The Non-LTR Retrotransposon Rex3 from the Fish Xiphophorus is
... crossings with another Xiphophorus species having neither Tu nor R allows expression of the oncogenic potential of Tu in some hybrids. Molecular cloning revealed that the Tu locus includes the Xmrk oncogene, which encodes a novel receptor tyrosine kinase (Wittbrodt et al. 1989). The Tu locus emerged ...
... crossings with another Xiphophorus species having neither Tu nor R allows expression of the oncogenic potential of Tu in some hybrids. Molecular cloning revealed that the Tu locus includes the Xmrk oncogene, which encodes a novel receptor tyrosine kinase (Wittbrodt et al. 1989). The Tu locus emerged ...
p53 powerpoint
... into the minor groove of the DNA (shown in blue and green), forming a strong stabilizing interaction. When mutated to another amino acid, this interaction is lost. Other key sites of mutation are shown in pink, including arginine residues 175, 249, 273 and 282, and ...
... into the minor groove of the DNA (shown in blue and green), forming a strong stabilizing interaction. When mutated to another amino acid, this interaction is lost. Other key sites of mutation are shown in pink, including arginine residues 175, 249, 273 and 282, and ...
Host-range expansion of Spodoptera exigua multiple
... Lawler, 1981; Horton & Burand, 1993). The NCs are transported to the nucleus in a cytoskeleton-dependent manner and enter the nucleus through nuclear pores (Ohkawa et al., 2002), after which transcription and replication can occur. Host-range data are often lacking when a novel virus species is desc ...
... Lawler, 1981; Horton & Burand, 1993). The NCs are transported to the nucleus in a cytoskeleton-dependent manner and enter the nucleus through nuclear pores (Ohkawa et al., 2002), after which transcription and replication can occur. Host-range data are often lacking when a novel virus species is desc ...
Vectors for expression and modification of cDNA sequences in
... genes by a number of different groups. However, most of the commonly available sources of this promoter require extensive sub-cloning and modification before they can be used as effective expression vectors. We report the construction of two plasmids that allow direct cloning and subsequent expressi ...
... genes by a number of different groups. However, most of the commonly available sources of this promoter require extensive sub-cloning and modification before they can be used as effective expression vectors. We report the construction of two plasmids that allow direct cloning and subsequent expressi ...
Initial Stages in Creating a lacI Knockout in Escherichia coli C29
... Primer design and subsequent amplification of the promoter-less kanamycin resistance gene from the pACYC177 plasmid, preceded the second electroporation of the C29 cells harboring pKD46. The PCR product was thought to be designed such that the flanking ends were homologous to the termini of the lacI ...
... Primer design and subsequent amplification of the promoter-less kanamycin resistance gene from the pACYC177 plasmid, preceded the second electroporation of the C29 cells harboring pKD46. The PCR product was thought to be designed such that the flanking ends were homologous to the termini of the lacI ...
DNA Methylation Maintains Allele-specific KIR Gene Expression in
... To investigate expression of other KIR alleles, we further examined donor K, who is heterozygous at the 3DL1, 3DL2, and 2DL4 loci. After amplification with gene-specific primers, bulk RT-PCR products were sequenced. The presence of one or two signals at polymorphic sites indicated monoallelic and b ...
... To investigate expression of other KIR alleles, we further examined donor K, who is heterozygous at the 3DL1, 3DL2, and 2DL4 loci. After amplification with gene-specific primers, bulk RT-PCR products were sequenced. The presence of one or two signals at polymorphic sites indicated monoallelic and b ...
Mouse genetics provides insight into folliculogenesis, fertilization
... cells just prior to primordial follicle formation (Pepling and Spradling, 2001). In the neonatal ovary, each surviving dictyate oocyte is enclosed in a layer of ¯attened pre-granulosa cells which, in turn, are surrounded by a basal lamina to form primordial follicles (Hirsh®eld, 1991). The primordia ...
... cells just prior to primordial follicle formation (Pepling and Spradling, 2001). In the neonatal ovary, each surviving dictyate oocyte is enclosed in a layer of ¯attened pre-granulosa cells which, in turn, are surrounded by a basal lamina to form primordial follicles (Hirsh®eld, 1991). The primordia ...
Duplication of an approximately 1.5 Mb DNA segment
... Chromosome 5q21-22 region in involved in genetic changes of other types of cancer as well. Several studies have demonstrated loss of one allele at chromosome 5q21-22 bands and mutation of the APC gene in the germ line of patients with familial adenomatous polyposis coli and also in sporadic colon ca ...
... Chromosome 5q21-22 region in involved in genetic changes of other types of cancer as well. Several studies have demonstrated loss of one allele at chromosome 5q21-22 bands and mutation of the APC gene in the germ line of patients with familial adenomatous polyposis coli and also in sporadic colon ca ...
lac
... host cells, called its host range. • Viruses identify host cells by a “lock-and-key” fit between proteins on the outside of virus and specific receptor molecules on the host’s surface. • Some viruses infect several species, while others infect only a single species. • Most viruses of eukaryotes atta ...
