ZO-3, a Novel Member of the MAGUK Protein Family Found at the
... boundary between the compositionally distinct apical and basolateral plasma membrane domains of epithelial and endothelial cells. The molecular configuration of the tight junction has generated considerable interest in the last decade. Actin filaments (17, 29) and the peripheral membrane proteins ZO ...
... boundary between the compositionally distinct apical and basolateral plasma membrane domains of epithelial and endothelial cells. The molecular configuration of the tight junction has generated considerable interest in the last decade. Actin filaments (17, 29) and the peripheral membrane proteins ZO ...
GPCR–G fusion proteins
... by Bertin et al.1 have been confirmed by Seifert and colleagues. Moreover, they found that there is highly efficient ligand-regulation of GTPgS binding and GTPase activity in Sf9 membranes expressing b2AR-GsaL (Ref. 5). In contrast, detection of ligand-regulation of GTPase and GTPgS binding by non-f ...
... by Bertin et al.1 have been confirmed by Seifert and colleagues. Moreover, they found that there is highly efficient ligand-regulation of GTPgS binding and GTPase activity in Sf9 membranes expressing b2AR-GsaL (Ref. 5). In contrast, detection of ligand-regulation of GTPase and GTPgS binding by non-f ...
Identification of Pexl3p, a Peroxisomal Membrane Receptor for the
... are imported posttranslationally (for review see Lazarow and Fujiki, 1985). Import of peroxisomal matrix proteins requires both ATP and cytosolic factors (Imanaka et al., 1987; Wendland and Subramani, 1993), and recent evidence suggests that peroxisomal proteins can be imported in a folded state (Mc ...
... are imported posttranslationally (for review see Lazarow and Fujiki, 1985). Import of peroxisomal matrix proteins requires both ATP and cytosolic factors (Imanaka et al., 1987; Wendland and Subramani, 1993), and recent evidence suggests that peroxisomal proteins can be imported in a folded state (Mc ...
Studies on legume receptors for Nod and Myc symbiotic signals
... large number of plant viruses and other pathogens such as bacteria, oomycetes and fungi and thus is widely used in plant virology and for host–pathogen interaction studies. For the reason that its large leaves can be easily transformed with high efficiency with Agrobacterium tumefaciens, N. benthami ...
... large number of plant viruses and other pathogens such as bacteria, oomycetes and fungi and thus is widely used in plant virology and for host–pathogen interaction studies. For the reason that its large leaves can be easily transformed with high efficiency with Agrobacterium tumefaciens, N. benthami ...
Engagement of the inhibitory receptor CD158a
... CD3-redirected lysis of murine FcR+ P815 cells. Thus, TCR and KIR were triggered on CTL by microbeads coated with anti-CD3 or anti-CD3 + anti-CD158a mAbs and membrane reorganization was analysed using confocal microscopy (Fig 2B). Stimulation by anti-CD3 coated beads (beads exhibit light red autoflu ...
... CD3-redirected lysis of murine FcR+ P815 cells. Thus, TCR and KIR were triggered on CTL by microbeads coated with anti-CD3 or anti-CD3 + anti-CD158a mAbs and membrane reorganization was analysed using confocal microscopy (Fig 2B). Stimulation by anti-CD3 coated beads (beads exhibit light red autoflu ...
Transcript - UAB School of Optometry
... i. The amino acids join the nitrogen to the carbon in a head-to-tail formation. ii. The peptide bond releases water when formed, a reaction called hydrolysis. 1. This reaction needs energy because the spontaneous and favorable reaction goes in the other direction to split the two amino acids. iii. T ...
... i. The amino acids join the nitrogen to the carbon in a head-to-tail formation. ii. The peptide bond releases water when formed, a reaction called hydrolysis. 1. This reaction needs energy because the spontaneous and favorable reaction goes in the other direction to split the two amino acids. iii. T ...
