GENETIC ANALYSIS OF THE FUNCTION OF THE DROSOPHILA
... the next section I will discuss a family of proteins that share a conserved DNA binding domain with doublesex and are found in a range of higher organisms. ...
... the next section I will discuss a family of proteins that share a conserved DNA binding domain with doublesex and are found in a range of higher organisms. ...
Package ‘GenomicFeatures’ October 14, 2014
... For asBED, a GRanges, with the columns name, thickStart, thickEnd, blockStarts, blockSizes added. The thick regions correspond to the CDS regions, and the blocks represent the exons. The transcript IDs are stored in the name column. The ranges are the transcript bounds. For asGFF, a GRanges, with co ...
... For asBED, a GRanges, with the columns name, thickStart, thickEnd, blockStarts, blockSizes added. The thick regions correspond to the CDS regions, and the blocks represent the exons. The transcript IDs are stored in the name column. The ranges are the transcript bounds. For asGFF, a GRanges, with co ...
Immunogenetics
... required to assemble a functional heavy chain V region gene, are governed by the 7-9 and the 12-23 rules. Explain. Discuss four mechanisms that contribute to antibody diversity. Describe the differential splicing of RNA that progresses from membrane bound IgM to secreted IgM. ...
... required to assemble a functional heavy chain V region gene, are governed by the 7-9 and the 12-23 rules. Explain. Discuss four mechanisms that contribute to antibody diversity. Describe the differential splicing of RNA that progresses from membrane bound IgM to secreted IgM. ...
Genome engineering of mammalian haploid embryonic stem cells
... repeats (CRISPR) RNA-guided Cas9 nucleases (Cong et al., 2013; Mali et al., 2013). Zinc-finger nucleases and transcription activator-like effector nucleases are composed of programmable, sequence-specific DNA-binding modules linked to a non-specific DNA cleavage domain. CRISPR RNA-guided Cas9 nuclea ...
... repeats (CRISPR) RNA-guided Cas9 nucleases (Cong et al., 2013; Mali et al., 2013). Zinc-finger nucleases and transcription activator-like effector nucleases are composed of programmable, sequence-specific DNA-binding modules linked to a non-specific DNA cleavage domain. CRISPR RNA-guided Cas9 nuclea ...
Genetic recombination and mutations - formatted
... One must examine the concepts in the light of the functional capability of the genome. As a safeguard against the possible deleterious effects of mutation, nature has evolved an elaborate mechanism of damage control through repair or rectification of mistakes that take place during the replication p ...
... One must examine the concepts in the light of the functional capability of the genome. As a safeguard against the possible deleterious effects of mutation, nature has evolved an elaborate mechanism of damage control through repair or rectification of mistakes that take place during the replication p ...
CHARACTERlZATION OF THE ~ 0 CHONDRIA . L DNA MOLECULE
... structural gene. The sea anemone Metridium senile mtDNA codes for only 2 M A Sof the 22 tRNAs required for decoding the mitochondrial genetic code: one for tryptophane and ...
... structural gene. The sea anemone Metridium senile mtDNA codes for only 2 M A Sof the 22 tRNAs required for decoding the mitochondrial genetic code: one for tryptophane and ...
Diplosporous development in Boehmeria tricuspis: Insights
... Boehmeria tricuspis includes sexually reproducing diploid and apomictic triploid individuals. Previously, we established that triploid B. tricuspis reproduces through obligate diplospory. To understand the molecular basis of apomictic development in B. tricuspis, we sequenced and compared transcript ...
... Boehmeria tricuspis includes sexually reproducing diploid and apomictic triploid individuals. Previously, we established that triploid B. tricuspis reproduces through obligate diplospory. To understand the molecular basis of apomictic development in B. tricuspis, we sequenced and compared transcript ...
doc THREE finals
... (a) A yeast origin of replication, two yeast telomeric sequences, and one selectable marker (such as URA3+) are needed in a vector to be grown in yeast. (b) Genomic libraries can be made by generating genomic DNA fragments without the use of restriction endonucleases. (c) A cosmid vector is a plasmi ...
