Monitoring Growth of Beer Brewing Strains of
... from multiple points within the read chamber, providing uniform temperatures across the plate. An often overlooked point is evaporation and the need for plate sealers. Long kinetic assays such as these require a better means of evaporation prevention than can be provided with standard plate covers s ...
... from multiple points within the read chamber, providing uniform temperatures across the plate. An often overlooked point is evaporation and the need for plate sealers. Long kinetic assays such as these require a better means of evaporation prevention than can be provided with standard plate covers s ...
Millius et al., J. Cell Sci., 2012 - Cardiovascular Research Institute
... within 1 mm of the leading edge (Fig. 2G,H). The distribution of p40 might be more restricted than either actin or WAVE2 for several reasons: other Arp2/3 complex activators such as WASP or N-WASP in combination with WAVE2 might further bias Arp2/3 to the cell periphery, or undersampling of WAVE2 pa ...
... within 1 mm of the leading edge (Fig. 2G,H). The distribution of p40 might be more restricted than either actin or WAVE2 for several reasons: other Arp2/3 complex activators such as WASP or N-WASP in combination with WAVE2 might further bias Arp2/3 to the cell periphery, or undersampling of WAVE2 pa ...
Control of Cell Pattern in the Neural Tube: Motor Neuron Induction
... Neural crest cells were defined by their migratory properties, the surface expression of the HNK-1, PI integrin, and p75 antigens (Maxwell et al., 1988; Delannet and Duband, 1992; Bernd, 1985; Stemple and Anderson, 1992), and their ability to differentiate into neurons and melanocytes. Ventral neura ...
... Neural crest cells were defined by their migratory properties, the surface expression of the HNK-1, PI integrin, and p75 antigens (Maxwell et al., 1988; Delannet and Duband, 1992; Bernd, 1985; Stemple and Anderson, 1992), and their ability to differentiate into neurons and melanocytes. Ventral neura ...
Accepted version - QMRO Home - Queen Mary University of London
... Recent pre-clinical and clinical research has suggested that transplantation of bone marrowderived mesenchymal stromal cells (MSCs) is a promising new approach for the treatment of heart failure.[1] Although cardiomyogenic differentiation of these cells in vivo is limited, MSCs are able to induce th ...
... Recent pre-clinical and clinical research has suggested that transplantation of bone marrowderived mesenchymal stromal cells (MSCs) is a promising new approach for the treatment of heart failure.[1] Although cardiomyogenic differentiation of these cells in vivo is limited, MSCs are able to induce th ...
Protoplast isolation - student notes
... be cultured on media which induce cell division and differentiation. A large number of plants can be regenerated from a single experiment – a gram of potato leaf tissue can produce more than a million protoplasts, for example. Protoplasts can be isolated from a range of plant tissues: leaves, stems, ...
... be cultured on media which induce cell division and differentiation. A large number of plants can be regenerated from a single experiment – a gram of potato leaf tissue can produce more than a million protoplasts, for example. Protoplasts can be isolated from a range of plant tissues: leaves, stems, ...
Monopolar spindle attachment of sister chromatids is ensured by two
... Chromosomal behavior at meiosis I, however, was strikingly different. The two DNA masses formed through this division appeared to be equal in size (Figure 2C, a and d), and both contained the signal of the loci in most cells (Figure 2C, b, c, e and f, and D). This indicates that sister chromatids at ...
... Chromosomal behavior at meiosis I, however, was strikingly different. The two DNA masses formed through this division appeared to be equal in size (Figure 2C, a and d), and both contained the signal of the loci in most cells (Figure 2C, b, c, e and f, and D). This indicates that sister chromatids at ...
Anatomical Characterization and Cellular Physiology of Rat Aortic
... immunopositive for purinergic P2X2 and P2X3 receptor subunits, suggesting that ACh and ATP may act as neurotransmitters as in the related carotid body. In a novel dissociated AB culture model, subsets of type I cells exhibited elevated intracellular Ca2+ responses to hypoxia, isohydric hypercapnia, ...
... immunopositive for purinergic P2X2 and P2X3 receptor subunits, suggesting that ACh and ATP may act as neurotransmitters as in the related carotid body. In a novel dissociated AB culture model, subsets of type I cells exhibited elevated intracellular Ca2+ responses to hypoxia, isohydric hypercapnia, ...
Subcellular localization of peroxidase in tomato fruit skin and the
... fruits was con®rmed. Furthermore, peroxidase activity was also observed associated with the plasma membrane and large vesicles allied to the plasma membrane. While cross-linking in cell wall components was previously assumed to be the mechanism by which peroxidase might control fruit growth, the inc ...
