Developmental timing in Dictyostelium is regulated by the Set1 histone methyltransferase
... genome (Eichinger et al., 2005) that encodes chromatin proteins and modifying enzymes conserved with those in higher eukaryotes. In addition, the organism is highly amenable, with powerful haploid molecular genetics (De Lozanne and Spudich, 1987). Individual Dictyostelium cells enter their program o ...
... genome (Eichinger et al., 2005) that encodes chromatin proteins and modifying enzymes conserved with those in higher eukaryotes. In addition, the organism is highly amenable, with powerful haploid molecular genetics (De Lozanne and Spudich, 1987). Individual Dictyostelium cells enter their program o ...
Efficiency of gene silencing in Arabidopsis
... and homologous inverted repeat (hIR) vectors. hIR constructs carry self-complementary intron-spliced fragments of the target gene whereas transitive vectors have the target sequence fragment adjacent to an intron-spliced, inverted repeat of heterologous origin. Both transitive and hIR constructs fac ...
... and homologous inverted repeat (hIR) vectors. hIR constructs carry self-complementary intron-spliced fragments of the target gene whereas transitive vectors have the target sequence fragment adjacent to an intron-spliced, inverted repeat of heterologous origin. Both transitive and hIR constructs fac ...
genetics - Your Heading Goes Here
... 1. Describe the basic structure of all cells and distinguish between prokaryotic and eukaryotic cells B. Heritable information (7-8, 86-89) 1. Explain the basic structure of DNA and how it encodes information to build proteins. Use a specific example. 2. If a DNA strand has the sequence A-G-T-C-C-T, ...
... 1. Describe the basic structure of all cells and distinguish between prokaryotic and eukaryotic cells B. Heritable information (7-8, 86-89) 1. Explain the basic structure of DNA and how it encodes information to build proteins. Use a specific example. 2. If a DNA strand has the sequence A-G-T-C-C-T, ...
Identification of lineage-specific zygotic transcripts in early
... including the 3Vregion of the genes (see below) and washed (Church and Gilbert, 1984) prior to exposure to autoradiographic film (Kodak). DNA fragments used to analyze individual 4- and 12-cell embryo cDNAs, all derived from cDNA, are tba-1, 1037 bp including 136 bp 3VUTR; cdk-1, 859 bp including 55 ...
... including the 3Vregion of the genes (see below) and washed (Church and Gilbert, 1984) prior to exposure to autoradiographic film (Kodak). DNA fragments used to analyze individual 4- and 12-cell embryo cDNAs, all derived from cDNA, are tba-1, 1037 bp including 136 bp 3VUTR; cdk-1, 859 bp including 55 ...
Fluorescent - Nanoimaging - Friedrich-Schiller
... neutralize foreign substances (antigens) Antibodies are made of two large heavy chains (~440 amino acids) and two small light chains (~220 amino acids) C-region is similar for all antibodies, while the V-region is extremely variable and forms the specific binding site for the antigen i.e. every ...
... neutralize foreign substances (antigens) Antibodies are made of two large heavy chains (~440 amino acids) and two small light chains (~220 amino acids) C-region is similar for all antibodies, while the V-region is extremely variable and forms the specific binding site for the antigen i.e. every ...
Biomarker for Spinal Muscular Atrophy: Expression
... could not be determined for every patient since we were limited by the blood volume allowed to be drawn from children. In two cases the copy number reported by the patients did not match the copy number determined by the digital PCR assay. For these cases we used the digital PCR values. The healthy ...
... could not be determined for every patient since we were limited by the blood volume allowed to be drawn from children. In two cases the copy number reported by the patients did not match the copy number determined by the digital PCR assay. For these cases we used the digital PCR values. The healthy ...
2 - cellbiochem.ca
... • Antibiotic resistance gene: allow for selection for bacterial cells that have taken up the vector ...
... • Antibiotic resistance gene: allow for selection for bacterial cells that have taken up the vector ...
Gene prediction and Genome Annotation
... • New genomes require new approaches or at least require re-optimilisation of software • Isoforms (NGS data) • “New genes” , eg. lncRNAs, anti-sense RNAs, … • New “organisation” of genes – Overlapping genes (transcript, CDS) – Nested genes ...
... • New genomes require new approaches or at least require re-optimilisation of software • Isoforms (NGS data) • “New genes” , eg. lncRNAs, anti-sense RNAs, … • New “organisation” of genes – Overlapping genes (transcript, CDS) – Nested genes ...
