Chemical Genetics: Drug Screens in Zebrafish
... screening for small molecule inhibitors of diseases. The Zebrafish Models System is Amenable to Large Scale Analyses The zebrafish is emerging as a versatile model system for genetic and molecular analyses. The unique features of zebrafish, including the transparency of their embryos, development outsi ...
... screening for small molecule inhibitors of diseases. The Zebrafish Models System is Amenable to Large Scale Analyses The zebrafish is emerging as a versatile model system for genetic and molecular analyses. The unique features of zebrafish, including the transparency of their embryos, development outsi ...
Figure S1. Chloroplast localization and topology of TerC
... thylakoid membranes of transformed Arabidopsis lines. Salt treatments were performed as described previously (Armbruster et al. 2010) using antibodies specific for GFP, Lhcb1 (Agrisera) and PsaD (Agrisera). For thermolysin treatments, isolated thylakoid membranes were resuspended in10 mM HEPES-KOH, ...
... thylakoid membranes of transformed Arabidopsis lines. Salt treatments were performed as described previously (Armbruster et al. 2010) using antibodies specific for GFP, Lhcb1 (Agrisera) and PsaD (Agrisera). For thermolysin treatments, isolated thylakoid membranes were resuspended in10 mM HEPES-KOH, ...
AUGUSTUS: a web server for gene prediction in eukaryotes that
... a probabilistic model of a sequence and its gene structure. The web server allows the user to impose constraints on the predicted gene structure. A constraint can specify the position of a splice site, a translation initiation site or a stop codon. Furthermore, it is possible to specify the position ...
... a probabilistic model of a sequence and its gene structure. The web server allows the user to impose constraints on the predicted gene structure. A constraint can specify the position of a splice site, a translation initiation site or a stop codon. Furthermore, it is possible to specify the position ...
PCT/MIA/8/2 ADD.2
... to reasons other than prior art, i.e. requirements under the Treaty, such as sufficient support by the description or industrial applicability.” Because this proposal follows the plain language of PCT Rule 13 and simply represents a more expansive determination of exactly what constitutes a “contrib ...
... to reasons other than prior art, i.e. requirements under the Treaty, such as sufficient support by the description or industrial applicability.” Because this proposal follows the plain language of PCT Rule 13 and simply represents a more expansive determination of exactly what constitutes a “contrib ...
Chlamydia effector proteins and new insights into chlamydial
... Chlamydiae have a very complex infectious cycle. Infection begins with the attachment of an elementary body (EB), a metabolically inactive ‘spore-like’ form of the bacteria, to the surface of epithelial cells (Figure 1). After attachment, C. trachomatis induces the localized activation of the Rho-GT ...
... Chlamydiae have a very complex infectious cycle. Infection begins with the attachment of an elementary body (EB), a metabolically inactive ‘spore-like’ form of the bacteria, to the surface of epithelial cells (Figure 1). After attachment, C. trachomatis induces the localized activation of the Rho-GT ...
Gene7-10
... binding to a regulator protein. Gratuitous inducers resemble authentic inducers of transcription but are not substrates for the induced enzymes. Inducer is a small molecule that triggers gene transcription by binding to a regulator protein. Induction refers to the ability of bacteria (or yeast) to s ...
... binding to a regulator protein. Gratuitous inducers resemble authentic inducers of transcription but are not substrates for the induced enzymes. Inducer is a small molecule that triggers gene transcription by binding to a regulator protein. Induction refers to the ability of bacteria (or yeast) to s ...
Architecture of the trypanosome RNA editing accessory complex
... exceptions) minicircle-encoded guide RNAs (gRNAs). RNA editing is catalyzed by the multiprotein RNA editing core complex (RECC), also known as the editosome. Pre-mRNA and cognate gRNA form an anchor duplex, with the sites to be edited located upstream of the anchor duplex. The central region of the ...
... exceptions) minicircle-encoded guide RNAs (gRNAs). RNA editing is catalyzed by the multiprotein RNA editing core complex (RECC), also known as the editosome. Pre-mRNA and cognate gRNA form an anchor duplex, with the sites to be edited located upstream of the anchor duplex. The central region of the ...
Genomic Annotation Lab Exercise By Jacob Jipp and Marian
... sequence. A variety of queries can be used which enables sequence similarity to be identified at the protein and nucleotide level. Based on this sequence similarity, speculations can be made as to the homology of two genes. Evolutionarily speaking, these similarities can be interpreted as divergent ...
... sequence. A variety of queries can be used which enables sequence similarity to be identified at the protein and nucleotide level. Based on this sequence similarity, speculations can be made as to the homology of two genes. Evolutionarily speaking, these similarities can be interpreted as divergent ...
Genomic Annotation Lab Exercise By Jacob Jipp and Marian
... sequence. A variety of queries can be used which enables sequence similarity to be identified at the protein and nucleotide level. Based on this sequence similarity, speculations can be made as to the homology of two genes. Evolutionarily speaking, these similarities can be interpreted as divergent ...
