Protein Kinase C inhibits Kv1.1 potassium channel function
... min of external washing of the oocyte. Short applications of PMA were sufficient to produce this longlasting inhibition of K current [Fig. 2A; see also Fig. 6A (curve labeled ⫹C3, 16°C)], which did not recover in up to 2 h, the longest time period recorded. Over the course of hour-long recordings, K ...
... min of external washing of the oocyte. Short applications of PMA were sufficient to produce this longlasting inhibition of K current [Fig. 2A; see also Fig. 6A (curve labeled ⫹C3, 16°C)], which did not recover in up to 2 h, the longest time period recorded. Over the course of hour-long recordings, K ...
Glycogen, Amylase and tx-Glucosidase as Possible Components in
... TVo^toc filaments (20-50 g wet weight) were washed once with 0.75 M potassium phosphate buffer p H 7.0, resuspended with the help of a glass potter in fresh phosphate buffer at a density of 0.5 g wet weight/ml and were passed twice through a French press. The homogenate was centrifuged at 48,000 g f ...
... TVo^toc filaments (20-50 g wet weight) were washed once with 0.75 M potassium phosphate buffer p H 7.0, resuspended with the help of a glass potter in fresh phosphate buffer at a density of 0.5 g wet weight/ml and were passed twice through a French press. The homogenate was centrifuged at 48,000 g f ...
The MKK7 gene encodes a group of c-Jun NH2
... Mitogen-activated protein kinases (MAPKs) are components of pathways that relay signals to particular cell compartments in response to a diverse array of extracellular stimuli (38, 42, 63, 83). Activated MAPK can translocate to the nucleus and phosphorylate substrates, including transcription factor ...
... Mitogen-activated protein kinases (MAPKs) are components of pathways that relay signals to particular cell compartments in response to a diverse array of extracellular stimuli (38, 42, 63, 83). Activated MAPK can translocate to the nucleus and phosphorylate substrates, including transcription factor ...
Transport Between the Endoplasmic Reticulum and Golgi Apparatus
... 40), meaning that activated Sar1p can maintain its membrane association independently of Sec12p. It has been shown in crystallization studies that mammalian Sar1p possesses an amphipathic N-terminal domain that is exposed on binding to GTP and allows direct interaction of Sar1p with the membrane (41 ...
... 40), meaning that activated Sar1p can maintain its membrane association independently of Sec12p. It has been shown in crystallization studies that mammalian Sar1p possesses an amphipathic N-terminal domain that is exposed on binding to GTP and allows direct interaction of Sar1p with the membrane (41 ...
Assembly and disassembly of the influenza C matrix protein layer on
... is the part of the protein that binds to lipids. The same study also found that the C-terminal on the other hand binds to RNPs [6], a mechanism that is not addressed by this work. • Can M1C bind to uncharged lipids? A recent article claims that M1 from influenza A does not require any negatively cha ...
... is the part of the protein that binds to lipids. The same study also found that the C-terminal on the other hand binds to RNPs [6], a mechanism that is not addressed by this work. • Can M1C bind to uncharged lipids? A recent article claims that M1 from influenza A does not require any negatively cha ...
Interpretation of bafilomycin, pH neutralizing or protease inhibitor
... of lysosomal protease inhibitors (such as leupeptin), intralysosomal pH neutralizing agents (such as chloroquine), or drugs that inhibit the vacuolar type H+ -ATPase (v-ATPase) complex necessary for lysosomal acidification (such as bafilomycin A1). These treatments block autophagic degradation at a ...
... of lysosomal protease inhibitors (such as leupeptin), intralysosomal pH neutralizing agents (such as chloroquine), or drugs that inhibit the vacuolar type H+ -ATPase (v-ATPase) complex necessary for lysosomal acidification (such as bafilomycin A1). These treatments block autophagic degradation at a ...
What Does the Microsporidian E. cuniculi Tell Us About the Origin of
... would code for the 256 proteins that would carry out the bare essential cellular functions such as DNA replication, protein synthesis (translation and transcription), metabolism (glycolysis), and various membrane related functions. It was assumed that the minimal bacterial cell would be a minimal ce ...
... would code for the 256 proteins that would carry out the bare essential cellular functions such as DNA replication, protein synthesis (translation and transcription), metabolism (glycolysis), and various membrane related functions. It was assumed that the minimal bacterial cell would be a minimal ce ...
28. biosynthesis of proteins
... Proteins are the end products of most metabolic pathways. A typical cell requires thousands of different protein molecules at any given moment. These must be synthesized in response to the cell's current requirements, transported (or targetted) to the appropriate cellular location and ultimately deg ...
... Proteins are the end products of most metabolic pathways. A typical cell requires thousands of different protein molecules at any given moment. These must be synthesized in response to the cell's current requirements, transported (or targetted) to the appropriate cellular location and ultimately deg ...
