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... silencing complex (RISC); blue ribbon)] (d & e). The dFmrp–mRNP complex could be transported (f) into dendrites after the dFmrpmRNP has been assembled into a large transport complex which is shown with details in the dashed square. Once transported into the postsynaptic region, both complexes can be ...
... silencing complex (RISC); blue ribbon)] (d & e). The dFmrp–mRNP complex could be transported (f) into dendrites after the dFmrpmRNP has been assembled into a large transport complex which is shown with details in the dashed square. Once transported into the postsynaptic region, both complexes can be ...
Transgenic Tobacco Plants Expressing the Drosophila
... sue at frequencies of 21 to 36% for leaf phenotypes and 1 to 2% for flower phenotypes, but they were not observed in SR1 control plants or in F1 progeny plants that did not contain the transgene or did not express it (Table 1). Transformants expressing the PCcd–GFP construct also showed an increase ...
... sue at frequencies of 21 to 36% for leaf phenotypes and 1 to 2% for flower phenotypes, but they were not observed in SR1 control plants or in F1 progeny plants that did not contain the transgene or did not express it (Table 1). Transformants expressing the PCcd–GFP construct also showed an increase ...
Frequent and histological type-specific inactivation of 14-3
... mers, suggesting `partial' methylation. In total, nine SCLC cell lines (69%) demonstrated hypermethylation of the 14-3-3s gene. The other four SCLC cell lines showed unmethylated patterns. In addition to the SCLC lines, large cell lung cancer lines among NSCLC cell lines also frequently showed hyper ...
... mers, suggesting `partial' methylation. In total, nine SCLC cell lines (69%) demonstrated hypermethylation of the 14-3-3s gene. The other four SCLC cell lines showed unmethylated patterns. In addition to the SCLC lines, large cell lung cancer lines among NSCLC cell lines also frequently showed hyper ...
- Wiley Online Library
... silencing complex (RISC); blue ribbon)] (d & e). The dFmrp–mRNP complex could be transported (f) into dendrites after the dFmrpmRNP has been assembled into a large transport complex which is shown with details in the dashed square. Once transported into the postsynaptic region, both complexes can be ...
... silencing complex (RISC); blue ribbon)] (d & e). The dFmrp–mRNP complex could be transported (f) into dendrites after the dFmrpmRNP has been assembled into a large transport complex which is shown with details in the dashed square. Once transported into the postsynaptic region, both complexes can be ...
Transcriptional Repression of the
... domains are critical. The requirement for the DBD was further substantiated using a point mutant that has a cysteine-to-alanine mutation within the first zinc finger (C576A). This mutation, which disrupts DNA-binding activity, eliminated repression by the receptor. A fourth mutation, which deletes t ...
... domains are critical. The requirement for the DBD was further substantiated using a point mutant that has a cysteine-to-alanine mutation within the first zinc finger (C576A). This mutation, which disrupts DNA-binding activity, eliminated repression by the receptor. A fourth mutation, which deletes t ...
Expression of death-related genes and reactive oxygen species
... h after inoculation and to zero 24 h later (Fig. 1). ScDSP-1 and ScDSP-2 gene expression and ROS levels increase in S. tropicum cultures exposed to different concentrations of OCTA. A peak in ScDSP-1 and ScDSP2 expression was visible as early as 1 h after exposure and its intensity for cells in LE e ...
... h after inoculation and to zero 24 h later (Fig. 1). ScDSP-1 and ScDSP-2 gene expression and ROS levels increase in S. tropicum cultures exposed to different concentrations of OCTA. A peak in ScDSP-1 and ScDSP2 expression was visible as early as 1 h after exposure and its intensity for cells in LE e ...
Application of Bruchin B to pea pods results in
... from pea pods treated either with Bruchin B or ethanol, yielded 19 amplification products whose appearance was either bruchin treatment-specific or genotype-specific (data not shown). One of these, designated GT13, obtained with the primer combination T11GT/OPA-13, appeared to have been derived from ...
... from pea pods treated either with Bruchin B or ethanol, yielded 19 amplification products whose appearance was either bruchin treatment-specific or genotype-specific (data not shown). One of these, designated GT13, obtained with the primer combination T11GT/OPA-13, appeared to have been derived from ...
Globin gene family
... plays important roles in the cell • For example, genomes of humans, rats, and mice show high sequence conservation for about 500 noncoding regions • Sequencing of the human genome reveals that 98.5% does not code for proteins, rRNAs, or tRNAs Copyright © 2008 Pearson Education Inc., publishing as Pe ...
