Human Diseases Associated with Form and Function of the Golgi
... where the dystrophin (DMD) protein is not expressed leading to aberrant Golgi organization [35] (Figure 1). However, because this particular condition has been recently reviewed elsewhere [36], we will focus our attention on other Golgi complex proteins, the loss of which induces disease. The first ...
... where the dystrophin (DMD) protein is not expressed leading to aberrant Golgi organization [35] (Figure 1). However, because this particular condition has been recently reviewed elsewhere [36], we will focus our attention on other Golgi complex proteins, the loss of which induces disease. The first ...
GAG binding proteins
... Do Consensus Sequences Exist? Generally, GAG binding proteins contain clustered Lysine and Arginine residues In 1989, Cardin and Weintraub proposed a consensus sequence for heparin binding proteins, B = basic residue ...
... Do Consensus Sequences Exist? Generally, GAG binding proteins contain clustered Lysine and Arginine residues In 1989, Cardin and Weintraub proposed a consensus sequence for heparin binding proteins, B = basic residue ...
Ubiquitin-Proteasome Dependent Regulation of
... lower for plants grown in soil than on MS plates. Because we routinely supply Suc to MS plates, we suspected that Suc may up-regulate the expression of GLK1. Consistent with this idea, it has been shown that carbohydrate metabolism in chloroplasts affects the expression of GLK1 in the nucleus (Papar ...
... lower for plants grown in soil than on MS plates. Because we routinely supply Suc to MS plates, we suspected that Suc may up-regulate the expression of GLK1. Consistent with this idea, it has been shown that carbohydrate metabolism in chloroplasts affects the expression of GLK1 in the nucleus (Papar ...
Disruption of CEP290 microtubule/membrane-binding domains
... function is critical for this process — in CEP290 knockdown experiments,proteins that would normally localize to the cilium fail to do so (14, 19) and cilium formation is disrupted (20, 21). Once the cilium is formed, CEP290 is found at the ciliary transition zone, the region of the organelle just p ...
... function is critical for this process — in CEP290 knockdown experiments,proteins that would normally localize to the cilium fail to do so (14, 19) and cilium formation is disrupted (20, 21). Once the cilium is formed, CEP290 is found at the ciliary transition zone, the region of the organelle just p ...
Archives of microbiology
... digestions, ligations, transformation, preparing electrocompetent cells, and agarose gel electrophoresis were carried out as described by Sambrook and Russell (2001). The purification of the plasmids and products of the polymerase chain reaction (PCR) was carried out using the QIAprep Spin Miniprep ...
... digestions, ligations, transformation, preparing electrocompetent cells, and agarose gel electrophoresis were carried out as described by Sambrook and Russell (2001). The purification of the plasmids and products of the polymerase chain reaction (PCR) was carried out using the QIAprep Spin Miniprep ...
Leukaemia Section del(11)(p12p13) Atlas of Genetics and Cytogenetics in Oncology and Haematology
... Note: Ectopic expression of the LMO2 oncogene due to the removal of a negative regulatory element situated upstream of the LMO2 gene, leading to activation of the proximal LMO2 promoter. In one T-ALL case, this recurrent deletion resulted in a RAG2-LMO2 fusion gene, bringing the LMO2 gene under the ...
... Note: Ectopic expression of the LMO2 oncogene due to the removal of a negative regulatory element situated upstream of the LMO2 gene, leading to activation of the proximal LMO2 promoter. In one T-ALL case, this recurrent deletion resulted in a RAG2-LMO2 fusion gene, bringing the LMO2 gene under the ...
Ros Rickaby, Renee Lee - UK Ocean Acidification Research
... By overexpressing TWCA1 in a pTWIN2 expression vector system (& subsequent purification), we demonstrated that this protein is a catalytically active δCA with both esterase & CO2 hydration activity ...
... By overexpressing TWCA1 in a pTWIN2 expression vector system (& subsequent purification), we demonstrated that this protein is a catalytically active δCA with both esterase & CO2 hydration activity ...
Bacterial Production Lab
... where GB is the rate of “blue” accumulation and f is the fraction of DOM that is labeled “blue”. ...
... where GB is the rate of “blue” accumulation and f is the fraction of DOM that is labeled “blue”. ...
Das ACMG Klassifizierungssystem dient der Einteilung von
... Missense variant in a gene for which primarily truncating variants are known to cause disease Observed in trans with a pathogenic variant for a fully penetrant dominant gene/disorder or observed in cis with a pathogenic variant in any inheritance pattern In-frame deletions/insertions in a repetitive ...
... Missense variant in a gene for which primarily truncating variants are known to cause disease Observed in trans with a pathogenic variant for a fully penetrant dominant gene/disorder or observed in cis with a pathogenic variant in any inheritance pattern In-frame deletions/insertions in a repetitive ...