... sequence. A variety of queries can be used which enables sequence similarity to be identified at the protein and nucleotide level. Based on this sequence similarity, speculations can be made as to the homology of two genes. Evolutionarily speaking, these similarities can be interpreted as divergent ...
Modified `one amino acid-one codon` engineering of high GC
... linked to thermophily [1,6]. Differences in codon usage between species adversely affect recombinant gene expression levels, thus gene optimization is often needed to obtain adequate expression levels, which is especially important for industrial enzyme production processes. Natural REase-coding gen ...
... linked to thermophily [1,6]. Differences in codon usage between species adversely affect recombinant gene expression levels, thus gene optimization is often needed to obtain adequate expression levels, which is especially important for industrial enzyme production processes. Natural REase-coding gen ...
Lysine Acetylation - Regulator of Diverse Cellular Processes
... Lysine Acetylation - Regulator of Diverse Cellular Processes Lysine acetylation is a post-translational modification (PTM) crucial for regulating the function and localization of many eukaryotic proteins. This PTM is reversible, regulated by histone deacetylases (HDACs) and histone acetyltransferase ...
... Lysine Acetylation - Regulator of Diverse Cellular Processes Lysine acetylation is a post-translational modification (PTM) crucial for regulating the function and localization of many eukaryotic proteins. This PTM is reversible, regulated by histone deacetylases (HDACs) and histone acetyltransferase ...
source file
... Kyoto Encyclopedia of Genes and Genomes - KEGG is a collection of biological information compiled from published material curated database. - Includes information on genes, proteins, metabolic pathways, molecular interactions, and biochemical reactions associated with specific organisms - Provide ...
... Kyoto Encyclopedia of Genes and Genomes - KEGG is a collection of biological information compiled from published material curated database. - Includes information on genes, proteins, metabolic pathways, molecular interactions, and biochemical reactions associated with specific organisms - Provide ...
Leukaemia Section t(10;12)(q24;p13) Atlas of Genetics and Cytogenetics in Oncology and Haematology
... Two functional domains have been indentified: a Nterminal Helix-Loop-Helix domain (or pointed (PNT) or Sterile Alpha Motif (SAM) domain) responsible for hetero- and homodimerization with itself and possibly other proteins, and a C-terminal ETS domain responsible for a specific DNA binding. HLH domai ...
... Two functional domains have been indentified: a Nterminal Helix-Loop-Helix domain (or pointed (PNT) or Sterile Alpha Motif (SAM) domain) responsible for hetero- and homodimerization with itself and possibly other proteins, and a C-terminal ETS domain responsible for a specific DNA binding. HLH domai ...
Analysis of a Rhizobium leguminosarum gene
... enzymes of higher plants. The Rhizobium gstA gene was normally expressed at a very low level. The product of gstA was over-expressed and purified from Escherichia coli. It was shown to bind to the affinity matrix glutathioneSepharose, but no enzymic GST activity with l-chloro-2,4-dinitrobenzene as s ...
... enzymes of higher plants. The Rhizobium gstA gene was normally expressed at a very low level. The product of gstA was over-expressed and purified from Escherichia coli. It was shown to bind to the affinity matrix glutathioneSepharose, but no enzymic GST activity with l-chloro-2,4-dinitrobenzene as s ...
BMC Cell Biology
... direct carrier or via an adapter protein binding to the typical NLSs of proteins [7]. NLSs are short regions with a high amount of the basic amino acids arginine, lysine, and proline [16,17]. The main classes of typical NLSs are (i) SV40-like NLSs PKKKRKV, which are composed of a single peptide regi ...
... direct carrier or via an adapter protein binding to the typical NLSs of proteins [7]. NLSs are short regions with a high amount of the basic amino acids arginine, lysine, and proline [16,17]. The main classes of typical NLSs are (i) SV40-like NLSs PKKKRKV, which are composed of a single peptide regi ...
Protein Motif Recognition I Introduction
... 1. How can one try to predict the three-dimensional fold of a protein (either the exact or overall fold)? There are many approaches to this problem. Here we give just a few: • Model all the energetics involved in protein folding, and try to find the structure with lowest free energy. This is a very ...
... 1. How can one try to predict the three-dimensional fold of a protein (either the exact or overall fold)? There are many approaches to this problem. Here we give just a few: • Model all the energetics involved in protein folding, and try to find the structure with lowest free energy. This is a very ...
Biological Molecules: Structure and Methods of Analysis
... Thus, after the nucleic acids have been separated by electrophoresis, the gel is placed under uv light to ...
... Thus, after the nucleic acids have been separated by electrophoresis, the gel is placed under uv light to ...
PowerPoint 簡報
... Sequence alignment: why? • Early in the days of protein and gene sequence analysis, it was discovered that the sequences from related proteins or genes were similar, in the sense that one could align the sequences so that many corresponding residues match. • This discovery was very important: stron ...
... Sequence alignment: why? • Early in the days of protein and gene sequence analysis, it was discovered that the sequences from related proteins or genes were similar, in the sense that one could align the sequences so that many corresponding residues match. • This discovery was very important: stron ...
