Sequential Expression of Vascular Endothelial Growth Factor, Flt
... First, we found that VEGF and it receptors (Flt-1 and KDR/Flk-1) are expressed in the same regenerating muscle fibers. Second, we found that their gene expression appears to be coordinated with muscle regeneration. The present study further shows that regenerating muscle fibers potentially express V ...
... First, we found that VEGF and it receptors (Flt-1 and KDR/Flk-1) are expressed in the same regenerating muscle fibers. Second, we found that their gene expression appears to be coordinated with muscle regeneration. The present study further shows that regenerating muscle fibers potentially express V ...
Procedures Manual - Eye Bank Association of America
... Manual served as a detailed source of information and training manual for new technicians. This Procedures Manual is not intended to replace the Technician's Manual or to serve as a training manual for new eye bank technicians. The purpose of this manual is to operationalize the EBAA Medical Standar ...
... Manual served as a detailed source of information and training manual for new technicians. This Procedures Manual is not intended to replace the Technician's Manual or to serve as a training manual for new eye bank technicians. The purpose of this manual is to operationalize the EBAA Medical Standar ...
Facial Augmentation With Structural Fat Grafting
... through large incisions. Even though Conrad Miller reported good results with the injected fat, the technique he described never became popular. Soon after the early descriptions of suction curettage of fat for body contouring [7,8], Chajchir and Benzaquen [9] described injecting suctioned fat into ...
... through large incisions. Even though Conrad Miller reported good results with the injected fat, the technique he described never became popular. Soon after the early descriptions of suction curettage of fat for body contouring [7,8], Chajchir and Benzaquen [9] described injecting suctioned fat into ...
Determination of the Correlation between Conductance and Architecture of Disordered ß-amyloid Channels
... lipids, results in the reduction of the conductance dynamic range for Scenario II (Table 3) (0.6810.36 nS) as compared to Scenario I (Table 2) (0.31-15.90 nS). The charged lipids are negatively charged, which draws cations towards the surface. When the pore is small, this increases the proximity of ...
... lipids, results in the reduction of the conductance dynamic range for Scenario II (Table 3) (0.6810.36 nS) as compared to Scenario I (Table 2) (0.31-15.90 nS). The charged lipids are negatively charged, which draws cations towards the surface. When the pore is small, this increases the proximity of ...
PDF - The Gould Lab
... Experiment 1: delay conditioning with a very long delay. Rats were injected intraperitoneally with bromodeoxyuridine (BrdU; 200 mg/kg), a marker of dividing cells. One week later, rats underwent 800 trials of eyeblink conditioning (200 trials/d for 4 d) using a trace (n ⫽ 8), delay (n ⫽ 7), or very ...
... Experiment 1: delay conditioning with a very long delay. Rats were injected intraperitoneally with bromodeoxyuridine (BrdU; 200 mg/kg), a marker of dividing cells. One week later, rats underwent 800 trials of eyeblink conditioning (200 trials/d for 4 d) using a trace (n ⫽ 8), delay (n ⫽ 7), or very ...
Nociceptor and Hair Cell Transducer Properties of TRPA1, a
... (Corey et al., 2004). We wondered whether TRPA1 would be part of transduction channels in both mammalian nociceptors and hair cells. Here, we present conclusive evidence that TRPA1, in addition to being expressed in nociceptor neurons, is present in hair cell stereocilia. We also demonstrate that he ...
... (Corey et al., 2004). We wondered whether TRPA1 would be part of transduction channels in both mammalian nociceptors and hair cells. Here, we present conclusive evidence that TRPA1, in addition to being expressed in nociceptor neurons, is present in hair cell stereocilia. We also demonstrate that he ...
OPTIMISATION OF CHO CELL GROWTH AND
... was not possible in its absence. Pluronic F68, alone or in combination with a lipid mixture or linoleic acid, was able to restore cell growth in low BSA (1 mg/ml) cultures. However, JFN-y production was significantly reduced and the extent of IFN-y glycosylation also changed. These effects were rela ...
