The RNA-binding protein repertoire of embryonic
... investigated whether a comparable amount of RNA could be pulled down by quantitative reverse transcription PCR (RT-PCR) (Fig. 1d). Endogenous Gapdh mRNA and exogenous poly(A)-tailed luciferase mRNA (spike-in) that was mixed with lysate after cell lysis were pulled down from both the irradiated and n ...
... investigated whether a comparable amount of RNA could be pulled down by quantitative reverse transcription PCR (RT-PCR) (Fig. 1d). Endogenous Gapdh mRNA and exogenous poly(A)-tailed luciferase mRNA (spike-in) that was mixed with lysate after cell lysis were pulled down from both the irradiated and n ...
PROTEIN METABOLISM
... the general nature of the genetic code came from many types of experiments, including genetic experiments on the effects of deletion and insertion mutations. Inserting or deleting one base pair (shown here in the mRNA transcript) alters the sequence of triplets in a nonoverlapping code; all amino ac ...
... the general nature of the genetic code came from many types of experiments, including genetic experiments on the effects of deletion and insertion mutations. Inserting or deleting one base pair (shown here in the mRNA transcript) alters the sequence of triplets in a nonoverlapping code; all amino ac ...
Characterization of a novel phosphatidylinositol 3
... as novel zinc fingers that are present in a variety of proteins implicated in vesicular trafficking. These 70 residue domains are highly conserved between species and are named after the first four proteins (Fab1p, YOTB, Vac1p and EEA1) shown to contain them [1]. Although first identified in protein ...
... as novel zinc fingers that are present in a variety of proteins implicated in vesicular trafficking. These 70 residue domains are highly conserved between species and are named after the first four proteins (Fab1p, YOTB, Vac1p and EEA1) shown to contain them [1]. Although first identified in protein ...
109 y+-TYPE CATIONIC AMINO ACID TRANSPORT
... cDNA clones from two closely related lymphoma cell lines (MacLeod et al. 1984, 1985) and resulted in the isolation of several novel cDNAs. One of the novel genes was named Tea, because it is induced early in the response of normal T cells to mitogens (MacLeod et al. 1990a). The cDNA sequence reveale ...
... cDNA clones from two closely related lymphoma cell lines (MacLeod et al. 1984, 1985) and resulted in the isolation of several novel cDNAs. One of the novel genes was named Tea, because it is induced early in the response of normal T cells to mitogens (MacLeod et al. 1990a). The cDNA sequence reveale ...
Cell Cycle in the Fucus Zygote Parallels a Somatic Cell
... these checkpoints, and their regulation after checkpoint activation is usually correlated with changes in their phosphorylation status as well as with associations with regulatory molecules such as CDK inhibitors (Lew and Kornbluth, 1996; Rudner and Murray, 1996; Hardwick, 1998). These checkpoints a ...
... these checkpoints, and their regulation after checkpoint activation is usually correlated with changes in their phosphorylation status as well as with associations with regulatory molecules such as CDK inhibitors (Lew and Kornbluth, 1996; Rudner and Murray, 1996; Hardwick, 1998). These checkpoints a ...
BTK, THE TYROSINE KINASE AFFECTED IN X-LINKED
... Btk is involved in radiation-induced apoptosis in DT-40 lymphoma B cells. Btk, but not Lyn, Syk or Csk, mediates the radiation-induced apoptosis in a kinase domain-dependent manner (53). Recently, Xid B cells stimulated through surface IgM but not CD40 were shown to undergo apoptotic cell death (46) ...
... Btk is involved in radiation-induced apoptosis in DT-40 lymphoma B cells. Btk, but not Lyn, Syk or Csk, mediates the radiation-induced apoptosis in a kinase domain-dependent manner (53). Recently, Xid B cells stimulated through surface IgM but not CD40 were shown to undergo apoptotic cell death (46) ...