Characterizing a Lambda Red Recombinase Induced Presumptive
... manipulation was not needed. IPTG is an inducer of the lac operon, that promotes the production of β-galactosidase (2). IPTG is a common substance used to increase β-galactosidase in absence of lactose. In practice, it is beneficial to have an E.coli strain that constantly expresses βgalactosidase, ...
... manipulation was not needed. IPTG is an inducer of the lac operon, that promotes the production of β-galactosidase (2). IPTG is a common substance used to increase β-galactosidase in absence of lactose. In practice, it is beneficial to have an E.coli strain that constantly expresses βgalactosidase, ...
informe tecnológico de patentes
... effective therapy. However, if reduction of wild-type protein is not well tolerated in the brain, it may be necessary to suppress just the product of the mutant allele. We present a small interfering RNA (siRNA) that selectively reduces the endogenous mRNA for a heterozygous HD donor's pathogenic al ...
... effective therapy. However, if reduction of wild-type protein is not well tolerated in the brain, it may be necessary to suppress just the product of the mutant allele. We present a small interfering RNA (siRNA) that selectively reduces the endogenous mRNA for a heterozygous HD donor's pathogenic al ...
Course Objectives
... 5. Student describes the structure and function of the macromolecules important to organisms, with emphasis on carbohydrates, lipids, proteins, and nucleic acids. 6. Student explains the structure and function of the cellular components. 7. Student describes energy transfer within cells and the role ...
... 5. Student describes the structure and function of the macromolecules important to organisms, with emphasis on carbohydrates, lipids, proteins, and nucleic acids. 6. Student explains the structure and function of the cellular components. 7. Student describes energy transfer within cells and the role ...
Aus dem Max-Planck-Institut für Psychiatrie
... As fellow mammals, mice and humans possess a similar anatomy and physiology. Furthermore, genomic analysis indicates that a mouse gene equivalent, an orthologue, exists for about 99,5% of human disease genes, amongst which the ones with neurological function exhibit the highest grade of evolutionary ...
... As fellow mammals, mice and humans possess a similar anatomy and physiology. Furthermore, genomic analysis indicates that a mouse gene equivalent, an orthologue, exists for about 99,5% of human disease genes, amongst which the ones with neurological function exhibit the highest grade of evolutionary ...
DNA methylation controls histone H3 lysine 9 methylation
... 2001; Lachner and Jenuwein, 2002; Richards and Elgin, 2002). The interactions between DNA methylation, histone modi®cations and chromatin structure have mainly been studied at the molecular level for speci®c DNA sequences. Integrated genetic, molecular and cytological approaches can provide new insi ...
... 2001; Lachner and Jenuwein, 2002; Richards and Elgin, 2002). The interactions between DNA methylation, histone modi®cations and chromatin structure have mainly been studied at the molecular level for speci®c DNA sequences. Integrated genetic, molecular and cytological approaches can provide new insi ...
Thesis-1965R-K29r
... of the nucleolus with the mitotic cycle and cytoplasm was also obtained. Pub I ications since the year 1959 were consulted. The materials used were chiefly periodicals and a few books. Findings and Conclusions: Progress of research on the nucleolus has awaited improved techniques and new types of eq ...
... of the nucleolus with the mitotic cycle and cytoplasm was also obtained. Pub I ications since the year 1959 were consulted. The materials used were chiefly periodicals and a few books. Findings and Conclusions: Progress of research on the nucleolus has awaited improved techniques and new types of eq ...
Illustrating Python via Bioinformatics Examples
... The instructions to the computer how the analysis is going to be performed are specified using the Python1 programming language. The forthcoming examples are simple illustrations of the type of problem settings and corresponding Python implementations that are encountered in bioinformatics. However, ...
... The instructions to the computer how the analysis is going to be performed are specified using the Python1 programming language. The forthcoming examples are simple illustrations of the type of problem settings and corresponding Python implementations that are encountered in bioinformatics. However, ...
Journal of Plant Physiology
... protein (AP2/EREBP) transcription factors have important functions in the transcriptional regulation of biological processes related to growth and development, as well as in responses to environmental stimuli (Riechmann and Meyerowitz, 1998; Gutterson and Reuber, 2004). The Arabidopsis genome encode ...
... protein (AP2/EREBP) transcription factors have important functions in the transcriptional regulation of biological processes related to growth and development, as well as in responses to environmental stimuli (Riechmann and Meyerowitz, 1998; Gutterson and Reuber, 2004). The Arabidopsis genome encode ...
Iron-sulfur Clusters in RNA Polymerase: A New Role for an Ancient
... Department of Biological Sciences, University of Arkansas, Fayetteville, AR 72701; 2Arkansas Center for Space and Planetary Sciences, University of Arkansas, Fayetteville, AR 72701; 3Department of Botany and Microbiology, University of Oklahoma, Norman, OK 73019 Introduction Iron-sulfur (Fe-S) clust ...
