Leukaemia Section t(11;17)(q23;q21) Atlas of Genetics and Cytogenetics in Oncology and Haematology
... 1- As a result of the alternative splicing of PLZF gene, two forms of PLZF-RARa protein can be detected: a) PLZF(A)-RARa (735 amino acids; 81 kDa) composed of the N-term part of PLZF including POZ domain and two of the nine zinc fingers, fused to the DNA and ligand binding domains of RARa. b) PLZF(B ...
... 1- As a result of the alternative splicing of PLZF gene, two forms of PLZF-RARa protein can be detected: a) PLZF(A)-RARa (735 amino acids; 81 kDa) composed of the N-term part of PLZF including POZ domain and two of the nine zinc fingers, fused to the DNA and ligand binding domains of RARa. b) PLZF(B ...
A framework for describing genetic diseases
... new mutations in gametes (usually point mutations, and usually in sperm from fathers age 35 and older). The predominance of spontaneous mutation in achondroplasia stems from the fact that this disease inhibits reproductive success and that the gene involved has a mutation rate higher than the averag ...
... new mutations in gametes (usually point mutations, and usually in sperm from fathers age 35 and older). The predominance of spontaneous mutation in achondroplasia stems from the fact that this disease inhibits reproductive success and that the gene involved has a mutation rate higher than the averag ...
- Wiley Online Library
... functionality and application of the sensitive SplitGFP (split green fluorescent protein) detection system in insect cells. The successful expression of constructs is monitored by direct measurement of the fluorescence in the BioLector Microcultivation system. Additionally, we show that the results ob ...
... functionality and application of the sensitive SplitGFP (split green fluorescent protein) detection system in insect cells. The successful expression of constructs is monitored by direct measurement of the fluorescence in the BioLector Microcultivation system. Additionally, we show that the results ob ...
The Agrozobium™ project
... Sections of rice root stained yellow-green by activity of GUSPlusTM using ELF-97-GlcA substrate. • GRP signal peptide directs GUSPlusTM to cell wall apoplastic space more efficiently than extensin signal peptide ...
... Sections of rice root stained yellow-green by activity of GUSPlusTM using ELF-97-GlcA substrate. • GRP signal peptide directs GUSPlusTM to cell wall apoplastic space more efficiently than extensin signal peptide ...
Bioinformatics Resources at a Glance A Note about FASTA Format
... at the top of the screen. Since there are variations among species, be sure to select the protein from the appropriate species. 6. Click on the link to the protein you want to explore. This will bring up resource page in the NCBI – this is a GREAT place to get links to journal articles related to ...
... at the top of the screen. Since there are variations among species, be sure to select the protein from the appropriate species. 6. Click on the link to the protein you want to explore. This will bring up resource page in the NCBI – this is a GREAT place to get links to journal articles related to ...
Optimizing bacterial expression and purifica- Biomedical laboratory science,
... Despite the discovery of deoxyribonucleic acid (DNA) in the early 1950’s and the subsequent discovery of the structure of DNA by the 1960’s, it was only during the 1970’s, when a new methodology called recombinant DNA technology or genetic engineering was developed. This new methodology made it poss ...
... Despite the discovery of deoxyribonucleic acid (DNA) in the early 1950’s and the subsequent discovery of the structure of DNA by the 1960’s, it was only during the 1970’s, when a new methodology called recombinant DNA technology or genetic engineering was developed. This new methodology made it poss ...
8. Peptide bonds, polypeptides and proteins Polypeptide and
... What these variations in the genetic code illustrate is that evolutionary mechanisms can change the genetic code.221 Since the genetic code does not appear to be predetermined, the general conservation of the genetic code among organisms is seen as strong evidence that all organisms (even the ones w ...
... What these variations in the genetic code illustrate is that evolutionary mechanisms can change the genetic code.221 Since the genetic code does not appear to be predetermined, the general conservation of the genetic code among organisms is seen as strong evidence that all organisms (even the ones w ...
