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... (BSA)];33 % was considered surface bound (accessible to BSA). The cellular distribution of aminopeptidase activity is consistent with the model that cell surface aminopeptidase generate oligomers which diffuse into the periplasm where they are monomerized by the high aminopeptidase activity in an en ...
... (BSA)];33 % was considered surface bound (accessible to BSA). The cellular distribution of aminopeptidase activity is consistent with the model that cell surface aminopeptidase generate oligomers which diffuse into the periplasm where they are monomerized by the high aminopeptidase activity in an en ...
Leukaemia Section Anaplastic large cell lymphoma (ALCL) Atlas of Genetics and Cytogenetics
... sometimes called ALK lymphomas, or ALKomas. ALK+ ALCL can be further divided into t(2;5) cases, with NPM1-ALK fusion protein which localises both in the cytoplasm and in the nucleus, and t(2;Var), involving various partners and ALK, and a cytoplasmic localization of the fusion protein; the latter ar ...
... sometimes called ALK lymphomas, or ALKomas. ALK+ ALCL can be further divided into t(2;5) cases, with NPM1-ALK fusion protein which localises both in the cytoplasm and in the nucleus, and t(2;Var), involving various partners and ALK, and a cytoplasmic localization of the fusion protein; the latter ar ...
A Cyanobacterial Chlorophyll Synthase-HliD
... The photosystem I (PSI) and photosystem II (PSII) chlorophyllprotein complexes of cyanobacteria and plants absorb solar energy and use it for charge separation, which drives downstream processes such as NADPH formation and ATP synthesis. The structures of these complexes (Jordan et al., 2001; Umena ...
... The photosystem I (PSI) and photosystem II (PSII) chlorophyllprotein complexes of cyanobacteria and plants absorb solar energy and use it for charge separation, which drives downstream processes such as NADPH formation and ATP synthesis. The structures of these complexes (Jordan et al., 2001; Umena ...
Observations of green fluorescent protein as a fusion partner in
... wherein the total yield and specific activity of the productportion of the fusion were decreased while the time course profiles were similar. Thus, the advantages in processing (upstream and downstream) which have been projected for the use of the GFP fusions (Albano et al., 1996; Poppenborg et al., ...
... wherein the total yield and specific activity of the productportion of the fusion were decreased while the time course profiles were similar. Thus, the advantages in processing (upstream and downstream) which have been projected for the use of the GFP fusions (Albano et al., 1996; Poppenborg et al., ...
Anti-OPN Monoclonal Antibodies as Probes of OPN Structure and
... ABSTRACT: OPN, a primarily secreted protein found in all body fluids, is post-translationally modified; there is also an intracellular form. It is functionally important in bone remodeling, the progression of autoimmune disease, cancer metastasis, inflammatory processes and stress responses. Our res ...
... ABSTRACT: OPN, a primarily secreted protein found in all body fluids, is post-translationally modified; there is also an intracellular form. It is functionally important in bone remodeling, the progression of autoimmune disease, cancer metastasis, inflammatory processes and stress responses. Our res ...
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... acid residues [33]. The small HPCopP is homologous to CopZ, encoded by the E. hirae and B. subtilis cop operons. CopZ proteins have a conserved structure, β-α-β-β-α-β with a similar metal binding region. However, the structure of HPCopP has a β-α-β-β-α fold (Figure 3A). The helices correspond to res ...
... acid residues [33]. The small HPCopP is homologous to CopZ, encoded by the E. hirae and B. subtilis cop operons. CopZ proteins have a conserved structure, β-α-β-β-α-β with a similar metal binding region. However, the structure of HPCopP has a β-α-β-β-α fold (Figure 3A). The helices correspond to res ...
Alpha-COPI Coatomer Protein Is Required for Rough Endoplasmic
... and number of RER whorls in midgut epithelial cells were found to decrease significantly after feeding, and several KDELcontaining proteins were shown to have altered expression levels. LC-MS/MS mass spectrometry was used to analyze midgut microsomal proteins isolated from unfed and amino acid fed m ...
... and number of RER whorls in midgut epithelial cells were found to decrease significantly after feeding, and several KDELcontaining proteins were shown to have altered expression levels. LC-MS/MS mass spectrometry was used to analyze midgut microsomal proteins isolated from unfed and amino acid fed m ...
Leukaemia Section Anaplasic large cell lymphoma (ALCL) Atlas of Genetics and Cytogenetics
... sometimes called ALK lymphomas, or ALKomas. ALK+ ALCL can be further divided into t(2;5) cases, with NPM1-ALK fusion protein which localises both in the cytoplasm and in the nucleus, and t(2;Var), involving various partners and ALK, and a cytoplasmic localization of the fusion protein; the latter ar ...
... sometimes called ALK lymphomas, or ALKomas. ALK+ ALCL can be further divided into t(2;5) cases, with NPM1-ALK fusion protein which localises both in the cytoplasm and in the nucleus, and t(2;Var), involving various partners and ALK, and a cytoplasmic localization of the fusion protein; the latter ar ...