RalA ACTIVATION ASSAY BIOCHEM KIT
... B. Growth and Treatment of Cell Lines The health and responsiveness of your cell line is the single most important parameter for the success and reproducibility of RalA activation assays. Time should be taken to read this section and to carefully maintain cell lines in accordance with the guidelines ...
... B. Growth and Treatment of Cell Lines The health and responsiveness of your cell line is the single most important parameter for the success and reproducibility of RalA activation assays. Time should be taken to read this section and to carefully maintain cell lines in accordance with the guidelines ...
Oligosaccharide signalling for defense responses in plant
... rice cells [87, 88], tomato cells [4] and soybean root and cultured cells [22] by using radio-labelled, elicitor-active derivatives of the oligosaccharide. The binding speci®city of these sites corresponded well with the elicitor activity of chitin oligosaccharides in these systems, re¯ecting the di ...
... rice cells [87, 88], tomato cells [4] and soybean root and cultured cells [22] by using radio-labelled, elicitor-active derivatives of the oligosaccharide. The binding speci®city of these sites corresponded well with the elicitor activity of chitin oligosaccharides in these systems, re¯ecting the di ...
Ion homeostasis, channels, and transporters: an update on cellular
... controlling the accessibility of the transported ion to a pore domain. In turn, the pore domain acts as the pathway or conduit for ions moving from one side of the membrane to the other. Because the actual transport of ions through channels does not require a defined sequence of energetic interactio ...
... controlling the accessibility of the transported ion to a pore domain. In turn, the pore domain acts as the pathway or conduit for ions moving from one side of the membrane to the other. Because the actual transport of ions through channels does not require a defined sequence of energetic interactio ...
Molecular Components of the Bacterial Cytoskeleton
... with the endosymbiont model for the origins of chloroplasts and mitochondria, FtsZ is also found in these organelles in some eukaryotes (Osteryoung and Vierling 1995; Beech et al. 2000), where it retains a conserved function in organelle division (Osteryoung and McAndrew 2001; Vitha et al. 2001). In ...
... with the endosymbiont model for the origins of chloroplasts and mitochondria, FtsZ is also found in these organelles in some eukaryotes (Osteryoung and Vierling 1995; Beech et al. 2000), where it retains a conserved function in organelle division (Osteryoung and McAndrew 2001; Vitha et al. 2001). In ...
Post-transcriptional regulation of gene expression in innate immunity
... upregulated by LPS, as well as by IFNγ and interleukin‑6 (IL‑6)20. Both MD2B and MD2s proteins bind TLR4 as efficiently as full-length MD2 but they fail to mediate signalling. MD2B inhibits cell surface expression of mouse TLR4 (REF. 19), and MD2s inhibits the binding of full-length MD2 to TLR4 (REF ...
... upregulated by LPS, as well as by IFNγ and interleukin‑6 (IL‑6)20. Both MD2B and MD2s proteins bind TLR4 as efficiently as full-length MD2 but they fail to mediate signalling. MD2B inhibits cell surface expression of mouse TLR4 (REF. 19), and MD2s inhibits the binding of full-length MD2 to TLR4 (REF ...
Gene Section KSR1 (kinase suppressor of ras 1)
... Raf/MEK/ERK kinase cascade. However, there are several published studies that sustain that KSR1 has catalytic activity. KSR1 is recognized as a pseudokinase, since mammalian KSR1 does not possess the lysine responsible for ATP orientation and hydrolysis in the putative kinase domain. This lysine is ...
... Raf/MEK/ERK kinase cascade. However, there are several published studies that sustain that KSR1 has catalytic activity. KSR1 is recognized as a pseudokinase, since mammalian KSR1 does not possess the lysine responsible for ATP orientation and hydrolysis in the putative kinase domain. This lysine is ...
Antioxidative Activities of Hydrolysates from Duck Egg White Using
... metabolic processes in food system or human body (Wang et al., 2006). The oxidative stress caused by free radicals and ROS would damage lipid and protein that resulted in cell death and tissue injury damage (Kehrer, 1993). A lot of studies reported the accumulation of peroxidants was associated with ...
