MIT 2006: Engineering bacteria to smell good
... Regulating the timing of expression osmY: active in stationary phase & under high osmotic pressure conditions ...
... Regulating the timing of expression osmY: active in stationary phase & under high osmotic pressure conditions ...
Immunodetection of the expression of microsomal proteins encoded
... 6-phosphate) and for the exit of the products, phosphate and glucose [4–7]. The genetic deficiency of the G-6-Pase1 enzyme protein is termed type 1a glycogen storage disease (GSD1a) [2,8]. However, it has been known for a long time that a number of patients with the symptoms of GSD1 are not deficien ...
... 6-phosphate) and for the exit of the products, phosphate and glucose [4–7]. The genetic deficiency of the G-6-Pase1 enzyme protein is termed type 1a glycogen storage disease (GSD1a) [2,8]. However, it has been known for a long time that a number of patients with the symptoms of GSD1 are not deficien ...
Membrane nanodomains in plants: capturing form, function, and
... microscopy, lipid dyes, pharmacological inhibitors of lipid biosynthesis, and lipid biosynthetic mutants have been employed to examine the relationship between the lipid environment and protein activity in plants. They have also been used to identify proteins associated with nanodomains and the path ...
... microscopy, lipid dyes, pharmacological inhibitors of lipid biosynthesis, and lipid biosynthetic mutants have been employed to examine the relationship between the lipid environment and protein activity in plants. They have also been used to identify proteins associated with nanodomains and the path ...
Assembly and function of cell surface structures of the
... symmetries depending on the species (summarized in (Engelhardt & Peters, 1998, Engelhardt, 2007, Konig et al., 2007). One or two (glyco-) protein subunits of molecular masses ranging from 40 to 210 kDa usually make up the S-layers (Konig et al., 2007). The amino acid sequences are highly variable; m ...
... symmetries depending on the species (summarized in (Engelhardt & Peters, 1998, Engelhardt, 2007, Konig et al., 2007). One or two (glyco-) protein subunits of molecular masses ranging from 40 to 210 kDa usually make up the S-layers (Konig et al., 2007). The amino acid sequences are highly variable; m ...
Roles of the mammalian target of rapamycin
... We studied the effects of rapamycin and mTOR-KIs in HeLa cells, using the standard approach of metabolic labelling of newly synthesized proteins with [35 S]methionine. In serumfed cells, treatment with rapamycin for 2 or 6 h had only a small effect on the rate of protein synthesis, inhibiting it by ...
... We studied the effects of rapamycin and mTOR-KIs in HeLa cells, using the standard approach of metabolic labelling of newly synthesized proteins with [35 S]methionine. In serumfed cells, treatment with rapamycin for 2 or 6 h had only a small effect on the rate of protein synthesis, inhibiting it by ...
OsPRP3, a flower specific proline-rich protein of rice, determines
... (Showalter 1993). The composition and structure vary depending on different cell types due to their functional specializations and also can be modified as plants adapt to environmental signaling such as biotic and abiotic stresses (Showalter 1993). The cell walls are composed of about 10% proteins, ...
... (Showalter 1993). The composition and structure vary depending on different cell types due to their functional specializations and also can be modified as plants adapt to environmental signaling such as biotic and abiotic stresses (Showalter 1993). The cell walls are composed of about 10% proteins, ...
Analysis of the distribution of crossing over in the "standard"... confirms earlier meiotic data and indicates a random coincidence with...
... markers like galD-pyrG (average 3%, Table 2) can reliably be arranged in sequence only if an outside marker is reasonably close. For example, suAadE, at a distance of less than 10%, mapped closer to pyrG than galD in all crosses. In contrast, the more distant markers fpaB and uvsF usually but not al ...
... markers like galD-pyrG (average 3%, Table 2) can reliably be arranged in sequence only if an outside marker is reasonably close. For example, suAadE, at a distance of less than 10%, mapped closer to pyrG than galD in all crosses. In contrast, the more distant markers fpaB and uvsF usually but not al ...