Leukaemia Section del(11)(q23q23) MLL/CBL t(11;11)(q23;q23) MLL/CBL Atlas of Genetics and Cytogenetics
... may acetylates H3 and H4 in the HOX area; a SET domain: methyltransferase; methylates H3, including histones in the HOX area for allowing chromatin to be open to transcription. MLL is cleaved by taspase 1 into 2 proteins before entering the nucleus: a p300/320 N-term protein called MLL-N, and a p180 ...
... may acetylates H3 and H4 in the HOX area; a SET domain: methyltransferase; methylates H3, including histones in the HOX area for allowing chromatin to be open to transcription. MLL is cleaved by taspase 1 into 2 proteins before entering the nucleus: a p300/320 N-term protein called MLL-N, and a p180 ...
DNA and Protein Concentration Measurements Using Fluorescence
... the Broad Range DNA Assay Kit. Using this kit, the Curie was able to detect dsDNA to 5 mg/mL concentration at a 1-s integration time. Figure 3 shows the fluorescence spectra acquired for the Protein Assay Kit. Using this kit, the Curie was able to detect protein concentration to 25 mg/mL at a 0.5-s ...
... the Broad Range DNA Assay Kit. Using this kit, the Curie was able to detect dsDNA to 5 mg/mL concentration at a 1-s integration time. Figure 3 shows the fluorescence spectra acquired for the Protein Assay Kit. Using this kit, the Curie was able to detect protein concentration to 25 mg/mL at a 0.5-s ...
Document
... the molecules in a material. Increasing the temperature increases the translational motion of molecules. ...
... the molecules in a material. Increasing the temperature increases the translational motion of molecules. ...
PowerPoint-Präsentation
... noise“(variations of other cell components such as RNA polymerase) Idea for an experiment: Gene for CFP (green fluorescent protein) und YFP (yellow fluorescent protein, shown in red) are controlled by the same promotor, hence the mean concentration of CFP and YFP is equal => Expression probability s ...
... noise“(variations of other cell components such as RNA polymerase) Idea for an experiment: Gene for CFP (green fluorescent protein) und YFP (yellow fluorescent protein, shown in red) are controlled by the same promotor, hence the mean concentration of CFP and YFP is equal => Expression probability s ...
Normalization between a pair of arrays
... Some regulatory proteins play targeted role i.e. they take part in regulation of a few genes. Some regulatory proteins play more general role in initiating transcription (for example the eukaryotic transcription factors of type II or the RNA polymerase itself that is essential for the transcription ...
... Some regulatory proteins play targeted role i.e. they take part in regulation of a few genes. Some regulatory proteins play more general role in initiating transcription (for example the eukaryotic transcription factors of type II or the RNA polymerase itself that is essential for the transcription ...
Biochemistry 304 2014 Student Edition Membranes
... of molecules across that boundary. “Compartmentalization” In eukaryotic cells they further define the cell into internal compartments with discrete functions and components. They organize complex reaction sequences and are key to biological energy conservation and communication between cells. The bi ...
... of molecules across that boundary. “Compartmentalization” In eukaryotic cells they further define the cell into internal compartments with discrete functions and components. They organize complex reaction sequences and are key to biological energy conservation and communication between cells. The bi ...
Intrinsically Disordered Proteins (IDPs)
... • Whole proteins and regions of proteins are intrinsically disordered if they lack stable 3D structure under physiological conditions, • But exist instead as highly dynamic, rapidly interconverting ensembles without particular equilibrium values for their coordinates or bond angles and with noncoope ...
... • Whole proteins and regions of proteins are intrinsically disordered if they lack stable 3D structure under physiological conditions, • But exist instead as highly dynamic, rapidly interconverting ensembles without particular equilibrium values for their coordinates or bond angles and with noncoope ...
Outline for Lecture #5
... III. What do Results of Measurement Look Like? What do they mean? A. Curve # 1 -- Uptake of X vs time: Measure [X] in at increasing times at some starting, outside concentration of X; plot conc. of X inside vs. time. Curve always levels off -- but at what value? This allows you to distinguish a ...