Chlamydia effector proteins and new insights into chlamydial
... Eventually, most of the cytoplasmic space of the host cell is occupied by the inclusion and EBs exit the host cell. The mechanism of chlamydial exit from infected cells is complex with at least two pathways described, cell lysis by the activation of cysteine proteases and by extrusion of the inclusi ...
... Eventually, most of the cytoplasmic space of the host cell is occupied by the inclusion and EBs exit the host cell. The mechanism of chlamydial exit from infected cells is complex with at least two pathways described, cell lysis by the activation of cysteine proteases and by extrusion of the inclusi ...
Structure and Function of the Plasma Membrane A biochemical
... For many years cell biologists have been in general agreement that the problem of membrane structure was solved, at least to a first approximation, 257 s ...
... For many years cell biologists have been in general agreement that the problem of membrane structure was solved, at least to a first approximation, 257 s ...
+Tec - Blood Journal
... Zap-70, Syk, Tyk-2, Jak-l, and Jak-2).’ Well-shared characters among the members of the Src-family are (1) an Nterminal myristylation site: (2) a C-terminal tyrosine residue as the negative regulatory site (corresponding to Tyr-527 in c - S r ~ ) and , ~ (3) Src homology (SH) 2 and SH3 domains in th ...
... Zap-70, Syk, Tyk-2, Jak-l, and Jak-2).’ Well-shared characters among the members of the Src-family are (1) an Nterminal myristylation site: (2) a C-terminal tyrosine residue as the negative regulatory site (corresponding to Tyr-527 in c - S r ~ ) and , ~ (3) Src homology (SH) 2 and SH3 domains in th ...
Dynamic in vivo interactions among Myc network members
... c-Myc, a basic ± helix ± loop ± helix ± leucine ± zipper (bHLH ± ZIP) transcription factor, plays critical roles in proliferation, dierentiation, transformation, and apoptosis (Henriksson and LuÈscher, 1996; Facchini and Penn, 1998; Nesbit et al., 1999). c-Myc is highly expressed during proliferati ...
... c-Myc, a basic ± helix ± loop ± helix ± leucine ± zipper (bHLH ± ZIP) transcription factor, plays critical roles in proliferation, dierentiation, transformation, and apoptosis (Henriksson and LuÈscher, 1996; Facchini and Penn, 1998; Nesbit et al., 1999). c-Myc is highly expressed during proliferati ...
Structure and Function of the Plasma Membrane
... For many years cell biologists have been in general agreement that the problem of membrane structure was solved, at least to a first approximation, 257 s ...
... For many years cell biologists have been in general agreement that the problem of membrane structure was solved, at least to a first approximation, 257 s ...
Electron Transport Chain (Respiratory Chain)
... Oxidative phosphorylation a) needs proton gradient on the inner mitochondrial membrane b) is catalyzed by ATP synthase ...
... Oxidative phosphorylation a) needs proton gradient on the inner mitochondrial membrane b) is catalyzed by ATP synthase ...
RiceRBP: a resource for experimentally identified RNA
... processing based on the presence of RNA binding domains such as the RRM and KH domain (Anantharaman et al., 2002). Interestingly, review of the literature revealed that a number of proteins identified from this study categorized as having established roles in polypeptide biosynthesis or carbon metabo ...
... processing based on the presence of RNA binding domains such as the RRM and KH domain (Anantharaman et al., 2002). Interestingly, review of the literature revealed that a number of proteins identified from this study categorized as having established roles in polypeptide biosynthesis or carbon metabo ...
Evolution of an atypical de-epoxidase for photoprotection in the
... light conditions1,2. Violaxanthin de-epoxidase (VDE) is the key enzyme responsible for zeaxanthin synthesis from violaxanthin under excess light. Here we show that the Chlorophycean VDE (CVDE) gene from the model green alga Chlamydomonas reinhardtii encodes an atypical VDE. This protein is not homol ...
... light conditions1,2. Violaxanthin de-epoxidase (VDE) is the key enzyme responsible for zeaxanthin synthesis from violaxanthin under excess light. Here we show that the Chlorophycean VDE (CVDE) gene from the model green alga Chlamydomonas reinhardtii encodes an atypical VDE. This protein is not homol ...
Domain organization of human cleavage factor Im 1 Distinct
... protein has a domain organization that is reminiscent of spliceosomal SR proteins. Members of the SR family of splicing factors contain one or more N-terminal RNA recognition motifs (RRMs) that function in sequence–specific RNA binding and a Cterminal domain rich in alternating arginine and serine r ...
... protein has a domain organization that is reminiscent of spliceosomal SR proteins. Members of the SR family of splicing factors contain one or more N-terminal RNA recognition motifs (RRMs) that function in sequence–specific RNA binding and a Cterminal domain rich in alternating arginine and serine r ...
SB401, a pollen-specific protein from Solanum berthaultii
... attenuated movement of the vegetative nucleus and the generative cell from the pollen grain into the pollen tube, and disruption of the cellular polarity that is normally maintained by numerous cytoplasmic components (Åström et al., 1995; Joos et al., 1994). In addition, MTs form unique arrays and ...