The glutamate receptor of the Qp-type activates protein kinase C
... induced translocation reached its maximal value 5 min after phorbol 12,13-dibutyrate addition and remained constant thereafter [19]. A rapid and transient effect similar to the one shown in Fig. 1 has also been observed after activation of other transmembrane receptors as for example the muscarinic ...
... induced translocation reached its maximal value 5 min after phorbol 12,13-dibutyrate addition and remained constant thereafter [19]. A rapid and transient effect similar to the one shown in Fig. 1 has also been observed after activation of other transmembrane receptors as for example the muscarinic ...
Hybrid Functional Petri Nets to Model the Canonical Wnt Pathway
... research as it is one of the main pathways of both cell growth/development as well as genetically linked diseases such as human cancer. • The more understood pathway that involves Wnt proteins is known as the canonical Wnt pathway, and in this pathway, the protein β-catenin plays an important part. ...
... research as it is one of the main pathways of both cell growth/development as well as genetically linked diseases such as human cancer. • The more understood pathway that involves Wnt proteins is known as the canonical Wnt pathway, and in this pathway, the protein β-catenin plays an important part. ...
Peer-reviewed Article PDF
... Hsp90 (heat shock protein 90) is a chaperone protein that assists other proteins to fold properly, stabilizes proteins against heat stress, and aids in protein degradation (Figure 1). It also stabilizes a number of proteins required for tumor growth, which is why Hsp90 inhibitors are investigated as ...
... Hsp90 (heat shock protein 90) is a chaperone protein that assists other proteins to fold properly, stabilizes proteins against heat stress, and aids in protein degradation (Figure 1). It also stabilizes a number of proteins required for tumor growth, which is why Hsp90 inhibitors are investigated as ...
Cardiac O-GlcNAc signaling is increased in hypertrophy and heart
... O-GlcNAc transferase; O-GlcNAcase; aortic stenosis; phosphorylation; glycosylation SUBSEQUENT TO THE DISCOVERY by Torres and Hart (41), OGlcNAc signaling has been implicated in a diverse array of physiological and pathological functions in the cell (17). Although there is a growing recognition that ...
... O-GlcNAc transferase; O-GlcNAcase; aortic stenosis; phosphorylation; glycosylation SUBSEQUENT TO THE DISCOVERY by Torres and Hart (41), OGlcNAc signaling has been implicated in a diverse array of physiological and pathological functions in the cell (17). Although there is a growing recognition that ...
ION BINDING TO BIO
... The stability of protein solutions is governed not only by the macromolecular net charge, salt concentration and valency, but also on the chemical nature of the dissolved ions (1). Traditionally, the latter falls under the category of Hofmeister or ion-specific effects which in recent years has seen ...
... The stability of protein solutions is governed not only by the macromolecular net charge, salt concentration and valency, but also on the chemical nature of the dissolved ions (1). Traditionally, the latter falls under the category of Hofmeister or ion-specific effects which in recent years has seen ...
activator - Cardinal Newman High School
... binding to the operator and blocking RNA polymerase • The repressor is the product of a separate regulatory gene ...
... binding to the operator and blocking RNA polymerase • The repressor is the product of a separate regulatory gene ...
Gene Section MYST4 (MYST histone acetyltransferase (monocytic leukemia) 4)
... both positive (N-terminus) and negative (C-terminus) regulation of transcription, maybe involved in cerebral cortex development, required for RUNX2 -dependent transcriptional activation and ubiquitously expressed in adult human tissues. ...
... both positive (N-terminus) and negative (C-terminus) regulation of transcription, maybe involved in cerebral cortex development, required for RUNX2 -dependent transcriptional activation and ubiquitously expressed in adult human tissues. ...
