The Modular Structure and Function of the Wheat HI Promoter with S
... found in the promoter regions of the TH315 and TH325 genes (Fig. 1). The Oct motif is conserved in almost all the plant histone gene promoters known to date, and it has been shown to be a positive cis-acting element in some plant H3 and H4 genes (Chaubet et al. 1996, Nakayama et al. 1992, Terada et ...
... found in the promoter regions of the TH315 and TH325 genes (Fig. 1). The Oct motif is conserved in almost all the plant histone gene promoters known to date, and it has been shown to be a positive cis-acting element in some plant H3 and H4 genes (Chaubet et al. 1996, Nakayama et al. 1992, Terada et ...
DNA Mutation and Repair
... mutation rates low but accidents happen and • Some types of mutation are invisible to the polymerase • Strand slippage is common in repetitive regions of the genome • DNA forms a temporary hairpin • DNA slips along it’s length but no mismatch exists • Replication proceed but there is an increase or ...
... mutation rates low but accidents happen and • Some types of mutation are invisible to the polymerase • Strand slippage is common in repetitive regions of the genome • DNA forms a temporary hairpin • DNA slips along it’s length but no mismatch exists • Replication proceed but there is an increase or ...
A Mutation in the Eta Subunit of Pyruvate Dehydrogenase
... In previous studies, biochemical and immunologic experiments demonstrated that the proband had variable PD C activity in cells and tissues and that the level of activity corresponded to the amo un t of E] subunit proteins detected by immunoblot analysis (18). Whereas fibroblasts had normal activity ...
... In previous studies, biochemical and immunologic experiments demonstrated that the proband had variable PD C activity in cells and tissues and that the level of activity corresponded to the amo un t of E] subunit proteins detected by immunoblot analysis (18). Whereas fibroblasts had normal activity ...
Point mutation of bacterial artificial chromosomes by ET recombination
... (Muyrers et al., 2000a). Thus, the risk of unwanted intramolecular rearrangement is minimized, allowing recombinants that contain no other unintended changes to be recovered efficiently (Figure 2 and data not shown). (iii) ET recombination is transferable to the host strain in which the BAC resides, ...
... (Muyrers et al., 2000a). Thus, the risk of unwanted intramolecular rearrangement is minimized, allowing recombinants that contain no other unintended changes to be recovered efficiently (Figure 2 and data not shown). (iii) ET recombination is transferable to the host strain in which the BAC resides, ...
Tracking bacterial DNA replication forks in vivo by pulsed field gel
... acids. The dnaAls mutation is a mutation that affects DNA initiation (3). A temperature shift to the non-permissive temperature followed by a shift back to the permissive temperature aligns DNA replication forks at oriC. Since de novo protein synthesis is required for initiation of each new round of ...
... acids. The dnaAls mutation is a mutation that affects DNA initiation (3). A temperature shift to the non-permissive temperature followed by a shift back to the permissive temperature aligns DNA replication forks at oriC. Since de novo protein synthesis is required for initiation of each new round of ...
Ch 18 - Bob Bruner`s Chemistry and Molecular Biology Resources
... A consequence is that certain mutations that might naively appear to be neutral are not necessarily neutral. A change from one proline codon to another might seem neutral; however, if the change is from a codon that is easy to translate to one more difficult to translate, it might well slow down the ...
... A consequence is that certain mutations that might naively appear to be neutral are not necessarily neutral. A change from one proline codon to another might seem neutral; however, if the change is from a codon that is easy to translate to one more difficult to translate, it might well slow down the ...
Gene Section BOP1 (block of proliferation 1) Atlas of Genetics and Cytogenetics
... DGAT1, 8q24.3, diacylglycerol O-acyltransferase homolog 1 (mouse); FBXL6, 8q24.3, F-box and leucine-rich repeat protein 6; GPR172A, 8q24.3, G protein-coupled receptor 172A; ADCK5, 8q24.3, aarF domain containing kinase 5; CPSF1, 8q24.3, cleavage and polyadenylation specific factor 1, 160kDa; SLC39A4, ...
... DGAT1, 8q24.3, diacylglycerol O-acyltransferase homolog 1 (mouse); FBXL6, 8q24.3, F-box and leucine-rich repeat protein 6; GPR172A, 8q24.3, G protein-coupled receptor 172A; ADCK5, 8q24.3, aarF domain containing kinase 5; CPSF1, 8q24.3, cleavage and polyadenylation specific factor 1, 160kDa; SLC39A4, ...