... plays important roles in the cell • For example, genomes of humans, rats, and mice show high sequence conservation for about 500 noncoding regions • Sequencing of the human genome reveals that 98.5% does not code for proteins, rRNAs, or tRNAs Copyright © 2008 Pearson Education Inc., publishing as Pe ...
Control of Growth and of the Nuclear Division Cycle in Neurospora
... that three subunits, among the small-molecularweight components, appear to be common to the two enzymes (24). It has been suggested that the common subunits perform the same basic function during transcription. Since the production of bacterial RNA polymerase seems to be autoregulated (62, 66, 78, 1 ...
... that three subunits, among the small-molecularweight components, appear to be common to the two enzymes (24). It has been suggested that the common subunits perform the same basic function during transcription. Since the production of bacterial RNA polymerase seems to be autoregulated (62, 66, 78, 1 ...
Identification of R-Gene Homologous DNA Fragments Genetically
... grouped in three classes (C1 [0.5 kb]; L1, L2, C2, C3 [0.5 kb]; and L3, L4, C4 [0.8 kb]) based on their DNA sequence similarities. All sequences have the RG1 primer sequence on one end and the RG2 sequence on the other end. Upon screening GenBank with BLASTX (Altschul et al. 1990) for similar sequen ...
... grouped in three classes (C1 [0.5 kb]; L1, L2, C2, C3 [0.5 kb]; and L3, L4, C4 [0.8 kb]) based on their DNA sequence similarities. All sequences have the RG1 primer sequence on one end and the RG2 sequence on the other end. Upon screening GenBank with BLASTX (Altschul et al. 1990) for similar sequen ...
The distribution of DNA translocation times in solid
... chamber was about 10 nM in this work. The salt solution used for the experiments was 1 M KCl or 1.6 M KCl plus glycerol with 10 mM TE buffer at pH 7.5 ± 0.5. The data used in this work were measured with DNA molecules between 2.2 kbp (chain length L c = 738 nm) and 23 kbp ( L c = 7820 nm). The chain ...
... chamber was about 10 nM in this work. The salt solution used for the experiments was 1 M KCl or 1.6 M KCl plus glycerol with 10 mM TE buffer at pH 7.5 ± 0.5. The data used in this work were measured with DNA molecules between 2.2 kbp (chain length L c = 738 nm) and 23 kbp ( L c = 7820 nm). The chain ...
Characterization of Two Rice MADS Box Genes That Control
... transgene expression and the phenotypes were co-inherited with the kanamycin resistant gene to the next generation. These results indicate that OsMADS7 and 8 are structurally related to the AGL2 family and are involved in controlling flowering time. ...
... transgene expression and the phenotypes were co-inherited with the kanamycin resistant gene to the next generation. These results indicate that OsMADS7 and 8 are structurally related to the AGL2 family and are involved in controlling flowering time. ...
Is HP1 an RNA detector that functions both in repression and
... chromosomes and how it might specify distinct activities at distinct sites. HP1 is now known to be a highly interactive protein; it is capable of interacting with a host of proteins with a range of nuclear activities (Kellum, 2003; and references therein). Do RNA sense strands recruit transcriptiona ...
... chromosomes and how it might specify distinct activities at distinct sites. HP1 is now known to be a highly interactive protein; it is capable of interacting with a host of proteins with a range of nuclear activities (Kellum, 2003; and references therein). Do RNA sense strands recruit transcriptiona ...
Paper - Ran Blekhman
... Individual TFBSs are highly non-randomly spatially distributed in metazoans, and are typically located within clusters of other binding sites termed cis-regulatory modules (CRMs). These clusters serve to bind multiple TFs whose combined action controls the level and location of gene expression. We i ...
... Individual TFBSs are highly non-randomly spatially distributed in metazoans, and are typically located within clusters of other binding sites termed cis-regulatory modules (CRMs). These clusters serve to bind multiple TFs whose combined action controls the level and location of gene expression. We i ...
Inhibition of RNA Synthesis by Anthracycline Analogs
... the appearance of chromatin as well as the shape and size of the nucleoli. The changes appear soon after daunomycin addition to the medium of cell culture. ...
... the appearance of chromatin as well as the shape and size of the nucleoli. The changes appear soon after daunomycin addition to the medium of cell culture. ...
cis-Regulatory Elements and Chromatin State
... aligned sequences from 7.0 kb upstream of FT from A. thaliana accession Columbia (Col) (Figure 1A) to FT sequence from A. thaliana accession Landsberg erecta (Ler) and FT homologous genes from Arabidopsis lyrata, Brassica rapa, and Arabis alpina (Aa FT1-Aa FT3). The pairwise alignment revealed three ...