Expression of target protein and Affibody molecule in Pichia pastoris
... The target protein EpCAM (Epidermal Cell Adhesion Molecule), also known as 17-1A antigen, is a human cancer antigen displayed in excess on the tumour cell surface of many common cancers such as colon, lung and breast cancers (Balzar et al., 1999). A specific binder towards the antigen would be able ...
... The target protein EpCAM (Epidermal Cell Adhesion Molecule), also known as 17-1A antigen, is a human cancer antigen displayed in excess on the tumour cell surface of many common cancers such as colon, lung and breast cancers (Balzar et al., 1999). A specific binder towards the antigen would be able ...
Domain structure of a human general
... same extent as the wild type clone, but further deletion of the C-terminal sequence up to die 128th residue resulted in a complete loss of the CAT activity (lane 5). By contrast, transfection with clones encoding various regions of C-terminal (lanes 6 and 7) or internal sequences (lane 8,9 and 10) d ...
... same extent as the wild type clone, but further deletion of the C-terminal sequence up to die 128th residue resulted in a complete loss of the CAT activity (lane 5). By contrast, transfection with clones encoding various regions of C-terminal (lanes 6 and 7) or internal sequences (lane 8,9 and 10) d ...
A Rab4-like GTPase in Dictyostelium discoideum
... Colocalization of V-H+-ATPase and a Rab4-like GTPase 2803 minutes at 10,000 g) and high-speed (60 minutes at 100,000 g) supernatants and pellets. The resulting pellets designated P1, P2 and P3, as well as the supernatants designated S1, S2 and S3, were subjected to SDS-PAGE and western blot analysi ...
... Colocalization of V-H+-ATPase and a Rab4-like GTPase 2803 minutes at 10,000 g) and high-speed (60 minutes at 100,000 g) supernatants and pellets. The resulting pellets designated P1, P2 and P3, as well as the supernatants designated S1, S2 and S3, were subjected to SDS-PAGE and western blot analysi ...
A Difference between Rods and Cones in the Renewal of Outer
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The src
... exhibit various cellular alterations v-src affects a wide variety of cellular targets The first BIG step: using serum to immunoprecipitate the v-Src protein ...
... exhibit various cellular alterations v-src affects a wide variety of cellular targets The first BIG step: using serum to immunoprecipitate the v-Src protein ...
of Pseudomonas aeruginosa - BMC Microbiology
... generating clinical infection episodes which compromise the host’s defence mechanisms [1]. Infections with Pseudomonas aeruginosa represent a major therapeutic challenge in which choice of the right antibiotic is fundamental, however this choice is complicated by the fact that P. aeruginosa presents ...
... generating clinical infection episodes which compromise the host’s defence mechanisms [1]. Infections with Pseudomonas aeruginosa represent a major therapeutic challenge in which choice of the right antibiotic is fundamental, however this choice is complicated by the fact that P. aeruginosa presents ...
Allosteric regulation and catalysis emerge via a common
... Allostery can be defined as the regulation of protein function, structure and/or flexibility induced by the binding of a ligand or another protein, termed an effector, at a site away from the active site, also referred to as the allosteric site. Those effectors that increase a particular protein fun ...
... Allostery can be defined as the regulation of protein function, structure and/or flexibility induced by the binding of a ligand or another protein, termed an effector, at a site away from the active site, also referred to as the allosteric site. Those effectors that increase a particular protein fun ...
Cofradionation of HeLa Cell Replication Proteins With Ors
... useful in the biochemical analysis of the proteins involved in the replication process. Fractionation of human cell extracts has identified several proteins that are necessary for complete in vitro SV40 DNA replication, which are believed to participate in the same process in the cell. Reconstitutio ...
... useful in the biochemical analysis of the proteins involved in the replication process. Fractionation of human cell extracts has identified several proteins that are necessary for complete in vitro SV40 DNA replication, which are believed to participate in the same process in the cell. Reconstitutio ...
Translocation of proteins across archaeal cytoplasmic membranes
... been identified to be involved in targeting post-translationally translocated proteins to the Sec pore and maintaining their translocation competent states. In E. coli, the cytoplasmic protein SecB binds preproteins as a homotetramer of 16 kDa subunits [38] and maintains the proteins in an unfolded c ...
... been identified to be involved in targeting post-translationally translocated proteins to the Sec pore and maintaining their translocation competent states. In E. coli, the cytoplasmic protein SecB binds preproteins as a homotetramer of 16 kDa subunits [38] and maintains the proteins in an unfolded c ...
Agrobacterium tumefaciens and A. rhizogenes use different proteins
... express rol (root loci) genes transferred from the rootinducing (Ri) plasmid (White et al., 1985). In contrast, A. tumefaciens causes unorganized growth of infected plant cells. Oncogenes transferred from the tumour-inducing (Ti) plasmid into plant cells encode proteins involved in synthesis of plan ...
... express rol (root loci) genes transferred from the rootinducing (Ri) plasmid (White et al., 1985). In contrast, A. tumefaciens causes unorganized growth of infected plant cells. Oncogenes transferred from the tumour-inducing (Ti) plasmid into plant cells encode proteins involved in synthesis of plan ...
