Zhang, Zhiyong: An Overview of Protein Structure Prediction: From Homology to Ab Initio
... methods identified more than 90% of homologous pairs, determined from structurestructure similarity comparison, with sequence identity better than 10% and an impressive 38% even for cases with sequence identities between 5% and 9%. The structure models are constructed from the residuals of the struc ...
... methods identified more than 90% of homologous pairs, determined from structurestructure similarity comparison, with sequence identity better than 10% and an impressive 38% even for cases with sequence identities between 5% and 9%. The structure models are constructed from the residuals of the struc ...
Microbiology
... The requirement for the principle carbon source and additional growth factor is very different for different microorganisms, but all naturally produced organic compounds can be used as a source of carbon and energy by some microorganism. ...
... The requirement for the principle carbon source and additional growth factor is very different for different microorganisms, but all naturally produced organic compounds can be used as a source of carbon and energy by some microorganism. ...
HUMAN UTERUS TISSUE LYSATE For Research Use Only
... PBS, pH 7.4, 5.0 M Urea, 2.0 M Thiourea, 50mM DTT, 0.1% SDS ...
... PBS, pH 7.4, 5.0 M Urea, 2.0 M Thiourea, 50mM DTT, 0.1% SDS ...
World Journal of Microbiology and Biotechnology
... thread (although possibly less frequently than wild type cells), and form (some) bacteroid-filled V. hirsuta plant cells (De Maagd et al. 1988, 1989b; Priefer I989). In all these cases the nodules are small, white and devoid of nitrogenase activity. Thus, it appears that complete LPS is not essentia ...
... thread (although possibly less frequently than wild type cells), and form (some) bacteroid-filled V. hirsuta plant cells (De Maagd et al. 1988, 1989b; Priefer I989). In all these cases the nodules are small, white and devoid of nitrogenase activity. Thus, it appears that complete LPS is not essentia ...
TJHHST Biology Olympiad, 2015-16
... 1. Which statement is true about enzymes? Enzymes: A. Are made up of a base containing nitrogen, phosphate, and ribose. B. Have activity that is independent of temperature and pH C. Lose some or all of their normal activity when their 3-D structure is disrupted. D. Provide the activation energy need ...
... 1. Which statement is true about enzymes? Enzymes: A. Are made up of a base containing nitrogen, phosphate, and ribose. B. Have activity that is independent of temperature and pH C. Lose some or all of their normal activity when their 3-D structure is disrupted. D. Provide the activation energy need ...
Database searching
... Multiple alignment of a protein family shows variations in conservation along the length of a protein Example: after aligning many globin proteins, the biologists recognized that the helices region in globins are more conserved than others. ...
... Multiple alignment of a protein family shows variations in conservation along the length of a protein Example: after aligning many globin proteins, the biologists recognized that the helices region in globins are more conserved than others. ...
the elastin gene
... KERATIN - REVISION (cont.) 5. How long are individual keratin molecules? How are they incorporated as subunits into keratin superstructures? What is the significance of "acidic" and "basic" keratins in this respect? 6. What are the main crosslinks in keratin? How can they be broken to partially sol ...
... KERATIN - REVISION (cont.) 5. How long are individual keratin molecules? How are they incorporated as subunits into keratin superstructures? What is the significance of "acidic" and "basic" keratins in this respect? 6. What are the main crosslinks in keratin? How can they be broken to partially sol ...
Membrane Protein Sorting in the Yeast Secretory Pathway
... secretory pathway of eukaryotic cells have targeting information that directs retention in or sorting to the appropriate compartment (Pfeffer and Rothman, 1987). In the absence of retention or sorting signals, soluble proteins of the secretory pathway are secreted; thus, the default pathway for thes ...
... secretory pathway of eukaryotic cells have targeting information that directs retention in or sorting to the appropriate compartment (Pfeffer and Rothman, 1987). In the absence of retention or sorting signals, soluble proteins of the secretory pathway are secreted; thus, the default pathway for thes ...
Stabilization by GroEL, a Molecular Chaperone, and a Periplasmic
... partment can be compared to the lumen of the endoplasmic reticulum (ER) in eukaryotic cells. Many proteins are found in the periplasm, but the physiological functions of only a few such proteins are known. Furthermore, the periplasm of Escherichia coli has been of great interest with respect to the ...
... partment can be compared to the lumen of the endoplasmic reticulum (ER) in eukaryotic cells. Many proteins are found in the periplasm, but the physiological functions of only a few such proteins are known. Furthermore, the periplasm of Escherichia coli has been of great interest with respect to the ...
GEFs: master regulators of G
... understand the complex and highlycoupled network of signaling events in which GEFs participate. The elaborate suites of regulatory modules with which many GEFs are adorned suggest that G-protein activation is localized, contingent and temporally defined. ...
