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Biology B Trimester Review 6-1
Biology B Trimester Review 6-1

... 22. Identify the 4 different types of nitrogenous bases? 23. Nitrogenous bases can be sorted into two groups. Name the groups and explain how they are classified. 24. What units make up the backbone of DNA? 25. Explain complementary base pairing and the bases involved. 26. What hold base pairs toget ...
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cDNA Library, Human HeLa Cell
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... This cDNA library (plasmid DNA) is constructed from HeLa cell-derived poly(A)+ RNA by the Linker-Primer method (Ref.1) by Professor Hiroshi Nojima of Research Institute for Microbial Diseases, Osaka University. This library is unidirectionally cloned by using the oligo (dT)18 linker primer which con ...
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... and are regulated as a unit. Genes are usually for enzymes that function together in the same pathway. • At the upstream end are sections of DNA that do not code, but rather are binding sites for proteins involved in regulation (turning genes on and off). • The Promoter is the site on DNA recognized ...
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... So how do we get here from there? Or anywhere… • These are a lot of new terms. This language can be bizarre. These concepts could even be a little overwhelming at first. • In a slide or two we will remind you that this can all really be as simple as a process the cells in your body undergo every da ...
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... DNA in thin, non-coiled strands.  DNA is in this form 99% of the time because cells are generally not dividing.  DNA must be in this form in order to be copied (replicated). 2. ________________ - genetic material in _________________ cell’s nucleus; DNA in coiled, rod-shaped form. 1. DNA must be i ...
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... When do chromosomes become visible during the cell cycle? ___________________ As a result of mitosis, a parent cell with 10 chromosomes will produce 2 daughter cells with how many chromosomes? _________ CHAPTER 12: DNA AND RNA 39. Draw and label the parts of a nucleotide for DNA and RNA. ...
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...  a methylated cap at the 5’ end and  about 100–200 adenine nucleotides at the 3’ end. Most eukaryotic genes have regions of base sequences (introns) that are not translated into proteins. Exons contain the actual information for protein formation. The introns are removed, and the mRNA leaves the n ...
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... disease in children caused by a diet high in carbohydrates but lacking in complete protein. When children with kwashiorkor are suddenly put on a diet rich in protein they may become very ill with ammonia poisoning, and some even die. The high level of ammonia in their blood is due to the inadequate ...
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... necessary). In B-DNA the major groove is wide and deep and the minor groove is narrow and deep, whereas in A-DNA the major groove is narrow and deep and the minor groove is wide and shallow. c. In both B-DNA and A-DNA the bases adopt an anti conformation and both DNAs are right-handed. d. The third ...
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... – Ribosome moves along mRNA and new tRNAs come in and line up in order – This brings amino acids in line in a specific order to form a polypeptide – Several ribosomes may move along the same mRNA • Multiple copies of a polypeptide may be made • The entire complex is called a polyribosome ...
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... purple flower, two white alleles (pp) results in a white flower, and one of each allele (Pp) results in a lavender colored flower. Predict the outcome (genotype and phenotype) of crossing one purple and one lavender flower. ...
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... allosteric transition so that the repressor cannot bind the operator DNA anymore. G. A RNA polymerase binds to the promoter and turns on transcription. III. Discovery of the lac system of negative control A. Jacob and Monod found that genes were controlled together. H. Genetic evidence for the prese ...
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FREE Sample Here

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... 26 exons ranging in size from 69 to 3,106 bp, and its 25 introns range in size from 207 to 32,400 bp. The complete gene comprises ~9 kb of exon and ~177 kb of intron. • The biggest human gene yet is for dystrophin. It has >30 exons and is spread over 2.4 million bp. ...
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Replication

... Telomeres are repeats, many thousand times, of a very simple motif. For all chromosomes in all humans (actually, in all vertebrates) the repeating sequence is: 5’TTAGGG3’. Mostly it is dsDNA but at the very end there is a single-stranded 3’ overhang (TTAGGG)n consisting of several dozen repeats. Dur ...
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Biotechnology Cloning of a Gene Cloning a human gene

... • Gene cloning is the engineering and thus production of many identical copies of a genes ...
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Primary transcript



A primary transcript is the single-stranded ribonucleic acid (RNA) product synthesized by transcription of DNA, and processed to yield various mature RNA products such as mRNAs, tRNAs, and rRNAs. The primary transcripts designated to be mRNAs are modified in preparation for translation. For example, a precursor messenger RNA (pre-mRNA) is a type of primary transcript that becomes a messenger RNA (mRNA) after processing.There are several steps contributing to the production of primary transcripts. All these steps involve a series of interactions to initiate and complete the transcription of DNA in the nucleus of eukaryotes. Certain factors play key roles in the activation and inhibition of transcription, where they regulate primary transcript production. Transcription produces primary transcripts that are further modified by several processes. These processes include the 5' cap, 3'-polyadenylation, and alternative splicing. In particular, alternative splicing directly contributes to the diversity of mRNA found in cells. The modifications of primary transcripts have been further studied in research seeking greater knowledge of the role and significance of these transcripts. Experimental studies based on molecular changes to primary transcripts the processes before and after transcription have led to greater understanding of diseases involving primary transcripts.
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