- Wiley Online Library
... The Concise Guide to PHARMACOLOGY 2015/16 provides concise overviews of the key properties of over 1750 human drug targets with their pharmacology, plus links to an open access knowledgebase of drug targets and their ligands (www.guidetopharmacology.org), which provides more detailed views of target ...
... The Concise Guide to PHARMACOLOGY 2015/16 provides concise overviews of the key properties of over 1750 human drug targets with their pharmacology, plus links to an open access knowledgebase of drug targets and their ligands (www.guidetopharmacology.org), which provides more detailed views of target ...
Identification of Hedgehog Pathway Components by RNAi in
... Classical genetic screens can be limited by the selectivity of mutational targeting, the complexities of anatomically based phenotypic analysis, or difficulties in subsequent gene identification. Focusing on signaling response to the secreted morphogen Hedgehog (Hh), we used RNA interference (RNAi) an ...
... Classical genetic screens can be limited by the selectivity of mutational targeting, the complexities of anatomically based phenotypic analysis, or difficulties in subsequent gene identification. Focusing on signaling response to the secreted morphogen Hedgehog (Hh), we used RNA interference (RNAi) an ...
Supplementary information
... and non-specific manner, while ECFP-EYFP protein is only subject to molecular sieving effect, resulting from relatively cluttered nuclear environment. High mobility was also expected in the case of p53 L344P (Sup. 1A), reflecting its mostly monomeric quaternary structure as compared to the other tes ...
... and non-specific manner, while ECFP-EYFP protein is only subject to molecular sieving effect, resulting from relatively cluttered nuclear environment. High mobility was also expected in the case of p53 L344P (Sup. 1A), reflecting its mostly monomeric quaternary structure as compared to the other tes ...
Purification, Identification and Characterisation of - DORAS
... homology with, and related type II trans-membrane prolyl serine peptidases, which are inducible, specific for proline-containing peptides and macromolecules and exert their mechanisms of action on the cell surface. DPPIV and Seprase exhibit multiple functions due to their abilities to form complexes ...
... homology with, and related type II trans-membrane prolyl serine peptidases, which are inducible, specific for proline-containing peptides and macromolecules and exert their mechanisms of action on the cell surface. DPPIV and Seprase exhibit multiple functions due to their abilities to form complexes ...
Proteomics insights: proteins related to larval attachment and
... The transition in an animal from a pelagic larval stage to a sessile benthic juvenile typically requires major morphological and behavioral changes. Larval competency, attachment and initiation of metamorphosis are thought to be regulated by intrinsic chemical signals and specific sets of proteins. ...
... The transition in an animal from a pelagic larval stage to a sessile benthic juvenile typically requires major morphological and behavioral changes. Larval competency, attachment and initiation of metamorphosis are thought to be regulated by intrinsic chemical signals and specific sets of proteins. ...
Chemistry in living systems
... Rideout and coworkers recognized the potential use of ketones and aldehydes for chemoselective drug assembly in the presence of living cells53, 54, 55. They reported that decanal and octyl aminoguanidine—both independently harmless to cells—react selectively to form a hydrazone-linked detergent cap ...
... Rideout and coworkers recognized the potential use of ketones and aldehydes for chemoselective drug assembly in the presence of living cells53, 54, 55. They reported that decanal and octyl aminoguanidine—both independently harmless to cells—react selectively to form a hydrazone-linked detergent cap ...
Communication - Department of Molecular Physiology and Biophysics
... binds laminin and dystrophin binds to actin filaments, indicating that one function of the dystrophin-glycoprotein complex is to provide a link between the extracellular matrix and the actin cytoskeleton (5, 6). 59-DAP (now named syntrophin) is a 59kDa cytoplasmic protein triplet in the purified dys ...
... binds laminin and dystrophin binds to actin filaments, indicating that one function of the dystrophin-glycoprotein complex is to provide a link between the extracellular matrix and the actin cytoskeleton (5, 6). 59-DAP (now named syntrophin) is a 59kDa cytoplasmic protein triplet in the purified dys ...
Midbodies and phragmoplasts: analogous structures
... Cytokinesis is an event common to all organisms that involves the precise coordination of independent pathways involved in cell-cycle regulation and microtubule, membrane, actin and organelle dynamics. In animal cells, the spindle midzone/midbody with associated endo-membrane system are required for ...
... Cytokinesis is an event common to all organisms that involves the precise coordination of independent pathways involved in cell-cycle regulation and microtubule, membrane, actin and organelle dynamics. In animal cells, the spindle midzone/midbody with associated endo-membrane system are required for ...
Abstract
... Cytosolic Ca2+ plays an important role in intracellular signaling. Na+/Ca2+ exchanger (NCX) is a plasma membrane protein and involved in cellular Ca2+ homeostasis especially in cells that require influx of Ca2+ for their physiological activities such as cardiac myocytes and neurons. NCX contains 9 t ...
... Cytosolic Ca2+ plays an important role in intracellular signaling. Na+/Ca2+ exchanger (NCX) is a plasma membrane protein and involved in cellular Ca2+ homeostasis especially in cells that require influx of Ca2+ for their physiological activities such as cardiac myocytes and neurons. NCX contains 9 t ...