REFLECTIONS
... for acetyl-LDL. Other negatively charged macromolecules, including maleyl-LDL, maleyl-albumin, sulfated polysaccharides (such as fucoidan and dextran sulfate), and polynucleotides (such as poly(I) and poly(G)) competed for acetyl-LDL uptake. There was some selectivity, however. Certain negatively ch ...
... for acetyl-LDL. Other negatively charged macromolecules, including maleyl-LDL, maleyl-albumin, sulfated polysaccharides (such as fucoidan and dextran sulfate), and polynucleotides (such as poly(I) and poly(G)) competed for acetyl-LDL uptake. There was some selectivity, however. Certain negatively ch ...
Truncated Photosystem Chlorophyll Antenna Size in the Green
... antenna of both photosystems, it can be estimated that the size of the light-harvesting antenna of the photosystems in the tla3 mutant is about one-third of that in the wild type. This observation suggests that there is a substantially smaller Chl antenna size of the photosystems in the mutant. When ...
... antenna of both photosystems, it can be estimated that the size of the light-harvesting antenna of the photosystems in the tla3 mutant is about one-third of that in the wild type. This observation suggests that there is a substantially smaller Chl antenna size of the photosystems in the mutant. When ...
Natural Genetic Variation Modifies Gene Expression
... protein-coding genes. Yet in order for this variation to affect higher order traits it must lead to differences at the protein level. Variation in protein-specific processes including protein synthesis rates and protein decay rates could amplify, mask, or even reverse effects transmitted from the tr ...
... protein-coding genes. Yet in order for this variation to affect higher order traits it must lead to differences at the protein level. Variation in protein-specific processes including protein synthesis rates and protein decay rates could amplify, mask, or even reverse effects transmitted from the tr ...
PDF - Blood Journal
... total protein and phospholipid content.4 Similarly, in mechanically induced vesiculation of erythrocytes, the specific enrichment of GPI-linked CD59 in the vesicles has been shown.16 Interestingly, red cells of patients with paroxysmal nocturnal hemoglobinuria, which lack GPI-linked proteins, have a ...
... total protein and phospholipid content.4 Similarly, in mechanically induced vesiculation of erythrocytes, the specific enrichment of GPI-linked CD59 in the vesicles has been shown.16 Interestingly, red cells of patients with paroxysmal nocturnal hemoglobinuria, which lack GPI-linked proteins, have a ...
The distribution of SMN protein complex in human fetal tissues and
... of which has hitherto been unclear. Here, we present immunoblot analyses showing that normal SMN protein expression undergoes a marked decay in the postnatal period compared with fetal development. Morphological and immunohistochemical analyses of the SMN protein in human fetal tissues showed a gene ...
... of which has hitherto been unclear. Here, we present immunoblot analyses showing that normal SMN protein expression undergoes a marked decay in the postnatal period compared with fetal development. Morphological and immunohistochemical analyses of the SMN protein in human fetal tissues showed a gene ...
You can answer the question Yourself with a few
... including it in your daily regimen. It’s enormously advantageous for several reasons, including amino acid and microfraction content (more on that in a minute) and digestion rate. Whey is one of the richest sources of ...
... including it in your daily regimen. It’s enormously advantageous for several reasons, including amino acid and microfraction content (more on that in a minute) and digestion rate. Whey is one of the richest sources of ...
A Patch of Surface-Exposed Residues Mediates
... Protein kinases are frequent points of control in diverse signaling pathways, and their structure and enzymology are well understood (Huse and Kuriyan, 2002). There are four kinase substructures that control ATP binding and orientation, binding of the peptide substrate, and catalytic phosphotransfer ...
... Protein kinases are frequent points of control in diverse signaling pathways, and their structure and enzymology are well understood (Huse and Kuriyan, 2002). There are four kinase substructures that control ATP binding and orientation, binding of the peptide substrate, and catalytic phosphotransfer ...
Huntingtin: Its Role in Gene Expression
... of psychiatric symptoms, typically depression and anxiety, but other symptoms such as obsession, compulsion and irritability can also occur. Another hallmark of Huntington’s disease is the development of involuntary and unwanted movements in early stages of the disease. Clinical diagnosis occurs by ...
