Body Protein Synthesis
... liver cell quickly rises to a higher level again. We thus have three phases during which liver RNA metabolism can be explored: (a)the fully depleted state after a few days on the protein-free diet; (b) theinitial period of rapid loss of RNA from the liver; (c) the response to refeeding with protein. ...
... liver cell quickly rises to a higher level again. We thus have three phases during which liver RNA metabolism can be explored: (a)the fully depleted state after a few days on the protein-free diet; (b) theinitial period of rapid loss of RNA from the liver; (c) the response to refeeding with protein. ...
File - Nepal Pharmacy
... 4. Selectively permeable - allows some molecules in, others are kept out ...
... 4. Selectively permeable - allows some molecules in, others are kept out ...
Retinoblastoma Protein Contains a C-terminal - Bio
... requirement for a (serine/threonine)-proline (S/T-P) phosphoacceptor site and a preference for a basic residue at position 13 (where the S/T position is position 0) (36, 70, 83, 85, 98). It is clear, however, that the cyclin moiety contributes to substrate specificity. For example, cyclin A-cdc2 but ...
... requirement for a (serine/threonine)-proline (S/T-P) phosphoacceptor site and a preference for a basic residue at position 13 (where the S/T position is position 0) (36, 70, 83, 85, 98). It is clear, however, that the cyclin moiety contributes to substrate specificity. For example, cyclin A-cdc2 but ...
PLoS Pathogens
... remain elusive. HopZ1 is a P. syringae T3SE that belongs to the widely distributed YopJ family of cysteine proteases/acetyltransferases produced by both plant and animal bacterial pathogens [9]. The YopJ-like T3SEs share a conserved catalytic core, consisting of three key amino acid residues (histid ...
... remain elusive. HopZ1 is a P. syringae T3SE that belongs to the widely distributed YopJ family of cysteine proteases/acetyltransferases produced by both plant and animal bacterial pathogens [9]. The YopJ-like T3SEs share a conserved catalytic core, consisting of three key amino acid residues (histid ...
Linköping University Post Print Histone Variants and Their Post-Translational
... basic proteins with acid, a protocol that has extensively been used with other cell types [21–25] (outlined in Figure1A, left). SDSPAGE separation of the proteins extracted by acid from isolated nuclei revealed a distinct protein pattern (Figure 1B, left lane), and analysis of the major proteins by ...
... basic proteins with acid, a protocol that has extensively been used with other cell types [21–25] (outlined in Figure1A, left). SDSPAGE separation of the proteins extracted by acid from isolated nuclei revealed a distinct protein pattern (Figure 1B, left lane), and analysis of the major proteins by ...
The role of mTOR signaling in the regulation of protein synthesis
... stimuli is largely mediated by a protein kinase called the mammalian (or the mechanistic) target of rapamycin (mTOR), which exists in at least two characteristically distinct complexes: (a) the rapamycinsensitive mTOR complex 1 (mTORC1), and (b) the rapamycininsensitive mTOR complex 2 (mTORC2) (Ma a ...
... stimuli is largely mediated by a protein kinase called the mammalian (or the mechanistic) target of rapamycin (mTOR), which exists in at least two characteristically distinct complexes: (a) the rapamycinsensitive mTOR complex 1 (mTORC1), and (b) the rapamycininsensitive mTOR complex 2 (mTORC2) (Ma a ...
Regulation of E. coli Glycogen Phosphorylase Activity by HPr
... between HPr and GP and the metabolic crosstalk between glucose uptake and glycogen breakdown is discussed. Activation of Glycogen Phosphorylase by Interaction with HPr While there was no history suggesting a role for HPr in metabolic regulation in enteric bacteria, there was a theoretical rationale ...
... between HPr and GP and the metabolic crosstalk between glucose uptake and glycogen breakdown is discussed. Activation of Glycogen Phosphorylase by Interaction with HPr While there was no history suggesting a role for HPr in metabolic regulation in enteric bacteria, there was a theoretical rationale ...
Inhibition of Protein Synthesis by Vaccinia Virus. II. Studies on the
... second hypothesis states that the inhibition of protein synthesis can be correlated with virus-induced RNA synthesis (Babtanian, I975; Bablanian et al. I978). It was found that while vaccinia-infected (300 particles/cell), cycloheximide-treated (300/zg/ml), L and HeLa cells failed to resume protein ...
... second hypothesis states that the inhibition of protein synthesis can be correlated with virus-induced RNA synthesis (Babtanian, I975; Bablanian et al. I978). It was found that while vaccinia-infected (300 particles/cell), cycloheximide-treated (300/zg/ml), L and HeLa cells failed to resume protein ...
Polymerization and nucleic acid-binding
... remain active in most modern mammalian species, including humans (4,5). They were sufficiently deleterious in early hominids to have been subject to negative selection (6), and may well still be in modern humans for they are a significant source of genetic diversity, defects and rearrangements (5,7–10 ...
... remain active in most modern mammalian species, including humans (4,5). They were sufficiently deleterious in early hominids to have been subject to negative selection (6), and may well still be in modern humans for they are a significant source of genetic diversity, defects and rearrangements (5,7–10 ...
