FISH or CISH methods for In situ hybridization
... In addition to reagents and kits for FISH and CISH, Invitrogen offers accessories and ancillary products such as the SPoT-Light® CISH™ Hybridizer, a hands-free denaturation and hybridization system. Figure 3—Advanced multiplexing capabilities achieved using a combination of FISH Tag™ and TSA™ Kits. ...
... In addition to reagents and kits for FISH and CISH, Invitrogen offers accessories and ancillary products such as the SPoT-Light® CISH™ Hybridizer, a hands-free denaturation and hybridization system. Figure 3—Advanced multiplexing capabilities achieved using a combination of FISH Tag™ and TSA™ Kits. ...
View poster
... Structural variations in the genome can be determined from NGS data with either whole genome sequencing (WGS) or targeted enrichment using exome or gene panels. Copy number variation (CNV) of genomic segments is a large category of structural variation and has been implicated in many Mendelian disea ...
... Structural variations in the genome can be determined from NGS data with either whole genome sequencing (WGS) or targeted enrichment using exome or gene panels. Copy number variation (CNV) of genomic segments is a large category of structural variation and has been implicated in many Mendelian disea ...
A haploid-specific transcriptional response to
... are attributed as the original place of publication with the correct citation details given; if an article is subsequently reproduced or disseminated not in its entirety but only in part or as a derivative work this must be clearly indicated. For commercial re-use, please contact journals.permission ...
... are attributed as the original place of publication with the correct citation details given; if an article is subsequently reproduced or disseminated not in its entirety but only in part or as a derivative work this must be clearly indicated. For commercial re-use, please contact journals.permission ...
Genetics Review Questions PPT
... C. Mutations that occur in gametes are NOT passed on to future generations. D. Mutations that occur in somatic cells are passed on to the future generations. ...
... C. Mutations that occur in gametes are NOT passed on to future generations. D. Mutations that occur in somatic cells are passed on to the future generations. ...
Functions of the nonsense-mediated RNA decay pathway in Drosophila development. PLoS Genetics 2 , 2143-2154.
... (A) FLP/MARCM system used to generate GFP-labeled homozygous mutant tracheal cell clones in heterozygous animals. Heterozygous cells have mutations (asterisk) in trans to a GAL80 transgene (triangle) that inhibits expression of a tracheal-specific, btl-GAL4-driven GFP transgene on another chromosome ...
... (A) FLP/MARCM system used to generate GFP-labeled homozygous mutant tracheal cell clones in heterozygous animals. Heterozygous cells have mutations (asterisk) in trans to a GAL80 transgene (triangle) that inhibits expression of a tracheal-specific, btl-GAL4-driven GFP transgene on another chromosome ...
Slides
... • Calorie consumption dropped from 2,000 to 500 per day for 4.5 million. • Children born or raised in this time were small, short in stature and had many diseases including, edema, anemia, diabetes and depression. • The Dutch Famine Birth Cohort study showed that women living during this time had ch ...
... • Calorie consumption dropped from 2,000 to 500 per day for 4.5 million. • Children born or raised in this time were small, short in stature and had many diseases including, edema, anemia, diabetes and depression. • The Dutch Famine Birth Cohort study showed that women living during this time had ch ...
AthaMap web tools for database-assisted identification of
... behind the factor name (Table 1). The binding sites were taken directly from the published literature, which is regularly screened in the process of updating the TRANSFAC1 database with plant transcription factor data (2). The screens were performed on the most recent version of the A.thaliana genom ...
... behind the factor name (Table 1). The binding sites were taken directly from the published literature, which is regularly screened in the process of updating the TRANSFAC1 database with plant transcription factor data (2). The screens were performed on the most recent version of the A.thaliana genom ...
Analysis of acid-induced asr gene promoter of Enterobacteriaceae
... region upstream –38 does not closely resemble the PhoB binding site (pho box). Transcription factor binding sites in bacterial genomes are usually long, consisting of ~30 bases and variable. Often most of their regulatory sequences are carried in two conserved subregions, each about 6 bases in lengt ...
... region upstream –38 does not closely resemble the PhoB binding site (pho box). Transcription factor binding sites in bacterial genomes are usually long, consisting of ~30 bases and variable. Often most of their regulatory sequences are carried in two conserved subregions, each about 6 bases in lengt ...
Gene silencing in mammalian cells and the spread of DNA
... Santos et al., 2002). Remethylation of most of the genome occurs after the blastocyst implants (Monk et al., 1987; Okano et al., 1999; Santos et al., 2002) and continues at a slower pace during the remainder of development. It is believed that this demethylation/ remethylation process converts gamet ...
... Santos et al., 2002). Remethylation of most of the genome occurs after the blastocyst implants (Monk et al., 1987; Okano et al., 1999; Santos et al., 2002) and continues at a slower pace during the remainder of development. It is believed that this demethylation/ remethylation process converts gamet ...