... of psychiatric symptoms, typically depression and anxiety, but other symptoms such as obsession, compulsion and irritability can also occur. Another hallmark of Huntington’s disease is the development of involuntary and unwanted movements in early stages of the disease. Clinical diagnosis occurs by ...
Highly conserved epitope domain in major core protein p24 is
... antiserum had no significant reactivity with anothffr region from the epitope (Fig. 2d). These results suggest that the site could be an immunodominant epitope in mice. Recent studies have revealed that the major neutralization antigenic sites of foot-and-mouth disease virus (Francis et al., 1987) a ...
... antiserum had no significant reactivity with anothffr region from the epitope (Fig. 2d). These results suggest that the site could be an immunodominant epitope in mice. Recent studies have revealed that the major neutralization antigenic sites of foot-and-mouth disease virus (Francis et al., 1987) a ...
Calcium, a signaling molecule in the endoplasmic reticulum?
... below this level, it is unlikely that Ca21 binding to the high-affinity Ca21 binding, proline-rich domain of calreticulin and calnexin regulate these protein–carbohydrate interactions (Fig. 1). It is not clear at present which Ca21-binding site is essential for the lectin-like activity of calreticul ...
... below this level, it is unlikely that Ca21 binding to the high-affinity Ca21 binding, proline-rich domain of calreticulin and calnexin regulate these protein–carbohydrate interactions (Fig. 1). It is not clear at present which Ca21-binding site is essential for the lectin-like activity of calreticul ...
Processing Synthesis of Cellulase from Ripening
... Mann Laboratory, Department of Vegetable Crops, University of California, Davis, California 95616 ing secreted proteins to the correct cellular compartment (17). Studies of intracellular transport of legume storage proteins have The biosynthesis and processing of cellulase from ripening avocado demo ...
... Mann Laboratory, Department of Vegetable Crops, University of California, Davis, California 95616 ing secreted proteins to the correct cellular compartment (17). Studies of intracellular transport of legume storage proteins have The biosynthesis and processing of cellulase from ripening avocado demo ...
Dynamic properties of a reconstituted myelin sheath
... and correlations have been observed between myelin structural modifications [13,22] and some common neurological degenerative events of pathological nature in humans. In this context, wide angle X-ray diffraction studies [5] revealed that the lipid phase transition temperature of MS myelin is 20◦ C ...
... and correlations have been observed between myelin structural modifications [13,22] and some common neurological degenerative events of pathological nature in humans. In this context, wide angle X-ray diffraction studies [5] revealed that the lipid phase transition temperature of MS myelin is 20◦ C ...
In vivo characterization of the properties of SUMO1
... SUMOylation in vivo. For example, expression of an artificial SIM peptide has been shown to decrease protein–protein interactions necessary for DNA damage repair [17]. However, most SIM peptides do not discriminate between different SUMO isoforms and bind with low affinity [17,18]. A significant adv ...
... SUMOylation in vivo. For example, expression of an artificial SIM peptide has been shown to decrease protein–protein interactions necessary for DNA damage repair [17]. However, most SIM peptides do not discriminate between different SUMO isoforms and bind with low affinity [17,18]. A significant adv ...
Phloem RNA-binding proteins as potential components of the long
... phloem protein (CmPP16), a RBP with immunological crossreactivity and a functional similarity to the MP of the Red clover necrotic mosaic virus (RCNMV; Xoconostle-Cazares et al., 1999). R-CmPP16-1 was seen to bind both sense and antisense CmPP161 RNA, as well as RCNMV RNA2, but failed to interact wi ...
... phloem protein (CmPP16), a RBP with immunological crossreactivity and a functional similarity to the MP of the Red clover necrotic mosaic virus (RCNMV; Xoconostle-Cazares et al., 1999). R-CmPP16-1 was seen to bind both sense and antisense CmPP161 RNA, as well as RCNMV RNA2, but failed to interact wi ...