DNA research
... The deduced amino acid sequence of the putative ORF of yojP showed strong homology in its C-terminal region with that of B. subtilis NrdE but no homology in its Nterminal region where we could find conserved intein motifs. Eight conserved motifs termed Block A-H have been known in intein sequences.3 ...
... The deduced amino acid sequence of the putative ORF of yojP showed strong homology in its C-terminal region with that of B. subtilis NrdE but no homology in its Nterminal region where we could find conserved intein motifs. Eight conserved motifs termed Block A-H have been known in intein sequences.3 ...
The Interplay of Temperature and Genotype on Patterns
... ABSTRACT Alternative splicing is the highly regulated process of variation in the removal of introns from premessenger-RNA transcripts. The consequences of alternative splicing on the phenotype are well documented, but the impact of the environment on alternative splicing is not yet clear. We studie ...
... ABSTRACT Alternative splicing is the highly regulated process of variation in the removal of introns from premessenger-RNA transcripts. The consequences of alternative splicing on the phenotype are well documented, but the impact of the environment on alternative splicing is not yet clear. We studie ...
Gene cloning of P43 surface protein of toxoplasma gondii tachyzoite
... depends on the stage of the pregnancy period which the infection occurs (Zhao 1992; Wallon et al, 2002). This parasite will be detected in human beings by serological tests only, and specific antigen is very essential in diagnosis system. P43 (SAG3) is one member of the redundant system of T. gondii ...
... depends on the stage of the pregnancy period which the infection occurs (Zhao 1992; Wallon et al, 2002). This parasite will be detected in human beings by serological tests only, and specific antigen is very essential in diagnosis system. P43 (SAG3) is one member of the redundant system of T. gondii ...
LFS_205,_Honors_Biology,_Jana,_Gr._10,_13_pgs
... contrast the structure and function of carbs, proteins, lipids and nucleic acids. ...
... contrast the structure and function of carbs, proteins, lipids and nucleic acids. ...
Genetics
... • Austrian Monk (1850s) • Identified the patterns of inheritance • Laws of Inheritance ...
... • Austrian Monk (1850s) • Identified the patterns of inheritance • Laws of Inheritance ...
genotyping arabidopsis - STLCC.edu :: Users` Server
... concentration of the deoxynucleotides in the reaction is also critical. For example, if the concentration of dNTPs is too high, fidelity of the polymerase (ability to stay attached to the template DNA) will decrease. When the cycle is complete, two double-stranded DNA molecules will result, each con ...
... concentration of the deoxynucleotides in the reaction is also critical. For example, if the concentration of dNTPs is too high, fidelity of the polymerase (ability to stay attached to the template DNA) will decrease. When the cycle is complete, two double-stranded DNA molecules will result, each con ...
Biology Final Exam Review
... 6. The information that directs protein synthesis is contained in DNA's a) sugars. b) phosphate groups. c) nitrogenous bases. d) enzymes. ...
... 6. The information that directs protein synthesis is contained in DNA's a) sugars. b) phosphate groups. c) nitrogenous bases. d) enzymes. ...
Ecology
... 6. Energy loss from one trophic level to the next 7. Define population, community, types of symbiosis (give examples), carrying capacity 8. Define species diversity, where is it at a maximum? Why is it important? 9. Describe major aquatic and terrestrial biomes, know where they occur and what ...
... 6. Energy loss from one trophic level to the next 7. Define population, community, types of symbiosis (give examples), carrying capacity 8. Define species diversity, where is it at a maximum? Why is it important? 9. Describe major aquatic and terrestrial biomes, know where they occur and what ...
Gene Regulation in Development and Evolution
... Nanog—were particularly potent in converting or reprogramming fibroblasts into iPS cells. These reprogrammed cells have most or all of the properties of bona fide ICM cells. The iPS cells can be induced to form just about any cell type, such as cardiomyocytes (heart muscle). In a further remarkable ...
... Nanog—were particularly potent in converting or reprogramming fibroblasts into iPS cells. These reprogrammed cells have most or all of the properties of bona fide ICM cells. The iPS cells can be induced to form just about any cell type, such as cardiomyocytes (heart muscle). In a further remarkable ...
PPT - Biochemistry and Molecular Biology
... a given probe. Wu and Irizarry model the variation in hybridisation of these similar processes using a statistical model. GCRMA determines the contribution to the PM from Signal and from Non-Specific ...