... understand the complex and highlycoupled network of signaling events in which GEFs participate. The elaborate suites of regulatory modules with which many GEFs are adorned suggest that G-protein activation is localized, contingent and temporally defined. ...
Document
... Each type of base on one strand forms a bond with just one type of base on the other strand Here, purines form hydrogen bonds to pyrimidines, with A bonding only to T, and C bonding only to G DNA sequence ...
... Each type of base on one strand forms a bond with just one type of base on the other strand Here, purines form hydrogen bonds to pyrimidines, with A bonding only to T, and C bonding only to G DNA sequence ...
Plant nuclear proteomics inside the cell maestro
... microscopy techniques, about 30 different compartments [14] have recently been discovered. The uniqueness of the nucleus is shown by the fact that all of its subcompartments are membrane-less, self-organizing entities that pass through a state of disassembly ⁄ reassembly during cell division. In fac ...
... microscopy techniques, about 30 different compartments [14] have recently been discovered. The uniqueness of the nucleus is shown by the fact that all of its subcompartments are membrane-less, self-organizing entities that pass through a state of disassembly ⁄ reassembly during cell division. In fac ...
Pierce Antibodies - Fisher Scientific
... Over 30,000 antibodies in 42 research areas. Thermo Scientific Pierce Primary Antibodies are developed for a wide variety of application needs. All of our antibodies are validated and guaranteed to perform in the stated application and species. Also, our Innovator’s Program rewards researchers for s ...
... Over 30,000 antibodies in 42 research areas. Thermo Scientific Pierce Primary Antibodies are developed for a wide variety of application needs. All of our antibodies are validated and guaranteed to perform in the stated application and species. Also, our Innovator’s Program rewards researchers for s ...
pGEX-5X-3 GST Expression Vector
... and bulk media products available to purify GST Fusion proteins. For manual purification of sample volumes up to 600 µl use GST SpinTrap™ microspin columns or GST MultiTrap™ 4B 96-well plates. For sample volumes between 600 µl and 10 ml use GST GraviTrap™ gravity flow column. Where sample volumes ar ...
... and bulk media products available to purify GST Fusion proteins. For manual purification of sample volumes up to 600 µl use GST SpinTrap™ microspin columns or GST MultiTrap™ 4B 96-well plates. For sample volumes between 600 µl and 10 ml use GST GraviTrap™ gravity flow column. Where sample volumes ar ...
DV axis HT12
... Flies and frogs • Dorsal neural tube of the vertebrate and ventral nerve cord of the fly appear to be generated by the same set of instructions. fly ...
... Flies and frogs • Dorsal neural tube of the vertebrate and ventral nerve cord of the fly appear to be generated by the same set of instructions. fly ...
Setting sail for glucose homeostasis with the AKAP150PP2Banchor
... glucose homeostasis in mice (Figure 1). However, it is likely that there are AKAP150-independent mechanisms regulating insulin secretion since islets from AKAP150KO mice continued to respond to glucose stimulation and secrete insulin in both static and dynamic conditions, albeit at lower levels comp ...
... glucose homeostasis in mice (Figure 1). However, it is likely that there are AKAP150-independent mechanisms regulating insulin secretion since islets from AKAP150KO mice continued to respond to glucose stimulation and secrete insulin in both static and dynamic conditions, albeit at lower levels comp ...
cDNA cloning, expression and chromosomal localization of the
... located on a protrusion of the protein (Eklund et al., 1991). So far, only two thioredoxins have been described in mammalian cells: Trx1 is a cytosolic protein with many different functions most of them depending on the redox active site (Holmgren, 1985) and Trx2 is a mitocondrial protein whose only ...
... located on a protrusion of the protein (Eklund et al., 1991). So far, only two thioredoxins have been described in mammalian cells: Trx1 is a cytosolic protein with many different functions most of them depending on the redox active site (Holmgren, 1985) and Trx2 is a mitocondrial protein whose only ...
Evolution Expression Level, and Interactivity Are Correlated in
... the compared species. The highest PGL value obtained here, 0.49, is the maximum only for the genes and species considered in this analysis; as additional genomes are included, greater PGL values will result. ...
... the compared species. The highest PGL value obtained here, 0.49, is the maximum only for the genes and species considered in this analysis; as additional genomes are included, greater PGL values will result. ...
Chapter 5: Extensions of Mendelian Inheritance
... This will be useful later in additional discussions of gene interactions. Another example of a gene interaction is complementation (Refer to Figure 5.14). This refers to the production of offspring with a wild-type phenotype from parents that both display the same or recessive phenotypes. This secti ...
... This will be useful later in additional discussions of gene interactions. Another example of a gene interaction is complementation (Refer to Figure 5.14). This refers to the production of offspring with a wild-type phenotype from parents that both display the same or recessive phenotypes. This secti ...