... host cells, called its host range. • Viruses identify host cells by a “lock-and-key” fit between proteins on the outside of virus and specific receptor molecules on the host’s surface. • Some viruses infect several species, while others infect only a single species. • Most viruses of eukaryotes atta ...
HW4_final
... This is an open research question; you are requested only to write your research plan (as described below) and not conduct the research!!! As we discussed in class it has been proposed that long non coding RNAs (lncRNAs) can bind Transcription Factors (which usually bind double-stranded DNA) and com ...
... This is an open research question; you are requested only to write your research plan (as described below) and not conduct the research!!! As we discussed in class it has been proposed that long non coding RNAs (lncRNAs) can bind Transcription Factors (which usually bind double-stranded DNA) and com ...
Transition bias and substitution models
... I often say that when you can measure what you are speaking about, and express it in numbers, you know something about it; but when you cannot measure it, when you cannot express it in numbers, your knowledge is of a meagre and unsatisfactory kind; it may be the beginning of knowledge, but you have ...
... I often say that when you can measure what you are speaking about, and express it in numbers, you know something about it; but when you cannot measure it, when you cannot express it in numbers, your knowledge is of a meagre and unsatisfactory kind; it may be the beginning of knowledge, but you have ...
A forkhead-domain gene is mutated in a severe speech and
... vitro functional assays19, indicate that inactivation or loss of the forkhead domain is a general mechanism by which mutation of FOX genes can lead to human disease states. Investigations of forkhead-domain mutations associated with autosomal dominant traits suggests that the resulting disorders ar ...
... vitro functional assays19, indicate that inactivation or loss of the forkhead domain is a general mechanism by which mutation of FOX genes can lead to human disease states. Investigations of forkhead-domain mutations associated with autosomal dominant traits suggests that the resulting disorders ar ...
Highly precise and developmentally programmed genome
... expression but is destroyed at each sexual cycle, while the germline micronucleus (MIC) undergoes meiosis and transmits its genome to the zygotic nucleus. New MICs and MACs of sexual progeny differentiate from copies of the zygotic nucleus and extensive genome rearrangements take place in the new MA ...
... expression but is destroyed at each sexual cycle, while the germline micronucleus (MIC) undergoes meiosis and transmits its genome to the zygotic nucleus. New MICs and MACs of sexual progeny differentiate from copies of the zygotic nucleus and extensive genome rearrangements take place in the new MA ...
A plant dialect of the histone language
... considerable research interest because it is associated with transcriptional regulation and methyl transferases contain well conserved domains among different organisms. In contrast to other modifications, no histone demethylases have thus far been identified, although theoretical studies recently p ...
... considerable research interest because it is associated with transcriptional regulation and methyl transferases contain well conserved domains among different organisms. In contrast to other modifications, no histone demethylases have thus far been identified, although theoretical studies recently p ...
Identification of functional domains in Arabidopsis thaliana mRNA
... competition for limited translation initiation factors, primarily eIF4E (1). Cap-independent translation mechanisms such as re-initiation and internal initiation generally lack the efficiency associated with cap-dependent translation (1). Since capping is co-transcriptional and occurs in the nucleus ( ...
... competition for limited translation initiation factors, primarily eIF4E (1). Cap-independent translation mechanisms such as re-initiation and internal initiation generally lack the efficiency associated with cap-dependent translation (1). Since capping is co-transcriptional and occurs in the nucleus ( ...
A DEAD Box RNA Helicase Is Essential for mRNA Export and
... genes and renders plants more sensitive to chilling and freezing stresses. Ectopic expression of CBF3 rescues the chilling and freezing sensitive phenotypes of los4-1 mutant plants, suggesting an important role of the CBF regulon in not only freezing tolerance but also chilling tolerance. LOS4 encod ...
... genes and renders plants more sensitive to chilling and freezing stresses. Ectopic expression of CBF3 rescues the chilling and freezing sensitive phenotypes of los4-1 mutant plants, suggesting an important role of the CBF regulon in not only freezing tolerance but also chilling tolerance. LOS4 encod ...
Activity Name - Science4Inquiry.com
... Something Old, Something New… You are a Molecular Biologist who is studying the process of DNA replication. Your task is to build a model that represents this process. Next you will investigate where DNA replication took place in the human body and explain why it occurred. This activity is divided u ...
... Something Old, Something New… You are a Molecular Biologist who is studying the process of DNA replication. Your task is to build a model that represents this process. Next you will investigate where DNA replication took place in the human body and explain why it occurred. This activity is divided u ...
