The Iron-Sulfur Cluster Proteins Isa1 and Isa2 Are Required for the
... ISA1 was exchanged for the galactose-inducible GAL1-10 promoter by PCRmediated gene replacement. The functionality of various BIO2 constructs was tested below (see Fig. 3A) using bio2⌬::kanMX4 cells (BY4742 YGR286C⌬::kanMX4) (EUROSCARF, Frankfurt/Main, Germany). Cells were grown in rich (YP [1% yeas ...
... ISA1 was exchanged for the galactose-inducible GAL1-10 promoter by PCRmediated gene replacement. The functionality of various BIO2 constructs was tested below (see Fig. 3A) using bio2⌬::kanMX4 cells (BY4742 YGR286C⌬::kanMX4) (EUROSCARF, Frankfurt/Main, Germany). Cells were grown in rich (YP [1% yeas ...
Mechanism of isoniazid uptake in Mycobacterium
... proposed that [“C]INH enters cells by passive diffusion through the bacterial envelope, and that its oxidation by KatG then leads to the accumulation of radioactive derivatives inside the cells. A consequence of this model is that the initial transport velocity of INH is independent of KatG, but lab ...
... proposed that [“C]INH enters cells by passive diffusion through the bacterial envelope, and that its oxidation by KatG then leads to the accumulation of radioactive derivatives inside the cells. A consequence of this model is that the initial transport velocity of INH is independent of KatG, but lab ...
Tissue interactions in the developing chick diencephalon
... explanted from the embryo and kept in a neutral environment, such as serum-free tissue culture. A tissue has become determined if it will differentiate according to its fate even after it has been grafted to an ectopic location in the embryo where other patterning influences may per- ...
... explanted from the embryo and kept in a neutral environment, such as serum-free tissue culture. A tissue has become determined if it will differentiate according to its fate even after it has been grafted to an ectopic location in the embryo where other patterning influences may per- ...
Graded potential of neural crest to form cornea, sensory neurons
... neural crest has a broader (or at least different) developmental potential than that of trunk neural crest. However, under appropriate culture conditions, trunk neural crest cells have been shown to acquire some properties of chondrocytes (McGonnell and Graham, 2002). The fact that trunk neural cres ...
... neural crest has a broader (or at least different) developmental potential than that of trunk neural crest. However, under appropriate culture conditions, trunk neural crest cells have been shown to acquire some properties of chondrocytes (McGonnell and Graham, 2002). The fact that trunk neural cres ...
Graded potential of neural crest to form cornea, sensory neurons
... neural crest has a broader (or at least different) developmental potential than that of trunk neural crest. However, under appropriate culture conditions, trunk neural crest cells have been shown to acquire some properties of chondrocytes (McGonnell and Graham, 2002). The fact that trunk neural cres ...
... neural crest has a broader (or at least different) developmental potential than that of trunk neural crest. However, under appropriate culture conditions, trunk neural crest cells have been shown to acquire some properties of chondrocytes (McGonnell and Graham, 2002). The fact that trunk neural cres ...
A novel genetic strategy reveals unexpected roles of the Swi
... CD4 expression on these cells (8, 9). Although the BAF complex can remodel chromatin, it might have functions in gene regulation beyond classical ATP-dependent remodeling, as suggested by the following data. Specifically, the ATPase activity of Brg and, therefore, chromatin remodeling by the BAF com ...
... CD4 expression on these cells (8, 9). Although the BAF complex can remodel chromatin, it might have functions in gene regulation beyond classical ATP-dependent remodeling, as suggested by the following data. Specifically, the ATPase activity of Brg and, therefore, chromatin remodeling by the BAF com ...
Treating Skin Traumas Using PolyMem
... disruptive contact with a foreign entity, skin trauma ranges from simple lacerations to burns and twisting tears. The common factor among all skin traumas is the exposure of sub-dermal structures and organs for which the skin is the natural defense against infection and the environment. The skin’s s ...