... aligned sequences from 7.0 kb upstream of FT from A. thaliana accession Columbia (Col) (Figure 1A) to FT sequence from A. thaliana accession Landsberg erecta (Ler) and FT homologous genes from Arabidopsis lyrata, Brassica rapa, and Arabis alpina (Aa FT1-Aa FT3). The pairwise alignment revealed three ...
Spr01Final Exam Answer Key
... products. How does phage λ regulate the expression of these genes such that int and xis are only expressed at the proper time? (4pts) Before integratation, the transcripts from pL containing int and xis are degraded because they also contain the RNaseIII degradation signal. Only transcripts from pI, ...
... products. How does phage λ regulate the expression of these genes such that int and xis are only expressed at the proper time? (4pts) Before integratation, the transcripts from pL containing int and xis are degraded because they also contain the RNaseIII degradation signal. Only transcripts from pI, ...
X inactivation Xplained
... Silencing of one X chromosome by Xist. (a) The two X chromosome territories are depicted. Xist RNA produced from the Xic locus accumulates on the future Xi. This leads to the formation of a nuclear compartment that is devoid of the transcription machinery, such as RNA polymerase II (Pol II). Polycom ...
... Silencing of one X chromosome by Xist. (a) The two X chromosome territories are depicted. Xist RNA produced from the Xic locus accumulates on the future Xi. This leads to the formation of a nuclear compartment that is devoid of the transcription machinery, such as RNA polymerase II (Pol II). Polycom ...
Newsletter 1
... common male ancestor. A similar test for a common female ancestor is also available using ‘mitochondrial’ DNA, but as names in our society pass down the male line, this is less useful for many family history purposes. Specific parts of the Y-chromosome known as ‘markers’ are used to make such compar ...
... common male ancestor. A similar test for a common female ancestor is also available using ‘mitochondrial’ DNA, but as names in our society pass down the male line, this is less useful for many family history purposes. Specific parts of the Y-chromosome known as ‘markers’ are used to make such compar ...
Mary Ann Osley*, Alastair Fleming, and Cheng
... transferred via a thioester bond to a cysteine residue in one of many different ubiquitin-conjugating enzymes (Ubcs/E2s). The ubiquitin moiety is transferred to the appropriate substrate through the intermediary of a large family of ubiquitin ligases (E3s), which contain several signature domains, i ...
... transferred via a thioester bond to a cysteine residue in one of many different ubiquitin-conjugating enzymes (Ubcs/E2s). The ubiquitin moiety is transferred to the appropriate substrate through the intermediary of a large family of ubiquitin ligases (E3s), which contain several signature domains, i ...
GENETIC VARIATION OF TASTE RECEPTORS Abstract
... sensitive (4). The AVI haplotypes in the non-tester differ at 3 SNPs from the PAV haplotypes of the tasters (9). The aim of this practical: To focus on the TAS2R38 genotype and its link with the ability to taste PTC test paper. The SNP at position 785 is of specific concern in genotyping. Comparing ...
... sensitive (4). The AVI haplotypes in the non-tester differ at 3 SNPs from the PAV haplotypes of the tasters (9). The aim of this practical: To focus on the TAS2R38 genotype and its link with the ability to taste PTC test paper. The SNP at position 785 is of specific concern in genotyping. Comparing ...
Primary transcript
A primary transcript is the single-stranded ribonucleic acid (RNA) product synthesized by transcription of DNA, and processed to yield various mature RNA products such as mRNAs, tRNAs, and rRNAs. The primary transcripts designated to be mRNAs are modified in preparation for translation. For example, a precursor messenger RNA (pre-mRNA) is a type of primary transcript that becomes a messenger RNA (mRNA) after processing.There are several steps contributing to the production of primary transcripts. All these steps involve a series of interactions to initiate and complete the transcription of DNA in the nucleus of eukaryotes. Certain factors play key roles in the activation and inhibition of transcription, where they regulate primary transcript production. Transcription produces primary transcripts that are further modified by several processes. These processes include the 5' cap, 3'-polyadenylation, and alternative splicing. In particular, alternative splicing directly contributes to the diversity of mRNA found in cells. The modifications of primary transcripts have been further studied in research seeking greater knowledge of the role and significance of these transcripts. Experimental studies based on molecular changes to primary transcripts the processes before and after transcription have led to greater understanding of diseases involving primary transcripts.