... metabolic processes in food system or human body (Wang et al., 2006). The oxidative stress caused by free radicals and ROS would damage lipid and protein that resulted in cell death and tissue injury damage (Kehrer, 1993). A lot of studies reported the accumulation of peroxidants was associated with ...
Full text PDF
... thus resulting in the phosphorylation of serine 171 of CRTC2 and its tight association with 14-3-3 proteins in the cytosol. AMP activated protein kinase (AMPK) and its related kinases (AMPKRKs), including salt inducible kinase (SIK)1 and SIK2, are associated with the phosphorylation of this residue ...
... thus resulting in the phosphorylation of serine 171 of CRTC2 and its tight association with 14-3-3 proteins in the cytosol. AMP activated protein kinase (AMPK) and its related kinases (AMPKRKs), including salt inducible kinase (SIK)1 and SIK2, are associated with the phosphorylation of this residue ...
protease (NS34A) and an RNA polymerase
... phorylated ubiquitin as an intermediary in the PINK1–parkin pathway, the role of direct phosphorylation of parkin by PINK1 seems more complex. Koyano and colleagues report that modification of both ubiquitin and parkin at serine-65 is necessary for full activation of parkin in cells. But Kane and co ...
... phorylated ubiquitin as an intermediary in the PINK1–parkin pathway, the role of direct phosphorylation of parkin by PINK1 seems more complex. Koyano and colleagues report that modification of both ubiquitin and parkin at serine-65 is necessary for full activation of parkin in cells. But Kane and co ...
Development of an Assay for the Identification of Receptor Binding
... for identifying phage RBPs, even within sequenced genomes. This is a result of the fact that RBPs are difficult to identify based on homology, since RBPs each bind to distinct receptors. As well, phage DNA has been shown to be challenging to sequence, likely as a result of hyper-modification of the ...
... for identifying phage RBPs, even within sequenced genomes. This is a result of the fact that RBPs are difficult to identify based on homology, since RBPs each bind to distinct receptors. As well, phage DNA has been shown to be challenging to sequence, likely as a result of hyper-modification of the ...
Cardiac glycogen accumulation after dexamethasone is regulated
... signaling. Thus, glycogen accumulation that is associated with mutation of 5⬘-AMP-activated protein kinase (AMPK) has been reported to cause cardiac hypertrophy, conduction system failure, and ventricular arrhythmias (1). In glycogen storage diseases like Pompe disease, characterized by deficiency o ...
... signaling. Thus, glycogen accumulation that is associated with mutation of 5⬘-AMP-activated protein kinase (AMPK) has been reported to cause cardiac hypertrophy, conduction system failure, and ventricular arrhythmias (1). In glycogen storage diseases like Pompe disease, characterized by deficiency o ...
Vesicle formation and targeting is a multi
... “Receptor-mediated endocytosis” Example: Low-density lipoprotein (LDL), structure in which cholesterol is transported through our bodies ...
... “Receptor-mediated endocytosis” Example: Low-density lipoprotein (LDL), structure in which cholesterol is transported through our bodies ...
Slide 1 - Biskit
... VSO correlates very well with the protection of residues from itinerant water fluxes, as computed by Mihalek and colleagues (see above) which, in turn, can be considered a measure of residue activity. The calculation of shelling orders, however, is about five orders of magnitude faster than a typica ...
... VSO correlates very well with the protection of residues from itinerant water fluxes, as computed by Mihalek and colleagues (see above) which, in turn, can be considered a measure of residue activity. The calculation of shelling orders, however, is about five orders of magnitude faster than a typica ...
Two highly related regulatory subunits of PP2A exert opposite
... Xenopus embryos has opposing effects on Activin/Nodal target gene expression. (A) In situ hybridisation of gastrula-stage embryos injected with either a morpholino control (MoC) or a specific morpholino against Xenopus Bα (MoBα) or Bδ (MoBδ), or with Flag-tagged mouse Bδ mRNA (Bδ) at the one-cell st ...