Expansion of the phragmoplast during plant cytokinesis: a MAPK
... Arabidopsis thaliana phragmoplast-associated kinesinrelated protein1 (AtPAKRP1) is localized to the center of the phragmoplast, and microinjection of anti-AtPAKRP1 antibodies or truncated AtPAKRP1 proteins into tobacco BY-2 cells leads to the disorganization of phragmoplast MTs [19••]. Carrot DcKRP1 ...
... Arabidopsis thaliana phragmoplast-associated kinesinrelated protein1 (AtPAKRP1) is localized to the center of the phragmoplast, and microinjection of anti-AtPAKRP1 antibodies or truncated AtPAKRP1 proteins into tobacco BY-2 cells leads to the disorganization of phragmoplast MTs [19••]. Carrot DcKRP1 ...
pdf: Xu et al. 2008
... of Biology, University of North Carolina, Chapel Hill, North Carolina 27599-3280 of Biology, University of Massachusetts, Amherst, Massachusetts 01003 ...
... of Biology, University of North Carolina, Chapel Hill, North Carolina 27599-3280 of Biology, University of Massachusetts, Amherst, Massachusetts 01003 ...
Roles for Regulator of G Protein Signaling Proteins in Synaptic
... expression of RGS2 (Perez et al., 2001). The numbers of apical and basilar spines of dendrites in CA1 hippocampal neurons are also significantly decreased in RGS2-KO mice (OliveiraDos-Santos et al., 2000), with the amount of spines being indicative of overall synapse numbers and synaptic plasticity ...
... expression of RGS2 (Perez et al., 2001). The numbers of apical and basilar spines of dendrites in CA1 hippocampal neurons are also significantly decreased in RGS2-KO mice (OliveiraDos-Santos et al., 2000), with the amount of spines being indicative of overall synapse numbers and synaptic plasticity ...
LIPID-TRANSFER PROTEINS IN PLANTS
... When the fluorescent phospholipids leave the donor membranes to reach the acceptor liposomes, the fluorescence increases because of a decrease in the quenching in the donor membrane. This assay allows a continuous monitoring of the lipid transfer (22, 73). These assays have been used to determine th ...
... When the fluorescent phospholipids leave the donor membranes to reach the acceptor liposomes, the fluorescence increases because of a decrease in the quenching in the donor membrane. This assay allows a continuous monitoring of the lipid transfer (22, 73). These assays have been used to determine th ...
Contribution of molecular chaperones to protein folding in the
... vitro studies with purified globular proteins under well-defined conditions. In their seminal studies, Anfinsen and colleagues observed that ribonuclease A, which had been unfolded by chemical denaturation, could refold spontaneously into its correct functional conformation upon dilution of the dena ...
... vitro studies with purified globular proteins under well-defined conditions. In their seminal studies, Anfinsen and colleagues observed that ribonuclease A, which had been unfolded by chemical denaturation, could refold spontaneously into its correct functional conformation upon dilution of the dena ...
The proPO and clotting system in crustaceans
... ester in the vertebrate complement molecule becomes highly active and it can react with the hydroxyl or amino group on biological surfaces, which leads to immobilization of this molecule to a foreign surface. Whether this mechanism occurs with invertebrate proPOs has to be further clarified. By comp ...
... ester in the vertebrate complement molecule becomes highly active and it can react with the hydroxyl or amino group on biological surfaces, which leads to immobilization of this molecule to a foreign surface. Whether this mechanism occurs with invertebrate proPOs has to be further clarified. By comp ...
DELIVERY OF PROTEIN USING NANOPARTICLE Research Article A.ROBIN
... of Trypsin which was bounded to Au nanoparticles is active even after instability caused by aggregation of proteins on Nanoparticles. This also shows that any drug or enzyme or protein can be bound to Au nanoparticles and used as drug carrier to the target site. Alkaline phosphatase assay Alkaline p ...
... of Trypsin which was bounded to Au nanoparticles is active even after instability caused by aggregation of proteins on Nanoparticles. This also shows that any drug or enzyme or protein can be bound to Au nanoparticles and used as drug carrier to the target site. Alkaline phosphatase assay Alkaline p ...
Document
... First axis: separates Integral Inner Membrane Proteins (IIMP) from the rest; driven by opposition between charged and large hydrophobic residues Second axis: separates proteins according to an opposition driven by the G+C content of the first codon base Third axis: separates proteins by their conten ...
... First axis: separates Integral Inner Membrane Proteins (IIMP) from the rest; driven by opposition between charged and large hydrophobic residues Second axis: separates proteins according to an opposition driven by the G+C content of the first codon base Third axis: separates proteins by their conten ...
Sequential steps in clathrin-mediated synaptic vesicle endocytosis
... clathrin assembly and which appears to be critical for the generation of synaptic vesicles with an homogenous size. In Drosophila and C. elegans lacking AP180-like proteins, nerve terminals still contain synaptic vesicles, but their average size is larger and the size variability is increased when c ...