2.2 Membrane Structure and Functions
... molecules that make up the membrane, as well as the temperature. • Membranes contain sterols, which help to maintain their fluidity. • Membrane proteins have four functions: transport, enzymatic activity, triggering signals, and attachment and recognition of molecules. • Membrane proteins may be ...
... molecules that make up the membrane, as well as the temperature. • Membranes contain sterols, which help to maintain their fluidity. • Membrane proteins have four functions: transport, enzymatic activity, triggering signals, and attachment and recognition of molecules. • Membrane proteins may be ...
An ADP/ATP-Specific Mitochondrial Carrier Protein
... Rosetta2 cells imported only trace amounts of labeled nucleotides over the same time frame (Fig. 4a and b). To assess whether other nucleotides are also transported by the A. locustae MCF protein, substrate specificity of the recombinant protein was analyzed by competition. Import of radioactively l ...
... Rosetta2 cells imported only trace amounts of labeled nucleotides over the same time frame (Fig. 4a and b). To assess whether other nucleotides are also transported by the A. locustae MCF protein, substrate specificity of the recombinant protein was analyzed by competition. Import of radioactively l ...
Journal of Cell Biology - Institute of Cancer Research Repository
... Figure 1. Apoptotic nuclear breakdown is blocked following ROCK inhibition. (A) Transmission electron micrograph of an NIH 3T3 fibroblast treated with 25 ng/ml TNF plus 10 g/ml cycloheximide (CHX) for 2 h to induce apoptosis, showing large plasma membrane blebs, nuclear disintegration, and heteroc ...
... Figure 1. Apoptotic nuclear breakdown is blocked following ROCK inhibition. (A) Transmission electron micrograph of an NIH 3T3 fibroblast treated with 25 ng/ml TNF plus 10 g/ml cycloheximide (CHX) for 2 h to induce apoptosis, showing large plasma membrane blebs, nuclear disintegration, and heteroc ...
Different subcellular localisations of TRIM22 suggest species
... to allow for expression of AcGFP-tagged proteins, respectively. Protein expression was visualised 48 h posttransfection by confocal microscopy. Interestingly, human and rhesus macaque TRIM22 showed indeed a different subcellular localisation (Fig. 1a). Human TRIM22 was localised in diffuse accumulat ...
... to allow for expression of AcGFP-tagged proteins, respectively. Protein expression was visualised 48 h posttransfection by confocal microscopy. Interestingly, human and rhesus macaque TRIM22 showed indeed a different subcellular localisation (Fig. 1a). Human TRIM22 was localised in diffuse accumulat ...
MICROSTRUCTURE OF PROTEIN BODIES IN MARAMA BEAN SPECIES
... of spherical globoids from many legumes showed that they are rich sources of P, K, Ca and Mg (Lott and Buttrose, 1978; Lott, 1981). These minerals were found in high concentration in marama beans (Table 3), probably originated mainly from the globoid sites. Most soya protein bodies did not contain g ...
... of spherical globoids from many legumes showed that they are rich sources of P, K, Ca and Mg (Lott and Buttrose, 1978; Lott, 1981). These minerals were found in high concentration in marama beans (Table 3), probably originated mainly from the globoid sites. Most soya protein bodies did not contain g ...
Proteomic Strategies to Analyze Cell
... The use of Proteomic strategies in the discovery process is imperative since post-transcriptional modification can produce dramatic changes in protein levels and activity that are invisible to DNA arrays. The introduction of new and improved proteomics solutions with increased sensitivity, specifici ...
... The use of Proteomic strategies in the discovery process is imperative since post-transcriptional modification can produce dramatic changes in protein levels and activity that are invisible to DNA arrays. The introduction of new and improved proteomics solutions with increased sensitivity, specifici ...
The proteome of the infectious bronchitis virus Beau
... proteins were detected in mock-infected allantoic fluid. To compare whether there were any changes in the groups of proteins that could be identified between uninfected and mock-infected allantoic fluid, lists of proteins identified in each sample were analysed using the PANTHER classification syste ...
... proteins were detected in mock-infected allantoic fluid. To compare whether there were any changes in the groups of proteins that could be identified between uninfected and mock-infected allantoic fluid, lists of proteins identified in each sample were analysed using the PANTHER classification syste ...
Protein phosphorylation
Protein phosphorylation is a post-translational modification of proteins in which an amino acid residue is phosphorylated by a protein kinase by the addition of a covalently bound phosphate group. Phosphorylation alters the structural conformation of a protein, causing it to become activated, deactivated, or modifying its function. The reverse reaction of phosphorylation is called dephosphorylation, and is catalyzed by protein phosphatases. Protein kinases and phosphatases work independently and in a balance to regulate the function of proteins. The amino acids most commonly phosphorylated are serine, threonine, and tyrosine in eukaryotes, and histidine in prokaryotes, which play important and well-characterized roles in signaling pathways and metabolism. However, many other amino acids can also be phosphorylated, including arginine, lysine, and cysteine. Protein phosphorylation was first reported in 1906 by Phoebus Levene at the Rockefeller Institute for Medical Research with the discovery of phosphorylated vitellin. However, it was nearly 50 years until the enzymatic phosphorylation of proteins by protein kinases was discovered.