... disruptive contact with a foreign entity, skin trauma ranges from simple lacerations to burns and twisting tears. The common factor among all skin traumas is the exposure of sub-dermal structures and organs for which the skin is the natural defense against infection and the environment. The skin’s s ...
Xenopus ADAM13 and ADAM19 are Important for Proper
... the dorsal mesoderm; mediolateral cell intercalation and convergence and extension. During gastrulation, mesoderm cells send protrusions to gain traction on neighboring cells and the surrounding extracellular matrix; a process called mediolateral cell intercalation. Mesoderm cells use this type of c ...
... the dorsal mesoderm; mediolateral cell intercalation and convergence and extension. During gastrulation, mesoderm cells send protrusions to gain traction on neighboring cells and the surrounding extracellular matrix; a process called mediolateral cell intercalation. Mesoderm cells use this type of c ...
Cotto J. Fox S. Morimoto R . HSF1 granules
... Similar results of co-localization were observed using HSF1GFP (Fig. 4). Whereas GFP alone is distributed in a diffuse pattern throughout the cell under control (Fig. 4A) and heat shocked conditions (Fig. 4B), mHSF1-GFP is primarily nuclear in control cells (Fig. 4C) and upon heat shock relocalizes ...
... Similar results of co-localization were observed using HSF1GFP (Fig. 4). Whereas GFP alone is distributed in a diffuse pattern throughout the cell under control (Fig. 4A) and heat shocked conditions (Fig. 4B), mHSF1-GFP is primarily nuclear in control cells (Fig. 4C) and upon heat shock relocalizes ...
The Arabidopsis homeotic genes APETALA3 and PISTILLATA are
... generated by crossing plants containing the p35S-AP3 construct (Jack et al., 1994) to p35S-PI plants. Flowers of these plants (p35S-PI p35S-AP3) have two outer whorls of petals and inner whorls of stamens (Figs 1C, 2E, compare with wild type, Figs 1A, 2A). These first and fourth whorl transformation ...
... generated by crossing plants containing the p35S-AP3 construct (Jack et al., 1994) to p35S-PI plants. Flowers of these plants (p35S-PI p35S-AP3) have two outer whorls of petals and inner whorls of stamens (Figs 1C, 2E, compare with wild type, Figs 1A, 2A). These first and fourth whorl transformation ...
Plasma Membrane Ghosts Form Differently When Produced from
... controlled elongation to isodiametric expansion by removing the controls on cellulose deposition. Cortical microtubules are retained after cell elongation ceases, although the pattern of the microtubules changes away from transverse to longitudinal or oblique depending on the system, and while ho lo ...
... controlled elongation to isodiametric expansion by removing the controls on cellulose deposition. Cortical microtubules are retained after cell elongation ceases, although the pattern of the microtubules changes away from transverse to longitudinal or oblique depending on the system, and while ho lo ...
Auxin and self-organization at the shoot apical
... animals lies in the regulation of their organogenetic capacity over time. Unlike most animals, in which organogenesis occurs during embryonic development, plants can form new organs throughout their life cycle. Post-embryonic development occurs thanks to specialized tissues containing stem-cell nich ...
... animals lies in the regulation of their organogenetic capacity over time. Unlike most animals, in which organogenesis occurs during embryonic development, plants can form new organs throughout their life cycle. Post-embryonic development occurs thanks to specialized tissues containing stem-cell nich ...
The Qualitative Effects of Resveratrol ... Administration on the Gluteus Complex ...
... with a disrupted plasma membrane; however, with defective protein function these vesicles accumulate beneath the disruption site but are unable to fuse with it and reseal the membrane, thus rendering the membrane repair mechanism defective. The SJL/J mouse model presents with characteristics much li ...
... with a disrupted plasma membrane; however, with defective protein function these vesicles accumulate beneath the disruption site but are unable to fuse with it and reseal the membrane, thus rendering the membrane repair mechanism defective. The SJL/J mouse model presents with characteristics much li ...