... (a) A yeast origin of replication, two yeast telomeric sequences, and one selectable marker (such as URA3+) are needed in a vector to be grown in yeast. (b) Genomic libraries can be made by generating genomic DNA fragments without the use of restriction endonucleases. (c) A cosmid vector is a plasmi ...
as a PDF
... Postgenomic studies constitute one of the current challenges in biology due to the burgeoning quantity of genome sequence available and the difficulties in utilizing the data to understand how an organism functions. A major problem is to couple the DNA sequences with the battery of proteins a given ...
... Postgenomic studies constitute one of the current challenges in biology due to the burgeoning quantity of genome sequence available and the difficulties in utilizing the data to understand how an organism functions. A major problem is to couple the DNA sequences with the battery of proteins a given ...
Plasmids
... strain of Escherichia coli. E. coli is a proteobacterium that normally inhabits the intestinal tract of warm-blooded mammals. The virulent strains of E. coli that appear in the news have acquired, often by lateral gene transfer, pathogenicity islands containing genes for virulence factors, toxins an ...
... strain of Escherichia coli. E. coli is a proteobacterium that normally inhabits the intestinal tract of warm-blooded mammals. The virulent strains of E. coli that appear in the news have acquired, often by lateral gene transfer, pathogenicity islands containing genes for virulence factors, toxins an ...
Motifs and motif prediction methods I - BIDD
... •Restricted to key conserved features in order to reduce the “noise” level •Built by hand in a stepwise fashion from multiple alignments ...
... •Restricted to key conserved features in order to reduce the “noise” level •Built by hand in a stepwise fashion from multiple alignments ...
Disrupting antibiotic resistance propagation by inhibiting
... coli F plasmid by Lederberg and Tatum in 1946 (2). Conjugative DNA transfer is also the central mechanism by which antibiotic resistance and virulence factors are propagated in bacterial populations (reviewed in ref. 3). Indeed, it is well established that antibiotic resistance can be rapidly acquir ...
... coli F plasmid by Lederberg and Tatum in 1946 (2). Conjugative DNA transfer is also the central mechanism by which antibiotic resistance and virulence factors are propagated in bacterial populations (reviewed in ref. 3). Indeed, it is well established that antibiotic resistance can be rapidly acquir ...
LECTURE 1 Human Chromosomes Human Karyotype
... By the end of this lecture, the students should be able to: Describe the number, structure, and classification of human chromosomes. Explain what a Karyotype is and how it is obtained. Describe chromosomal banding and explain its use. Describe the process of in situ hybridization and the infor ...
... By the end of this lecture, the students should be able to: Describe the number, structure, and classification of human chromosomes. Explain what a Karyotype is and how it is obtained. Describe chromosomal banding and explain its use. Describe the process of in situ hybridization and the infor ...
Characterization of an IS-like element from
... frame (ORF) coding for a hypothetical protein with sequence homologies to proteins of known IS elements was identified (see below). The entire ORF was cloned by screening a M . tuberculosis library in A2001 (Vismara et al., 1990) using the cloned EcoRI fragment of the Agtl 1 library containing the O ...
... frame (ORF) coding for a hypothetical protein with sequence homologies to proteins of known IS elements was identified (see below). The entire ORF was cloned by screening a M . tuberculosis library in A2001 (Vismara et al., 1990) using the cloned EcoRI fragment of the Agtl 1 library containing the O ...
Analysis of cell division parameters and cell cycle gene expression
... cycle phase durations. Subsequently, this model system was used to follow the transcription profile of key cell cycle genes during a complete cultivation cycle. According to the calculated changes in the relative division rate over time, the cell cycle genes could be classified into four groups base ...
... cycle phase durations. Subsequently, this model system was used to follow the transcription profile of key cell cycle genes during a complete cultivation cycle. According to the calculated changes in the relative division rate over time, the cell cycle genes could be classified into four groups base ...
Members of the RKD transcription factor family induce an egg cell
... on the notion that most cDNA libraries are made from tissues or plant organs in which egg cells and their transcripts are highly diluted or not present, the analysis was focused on 125 unique sequences which did not show any significant sequence similarity to more than one million publicly available ...
... on the notion that most cDNA libraries are made from tissues or plant organs in which egg cells and their transcripts are highly diluted or not present, the analysis was focused on 125 unique sequences which did not show any significant sequence similarity to more than one million publicly available ...
Yeast genetics to investigate the function of core pre
... allowed investigation of genetic interactions between core splicing machinery. Synthetic enhancement/lethality occurs when one gene mutation exacerbates the severity of another gene mutation. Alternatively, a second mutation may suppress the phenotype of the first mutation. Traditionally this type o ...