Nutrient‑regulated gene expression in eukaryotes
... to occur [18]. The dissociation model (Figure 1B) is supported by data indicating that Gal3p is located predominately in the cytoplasm of yeast cells [17] and that the expression of a myristoylated version of the protein (which targets it to the plasma membrane) does not unduly impair the induction ...
... to occur [18]. The dissociation model (Figure 1B) is supported by data indicating that Gal3p is located predominately in the cytoplasm of yeast cells [17] and that the expression of a myristoylated version of the protein (which targets it to the plasma membrane) does not unduly impair the induction ...
A Series of Ubiquitin Binding Factors Connects CDC48/p97 to
... Proteolysis is pivotal for cellular and developmental regulation. Due to its irreversible nature, proteolysis is ideally suited for regulating unidirectional pathways such as cell cycle progression or differentiation. In eukaryotes, selective proteolysis is largely mediated by the ubiquitin/ proteas ...
... Proteolysis is pivotal for cellular and developmental regulation. Due to its irreversible nature, proteolysis is ideally suited for regulating unidirectional pathways such as cell cycle progression or differentiation. In eukaryotes, selective proteolysis is largely mediated by the ubiquitin/ proteas ...
Bioinformatic Software in Web
... In 1975, the introduction of the 2D gel by O’Farrell who began mapping proteins from E. coli. The first major technology to emerge for the identification of proteins was the sequencing of proteins by Edman degradationpicomole MS technology has replaced Edman degradation to identify proteinsfem ...
... In 1975, the introduction of the 2D gel by O’Farrell who began mapping proteins from E. coli. The first major technology to emerge for the identification of proteins was the sequencing of proteins by Edman degradationpicomole MS technology has replaced Edman degradation to identify proteinsfem ...
Genetic data indicate that proteins containing the GGDEF domain
... pYhcK £occulated heavily (Fig. 3B,C). Strong £uorescence was observed in and around these aggregates in the microscope after staining with Calco£uor (data not shown). Plasmid pYhcK caused the strongest aggregation in the liquid culture (Fig. 3C). These results support the data presented by Amikam an ...
... pYhcK £occulated heavily (Fig. 3B,C). Strong £uorescence was observed in and around these aggregates in the microscope after staining with Calco£uor (data not shown). Plasmid pYhcK caused the strongest aggregation in the liquid culture (Fig. 3C). These results support the data presented by Amikam an ...
Tupai eBusiness Systems
... The membrane-associated protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intracellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GC ...
... The membrane-associated protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intracellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GC ...
Ubiquitin-Mediated Control of Plant Hormone
... UPS is also very important for steroid hormone signaling in humans (Lee and Lee, 2012). While some plant hormones undergo long-distance transport and thus have different sites of synthesis and action (e.g. auxin, SL), others appear to be synthesized and function in the same tissue (e.g. BR). As we w ...
... UPS is also very important for steroid hormone signaling in humans (Lee and Lee, 2012). While some plant hormones undergo long-distance transport and thus have different sites of synthesis and action (e.g. auxin, SL), others appear to be synthesized and function in the same tissue (e.g. BR). As we w ...
Proteomic Analysis of the Arabidopsis Nucleolus Suggests Novel
... component proteins and other macromolecules, their interactions, and their responses to changes in cellular activity. The availability of complete genome sequences, together with rapid advances in mass spectrometry methods for analyzing complex polypeptide mixtures, means that it is now possible to ...
... component proteins and other macromolecules, their interactions, and their responses to changes in cellular activity. The availability of complete genome sequences, together with rapid advances in mass spectrometry methods for analyzing complex polypeptide mixtures, means that it is now possible to ...
Using dynamics-based comparisons to predict nucleic acid binding
... Motivation: We have previously demonstrated that proteins may be aligned not only by sequence or structural homology, but also using their dynamical properties. Dynamics-based alignments are sensitive and powerful tools to compare even structurally dissimilar protein families. Here, we propose to us ...
... Motivation: We have previously demonstrated that proteins may be aligned not only by sequence or structural homology, but also using their dynamical properties. Dynamics-based alignments are sensitive and powerful tools to compare even structurally dissimilar protein families. Here, we propose to us ...
DNA Polymorphisms in the β-lactoglobulin and κ–casein Genes
... protein concentration than variant B. It is likely that this difference in amount of β-LG protein is not caused by the amino acid substitutions, but rather by different levels of expression of the corresponding A and B alleles of the β LG gene20. β-Lactoglobulin is amphiphatic and an extremely acid ...
... protein concentration than variant B. It is likely that this difference in amount of β-LG protein is not caused by the amino acid substitutions, but rather by different levels of expression of the corresponding A and B alleles of the β LG gene20. β-Lactoglobulin is amphiphatic and an extremely acid ...
1.4 enzymes 2014
... between substrate and enzyme because there is less kinetic energy, so there are fewer reactions and the rate of reaction is reduced. • As temperatures are increased there is more kinetic energy available, there are more substrate enzyme collisions and the rate of reaction increases ...