... clathrin assembly and which appears to be critical for the generation of synaptic vesicles with an homogenous size. In Drosophila and C. elegans lacking AP180-like proteins, nerve terminals still contain synaptic vesicles, but their average size is larger and the size variability is increased when c ...
The syndapin protein family: linking membrane trafficking with the
... Syndapins – also called PACSINs – are highly conserved Src-homology 3 (SH3)-domain-containing proteins that seem to exist in all multicellular eukaryotes. They interact with the large GTPase dynamin and several other proteins implicated in vesicle trafficking. Syndapin-dynamin complexes appear to pl ...
... Syndapins – also called PACSINs – are highly conserved Src-homology 3 (SH3)-domain-containing proteins that seem to exist in all multicellular eukaryotes. They interact with the large GTPase dynamin and several other proteins implicated in vesicle trafficking. Syndapin-dynamin complexes appear to pl ...
Microbiology
... 6 min at 80 W. The unbroken cells were removed by centrifugation for 10 min at 103g. The supernatant was centrifuged at 6 x 104g for 30 min at 5 "C. The pellet, constituting the crude envelope fraction, was resuspended in 8 ml of a 10 mM Tris/HCl buffer (pH 8.0) containing 0.5 Yo N-laurylsarcosine. ...
... 6 min at 80 W. The unbroken cells were removed by centrifugation for 10 min at 103g. The supernatant was centrifuged at 6 x 104g for 30 min at 5 "C. The pellet, constituting the crude envelope fraction, was resuspended in 8 ml of a 10 mM Tris/HCl buffer (pH 8.0) containing 0.5 Yo N-laurylsarcosine. ...
A biofilm-forming marine bacterium producing proteins
... another cell bound fraction, non soluble in a salt solution (36g/l). None of them is pure. EPS1 consists mainly in a majority of carbohydrates while the two others consist mainly in a majority of proteins. Gel electrophoresis analysis showed different EPS proteins composition with molecular weight ...
... another cell bound fraction, non soluble in a salt solution (36g/l). None of them is pure. EPS1 consists mainly in a majority of carbohydrates while the two others consist mainly in a majority of proteins. Gel electrophoresis analysis showed different EPS proteins composition with molecular weight ...
Neuronal polarity: from extracellular signals to
... Ubiquitin–proteasome system Ubiquitin is a small regulatory protein. Ubiquitylation refers to the post-translational modification of a protein by the covalent attachment of one or more ubiquitin monomers. After a chain of at least four ubiquitin peptides are attached to a lysine on a substrate prote ...
... Ubiquitin–proteasome system Ubiquitin is a small regulatory protein. Ubiquitylation refers to the post-translational modification of a protein by the covalent attachment of one or more ubiquitin monomers. After a chain of at least four ubiquitin peptides are attached to a lysine on a substrate prote ...
Diversity of the Superfamily of Phloem Lectins (Phloem Protein 2) in
... Apiales, which include Apiacae, are unrelated to Cucurbitales and belong to highly divergent subgroups, asterids and rosids, respectively. However, alignment of cucurbit and celery sequences showed a succession of four conserved motifs (A, B, C, and D) common to sequences in the two subgroups (Fig. ...
... Apiales, which include Apiacae, are unrelated to Cucurbitales and belong to highly divergent subgroups, asterids and rosids, respectively. However, alignment of cucurbit and celery sequences showed a succession of four conserved motifs (A, B, C, and D) common to sequences in the two subgroups (Fig. ...
Effects of phosphatidylethanolamine glycation on lipid–protein
... glycation by forming adducts with PE. Moreover, these authors found these complexes in human red blood cells, suggesting that this compound may act as a lipid glycation inhibitor in vivo, and demonstrated that supplementation of the diet of diabetic rats with pyridoxal 5 -phosphate reduces the leve ...
... glycation by forming adducts with PE. Moreover, these authors found these complexes in human red blood cells, suggesting that this compound may act as a lipid glycation inhibitor in vivo, and demonstrated that supplementation of the diet of diabetic rats with pyridoxal 5 -phosphate reduces the leve ...
Protein phosphorylation
Protein phosphorylation is a post-translational modification of proteins in which an amino acid residue is phosphorylated by a protein kinase by the addition of a covalently bound phosphate group. Phosphorylation alters the structural conformation of a protein, causing it to become activated, deactivated, or modifying its function. The reverse reaction of phosphorylation is called dephosphorylation, and is catalyzed by protein phosphatases. Protein kinases and phosphatases work independently and in a balance to regulate the function of proteins. The amino acids most commonly phosphorylated are serine, threonine, and tyrosine in eukaryotes, and histidine in prokaryotes, which play important and well-characterized roles in signaling pathways and metabolism. However, many other amino acids can also be phosphorylated, including arginine, lysine, and cysteine. Protein phosphorylation was first reported in 1906 by Phoebus Levene at the Rockefeller Institute for Medical Research with the discovery of phosphorylated vitellin. However, it was nearly 50 years until the enzymatic phosphorylation of proteins by protein kinases was discovered.