THE PLANT CELL CYCLE Walter Dewitte and James A.H. Murray
... GENERAL PRINCIPLES OF CELL-CYCLE CONTROL The eukaryotic cell cycle is regulated at multiple points, but all or most of these involve the activation of a special class of serine-threonine protein kinases, which are functionally defined as requiring binding for activity to a regulatory protein known a ...
... GENERAL PRINCIPLES OF CELL-CYCLE CONTROL The eukaryotic cell cycle is regulated at multiple points, but all or most of these involve the activation of a special class of serine-threonine protein kinases, which are functionally defined as requiring binding for activity to a regulatory protein known a ...
Universal Features of Post-Transcriptional Gene Regulation Are
... includes 16 major factors, including proteins with homologies to components of metazoan P granules and archaeal proteins. Containing translationally silent transcripts, this mRNP integrates eIF4E and poly(A)-binding protein but excludes P body RNA degradation factors and translation-initiation promo ...
... includes 16 major factors, including proteins with homologies to components of metazoan P granules and archaeal proteins. Containing translationally silent transcripts, this mRNP integrates eIF4E and poly(A)-binding protein but excludes P body RNA degradation factors and translation-initiation promo ...
12 Insights into the mechanisms underlying CFTR channel activity
... disordered region of the protein which undergoes dynamic interactions with other CFTR domains [40,41]. Although this domain is disordered, it possesses regions with the propensity to form α‑helices in the non‑phosphorylated state and PKA phosphorylation reduces this propensity. These findings are co ...
... disordered region of the protein which undergoes dynamic interactions with other CFTR domains [40,41]. Although this domain is disordered, it possesses regions with the propensity to form α‑helices in the non‑phosphorylated state and PKA phosphorylation reduces this propensity. These findings are co ...
Assembly of AO and DHAS - Journal of Cell Science
... the AO and DHAS from the pellet (Fig. 1C) and no the only species that crosses the peroxisomal membrane. peroxisomal membrane (Fig. 1D). In summary, there is little Having shown that AO is imported as a monomer and DHAS cross contamination between cytosol (in the SUP), matrix and as a dimer, we next ...
... the AO and DHAS from the pellet (Fig. 1C) and no the only species that crosses the peroxisomal membrane. peroxisomal membrane (Fig. 1D). In summary, there is little Having shown that AO is imported as a monomer and DHAS cross contamination between cytosol (in the SUP), matrix and as a dimer, we next ...
TIBS Review - Structural Genomics Consortium
... progression [36]. In HeLa cells BRD2 has yet another function; it was found to affect alternative splicing of multiple genes without affecting RNA polymerase II processivity downstream of the alternatively spliced elements. The affected genes included some involved in MAPK signalling and the respons ...
... progression [36]. In HeLa cells BRD2 has yet another function; it was found to affect alternative splicing of multiple genes without affecting RNA polymerase II processivity downstream of the alternatively spliced elements. The affected genes included some involved in MAPK signalling and the respons ...
Protein phosphorylation
Protein phosphorylation is a post-translational modification of proteins in which an amino acid residue is phosphorylated by a protein kinase by the addition of a covalently bound phosphate group. Phosphorylation alters the structural conformation of a protein, causing it to become activated, deactivated, or modifying its function. The reverse reaction of phosphorylation is called dephosphorylation, and is catalyzed by protein phosphatases. Protein kinases and phosphatases work independently and in a balance to regulate the function of proteins. The amino acids most commonly phosphorylated are serine, threonine, and tyrosine in eukaryotes, and histidine in prokaryotes, which play important and well-characterized roles in signaling pathways and metabolism. However, many other amino acids can also be phosphorylated, including arginine, lysine, and cysteine. Protein phosphorylation was first reported in 1906 by Phoebus Levene at the Rockefeller Institute for Medical Research with the discovery of phosphorylated vitellin. However, it was nearly 50 years until the enzymatic phosphorylation of proteins by protein kinases was discovered.