Table S3. Transcription factor binding sites identified in haplotypes
... at least 5 bases) and MATCHTM public 1.0 [104] (using 70% as a minimum overall similarity and 100% as core similarity cut-offs). ftz is a segmentation gene with neurogenic involvement it is known to be active late in development [105] but not studied during metamorphosis; although it wouldn´t be unw ...
... at least 5 bases) and MATCHTM public 1.0 [104] (using 70% as a minimum overall similarity and 100% as core similarity cut-offs). ftz is a segmentation gene with neurogenic involvement it is known to be active late in development [105] but not studied during metamorphosis; although it wouldn´t be unw ...
PDF
... more effectively than the Zfy1 transgene. Both transgenes are single copy and inserted on the X chromosome, but we cannot assess relative transcript levels in interphasic secondary spermatocytes because of our inability to adequately purify this rare cell type. However we have previously established ...
... more effectively than the Zfy1 transgene. Both transgenes are single copy and inserted on the X chromosome, but we cannot assess relative transcript levels in interphasic secondary spermatocytes because of our inability to adequately purify this rare cell type. However we have previously established ...
Comparative Genome and Proteome Analysis of Anopheles
... • They largely belong to two distinct speciesspecific clades • Identified only two 1:1 orthologous ...
... • They largely belong to two distinct speciesspecific clades • Identified only two 1:1 orthologous ...
j - Computer Science & Engineering
... – Mutations at the single character level are frequent. – Does anybody know what these are called? makes it difficult to determine evolutionary relationship at the DNA sequence level. – Large gaps occur less frequently. gap features can be used to recognize similarity over long periods of time. ...
... – Mutations at the single character level are frequent. – Does anybody know what these are called? makes it difficult to determine evolutionary relationship at the DNA sequence level. – Large gaps occur less frequently. gap features can be used to recognize similarity over long periods of time. ...
Garrett-Engele* C. M., Siegal*, M. L., Manoli, D. S., Williams, B. C., Li. H., and Baker, B. S.
... Fig. 1. The Drosophila somatic sex-determination hierarchy. The ratio of X chromosomes to sets of autosomes determines the on/off state of the Sex-lethal (Sxl) gene. In females, where the X:A ratio is 1, active SXL protein is made and its production is maintained via autoregulation. The presence of ...
... Fig. 1. The Drosophila somatic sex-determination hierarchy. The ratio of X chromosomes to sets of autosomes determines the on/off state of the Sex-lethal (Sxl) gene. In females, where the X:A ratio is 1, active SXL protein is made and its production is maintained via autoregulation. The presence of ...
Reverse Genetic Analysis of Terminal Ear
... support models in which these genes help maintain cells in a pluripotent state. For the first part of the thesis, analysis of lines carrying single knockouts of TEL1 and TEL2 and double knockout lines reveals a slightly accelerated rate of organogenesis, consistent with these genes normally acting t ...
... support models in which these genes help maintain cells in a pluripotent state. For the first part of the thesis, analysis of lines carrying single knockouts of TEL1 and TEL2 and double knockout lines reveals a slightly accelerated rate of organogenesis, consistent with these genes normally acting t ...
Genetic and Genomic Approaches of Asthma: New
... DNase I hypersensitive sites (DHSs) provide important information on the presence of transcriptional regulatory elements and the state of chromatin in mammalian cells. Single-cell DNase sequencing can detect genome-wide DHSs at the single-cell level. The DHSs are highly reproducible among individual ...
... DNase I hypersensitive sites (DHSs) provide important information on the presence of transcriptional regulatory elements and the state of chromatin in mammalian cells. Single-cell DNase sequencing can detect genome-wide DHSs at the single-cell level. The DHSs are highly reproducible among individual ...
Sex determination in Bombyx mori
... the genes contain a DM domain, a zinc finger-like DNAbinding motif35,36. Furthermore, the two genes directly regulate yolk protein gene transcription37,38 . The homologues of dsx have also been found in human, mouse, chicken, and turtle, among vertebrates. Most dsx homologues are considered to regul ...
... the genes contain a DM domain, a zinc finger-like DNAbinding motif35,36. Furthermore, the two genes directly regulate yolk protein gene transcription37,38 . The homologues of dsx have also been found in human, mouse, chicken, and turtle, among vertebrates. Most dsx homologues are considered to regul ...
Conditions for gene disruption by homologous
... cells reached an OD600nm of about 0.4 after 7 to 14 days. Subsequently, cells were spread on lactose and tryptone plates and sprayed with 5 mg ml –1 X-gal after 7 days of growth. As shown in Table 1, in only two of the tested conditions were blue colonies obtained, indicating the presence of lacS in ...
... cells reached an OD600nm of about 0.4 after 7 to 14 days. Subsequently, cells were spread on lactose and tryptone plates and sprayed with 5 mg ml –1 X-gal after 7 days of growth. As shown in Table 1, in only two of the tested conditions were blue colonies obtained, indicating the presence of lacS in ...