... was not possible in its absence. Pluronic F68, alone or in combination with a lipid mixture or linoleic acid, was able to restore cell growth in low BSA (1 mg/ml) cultures. However, JFN-y production was significantly reduced and the extent of IFN-y glycosylation also changed. These effects were rela ...
regulation of the cytoskeleton and cell adhesion by
... Guanine Nucleotide Exchange Factors There are guanine nucleotide exchange factors (GEFs), also known as GDP dissociation stimulators (GDSs), that facilitate the release of GDP from the Rho family GTPases, thereby promoting the binding of GTP (33) because the cytosolic concentration of GTP is more th ...
... Guanine Nucleotide Exchange Factors There are guanine nucleotide exchange factors (GEFs), also known as GDP dissociation stimulators (GDSs), that facilitate the release of GDP from the Rho family GTPases, thereby promoting the binding of GTP (33) because the cytosolic concentration of GTP is more th ...
Chara Myosin and the Energy of Cytoplasmic Streaming
... cannot stop all myosin molecules at once. If one myosin molecule stops moving due to the glutaraldehyde fixation, it will disturb other myosin molecules following the same actin track. This small stack will affect the movement of myosin molecules on the next actin filament in the same cable. An intr ...
... cannot stop all myosin molecules at once. If one myosin molecule stops moving due to the glutaraldehyde fixation, it will disturb other myosin molecules following the same actin track. This small stack will affect the movement of myosin molecules on the next actin filament in the same cable. An intr ...
Foxp3-positive macrophages display immunosuppressive properties
... lymph nodes, bone marrow, thymus, liver, and other tissues of naive animals. To characterize this subpopulation of macrophages, we devised a strategy to purify CD11b+F4/80+Foxp3+ macrophages using Foxp3-GFP mice. Analysis of CD11b+F4/80+Foxp3+ macrophage function indicated that these cells inhibited ...
... lymph nodes, bone marrow, thymus, liver, and other tissues of naive animals. To characterize this subpopulation of macrophages, we devised a strategy to purify CD11b+F4/80+Foxp3+ macrophages using Foxp3-GFP mice. Analysis of CD11b+F4/80+Foxp3+ macrophage function indicated that these cells inhibited ...
Distinct and collaborative roles of Drosophila EXT family proteins in
... together, these results suggest that both botv and sotv are segment-polarity genes, and are likely to be involved in Hh and/or Wg signalling. botv and sotv encode the Drosophila homologues of mammalian EXTL3 and EXT2, respectively, and are required for HS GAG biosynthesis botv and sotv were subseque ...
... together, these results suggest that both botv and sotv are segment-polarity genes, and are likely to be involved in Hh and/or Wg signalling. botv and sotv encode the Drosophila homologues of mammalian EXTL3 and EXT2, respectively, and are required for HS GAG biosynthesis botv and sotv were subseque ...
Original Article Intracellular zinc distribution in mitochondria, ER and
... In this study, we demonstrate that zinc is present within mitochondria, ER and Golgi apparatuses (Figures 2-4), providing the direct evidence that these organelles serve as candidates of intracellular zinc storages. We also show that zinc is not detectable in the nucleus (Figure 5). It is due to tha ...
... In this study, we demonstrate that zinc is present within mitochondria, ER and Golgi apparatuses (Figures 2-4), providing the direct evidence that these organelles serve as candidates of intracellular zinc storages. We also show that zinc is not detectable in the nucleus (Figure 5). It is due to tha ...
Biofilms
... The biofilm matrix Most of the biomass of the biofilm comprises hydrated EPS rather than microbial cells17. The self-organization of EPS molecules in the matrix is based on inter molecular interactions between EPS components, which also determine the mechanical properties of the matrix, and the ph ...
... The biofilm matrix Most of the biomass of the biofilm comprises hydrated EPS rather than microbial cells17. The self-organization of EPS molecules in the matrix is based on inter molecular interactions between EPS components, which also determine the mechanical properties of the matrix, and the ph ...
COWDRIA RUMINANTIUMIN PROTECTIVE ROLE OF LYT -2+ T CELLS J .