Document
... outcome of a sequencing project are masses of raw data The challenge is to turn these raw data into biological knowledge A valuable tool for this challenge is an automated diagnostic pipe through which newly determined sequences can be streamlined ...
... outcome of a sequencing project are masses of raw data The challenge is to turn these raw data into biological knowledge A valuable tool for this challenge is an automated diagnostic pipe through which newly determined sequences can be streamlined ...
Zika Virus Proteins - Peptides International
... been mounting evidence that Zika fever in Aedes mosquitoes, such as A. aepregnant women can cause abnormal brain gypti and A. albopictus. The Zika virus is redevelopment in their fetuses by mother-tolated to the dengue, yellow fever, Japanese child transmission, which may result in misencephalitis, ...
... been mounting evidence that Zika fever in Aedes mosquitoes, such as A. aepregnant women can cause abnormal brain gypti and A. albopictus. The Zika virus is redevelopment in their fetuses by mother-tolated to the dengue, yellow fever, Japanese child transmission, which may result in misencephalitis, ...
Molecular Chaperones - Cellular Machines for Protein Folding
... respectively, which are both required for the viability of E. coli.[25] Thus, at least one essential E. coli protein cannot fold without assistance from the GroE chaperone. 2.1.1. Structure of the GroE Chaperone The most striking feature of GroEL is its quaternary structure, which resembles a barrel ...
... respectively, which are both required for the viability of E. coli.[25] Thus, at least one essential E. coli protein cannot fold without assistance from the GroE chaperone. 2.1.1. Structure of the GroE Chaperone The most striking feature of GroEL is its quaternary structure, which resembles a barrel ...
CHAPTER FOUR Pseudomonas aeruginosa INFLUENCED PLANKTONIC AND BIOFILM POPULATIONS BASED UPON
... sessile multicellular communities known as biofilms (Costerton et al., 1987; 1995). The transition to surface-attached (biofilm) growth is known to result in diverse changes in gene expression, which causes the attaching cells to become phenotypically and metabolically distinct from their planktonic ...
... sessile multicellular communities known as biofilms (Costerton et al., 1987; 1995). The transition to surface-attached (biofilm) growth is known to result in diverse changes in gene expression, which causes the attaching cells to become phenotypically and metabolically distinct from their planktonic ...
AGRA: analysis of gene ranking algorithms
... AGRA then queries FACTA with these Uniprot identifiers and maximum 50 most important biomedical concepts (ranked by their frequencies of appearing in the MEDLINE abstracts) from each category are extracted. Concepts that are gathered in this step represent six BCS categories of each associated prote ...
... AGRA then queries FACTA with these Uniprot identifiers and maximum 50 most important biomedical concepts (ranked by their frequencies of appearing in the MEDLINE abstracts) from each category are extracted. Concepts that are gathered in this step represent six BCS categories of each associated prote ...
Protein moonlighting
Protein moonlighting (or gene sharing) is a phenomenon by which a protein can perform more than one function. Ancestral moonlighting proteins originally possessed a single function but through evolution, acquired additional functions. Many proteins that moonlight are enzymes; others are receptors, ion channels or chaperones. The most common primary function of moonlighting proteins is enzymatic catalysis, but these enzymes have acquired secondary non-enzymatic roles. Some examples of functions of moonlighting proteins secondary to catalysis include signal transduction, transcriptional regulation, apoptosis, motility, and structural.Protein moonlighting may occur widely in nature. Protein moonlighting through gene sharing differs from the use of a single gene to generate different proteins by alternative RNA splicing, DNA rearrangement, or post-translational processing. It is also different from multifunctionality of the protein, in which the protein has multiple domains, each serving a different function. Protein moonlighting by gene sharing means that a gene may acquire and maintain a second function without gene duplication and without loss of the primary function. Such genes are under two or more entirely different selective constraints.Various techniques have been used to reveal moonlighting functions in proteins. The detection of a protein in unexpected locations within cells, cell types, or tissues may suggest that a protein has a moonlighting function. Furthermore, sequence or structure homology of a protein may be used to infer both primary function as well as secondary moonlighting functions of a protein.The most well-studied examples of gene sharing are crystallins. These proteins, when expressed at low levels in many tissues function as enzymes, but when expressed at high levels in eye tissue, become densely packed and thus form lenses. While the recognition of gene sharing is relatively recent—the term was coined in 1988, after crystallins in chickens and ducks were found to be identical to separately identified enzymes—recent studies have found many examples throughout the living world. Joram Piatigorsky has suggested that many or all proteins exhibit gene sharing to some extent, and that gene sharing is a key aspect of molecular evolution. The genes encoding crystallins must maintain sequences for catalytic function and transparency maintenance function.Inappropriate moonlighting is a contributing factor in some genetic diseases, and moonlighting provides a possible mechanism by which bacteria may become resistant to antibiotics.