FYVE-dependent endosomal targeting of an arrestin-related
... important in vivo and mechanistic insights have been unveiled in alternative models such as flies, zebrafish, worms and more recently fungi [5–12]. The social soil amoeba Dictyostelium discoideum is an attractive model system for use in studying the regulation of membrane trafficking events: it is a ...
... important in vivo and mechanistic insights have been unveiled in alternative models such as flies, zebrafish, worms and more recently fungi [5–12]. The social soil amoeba Dictyostelium discoideum is an attractive model system for use in studying the regulation of membrane trafficking events: it is a ...
A homologue of the breast cancer associated gene BARD1 is
... insertions could be identified. The insertion sites were determined in detail by PCR. Figure 4 provides a detailed characterisation of the T-DNA insertions of AtBARD1. The two atbard1 T-DNA insertions are located at the beginning of the gene. Both insertions carry left T-DNA borders at their ends, i ...
... insertions could be identified. The insertion sites were determined in detail by PCR. Figure 4 provides a detailed characterisation of the T-DNA insertions of AtBARD1. The two atbard1 T-DNA insertions are located at the beginning of the gene. Both insertions carry left T-DNA borders at their ends, i ...
Provided for non-commercial research and educational use only
... structure at hand results from the interaction of a small number of components. This is the case for the Min oscillations in E. coli. Other examples exist, and for eukaryotes this concept has also been successfully applied to explain cellular structures.12,13 As soon as the number of components beco ...
... structure at hand results from the interaction of a small number of components. This is the case for the Min oscillations in E. coli. Other examples exist, and for eukaryotes this concept has also been successfully applied to explain cellular structures.12,13 As soon as the number of components beco ...
08A-MembraneStructure
... forming glycolipids, or, more commonly, to proteins, forming glycoproteins. • The oligosaccharides on the external side of the plasma membrane vary from species to species, individual to individual, and even from cell type to cell type within the same individual. • This variation marks each cell typ ...
... forming glycolipids, or, more commonly, to proteins, forming glycoproteins. • The oligosaccharides on the external side of the plasma membrane vary from species to species, individual to individual, and even from cell type to cell type within the same individual. • This variation marks each cell typ ...
Survey of Conserved Alternative Splicing Events
... might have provided plants tolerance against droughts or temperature shifts and given them the ability to live on land. ...
... might have provided plants tolerance against droughts or temperature shifts and given them the ability to live on land. ...
Ribosome Profiling Enables Comprehensive Translation
... Powered by Illumina’s massively parallel, high-throughput sequencing, ribosome profiling allows detailed and accurate in vivo analysis of protein production. The ribosomal profiling technique is adaptable to many organisms, and might be modified to include tissue-specific translational profiling10. ...
... Powered by Illumina’s massively parallel, high-throughput sequencing, ribosome profiling allows detailed and accurate in vivo analysis of protein production. The ribosomal profiling technique is adaptable to many organisms, and might be modified to include tissue-specific translational profiling10. ...
Protein moonlighting
Protein moonlighting (or gene sharing) is a phenomenon by which a protein can perform more than one function. Ancestral moonlighting proteins originally possessed a single function but through evolution, acquired additional functions. Many proteins that moonlight are enzymes; others are receptors, ion channels or chaperones. The most common primary function of moonlighting proteins is enzymatic catalysis, but these enzymes have acquired secondary non-enzymatic roles. Some examples of functions of moonlighting proteins secondary to catalysis include signal transduction, transcriptional regulation, apoptosis, motility, and structural.Protein moonlighting may occur widely in nature. Protein moonlighting through gene sharing differs from the use of a single gene to generate different proteins by alternative RNA splicing, DNA rearrangement, or post-translational processing. It is also different from multifunctionality of the protein, in which the protein has multiple domains, each serving a different function. Protein moonlighting by gene sharing means that a gene may acquire and maintain a second function without gene duplication and without loss of the primary function. Such genes are under two or more entirely different selective constraints.Various techniques have been used to reveal moonlighting functions in proteins. The detection of a protein in unexpected locations within cells, cell types, or tissues may suggest that a protein has a moonlighting function. Furthermore, sequence or structure homology of a protein may be used to infer both primary function as well as secondary moonlighting functions of a protein.The most well-studied examples of gene sharing are crystallins. These proteins, when expressed at low levels in many tissues function as enzymes, but when expressed at high levels in eye tissue, become densely packed and thus form lenses. While the recognition of gene sharing is relatively recent—the term was coined in 1988, after crystallins in chickens and ducks were found to be identical to separately identified enzymes—recent studies have found many examples throughout the living world. Joram Piatigorsky has suggested that many or all proteins exhibit gene sharing to some extent, and that gene sharing is a key aspect of molecular evolution. The genes encoding crystallins must maintain sequences for catalytic function and transparency maintenance function.Inappropriate moonlighting is a contributing factor in some genetic diseases, and moonlighting provides a possible mechanism by which bacteria may become resistant to antibiotics.