... III. What do Results of Measurement Look Like? What do they mean? A. Curve # 1 -- Uptake of X vs time: Measure [X] in at increasing times at some starting, outside concentration of X; plot conc. of X inside vs. time. Curve always levels off -- but at what value? This allows you to distinguish a ...
Rapid Translation System RTS 500 E. coli HY Kit
... Cell-free protein expression has a strong potential to meet these requirements because handling of intact cells (e.g., transformation, fermentation, lysis) is not necessary. In fact, this methodology only involves liquid handling and can thus be adapted to automated processes. Low yield has been the ...
... Cell-free protein expression has a strong potential to meet these requirements because handling of intact cells (e.g., transformation, fermentation, lysis) is not necessary. In fact, this methodology only involves liquid handling and can thus be adapted to automated processes. Low yield has been the ...
Severe Perinatal Thrombosis in Double and Triple Heterozygous
... for the father (III.2) (Table 1) were not useful because this individual had received anticoagulant therapy as he initially presented with clinical symptoms. His father (11.2) exhibited both protein C and protein S deficiency phenotypes (types I1 and 111, respectively) (Table l). Although matemal (1 ...
... for the father (III.2) (Table 1) were not useful because this individual had received anticoagulant therapy as he initially presented with clinical symptoms. His father (11.2) exhibited both protein C and protein S deficiency phenotypes (types I1 and 111, respectively) (Table l). Although matemal (1 ...
OSIRIS: a tool for retrieving literature about sequence variants
... dbSNP accession number (rs code), the type of variation (snp, in-del, etc.), its position in the sequence and the alternative alleles are given. Finally, the list of articles found for each variant, and a list of articles that result from a ‘generic’ search are provided (see the Methods section for ...
... dbSNP accession number (rs code), the type of variation (snp, in-del, etc.), its position in the sequence and the alternative alleles are given. Finally, the list of articles found for each variant, and a list of articles that result from a ‘generic’ search are provided (see the Methods section for ...
Agrobacterium tumefaciens and A. rhizogenes use different proteins
... tissue. Pathogenesis results when transformed plant cells express rol (root loci) genes transferred from the rootinducing (Ri) plasmid (White et al., 1985). In contrast, A. tumefaciens causes unorganized growth of infected plant cells. Oncogenes transferred from the tumour-inducing (Ti) plasmid into ...
... tissue. Pathogenesis results when transformed plant cells express rol (root loci) genes transferred from the rootinducing (Ri) plasmid (White et al., 1985). In contrast, A. tumefaciens causes unorganized growth of infected plant cells. Oncogenes transferred from the tumour-inducing (Ti) plasmid into ...
Testing for Lipids, Proteins and Carbs
... composed of carbon, oxygen, hydrogen, nitrogen and sometimes sulfur. The building blocks of proteins are amino acids. There are 20 different amino acids that combine to form polypeptides (proteins). The different amino acids are similar in structure: at the center of the molecule is the alpha carbon ...
... composed of carbon, oxygen, hydrogen, nitrogen and sometimes sulfur. The building blocks of proteins are amino acids. There are 20 different amino acids that combine to form polypeptides (proteins). The different amino acids are similar in structure: at the center of the molecule is the alpha carbon ...
Document
... The required signal sequence for a protein to enter the ER is 15– 30 N-terminal amino acids. As the signal sequence is produced by translation, it is bound by a signal recognition particle (SRP) composed of RNA and protein. The SRP suspends translation until the complex binds a docking protein on th ...
... The required signal sequence for a protein to enter the ER is 15– 30 N-terminal amino acids. As the signal sequence is produced by translation, it is bound by a signal recognition particle (SRP) composed of RNA and protein. The SRP suspends translation until the complex binds a docking protein on th ...
Introduction 1.1 The Importance of Homeostasis in Maintaining
... number of complex processes and events detailing the whole response. The phosphorylation state of the heat shock transcription factor in S. cerevisiae (ScHsf1) is found in the nucleus bound to HSE under normal conditions (Pelham & Jakobsen, 1988). Two transcriptional domains namely AR1 and AR2 locat ...