... attenuated movement of the vegetative nucleus and the generative cell from the pollen grain into the pollen tube, and disruption of the cellular polarity that is normally maintained by numerous cytoplasmic components (Åström et al., 1995; Joos et al., 1994). In addition, MTs form unique arrays and ...
Cell cycle exit during terminal erythroid differentiation is associated
... Cell differentiation imparts unique identity through a coordinated tissue-specific gene expression program. This program is tightly coordinated with cell cycle exit. Commitment to cell division is regulated by a family of G1 cyclin-dependent protein kinases (CDKs) (reviewed in Morgan1 and Pavletich2 ...
... Cell differentiation imparts unique identity through a coordinated tissue-specific gene expression program. This program is tightly coordinated with cell cycle exit. Commitment to cell division is regulated by a family of G1 cyclin-dependent protein kinases (CDKs) (reviewed in Morgan1 and Pavletich2 ...
Western blot Fast purification Comparative performance
... There are articles comparing various techniques such as isolating small quantities of protein from complex samples; comparing differences in optical density analysis between spectrophotometric instruments; and presenting different methods of protein quantification. We are excited to introduce the my ...
... There are articles comparing various techniques such as isolating small quantities of protein from complex samples; comparing differences in optical density analysis between spectrophotometric instruments; and presenting different methods of protein quantification. We are excited to introduce the my ...
implications for key virulence factors in Flavobacterium columnare
... microscopy to identify the fine structures of the cells grown in liquid and on agar. We also analysed the proteins secreted extracellularly and in membrane vesicles to identify possible virulence factors. Results: Only the Rhizoid morphotype was virulent in rainbow trout. Under electron microscopy, ...
... microscopy to identify the fine structures of the cells grown in liquid and on agar. We also analysed the proteins secreted extracellularly and in membrane vesicles to identify possible virulence factors. Results: Only the Rhizoid morphotype was virulent in rainbow trout. Under electron microscopy, ...
The unfolded protein response: controlling cell fate
... by inhibiting translation (which is dependent on the protein kinase RNA-like ER kinase (PERK)-mediated phosphorylation of eukaryotic translation initiator factor 2α (eIF2α)), activating mRNA decay by regulated inositol-requiring protein 1 (IRE1)dependent decay (RIDD), and activating autophagy throug ...
... by inhibiting translation (which is dependent on the protein kinase RNA-like ER kinase (PERK)-mediated phosphorylation of eukaryotic translation initiator factor 2α (eIF2α)), activating mRNA decay by regulated inositol-requiring protein 1 (IRE1)dependent decay (RIDD), and activating autophagy throug ...
Cell signalling - The Open University
... the signal can be amplified (or damped down) as it travels along the signalling pathway; it can switch on multiple pathways, leading to several cellular responses in diverse regions of the cell; information can be processed from several different receptors at once to produce an integrated resp ...
... the signal can be amplified (or damped down) as it travels along the signalling pathway; it can switch on multiple pathways, leading to several cellular responses in diverse regions of the cell; information can be processed from several different receptors at once to produce an integrated resp ...
Leukaemia Section Anaplastic large cell lymphoma (ALCL) Atlas of Genetics and Cytogenetics
... sometimes called ALK lymphomas, or ALKomas. ALK+ ALCL can be further divided into t(2;5) cases, with NPM1-ALK fusion protein which localises both in the cytoplasm and in the nucleus, and t(2;Var), involving various partners and ALK, and a cytoplasmic localization of the fusion protein; the latter ar ...
... sometimes called ALK lymphomas, or ALKomas. ALK+ ALCL can be further divided into t(2;5) cases, with NPM1-ALK fusion protein which localises both in the cytoplasm and in the nucleus, and t(2;Var), involving various partners and ALK, and a cytoplasmic localization of the fusion protein; the latter ar ...
Protein phosphorylation
Protein phosphorylation is a post-translational modification of proteins in which an amino acid residue is phosphorylated by a protein kinase by the addition of a covalently bound phosphate group. Phosphorylation alters the structural conformation of a protein, causing it to become activated, deactivated, or modifying its function. The reverse reaction of phosphorylation is called dephosphorylation, and is catalyzed by protein phosphatases. Protein kinases and phosphatases work independently and in a balance to regulate the function of proteins. The amino acids most commonly phosphorylated are serine, threonine, and tyrosine in eukaryotes, and histidine in prokaryotes, which play important and well-characterized roles in signaling pathways and metabolism. However, many other amino acids can also be phosphorylated, including arginine, lysine, and cysteine. Protein phosphorylation was first reported in 1906 by Phoebus Levene at the Rockefeller Institute for Medical Research with the discovery of phosphorylated vitellin. However, it was nearly 50 years until the enzymatic phosphorylation of proteins by protein kinases was discovered.