De novo DNA cytosine methyltransferase activities in
... cells have been shown to have relatively high de novo methylation activity (Stewart et al., 1982; and this study) while the de novo methylation activity is low or undetectable in differentiated EC cells, postgastrulation embryos and adult somatic tissues (Jähner et al., 1982; Stewart et al., 1982; r ...
... cells have been shown to have relatively high de novo methylation activity (Stewart et al., 1982; and this study) while the de novo methylation activity is low or undetectable in differentiated EC cells, postgastrulation embryos and adult somatic tissues (Jähner et al., 1982; Stewart et al., 1982; r ...
Insuperable Problems Of The Genetic Code Initially Emerging In An
... A companion paper (Carter and Wills, 2017) outlines evidence tracing the origin of genetic coding to ancestral enzymic aaRS, and provides detailed justifications for the conclusion that such ...
... A companion paper (Carter and Wills, 2017) outlines evidence tracing the origin of genetic coding to ancestral enzymic aaRS, and provides detailed justifications for the conclusion that such ...
Insuperable Problems Of The Genetic Code Initially
... A companion paper (Carter and Wills, 2017) outlines evidence tracing the origin of genetic coding to ancestral enzymic aaRS, and provides detailed justifications for the conclusion that such ...
... A companion paper (Carter and Wills, 2017) outlines evidence tracing the origin of genetic coding to ancestral enzymic aaRS, and provides detailed justifications for the conclusion that such ...
Novel Molecular Methods for Discovery and Engineering of
... environmental sample is recommended instead of DNA isolation. This is related to the presence of intervening non-coding sequences (introns) in most of eukaryotic genes, which are not present in the corresponding mature messenger RNAs (mRNAs). Then mRNAs are purified from the total RNA and used as th ...
... environmental sample is recommended instead of DNA isolation. This is related to the presence of intervening non-coding sequences (introns) in most of eukaryotic genes, which are not present in the corresponding mature messenger RNAs (mRNAs). Then mRNAs are purified from the total RNA and used as th ...
Specific Combinations of Zein Genes and Genetic Backgrounds
... either be almost null for the zH1 and zH2 or contain one or both the two zH classes in variable relative amounts. In most cases this correlates with the levels of the heavy type zein transcripts suggesting that some zH genes may be regulated by factors other than O2. Moreover, in some crosses betwee ...
... either be almost null for the zH1 and zH2 or contain one or both the two zH classes in variable relative amounts. In most cases this correlates with the levels of the heavy type zein transcripts suggesting that some zH genes may be regulated by factors other than O2. Moreover, in some crosses betwee ...
NIH Public Access
... (or resistance) of particular genes to silencing remains poorly understood. In recent years substantial progress has been made in defining the series of heterochromatic chromatin modifications that spread across the chromosome shortly after XIST RNA first paints the chromosome (reviewed in (Heard, 2 ...
... (or resistance) of particular genes to silencing remains poorly understood. In recent years substantial progress has been made in defining the series of heterochromatic chromatin modifications that spread across the chromosome shortly after XIST RNA first paints the chromosome (reviewed in (Heard, 2 ...
The Nucleotide and Derived Amino Acid
... apo-A-IV contained 376 residues. Throughout most of quence and organization of the docosapeptide units are reits length, human apo-A-IVwas found to contain mul- markably homologous to the tandemly repeated segments tiple tandem 22-residue repeated segments having am- found in apo-A-I (7,9),which sug ...
... apo-A-IV contained 376 residues. Throughout most of quence and organization of the docosapeptide units are reits length, human apo-A-IVwas found to contain mul- markably homologous to the tandemly repeated segments tiple tandem 22-residue repeated segments having am- found in apo-A-I (7,9),which sug ...
Primary transcript
A primary transcript is the single-stranded ribonucleic acid (RNA) product synthesized by transcription of DNA, and processed to yield various mature RNA products such as mRNAs, tRNAs, and rRNAs. The primary transcripts designated to be mRNAs are modified in preparation for translation. For example, a precursor messenger RNA (pre-mRNA) is a type of primary transcript that becomes a messenger RNA (mRNA) after processing.There are several steps contributing to the production of primary transcripts. All these steps involve a series of interactions to initiate and complete the transcription of DNA in the nucleus of eukaryotes. Certain factors play key roles in the activation and inhibition of transcription, where they regulate primary transcript production. Transcription produces primary transcripts that are further modified by several processes. These processes include the 5' cap, 3'-polyadenylation, and alternative splicing. In particular, alternative splicing directly contributes to the diversity of mRNA found in cells. The modifications of primary transcripts have been further studied in research seeking greater knowledge of the role and significance of these transcripts. Experimental studies based on molecular changes to primary transcripts the processes before and after transcription have led to greater understanding of diseases involving primary transcripts.