Novel Function of the Eukaryotic Polypeptide
... AbstractThe mammalian GTP-binding protein GSPT, whose carboxy-terminal sequence is homologous to the eukaryotic elongation factor EF1α, binds to the polypeptide chain releasing factor eRF1 to function as eRF3 in translation termination. However, the amino-terminal domain of GSPT, which contains a p ...
... AbstractThe mammalian GTP-binding protein GSPT, whose carboxy-terminal sequence is homologous to the eukaryotic elongation factor EF1α, binds to the polypeptide chain releasing factor eRF1 to function as eRF3 in translation termination. However, the amino-terminal domain of GSPT, which contains a p ...
Extracting quantitative information from
... • Anders’ starting point this Monday: – Let’s say that we want to study life at the protein level – what technologies do we have at hand? ...
... • Anders’ starting point this Monday: – Let’s say that we want to study life at the protein level – what technologies do we have at hand? ...
Genetic manipulation and quantitative
... 2000) has recently been investigated. However, overexpression of either GOGAT gene has not been reported in plants, including rice. In the first chapter of this review, recent transgenic work expressing sense RNA for NADH-GOGAT in an indica rice is described. Conventional approaches that involve who ...
... 2000) has recently been investigated. However, overexpression of either GOGAT gene has not been reported in plants, including rice. In the first chapter of this review, recent transgenic work expressing sense RNA for NADH-GOGAT in an indica rice is described. Conventional approaches that involve who ...
Julie Thompson – IGBMC
... Similarity-based methods (correlating rates of evolution) (eg. Marcotte, 2000) Comparison of trees, rather than simple co-presence/co-absence (eg. for STRING database, von Mering et al, 2003) ...
... Similarity-based methods (correlating rates of evolution) (eg. Marcotte, 2000) Comparison of trees, rather than simple co-presence/co-absence (eg. for STRING database, von Mering et al, 2003) ...
Most fundamental cellular processes are not
... compare interaction networks between organisms (Yu et al. 2004a). We also defined “regulogs” for transferring regulatory relationships between organisms. We pioneered using 3D molecular structures for analysis of protein networks (Kim et al. 2006; Kim et al. 2008). This work showed that much of the ...
... compare interaction networks between organisms (Yu et al. 2004a). We also defined “regulogs” for transferring regulatory relationships between organisms. We pioneered using 3D molecular structures for analysis of protein networks (Kim et al. 2006; Kim et al. 2008). This work showed that much of the ...
Targeting to the T. gondii plastid
... by the corresponding plastid genome; most plastid proteins are encoded in the nucleus, and imported post-translationally from the cytoplasm into the plastid (Keegstra and Cline, 1999). Proteins destined to reside in plastids that have two membranes, such as the chloroplasts of green plants, typicall ...
... by the corresponding plastid genome; most plastid proteins are encoded in the nucleus, and imported post-translationally from the cytoplasm into the plastid (Keegstra and Cline, 1999). Proteins destined to reside in plastids that have two membranes, such as the chloroplasts of green plants, typicall ...
Advances in affinity purification mass spectrometry of
... type of treatment. Protein interactions that depend on posttranslational modifications (PTMs) can thus be identified and the PTM itself may be mapped by MS. Under ideal circumstances, AP allows the isolation of any given macromolecule in its native state, with its adjacent native macromolecular envi ...
... type of treatment. Protein interactions that depend on posttranslational modifications (PTMs) can thus be identified and the PTM itself may be mapped by MS. Under ideal circumstances, AP allows the isolation of any given macromolecule in its native state, with its adjacent native macromolecular envi ...
+TIPs and Microtubule Regulation. The Beginning of the Plus End in
... (NCBI; http://www.ncbi.nlm.nih.gov/) using sequences from 1TIPs and their binding partners in fungi and metazoans. This analysis indicates that some 1TIPs are conserved in plants (Table I), while others appear to be absent. END BINDING 1 ...
... (NCBI; http://www.ncbi.nlm.nih.gov/) using sequences from 1TIPs and their binding partners in fungi and metazoans. This analysis indicates that some 1TIPs are conserved in plants (Table I), while others appear to be absent. END BINDING 1 ...
