Qiashredder columns (Qiagen), and for NET-N lysates the cell debris was removed by centrifugation. Lysates were separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDSPAGE) and transferred to polyvinylidene difluoride (PVDF) membranes. A ...
... with 1⫻ SDS lysis buffer were cleared with Qiashredder columns (Qiagen), and for NET-N lysates the cell debris was removed by centrifugation. Lysates were separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDSPAGE) and transferred to polyvinylidene difluoride (PVDF) membranes. A ...
The evolution of large DNA viruses: combining genomic information
... capture event by a poxviral ancestor before primate and rodent orders diverged, although a larger sample of taxa are required to confirm this [20]. Finally, a recent case of host gene capture was observed in a mammalian 2b-1,6-Nacetylglucosaminyltransferase-mucin type protein and its only known vira ...
... capture event by a poxviral ancestor before primate and rodent orders diverged, although a larger sample of taxa are required to confirm this [20]. Finally, a recent case of host gene capture was observed in a mammalian 2b-1,6-Nacetylglucosaminyltransferase-mucin type protein and its only known vira ...
Primary transcript
A primary transcript is the single-stranded ribonucleic acid (RNA) product synthesized by transcription of DNA, and processed to yield various mature RNA products such as mRNAs, tRNAs, and rRNAs. The primary transcripts designated to be mRNAs are modified in preparation for translation. For example, a precursor messenger RNA (pre-mRNA) is a type of primary transcript that becomes a messenger RNA (mRNA) after processing.There are several steps contributing to the production of primary transcripts. All these steps involve a series of interactions to initiate and complete the transcription of DNA in the nucleus of eukaryotes. Certain factors play key roles in the activation and inhibition of transcription, where they regulate primary transcript production. Transcription produces primary transcripts that are further modified by several processes. These processes include the 5' cap, 3'-polyadenylation, and alternative splicing. In particular, alternative splicing directly contributes to the diversity of mRNA found in cells. The modifications of primary transcripts have been further studied in research seeking greater knowledge of the role and significance of these transcripts. Experimental studies based on molecular changes to primary transcripts the processes before and after transcription have led to greater understanding of diseases involving primary transcripts.