... between substrate and enzyme because there is less kinetic energy, so there are fewer reactions and the rate of reaction is reduced. • As temperatures are increased there is more kinetic energy available, there are more substrate enzyme collisions and the rate of reaction increases ...
Biology and computers
... Phylogenetic Structure Find conserved motifs Design of Analysis to deduce function PCR primers Analysis ...
... Phylogenetic Structure Find conserved motifs Design of Analysis to deduce function PCR primers Analysis ...
Lac
... milk. Is the major whey protein in ruminants and pigs. It is not found in milk of many species. The function of ß-LG is unknown; it may be a fatty acid or lipid binding protein. It does have sequence similarities with retinol-binding proteins, but this may not be its function. Generally it is found ...
... milk. Is the major whey protein in ruminants and pigs. It is not found in milk of many species. The function of ß-LG is unknown; it may be a fatty acid or lipid binding protein. It does have sequence similarities with retinol-binding proteins, but this may not be its function. Generally it is found ...
Overexpression of yeast karyopherin Pse1p/Kap121p stimulates the
... Q 1999 Blackwell Science Ltd, Molecular Microbiology, 31, 1499±1511 ...
... Q 1999 Blackwell Science Ltd, Molecular Microbiology, 31, 1499±1511 ...
Multiple Sequence Alignment
... Phylogenetic Structure Find conserved motifs Design of Analysis to deduce function PCR primers Analysis ...
... Phylogenetic Structure Find conserved motifs Design of Analysis to deduce function PCR primers Analysis ...
Document
... • Full text documents (38) randomly selected from EnzyMiner* abstracts • Documents with proteins from 49 UniProt Ids and 24 different species. • Coverage: 488 statements (occurrences of impact information in text), 61 molecular functions and 29 combined mutations. Annotated Information: • Studied pr ...
... • Full text documents (38) randomly selected from EnzyMiner* abstracts • Documents with proteins from 49 UniProt Ids and 24 different species. • Coverage: 488 statements (occurrences of impact information in text), 61 molecular functions and 29 combined mutations. Annotated Information: • Studied pr ...
Hitting the Sweet Spot-Glycans as Targets of Fungal Defense
... positive charge, antiparallel β-sheets and/or α-helices stabilized by multiple disulfide bridges [26,27]. The defensin family includes proteins with antibacterial (see Section 3.1), antifungal and cytotoxic activity from all domains of life. AFP, PAF and BP form a separate class of defensin-like pro ...
... positive charge, antiparallel β-sheets and/or α-helices stabilized by multiple disulfide bridges [26,27]. The defensin family includes proteins with antibacterial (see Section 3.1), antifungal and cytotoxic activity from all domains of life. AFP, PAF and BP form a separate class of defensin-like pro ...
Protein moonlighting
Protein moonlighting (or gene sharing) is a phenomenon by which a protein can perform more than one function. Ancestral moonlighting proteins originally possessed a single function but through evolution, acquired additional functions. Many proteins that moonlight are enzymes; others are receptors, ion channels or chaperones. The most common primary function of moonlighting proteins is enzymatic catalysis, but these enzymes have acquired secondary non-enzymatic roles. Some examples of functions of moonlighting proteins secondary to catalysis include signal transduction, transcriptional regulation, apoptosis, motility, and structural.Protein moonlighting may occur widely in nature. Protein moonlighting through gene sharing differs from the use of a single gene to generate different proteins by alternative RNA splicing, DNA rearrangement, or post-translational processing. It is also different from multifunctionality of the protein, in which the protein has multiple domains, each serving a different function. Protein moonlighting by gene sharing means that a gene may acquire and maintain a second function without gene duplication and without loss of the primary function. Such genes are under two or more entirely different selective constraints.Various techniques have been used to reveal moonlighting functions in proteins. The detection of a protein in unexpected locations within cells, cell types, or tissues may suggest that a protein has a moonlighting function. Furthermore, sequence or structure homology of a protein may be used to infer both primary function as well as secondary moonlighting functions of a protein.The most well-studied examples of gene sharing are crystallins. These proteins, when expressed at low levels in many tissues function as enzymes, but when expressed at high levels in eye tissue, become densely packed and thus form lenses. While the recognition of gene sharing is relatively recent—the term was coined in 1988, after crystallins in chickens and ducks were found to be identical to separately identified enzymes—recent studies have found many examples throughout the living world. Joram Piatigorsky has suggested that many or all proteins exhibit gene sharing to some extent, and that gene sharing is a key aspect of molecular evolution. The genes encoding crystallins must maintain sequences for catalytic function and transparency maintenance function.Inappropriate moonlighting is a contributing factor in some genetic diseases, and moonlighting provides a possible mechanism by which bacteria may become resistant to antibiotics.