... Kiimm or the Welgevonden stocks of Cowdria ruminantium and their inability to mount an immune response, suggest that immunity in heartwater is cell-mediated. The adoptive transfer of immunity with the spleen cells of mice immune to the Welgevonden stock is supportive evidence. Immune spleen cells de ...
... Kiimm or the Welgevonden stocks of Cowdria ruminantium and their inability to mount an immune response, suggest that immunity in heartwater is cell-mediated. The adoptive transfer of immunity with the spleen cells of mice immune to the Welgevonden stock is supportive evidence. Immune spleen cells de ...
Wounds and Scars - Tulane University
... numbers are maintained by mitosis. Fixed basal cells away from the wound edge begin mitosis to replace the migrating cells, and as resurfacing of the wound proceeds, the cells that have migrated in turn start to divide and multiply. Increasing numbers of cells thicken the new epithelial layer. Upon ...
... numbers are maintained by mitosis. Fixed basal cells away from the wound edge begin mitosis to replace the migrating cells, and as resurfacing of the wound proceeds, the cells that have migrated in turn start to divide and multiply. Increasing numbers of cells thicken the new epithelial layer. Upon ...
measuring force in the developing zebrafish embryo using
... removed from its cadherin complex [10]. Vinculin itself has also been shown to hold tension and recruit adherens junctions in a force dependent manner [1]. Cellular remodeling in response to force has also been observed in the whole embryo. During zebrafish epiboly, cell divisions in the outer envel ...
... removed from its cadherin complex [10]. Vinculin itself has also been shown to hold tension and recruit adherens junctions in a force dependent manner [1]. Cellular remodeling in response to force has also been observed in the whole embryo. During zebrafish epiboly, cell divisions in the outer envel ...
In tobacco leaf epidermal cells, the integrity of protein export from
... 96 Giovanni Stefano et al. COPI-coated vesicles have been observed predominantly at cis and medial Golgi cisternae (Pimpl et al., 2000). However, a full characterization of the dynamics of COPI at the Golgi apparatus in living plant cells has yet to emerge. It has been shown that Arf1 localizes at ...
... 96 Giovanni Stefano et al. COPI-coated vesicles have been observed predominantly at cis and medial Golgi cisternae (Pimpl et al., 2000). However, a full characterization of the dynamics of COPI at the Golgi apparatus in living plant cells has yet to emerge. It has been shown that Arf1 localizes at ...
In tobacco leaf epidermal cells, the integrity of protein export from
... 2 Giovanni Stefano et al. COPI-coated vesicles have been observed predominantly at cis and medial Golgi cisternae (Pimpl et al., 2000). However, a full characterization of the dynamics of COPI at the Golgi apparatus in living plant cells has yet to emerge. It has been shown that Arf1 localizes at t ...
... 2 Giovanni Stefano et al. COPI-coated vesicles have been observed predominantly at cis and medial Golgi cisternae (Pimpl et al., 2000). However, a full characterization of the dynamics of COPI at the Golgi apparatus in living plant cells has yet to emerge. It has been shown that Arf1 localizes at t ...
Endothelial Barrier Function Adenosine
... (adenosine) could transcriptionally regulate surface enzyme (CD73). To date, no direct evidence exists for this pathway. In these studies, we explored the regulation of endothelial CD73 bioactivity; surface protein expression; and transcriptional activation by adenosine, adenosine analogues, and oth ...
... (adenosine) could transcriptionally regulate surface enzyme (CD73). To date, no direct evidence exists for this pathway. In these studies, we explored the regulation of endothelial CD73 bioactivity; surface protein expression; and transcriptional activation by adenosine, adenosine analogues, and oth ...
Suppressor analysis of the protein kinase Elm1p, an enzyme
... quality of nutrients available, such that poor carbon or nitrogen sources result in smaller size requirements before commitment to the next round of cell division (60, 90). Thus nutrient source quality also regulates cell size in S. cerevisiae. Under specific conditions of nitrogen starvation and in ...