PDF (SLMChapter1)
... standard site directed mutagenesis). Because there are no endogenous tRNAs that recognize the TAG codon, only the unnatural amino acid on the amber suppressor tRNA was incorporated at that position of the protein. This was in competition with translation termination, so a mixture of truncated protei ...
... standard site directed mutagenesis). Because there are no endogenous tRNAs that recognize the TAG codon, only the unnatural amino acid on the amber suppressor tRNA was incorporated at that position of the protein. This was in competition with translation termination, so a mixture of truncated protei ...
Cell-Specific Expression of Genes of the Lipid Transfer Protein
... We have characterized three cDNAs from a gene family encoding lipid transfer proteins, LTP, from Arabidopsis thaliana (Wassilewskija). In addition to the already characterized Ltpl, our analysis includes Ltp2 and Ltp3, two sequences previously known as expressed sequence tags (EST) only. The deduced ...
... We have characterized three cDNAs from a gene family encoding lipid transfer proteins, LTP, from Arabidopsis thaliana (Wassilewskija). In addition to the already characterized Ltpl, our analysis includes Ltp2 and Ltp3, two sequences previously known as expressed sequence tags (EST) only. The deduced ...
Antonie van Leeuwenhoek
... Purification of the 40Kprotein and comparison ofits cyanogen bromide fragments with those of other porins. The 40K protein of strain CE 1170 was purified by using the property that it is peptidoglycan-associated (Verhoef et al., 1979). Since the resulting preparation consisted for over ninety percen ...
... Purification of the 40Kprotein and comparison ofits cyanogen bromide fragments with those of other porins. The 40K protein of strain CE 1170 was purified by using the property that it is peptidoglycan-associated (Verhoef et al., 1979). Since the resulting preparation consisted for over ninety percen ...
Protein moonlighting
Protein moonlighting (or gene sharing) is a phenomenon by which a protein can perform more than one function. Ancestral moonlighting proteins originally possessed a single function but through evolution, acquired additional functions. Many proteins that moonlight are enzymes; others are receptors, ion channels or chaperones. The most common primary function of moonlighting proteins is enzymatic catalysis, but these enzymes have acquired secondary non-enzymatic roles. Some examples of functions of moonlighting proteins secondary to catalysis include signal transduction, transcriptional regulation, apoptosis, motility, and structural.Protein moonlighting may occur widely in nature. Protein moonlighting through gene sharing differs from the use of a single gene to generate different proteins by alternative RNA splicing, DNA rearrangement, or post-translational processing. It is also different from multifunctionality of the protein, in which the protein has multiple domains, each serving a different function. Protein moonlighting by gene sharing means that a gene may acquire and maintain a second function without gene duplication and without loss of the primary function. Such genes are under two or more entirely different selective constraints.Various techniques have been used to reveal moonlighting functions in proteins. The detection of a protein in unexpected locations within cells, cell types, or tissues may suggest that a protein has a moonlighting function. Furthermore, sequence or structure homology of a protein may be used to infer both primary function as well as secondary moonlighting functions of a protein.The most well-studied examples of gene sharing are crystallins. These proteins, when expressed at low levels in many tissues function as enzymes, but when expressed at high levels in eye tissue, become densely packed and thus form lenses. While the recognition of gene sharing is relatively recent—the term was coined in 1988, after crystallins in chickens and ducks were found to be identical to separately identified enzymes—recent studies have found many examples throughout the living world. Joram Piatigorsky has suggested that many or all proteins exhibit gene sharing to some extent, and that gene sharing is a key aspect of molecular evolution. The genes encoding crystallins must maintain sequences for catalytic function and transparency maintenance function.Inappropriate moonlighting is a contributing factor in some genetic diseases, and moonlighting provides a possible mechanism by which bacteria may become resistant to antibiotics.