Microbial enzyme secretion
... ª 2017 The Authors. Microbial Biotechnology published by John Wiley & Sons Ltd and Society for Applied Microbiology. This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work ...
... ª 2017 The Authors. Microbial Biotechnology published by John Wiley & Sons Ltd and Society for Applied Microbiology. This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work ...
Keep Your Fingers Off My DNA: Protein–Protein
... Gata1 (FOG1) and its binding partners, the globin transcription factor GATA1 and Transforming Acidic Coiledcoil 3 (TACC3). FOG1 contains a total of nine ZFs (Table 2); however, only four are of the classic C2H2 type. The remaining fingers substitute a conserved cysteine for the final histidine [36]. ...
... Gata1 (FOG1) and its binding partners, the globin transcription factor GATA1 and Transforming Acidic Coiledcoil 3 (TACC3). FOG1 contains a total of nine ZFs (Table 2); however, only four are of the classic C2H2 type. The remaining fingers substitute a conserved cysteine for the final histidine [36]. ...
Not Every Disulfide Lasts Forever: Disulfide Bond
... When ROS concentrations rise above a certain level, these scavengers are no longer sufficient to prevent oxidative damage, putting the cell into a state that is defined as oxidative stress (59). This appears to be a major factor in aging (16), and has been implicated in numerous diseases such as Alz ...
... When ROS concentrations rise above a certain level, these scavengers are no longer sufficient to prevent oxidative damage, putting the cell into a state that is defined as oxidative stress (59). This appears to be a major factor in aging (16), and has been implicated in numerous diseases such as Alz ...
The Localization and Secretion of a Proteolytic
... rather lower yields on casein hydrolysate. Proteolytic enzymes were found in the culture filtrate when the organism was growing on casein but not when casein hydrolysate was present in the medium. The enzymes appeared first at the beginning of the exponential phase of growth and steadily increased u ...
... rather lower yields on casein hydrolysate. Proteolytic enzymes were found in the culture filtrate when the organism was growing on casein but not when casein hydrolysate was present in the medium. The enzymes appeared first at the beginning of the exponential phase of growth and steadily increased u ...
Hsp70 and Hsp90 of E. coli Directly Interact for Collaboration in
... [21], lose adaptive immunity conferred by the CRISPR system [22] and show a subtle defect in motility [23]. Additionally, when Hsp90Ec is overexpressed in E. coli, cells filament and become sensitive to SDS [5]. Both eukaryotic Hsp90 and Hsp90Ec have been shown to remodel proteins in vitro. For exam ...
... [21], lose adaptive immunity conferred by the CRISPR system [22] and show a subtle defect in motility [23]. Additionally, when Hsp90Ec is overexpressed in E. coli, cells filament and become sensitive to SDS [5]. Both eukaryotic Hsp90 and Hsp90Ec have been shown to remodel proteins in vitro. For exam ...
PDF - SAGE Journals
... requires only CO2 as a carbon source for synthesizing all necessary biomolecules. Changes in the environmental availability of these molecules can be expected to activate regulatory mechanisms manifested as the up and down regulation of specific genes and the concomitant increase and decrease in abu ...
... requires only CO2 as a carbon source for synthesizing all necessary biomolecules. Changes in the environmental availability of these molecules can be expected to activate regulatory mechanisms manifested as the up and down regulation of specific genes and the concomitant increase and decrease in abu ...
Disulfide formation in plant storage vacuoles permits assembly
... compost and vermiculite under greenhouse conditions at 15°C with a 16 hour light / 8 hour dark cycle. Prior to planting, seeds were imbibed in running water overnight. The development of Ricinus communis seeds is divided into seven stages (A to G) based on size, testa formation and state of hydratio ...
... compost and vermiculite under greenhouse conditions at 15°C with a 16 hour light / 8 hour dark cycle. Prior to planting, seeds were imbibed in running water overnight. The development of Ricinus communis seeds is divided into seven stages (A to G) based on size, testa formation and state of hydratio ...
Inhibition Systems Mechanisms and Biological Roles of
... distinct CdiA-CT sequence types have been identified, and all CDI toxins characterized thus far display RNase, DNase, or pore-forming activities. CDI systems also encode CdiI immunity proteins, which specifically bind and inactivate cognate CdiA-CT toxins to prevent autoinhibition. CDI activity appe ...
... distinct CdiA-CT sequence types have been identified, and all CDI toxins characterized thus far display RNase, DNase, or pore-forming activities. CDI systems also encode CdiI immunity proteins, which specifically bind and inactivate cognate CdiA-CT toxins to prevent autoinhibition. CDI activity appe ...
Bacteriophage EMS9: Preliminary Genomic Description
... number of amino acids predicted to comprise the protein encoded by each numbered ORF is listed first, followed by the position (in base pairs) where the ORF occurs, the DNA strand on which the information is encoded and finally, the nature of the predicted protein is listed. Certain of the ORFs list ...
... number of amino acids predicted to comprise the protein encoded by each numbered ORF is listed first, followed by the position (in base pairs) where the ORF occurs, the DNA strand on which the information is encoded and finally, the nature of the predicted protein is listed. Certain of the ORFs list ...