The Crucial Role of Biofilms in Cryptococcus neoformans Survival
... phagosomes, causing increases in pH and subsequently lowering the microbial proliferation rate [44]. This finding not only stresses the importance of acidic environments for fungal growth but also brings forth a model of cryptococcal survival in the CNS. Although phagosomal conditions are ideal for ...
... phagosomes, causing increases in pH and subsequently lowering the microbial proliferation rate [44]. This finding not only stresses the importance of acidic environments for fungal growth but also brings forth a model of cryptococcal survival in the CNS. Although phagosomal conditions are ideal for ...
- Wiley Online Library
... as well as in Movies S1–S3 (Movie S3 shows both LeEix2 and FYVE localization). Nicotiana benthamiana cells that transiently express tagged FYVE exhibit many endosomes that vary in their size, shape and intensity of localized FYVE. The effect of EIX on endosomal dynamics is perhaps not surprising, as ...
... as well as in Movies S1–S3 (Movie S3 shows both LeEix2 and FYVE localization). Nicotiana benthamiana cells that transiently express tagged FYVE exhibit many endosomes that vary in their size, shape and intensity of localized FYVE. The effect of EIX on endosomal dynamics is perhaps not surprising, as ...
BoneScalpel User Manual
... accuracy. To assess the electromagnetic environment due to fixed RF transmitters, an electromagnetic site survey should be considered. If the measured field strength in the location in which the BONESCALPEL SYSTEM is used exceeds the applicable RF compliance level above, the BONESCALPEL SYSTEM shoul ...
... accuracy. To assess the electromagnetic environment due to fixed RF transmitters, an electromagnetic site survey should be considered. If the measured field strength in the location in which the BONESCALPEL SYSTEM is used exceeds the applicable RF compliance level above, the BONESCALPEL SYSTEM shoul ...
Uncoupling effect of polyunsaturated fatty acid deficiency in isolated
... Increased metabolic rate accompanied by reduced growth has been reported in polyunsaturated fatty acid (PUFA)-deficient rats [1–3]. The mechanism of this effect is still a matter of debate. Several authors [4,5] have suggested that it is due to a decrease in the efficiency of oxidative phosphorylati ...
... Increased metabolic rate accompanied by reduced growth has been reported in polyunsaturated fatty acid (PUFA)-deficient rats [1–3]. The mechanism of this effect is still a matter of debate. Several authors [4,5] have suggested that it is due to a decrease in the efficiency of oxidative phosphorylati ...
Dazl regulates mouse embryonic germ cell development
... In the mouse, germ cells can undergo differentiation to become either oocytes or spermatozoa in response to sex of their gonadal environment. The nature of the germ cell-intrinsic aspects of this signaling have not been well studied. The earliest known sex-specific difference in germ cells is the in ...
... In the mouse, germ cells can undergo differentiation to become either oocytes or spermatozoa in response to sex of their gonadal environment. The nature of the germ cell-intrinsic aspects of this signaling have not been well studied. The earliest known sex-specific difference in germ cells is the in ...
Soluble factors from human endometrium promote angiogenesis
... Human umbilical vein EC(s) (HUVEC) were isolated by collagenase digestion as described (Jaffe et al., 1973) and cultured to passage 5 in LVEC medium. Once at passage 5, HUVEC were plated into 24-well plates at 70% confluence and supernatants from endometrial cultures added (20% V/V) for 24 h. Viable ...
... Human umbilical vein EC(s) (HUVEC) were isolated by collagenase digestion as described (Jaffe et al., 1973) and cultured to passage 5 in LVEC medium. Once at passage 5, HUVEC were plated into 24-well plates at 70% confluence and supernatants from endometrial cultures added (20% V/V) for 24 h. Viable ...
Levels of Ycg1 Limit Condensin Function during the Cell Cycle
... Ycg1 protein and transcript levels are cell cycle-regulated Although the budding yeast condensin complex associates with chromatin throughout the cell cycle [6,7,15,17], its activity increases substantially during mitosis. Previous reports have shown that this change in activity is due in part to in ...