... Department of Biological Sciences, University of Arkansas, Fayetteville, AR 72701; 2Arkansas Center for Space and Planetary Sciences, University of Arkansas, Fayetteville, AR 72701; 3Department of Botany and Microbiology, University of Oklahoma, Norman, OK 73019 Introduction Iron-sulfur (Fe-S) clust ...
Trawling DNA Databases For Partial Matches: What Is The FBI
... DNA evidence is often presented as the “gold standard” for forensic science. But this was not always the case. For years, eminent scientists complained that the estimates of the tiny frequencies of DNA types were unfounded. It took scores of research papers, dozens of judicial opinions, and two comm ...
... DNA evidence is often presented as the “gold standard” for forensic science. But this was not always the case. For years, eminent scientists complained that the estimates of the tiny frequencies of DNA types were unfounded. It took scores of research papers, dozens of judicial opinions, and two comm ...
DNA How the Molecule of Heredity Carries, Replicates, and
... DNA stores information in the sequence of its bases. •Much of DNA’s sequence-specific information is accessible only when the double helix is unwound. •Proteins read the DNA sequence of nucleotides as the DNA helix unwinds. Proteins can either bind to a DNA sequence, or initiate the copying of it. ...
... DNA stores information in the sequence of its bases. •Much of DNA’s sequence-specific information is accessible only when the double helix is unwound. •Proteins read the DNA sequence of nucleotides as the DNA helix unwinds. Proteins can either bind to a DNA sequence, or initiate the copying of it. ...
The tightly regulated promoter of the xanA gene of
... we have shown that the one from Neurospora crassa fully complements a xanA deletion (Cultrone et al., 2005). In this article we investigate whether this gene is subject to the same regulatory signals as all other enzymes of the purine degradation pathway. We observed that the promoter element of xan ...
... we have shown that the one from Neurospora crassa fully complements a xanA deletion (Cultrone et al., 2005). In this article we investigate whether this gene is subject to the same regulatory signals as all other enzymes of the purine degradation pathway. We observed that the promoter element of xan ...
ARGONAUTE1 Acts in Arabidopsis Root Radial
... miRNAs and their target genes (Rajagopalan et al. 2006, Fahlgren et al. 2007). Processed from imperfectly complementary stem–loop precursor RNAs (Bartel 2004), miRNAs regulate gene expression by targeting their complementary mRNA for cleavage or translational inhibition (Tang et al. 2003, Chen 2004) ...
... miRNAs and their target genes (Rajagopalan et al. 2006, Fahlgren et al. 2007). Processed from imperfectly complementary stem–loop precursor RNAs (Bartel 2004), miRNAs regulate gene expression by targeting their complementary mRNA for cleavage or translational inhibition (Tang et al. 2003, Chen 2004) ...
Amplification of 16S rRNA Genes from Frankia Strains in Root
... Root nodules. The sources of the root nodules used in this study are listed in Table 1. In all cases the nodules were frozen at 2258C after collection or receipt at the University of Waikato. DNA isolation. Extreme care was used to avoid exogenous DNA in all solutions and during isolation of Frankia ...
... Root nodules. The sources of the root nodules used in this study are listed in Table 1. In all cases the nodules were frozen at 2258C after collection or receipt at the University of Waikato. DNA isolation. Extreme care was used to avoid exogenous DNA in all solutions and during isolation of Frankia ...
Primary transcript
A primary transcript is the single-stranded ribonucleic acid (RNA) product synthesized by transcription of DNA, and processed to yield various mature RNA products such as mRNAs, tRNAs, and rRNAs. The primary transcripts designated to be mRNAs are modified in preparation for translation. For example, a precursor messenger RNA (pre-mRNA) is a type of primary transcript that becomes a messenger RNA (mRNA) after processing.There are several steps contributing to the production of primary transcripts. All these steps involve a series of interactions to initiate and complete the transcription of DNA in the nucleus of eukaryotes. Certain factors play key roles in the activation and inhibition of transcription, where they regulate primary transcript production. Transcription produces primary transcripts that are further modified by several processes. These processes include the 5' cap, 3'-polyadenylation, and alternative splicing. In particular, alternative splicing directly contributes to the diversity of mRNA found in cells. The modifications of primary transcripts have been further studied in research seeking greater knowledge of the role and significance of these transcripts. Experimental studies based on molecular changes to primary transcripts the processes before and after transcription have led to greater understanding of diseases involving primary transcripts.