The Spectrum and Frequency of Self
... primer pair a+d amplifies the entire transposon plus the adjacent bz sequences in long-range PCR. The bz-s derivatives were then grouped into six categories according to the PCR results (Figure 2B). The parental bz-m39(Ac) allele produces junction PCR products with the a+b and c+d primer pairs and tw ...
... primer pair a+d amplifies the entire transposon plus the adjacent bz sequences in long-range PCR. The bz-s derivatives were then grouped into six categories according to the PCR results (Figure 2B). The parental bz-m39(Ac) allele produces junction PCR products with the a+b and c+d primer pairs and tw ...
From Molecular Systems to Simple Cells: a - TBI
... In any living organism, phenotype refers to the physical, organizational and behavioral expression during its lifetime. Genotype refers to a heritable repository of information that instructs the production of molecules whose interactions, in conjunction with the environment, generate and maintain t ...
... In any living organism, phenotype refers to the physical, organizational and behavioral expression during its lifetime. Genotype refers to a heritable repository of information that instructs the production of molecules whose interactions, in conjunction with the environment, generate and maintain t ...
A xylem-specific cellulose synthase gene from aspen (Populus
... plants were bent to create tension stress for various time intervals (from 4 to 40 h). Surprisingly, tension stress rapidly induced phloem-speci®c GUS expression (Figure 5), but did not cause an apparent change in GUS expression in the developing xylem (Figure 5b). Thus under a normal developmental ...
... plants were bent to create tension stress for various time intervals (from 4 to 40 h). Surprisingly, tension stress rapidly induced phloem-speci®c GUS expression (Figure 5), but did not cause an apparent change in GUS expression in the developing xylem (Figure 5b). Thus under a normal developmental ...
Molecular cloning and functional characterisation of a glucose
... We have cloned the first glucose transporter CaHGT1 (Candida albicans high-affinity glucose transporter) of a pathogenic yeast, Candida albicans. The DNA sequence (GenBank accession number Y16834) analysis revealed an ORF encoding a novel protein of 545 amino acids with a predicted molecular mass of ...
... We have cloned the first glucose transporter CaHGT1 (Candida albicans high-affinity glucose transporter) of a pathogenic yeast, Candida albicans. The DNA sequence (GenBank accession number Y16834) analysis revealed an ORF encoding a novel protein of 545 amino acids with a predicted molecular mass of ...
chapter two neurons and glia
... We begin our tour at the soma, the roughly spherical central part of the neuron. The cell body of the typical neuron is about 20 mm in diameter. The watery fluid inside the cell, called the cytosol, is a salty, potassiumrich solution that is separated from the outside by the neuronal membrane. Withi ...
... We begin our tour at the soma, the roughly spherical central part of the neuron. The cell body of the typical neuron is about 20 mm in diameter. The watery fluid inside the cell, called the cytosol, is a salty, potassiumrich solution that is separated from the outside by the neuronal membrane. Withi ...
PDF
... 1995; Girton and Johansen, 2008). If JIL-1 had a crucial role in promoting transcription at a majority of genes by regulating transcriptional elongation, it is difficult to envisage how lethality can be rescued to near wild-type levels in the complete absence of JIL-1 and interphase histone H3S10 ph ...
... 1995; Girton and Johansen, 2008). If JIL-1 had a crucial role in promoting transcription at a majority of genes by regulating transcriptional elongation, it is difficult to envisage how lethality can be rescued to near wild-type levels in the complete absence of JIL-1 and interphase histone H3S10 ph ...
A simple set of rules for primer sequence design is as follows
... sequester the cation; of these, dNTPs are the most concentrated, so [Mg2+] should be 0.5 2.5mM greater than [dNTP]. A titration should be performed with varying [Mg2+] with ...
... sequester the cation; of these, dNTPs are the most concentrated, so [Mg2+] should be 0.5 2.5mM greater than [dNTP]. A titration should be performed with varying [Mg2+] with ...
Primary transcript
A primary transcript is the single-stranded ribonucleic acid (RNA) product synthesized by transcription of DNA, and processed to yield various mature RNA products such as mRNAs, tRNAs, and rRNAs. The primary transcripts designated to be mRNAs are modified in preparation for translation. For example, a precursor messenger RNA (pre-mRNA) is a type of primary transcript that becomes a messenger RNA (mRNA) after processing.There are several steps contributing to the production of primary transcripts. All these steps involve a series of interactions to initiate and complete the transcription of DNA in the nucleus of eukaryotes. Certain factors play key roles in the activation and inhibition of transcription, where they regulate primary transcript production. Transcription produces primary transcripts that are further modified by several processes. These processes include the 5' cap, 3'-polyadenylation, and alternative splicing. In particular, alternative splicing directly contributes to the diversity of mRNA found in cells. The modifications of primary transcripts have been further studied in research seeking greater knowledge of the role and significance of these transcripts. Experimental studies based on molecular changes to primary transcripts the processes before and after transcription have led to greater understanding of diseases involving primary transcripts.