... quality of nutrients available, such that poor carbon or nitrogen sources result in smaller size requirements before commitment to the next round of cell division (60, 90). Thus nutrient source quality also regulates cell size in S. cerevisiae. Under specific conditions of nitrogen starvation and in ...
BAFF, APRIL and their receptors: Structure, function - Serval
... subject to rarer intergenic splicing events removing the transmembrane and intracellular domains to yield soluble forms of TACI. However, TACI being a Type III protein, these proteins are not predicted to be secreted (Fig. 2A). In mouse TACI, the initiating methionine and CRD1 are in the same exon. ...
... subject to rarer intergenic splicing events removing the transmembrane and intracellular domains to yield soluble forms of TACI. However, TACI being a Type III protein, these proteins are not predicted to be secreted (Fig. 2A). In mouse TACI, the initiating methionine and CRD1 are in the same exon. ...
Tcf7l1 protects the anterior neural fold from adopting the neural crest
... requirement for Wnt/β-catenin signaling from the earliest steps of NC development, at first during gastrulation when Wnt/β-catenin signaling drives the expression of the NC specifier genes Pax3 (Bang et al., 1999; Garnett et al., 2012; Taneyhill and BronnerFraser, 2005; Zhao et al., 2014), Foxd3 (Ja ...
... requirement for Wnt/β-catenin signaling from the earliest steps of NC development, at first during gastrulation when Wnt/β-catenin signaling drives the expression of the NC specifier genes Pax3 (Bang et al., 1999; Garnett et al., 2012; Taneyhill and BronnerFraser, 2005; Zhao et al., 2014), Foxd3 (Ja ...
A Phosphorylation State-specific Antibody Recognizes Hsp27, a
... K/E/Q/A)S*XXXX, where X represents a position in the peptide synthesis where a mixture of all 20 amino acid (excluding C and W) were used, and where S* represent phosphoserine. The peptide was conjugated to keyhole limpet hemocyanin and used to immunize rabbits. Phosphopeptide-reactive rabbit antise ...
... K/E/Q/A)S*XXXX, where X represents a position in the peptide synthesis where a mixture of all 20 amino acid (excluding C and W) were used, and where S* represent phosphoserine. The peptide was conjugated to keyhole limpet hemocyanin and used to immunize rabbits. Phosphopeptide-reactive rabbit antise ...
Spatiotemporal Patterning of Reactive Oxygen Production and Ca2+
... cytosolic Ca2 increase. Thus, the spatiotemporal pattern of the Ca 2 wave is determined by peripheral ROS production. The second, later ROS component localizes to the mitochondria and is a direct consequence of the Ca 2 wave. The first component, but not the second, is required for short-term ada ...
... cytosolic Ca2 increase. Thus, the spatiotemporal pattern of the Ca 2 wave is determined by peripheral ROS production. The second, later ROS component localizes to the mitochondria and is a direct consequence of the Ca 2 wave. The first component, but not the second, is required for short-term ada ...
NYS Lab: Diffusion Through a Membrane
... • exposure to a strongly hypotonic solution resulted in the cell's ability to quickly adjust to ...
... • exposure to a strongly hypotonic solution resulted in the cell's ability to quickly adjust to ...
Tissue engineering
Tissue engineering is the use of a combination of cells, engineering and materials methods, and suitable biochemical and physicochemical factors to improve or replace biological functions. While it was once categorized as a sub-field of biomaterials, having grown in scope and importance it can be considered as a field in its own right.While most definitions of tissue engineering cover a broad range of applications, in practice the term is closely associated with applications that repair or replace portions of or whole tissues (i.e., bone, cartilage, blood vessels, bladder, skin, muscle etc.). Often, the tissues involved require certain mechanical and structural properties for proper functioning. The term has also been applied to efforts to perform specific biochemical functions using cells within an artificially-created support system (e.g. an artificial pancreas, or a bio artificial liver). The term regenerative medicine is often used synonymously with tissue engineering, although those involved in regenerative medicine place more emphasis on the use of stem cells or progenitor cells to produce tissues.