... Xenopus embryos has opposing effects on Activin/Nodal target gene expression. (A) In situ hybridisation of gastrula-stage embryos injected with either a morpholino control (MoC) or a specific morpholino against Xenopus Bα (MoBα) or Bδ (MoBδ), or with Flag-tagged mouse Bδ mRNA (Bδ) at the one-cell st ...
Hitting the Sweet Spot-Glycans as Targets of Fungal Defense
... highly basic and cysteine-stabilized defensin-like proteins, AntiFungal Protein (AFP) from Aspergillus giganteus [23], Penicillium Antifungal Protein (PAF) from Penicillium chrysogenum [24], Bubble Protein (BP) from Penicillium brevicompactum Dierckx and their homologs in other ascomycetes [25]. Def ...
... highly basic and cysteine-stabilized defensin-like proteins, AntiFungal Protein (AFP) from Aspergillus giganteus [23], Penicillium Antifungal Protein (PAF) from Penicillium chrysogenum [24], Bubble Protein (BP) from Penicillium brevicompactum Dierckx and their homologs in other ascomycetes [25]. Def ...
Lecture 12
... • The IDMS technique involves the addition of a known amount of an enriched isotope of the element of interest to the sample. - made prior to sample preparation - the sample concentration can be calculated by measuring the isotope ratio of the sample and sample + spike; • Not applicable to monoisoto ...
... • The IDMS technique involves the addition of a known amount of an enriched isotope of the element of interest to the sample. - made prior to sample preparation - the sample concentration can be calculated by measuring the isotope ratio of the sample and sample + spike; • Not applicable to monoisoto ...
The Genome-linked Protein of Picornaviruses. VIII. Complete Amino
... corresponding to 22 amino acids. The aberrant migration cannot readily be explained by the primary sequence of VPg. It has been reported for aphthovirus that two or more different forms of VPg are present on RNA (King et al., 1980). These two VPgs differ in their isoelectric points and in their amin ...
... corresponding to 22 amino acids. The aberrant migration cannot readily be explained by the primary sequence of VPg. It has been reported for aphthovirus that two or more different forms of VPg are present on RNA (King et al., 1980). These two VPgs differ in their isoelectric points and in their amin ...
Delivery of a Secreted Soluble Protein to the Vacuole via a
... tonoplast. This is particularly relevant in the case of homologous proteins such as aquaporins, which are found in both membranes. Although amino acid sequence comparisons have revealed differences between the aquaporin homologs (Schaffner, 1998), it is not clear that these domains contain the targe ...
... tonoplast. This is particularly relevant in the case of homologous proteins such as aquaporins, which are found in both membranes. Although amino acid sequence comparisons have revealed differences between the aquaporin homologs (Schaffner, 1998), it is not clear that these domains contain the targe ...
Protein phosphorylation
Protein phosphorylation is a post-translational modification of proteins in which an amino acid residue is phosphorylated by a protein kinase by the addition of a covalently bound phosphate group. Phosphorylation alters the structural conformation of a protein, causing it to become activated, deactivated, or modifying its function. The reverse reaction of phosphorylation is called dephosphorylation, and is catalyzed by protein phosphatases. Protein kinases and phosphatases work independently and in a balance to regulate the function of proteins. The amino acids most commonly phosphorylated are serine, threonine, and tyrosine in eukaryotes, and histidine in prokaryotes, which play important and well-characterized roles in signaling pathways and metabolism. However, many other amino acids can also be phosphorylated, including arginine, lysine, and cysteine. Protein phosphorylation was first reported in 1906 by Phoebus Levene at the Rockefeller Institute for Medical Research with the discovery of phosphorylated vitellin. However, it was nearly 50 years until the enzymatic phosphorylation of proteins by protein kinases was discovered.