Engineering key components in a synthetic eukaryotic
... assays to be developed for plant HKs and AHPs in bacteria and yeast (Inoue et al, 2001; Yamada et al, 2001; Reiser et al, 2003). Conservation and modularity can be further seen in an alignment of the receiver domain from the bacterial RRs, PhoB and OmpR, with the receiver domains of multiple plant H ...
... assays to be developed for plant HKs and AHPs in bacteria and yeast (Inoue et al, 2001; Yamada et al, 2001; Reiser et al, 2003). Conservation and modularity can be further seen in an alignment of the receiver domain from the bacterial RRs, PhoB and OmpR, with the receiver domains of multiple plant H ...
The Proteomic Code: a molecular recognition code for proteins
... George Gamow was a theoretical physicist and cosmologist and spent only a few years in Cambridge, UK, but he was there when the structure of DNA was discovered in 1953. He developed the first genetic code, which was not only an elegant solution for the problem of information transfer from DNA to pro ...
... George Gamow was a theoretical physicist and cosmologist and spent only a few years in Cambridge, UK, but he was there when the structure of DNA was discovered in 1953. He developed the first genetic code, which was not only an elegant solution for the problem of information transfer from DNA to pro ...
Encontro de Jovens Investigadores de Biologia Computacional
... chemotherapy failures worldwide. Although P-glycoprotein (P-gp) is one of the main contributors for MDR, the biophysical aspects of drug efflux by P-gp still remain largely unknown. Following our characterization of three drug-binding sites within P-gp\'s internal drug-binding pocket (DBP), a series ...
... chemotherapy failures worldwide. Although P-glycoprotein (P-gp) is one of the main contributors for MDR, the biophysical aspects of drug efflux by P-gp still remain largely unknown. Following our characterization of three drug-binding sites within P-gp\'s internal drug-binding pocket (DBP), a series ...
How do bacterial cells ensure that metalloproteins get the correct
... iron–sulphur clusters, and in a small subset of proteins (including ribonucleotide reductase and soluble methane monooxygenase) that directly bind one or two atoms of iron without any associated tetrapyrrole or inorganic sulphide6. In contrast to iron, zinc has a single oxidation state in solution a ...
... iron–sulphur clusters, and in a small subset of proteins (including ribonucleotide reductase and soluble methane monooxygenase) that directly bind one or two atoms of iron without any associated tetrapyrrole or inorganic sulphide6. In contrast to iron, zinc has a single oxidation state in solution a ...
effects of insulin and anchorage on hepatocytic protein metabolism
... Cycloheximide, a strong inhibitor of hepatocytic protein synthesis (Seglen, 1977), was previously found not to affect protein degradation in i-h experiments with isolated hepatocytes (Seglen et al. 1979); however, in long-term experiments with perfused livers (Woodside, 1976; Khairallah & Mortimore, ...
... Cycloheximide, a strong inhibitor of hepatocytic protein synthesis (Seglen, 1977), was previously found not to affect protein degradation in i-h experiments with isolated hepatocytes (Seglen et al. 1979); however, in long-term experiments with perfused livers (Woodside, 1976; Khairallah & Mortimore, ...
Signaling from synapse to nucleus: the logic behind the mechanisms Karl Deisseroth
... and to understand why DREAM is present outside the nucleus as well as within it. Are there particular advantages for the signaling mechanisms at either one or the other of these extremes? On the one hand, the nuclear Ca2þ mechanism that is exemplified by DREAM is straightforward, fast and simple, bu ...
... and to understand why DREAM is present outside the nucleus as well as within it. Are there particular advantages for the signaling mechanisms at either one or the other of these extremes? On the one hand, the nuclear Ca2þ mechanism that is exemplified by DREAM is straightforward, fast and simple, bu ...
The water of life: osmoregulation in Saccharomyces cerevisiae
... Interactions between the target of rapamycin (TOR) and the CWI pathways during nutrient stress, and effects on osmotolerance ...
... Interactions between the target of rapamycin (TOR) and the CWI pathways during nutrient stress, and effects on osmotolerance ...
Protein phosphorylation
Protein phosphorylation is a post-translational modification of proteins in which an amino acid residue is phosphorylated by a protein kinase by the addition of a covalently bound phosphate group. Phosphorylation alters the structural conformation of a protein, causing it to become activated, deactivated, or modifying its function. The reverse reaction of phosphorylation is called dephosphorylation, and is catalyzed by protein phosphatases. Protein kinases and phosphatases work independently and in a balance to regulate the function of proteins. The amino acids most commonly phosphorylated are serine, threonine, and tyrosine in eukaryotes, and histidine in prokaryotes, which play important and well-characterized roles in signaling pathways and metabolism. However, many other amino acids can also be phosphorylated, including arginine, lysine, and cysteine. Protein phosphorylation was first reported in 1906 by Phoebus Levene at the Rockefeller Institute for Medical Research with the discovery of phosphorylated vitellin. However, it was nearly 50 years until the enzymatic phosphorylation of proteins by protein kinases was discovered.