... allowed investigation of genetic interactions between core splicing machinery. Synthetic enhancement/lethality occurs when one gene mutation exacerbates the severity of another gene mutation. Alternatively, a second mutation may suppress the phenotype of the first mutation. Traditionally this type o ...
A model for repair of radiation-induced DNA double
... overlapping DNA fragments. Not only are the number of fragments generated per chromosome enormous, but from the logistical point of view of a particular searching fragment, there is no obvious way of eliminating from the search other fragments that have already been scrutinized, resulting in repetit ...
... overlapping DNA fragments. Not only are the number of fragments generated per chromosome enormous, but from the logistical point of view of a particular searching fragment, there is no obvious way of eliminating from the search other fragments that have already been scrutinized, resulting in repetit ...
Overexpression of miR165 Affects Apical
... species and, of them, many putative target genes have been predicted (Dugas and Bartel 2004, Kidner and Martienssen 2005a, Zhang et al. 2006). It has been shown that miRNAs are first transcribed by RNA polymerase II to produce pri-miRNAs, which are then processed by DICER-like proteins and other com ...
... species and, of them, many putative target genes have been predicted (Dugas and Bartel 2004, Kidner and Martienssen 2005a, Zhang et al. 2006). It has been shown that miRNAs are first transcribed by RNA polymerase II to produce pri-miRNAs, which are then processed by DICER-like proteins and other com ...
Chpt15_PosNegCntrl.doc
... (lactose permease) and initial breakdown of lactose (the disaccharide -Dgalactosyl-1->4-D-glucose) into galactose and glucose (catalyzed by galactosidase). These monosaccharides are broken down to lactate (principally via glycolysis, producing ATP), and from lactate to CO2 (via the citric acid cyc ...
... (lactose permease) and initial breakdown of lactose (the disaccharide -Dgalactosyl-1->4-D-glucose) into galactose and glucose (catalyzed by galactosidase). These monosaccharides are broken down to lactate (principally via glycolysis, producing ATP), and from lactate to CO2 (via the citric acid cyc ...
Molecular and Cellular Biology, September 2000, p
... dependent activity of the histone H4 gene promoter (24, 43). All highly conserved nucleotide motifs in the consensus H4 site II sequence are absolutely conserved in the proximal promoter region of all known vertebrate H10 genes (Fig. 1B). These observations strongly suggest that at least in prolife ...
... dependent activity of the histone H4 gene promoter (24, 43). All highly conserved nucleotide motifs in the consensus H4 site II sequence are absolutely conserved in the proximal promoter region of all known vertebrate H10 genes (Fig. 1B). These observations strongly suggest that at least in prolife ...
HMMs for gene predictions.
... genomic DNA is referred to as a signal, whereas a region of genomic DNA is a content. • Examples of signals: splice sites, starts and ends of transcription or translation, branch points, transcription factor binding sites • Examples of contents: exons, introns, UTRs, promoter regions ...
... genomic DNA is referred to as a signal, whereas a region of genomic DNA is a content. • Examples of signals: splice sites, starts and ends of transcription or translation, branch points, transcription factor binding sites • Examples of contents: exons, introns, UTRs, promoter regions ...
Primary transcript
A primary transcript is the single-stranded ribonucleic acid (RNA) product synthesized by transcription of DNA, and processed to yield various mature RNA products such as mRNAs, tRNAs, and rRNAs. The primary transcripts designated to be mRNAs are modified in preparation for translation. For example, a precursor messenger RNA (pre-mRNA) is a type of primary transcript that becomes a messenger RNA (mRNA) after processing.There are several steps contributing to the production of primary transcripts. All these steps involve a series of interactions to initiate and complete the transcription of DNA in the nucleus of eukaryotes. Certain factors play key roles in the activation and inhibition of transcription, where they regulate primary transcript production. Transcription produces primary transcripts that are further modified by several processes. These processes include the 5' cap, 3'-polyadenylation, and alternative splicing. In particular, alternative splicing directly contributes to the diversity of mRNA found in cells. The modifications of primary transcripts have been further studied in research seeking greater knowledge of the role and significance of these transcripts. Experimental studies based on molecular changes to primary transcripts the processes before and after transcription have led to greater understanding of diseases involving primary transcripts.