... a given probe. Wu and Irizarry model the variation in hybridisation of these similar processes using a statistical model. GCRMA determines the contribution to the PM from Signal and from Non-Specific ...
GhMYB25-like: a key regulator of early cotton fiber development.
... identical positions in the coding region, but the second intron was considerably larger (1.49 kb compared to 324 bp) in 207D10 than in 241J3 (Genbank Accessions XXX and XXX, respectively). The encoded proteins were 97.8% identical to one another and 69% identical to GhMYB25 (GenBank EU826465). Seque ...
... identical positions in the coding region, but the second intron was considerably larger (1.49 kb compared to 324 bp) in 207D10 than in 241J3 (Genbank Accessions XXX and XXX, respectively). The encoded proteins were 97.8% identical to one another and 69% identical to GhMYB25 (GenBank EU826465). Seque ...
Gene promoters dictate histone occupancy within genes
... Perales et al. in this manuscript investigate the role of the nucleosome assembly factor Spt6 in controlling nucleosome occupancy, particularly in transcribed regions. Previous work had shown that Spt6 contributed to nucleosome re-assembly over coding regions of highly transcribed genes, and that ef ...
... Perales et al. in this manuscript investigate the role of the nucleosome assembly factor Spt6 in controlling nucleosome occupancy, particularly in transcribed regions. Previous work had shown that Spt6 contributed to nucleosome re-assembly over coding regions of highly transcribed genes, and that ef ...
Mitochondrial genes in the colourless alga Prototheca wickerhamii
... intron number is considerable. The gene for subunit I of the cytochrome oxidase (coxl), for example, contains 16 introns in the filamentous fungus Podospora anserina and up to seven introns in various strains of the yeast Saccharomyces cerevisiae, but none in the common laboratory strain of Neurospo ...
... intron number is considerable. The gene for subunit I of the cytochrome oxidase (coxl), for example, contains 16 introns in the filamentous fungus Podospora anserina and up to seven introns in various strains of the yeast Saccharomyces cerevisiae, but none in the common laboratory strain of Neurospo ...
TUTORIAL 8 – DNA - Molecular Movies
... us to add bonds (NURBS cylinders) between atoms generated by the pdbReader script. Another approach will also be to take the spheres generated by the pdbReader script and, instead of adding bonds, we’ll use a MEL script to create a particle at every sphere (jPivToParticle created by Julian Mann) and ...
... us to add bonds (NURBS cylinders) between atoms generated by the pdbReader script. Another approach will also be to take the spheres generated by the pdbReader script and, instead of adding bonds, we’ll use a MEL script to create a particle at every sphere (jPivToParticle created by Julian Mann) and ...
Temperature-sensitive control of protein activity by conditionally
... Inteins have been described as ‘protein introns’ and function posttranslationally to autonomously splice themselves from within a protein context while regenerating an intact host3–6. Temperaturesensitive splicing variants of the Gyr A and recA inteins have been identified7,8 and such conditional in ...
... Inteins have been described as ‘protein introns’ and function posttranslationally to autonomously splice themselves from within a protein context while regenerating an intact host3–6. Temperaturesensitive splicing variants of the Gyr A and recA inteins have been identified7,8 and such conditional in ...
Primary transcript
A primary transcript is the single-stranded ribonucleic acid (RNA) product synthesized by transcription of DNA, and processed to yield various mature RNA products such as mRNAs, tRNAs, and rRNAs. The primary transcripts designated to be mRNAs are modified in preparation for translation. For example, a precursor messenger RNA (pre-mRNA) is a type of primary transcript that becomes a messenger RNA (mRNA) after processing.There are several steps contributing to the production of primary transcripts. All these steps involve a series of interactions to initiate and complete the transcription of DNA in the nucleus of eukaryotes. Certain factors play key roles in the activation and inhibition of transcription, where they regulate primary transcript production. Transcription produces primary transcripts that are further modified by several processes. These processes include the 5' cap, 3'-polyadenylation, and alternative splicing. In particular, alternative splicing directly contributes to the diversity of mRNA found in cells. The modifications of primary transcripts have been further studied in research seeking greater knowledge of the role and significance of these transcripts. Experimental studies based on molecular changes to primary transcripts the processes before and after transcription have led to greater understanding of diseases involving primary transcripts.