... number of complex processes and events detailing the whole response. The phosphorylation state of the heat shock transcription factor in S. cerevisiae (ScHsf1) is found in the nucleus bound to HSE under normal conditions (Pelham & Jakobsen, 1988). Two transcriptional domains namely AR1 and AR2 locat ...
Cell Death and Differentiation
... ApoCaM differs from Ca2 þ -bound CaM in its three-dimensional structure, and it also binds target proteins differently, utilizing binding motifs such as the IQ motif (IQXXXRGXXXR) and noncontiguous binding sites.9,10 Therefore, CaM may cycle between Ca2 þ -free and Ca2 þ -bound states, and bind diff ...
... ApoCaM differs from Ca2 þ -bound CaM in its three-dimensional structure, and it also binds target proteins differently, utilizing binding motifs such as the IQ motif (IQXXXRGXXXR) and noncontiguous binding sites.9,10 Therefore, CaM may cycle between Ca2 þ -free and Ca2 þ -bound states, and bind diff ...
to the complete text
... proteins. Within this subset, 55 ORFs that encode proteins containing only one additional hydrophobic domain at the extreme C-terminus were found. In this set it was determined whether a potential GPI-attachment signal could be found according to the consensus rules described by Nuoffer et al. (1993 ...
... proteins. Within this subset, 55 ORFs that encode proteins containing only one additional hydrophobic domain at the extreme C-terminus were found. In this set it was determined whether a potential GPI-attachment signal could be found according to the consensus rules described by Nuoffer et al. (1993 ...
Plants have evolved multiple defense mechanisms against
... viruses that interfere with the infection process at several key stages. Potato plants carrying Rx, which encodes CC-NB-LRR type R protein, establish an extreme resistance (ER) to potato virus X (PVX) and severely attenuate virus multiplication. In resistant tobacco plants, tobacco mosaic virus (TMV ...
... viruses that interfere with the infection process at several key stages. Potato plants carrying Rx, which encodes CC-NB-LRR type R protein, establish an extreme resistance (ER) to potato virus X (PVX) and severely attenuate virus multiplication. In resistant tobacco plants, tobacco mosaic virus (TMV ...
Protein moonlighting
Protein moonlighting (or gene sharing) is a phenomenon by which a protein can perform more than one function. Ancestral moonlighting proteins originally possessed a single function but through evolution, acquired additional functions. Many proteins that moonlight are enzymes; others are receptors, ion channels or chaperones. The most common primary function of moonlighting proteins is enzymatic catalysis, but these enzymes have acquired secondary non-enzymatic roles. Some examples of functions of moonlighting proteins secondary to catalysis include signal transduction, transcriptional regulation, apoptosis, motility, and structural.Protein moonlighting may occur widely in nature. Protein moonlighting through gene sharing differs from the use of a single gene to generate different proteins by alternative RNA splicing, DNA rearrangement, or post-translational processing. It is also different from multifunctionality of the protein, in which the protein has multiple domains, each serving a different function. Protein moonlighting by gene sharing means that a gene may acquire and maintain a second function without gene duplication and without loss of the primary function. Such genes are under two or more entirely different selective constraints.Various techniques have been used to reveal moonlighting functions in proteins. The detection of a protein in unexpected locations within cells, cell types, or tissues may suggest that a protein has a moonlighting function. Furthermore, sequence or structure homology of a protein may be used to infer both primary function as well as secondary moonlighting functions of a protein.The most well-studied examples of gene sharing are crystallins. These proteins, when expressed at low levels in many tissues function as enzymes, but when expressed at high levels in eye tissue, become densely packed and thus form lenses. While the recognition of gene sharing is relatively recent—the term was coined in 1988, after crystallins in chickens and ducks were found to be identical to separately identified enzymes—recent studies have found many examples throughout the living world. Joram Piatigorsky has suggested that many or all proteins exhibit gene sharing to some extent, and that gene sharing is a key aspect of molecular evolution. The genes encoding crystallins must maintain sequences for catalytic function and transparency maintenance function.Inappropriate moonlighting is a contributing factor in some genetic diseases, and moonlighting provides a possible mechanism by which bacteria may become resistant to antibiotics.