... Ycg1 protein and transcript levels are cell cycle-regulated Although the budding yeast condensin complex associates with chromatin throughout the cell cycle [6,7,15,17], its activity increases substantially during mitosis. Previous reports have shown that this change in activity is due in part to in ...
Enhanced intracellular Ca concentrations in and after addition
... elicitation on fungal morphology and penicillin G production has also been studied: Penicillium chrysogenum supplemented with oligosaccharide elicitors had higher hyphal tip numbers compared to control cultures. Enhancement of penicillin G in elicited cultures may be related to the morphological cha ...
... elicitation on fungal morphology and penicillin G production has also been studied: Penicillium chrysogenum supplemented with oligosaccharide elicitors had higher hyphal tip numbers compared to control cultures. Enhancement of penicillin G in elicited cultures may be related to the morphological cha ...
Cell-cycle-regulated expression of STIL controls centriole number in
... structural homologs outside of nematodes. Thus, it is of considerable interest that the Drosophila protein Ana2 has recently been identified as a potential functional ortholog of SAS-5 (Stevens et al., 2010). Furthermore, database searches led to the suggestion that STIL (SCL/TAL1 interrupting locus ...
... structural homologs outside of nematodes. Thus, it is of considerable interest that the Drosophila protein Ana2 has recently been identified as a potential functional ortholog of SAS-5 (Stevens et al., 2010). Furthermore, database searches led to the suggestion that STIL (SCL/TAL1 interrupting locus ...
Host cell-derived lactate functions as an effector molecule in
... We next investigated the time required for the medium to accumulate a sufficient amount of the microcolony dispersal factor(s). While CM collected between 5 min and 5 hours all accelerated the dispersal, the effect increased with time, with the fastest dispersal in CM collected after 5 h (Fig 2D). T ...
... We next investigated the time required for the medium to accumulate a sufficient amount of the microcolony dispersal factor(s). While CM collected between 5 min and 5 hours all accelerated the dispersal, the effect increased with time, with the fastest dispersal in CM collected after 5 h (Fig 2D). T ...
Salivary gland development in Drosophila
... at much higher levels in the salivary gland secretory primordia than in other embryonic tissues. This high level of transcription continues throughout embryogenesis (E. Abrams and D.J.A., unpublished data). By late embryogenesis, active secretion is evident by light and transmission electron microsc ...
... at much higher levels in the salivary gland secretory primordia than in other embryonic tissues. This high level of transcription continues throughout embryogenesis (E. Abrams and D.J.A., unpublished data). By late embryogenesis, active secretion is evident by light and transmission electron microsc ...
pdf, 1.1 MB - The Nebenführ Lab
... persists until completion of cytokinesis. Maintenance of the distinct localizations does not depend on intact actin filaments or microtubules, although the mitotic spindle appears to play a major role in organizing the organelle distribution patterns. The redistribution of Golgi stacks during mitosi ...
... persists until completion of cytokinesis. Maintenance of the distinct localizations does not depend on intact actin filaments or microtubules, although the mitotic spindle appears to play a major role in organizing the organelle distribution patterns. The redistribution of Golgi stacks during mitosi ...
Tissue engineering
Tissue engineering is the use of a combination of cells, engineering and materials methods, and suitable biochemical and physicochemical factors to improve or replace biological functions. While it was once categorized as a sub-field of biomaterials, having grown in scope and importance it can be considered as a field in its own right.While most definitions of tissue engineering cover a broad range of applications, in practice the term is closely associated with applications that repair or replace portions of or whole tissues (i.e., bone, cartilage, blood vessels, bladder, skin, muscle etc.). Often, the tissues involved require certain mechanical and structural properties for proper functioning. The term has also been applied to efforts to perform specific biochemical functions using cells within an artificially-created support system (e.g. an artificial pancreas, or a bio artificial liver). The term regenerative medicine is often used synonymously with tissue engineering, although those involved in regenerative medicine place more emphasis on the use of stem cells or progenitor cells to produce tissues.