... fruits was con®rmed. Furthermore, peroxidase activity was also observed associated with the plasma membrane and large vesicles allied to the plasma membrane. While cross-linking in cell wall components was previously assumed to be the mechanism by which peroxidase might control fruit growth, the inc ...
BSMB Spring Meeting York University 7-8 April 2008 Insights into Osteoarthritis
... When I'm sixty-four... : Young patients with old knees exploring early-stage OA with human models and biomarkers. ...
... When I'm sixty-four... : Young patients with old knees exploring early-stage OA with human models and biomarkers. ...
Chloroplast anchoring: its implications for the
... would not allow the chloroplasts to maintain their position within the cell, resulting in decreased light use efficiency. As described later, the concept of ‘chloroplast anchoring’ has arisen from careful analysis of chloroplast behaviour in various types of plant cells at different developmental st ...
... would not allow the chloroplasts to maintain their position within the cell, resulting in decreased light use efficiency. As described later, the concept of ‘chloroplast anchoring’ has arisen from careful analysis of chloroplast behaviour in various types of plant cells at different developmental st ...
The physical basis of active mechanosensitivity by the hair
... currents show no sign of fast adaptation or Ca2þ sensitivity [42]. Alternatively, a protein associated with the channel may change conformation [31] or reduce its stiffness [43] to relax tip-link tension and in turn allow channel reclosure. The involvement of myosin 1c in fast adaptation points to t ...
... currents show no sign of fast adaptation or Ca2þ sensitivity [42]. Alternatively, a protein associated with the channel may change conformation [31] or reduce its stiffness [43] to relax tip-link tension and in turn allow channel reclosure. The involvement of myosin 1c in fast adaptation points to t ...
IDETIFYIG GEES THAT REGULATE SECODARY GROWTH I POPLAR
... are responsible for vertical growth, in which many key players have been well studied. Lateral (secondary) growth is controlled by the vascular and cork cambiums, which are much less understood. A rapid growth of interest in a new model angiosperm tree, poplar, has facilitated the study of the two c ...
... are responsible for vertical growth, in which many key players have been well studied. Lateral (secondary) growth is controlled by the vascular and cork cambiums, which are much less understood. A rapid growth of interest in a new model angiosperm tree, poplar, has facilitated the study of the two c ...
BRAIN CCR2 Ly-6C monocytes are crucial for the
... antibodies against CCL2 had no effect on disease onset, but on the course of established experimental autoimmune EAE (Kennedy et al., 1998). Considering all these data, the CCR2/CCL2 axis could potentially represent an excellent target for the treatment of multiple sclerosis. However, despite growin ...
... antibodies against CCL2 had no effect on disease onset, but on the course of established experimental autoimmune EAE (Kennedy et al., 1998). Considering all these data, the CCR2/CCL2 axis could potentially represent an excellent target for the treatment of multiple sclerosis. However, despite growin ...
Sucrose and Invertase, an Uneasy Alliance
... Figure 3.1. Scheme showing the involvement of acid invertase in regulation of cellular hexose content. Fast exchanges are shown by thick arrows, and slow exchanges by thin arrows. Fru, fructose; glu, glucose; INV, invertase; TP, triose phosphate; *, symplastic export of sucrose; **, apoplastic expor ...
... Figure 3.1. Scheme showing the involvement of acid invertase in regulation of cellular hexose content. Fast exchanges are shown by thick arrows, and slow exchanges by thin arrows. Fru, fructose; glu, glucose; INV, invertase; TP, triose phosphate; *, symplastic export of sucrose; **, apoplastic expor ...
FKBP12-rapamycin-associated protein associates
... integrates multiple and occasionally conflicting signals to coordinate the response. FKBP12-rapamycin-associated protein (FRAP) plays an important role in this network and ensures that cell growth occurs under optimal conditions, in part by regulating a p70 S6 kinase (p70S6K) phosphatase (1). Rapamy ...
... integrates multiple and occasionally conflicting signals to coordinate the response. FKBP12-rapamycin-associated protein (FRAP) plays an important role in this network and ensures that cell growth occurs under optimal conditions, in part by regulating a p70 S6 kinase (p70S6K) phosphatase (1). Rapamy ...
Caveolae as potential macromolecule trafficking
... was visually enhanced by silver development. The very reflective particles of dense gold / silver caveolin-1 immunostain are shown in black. The images are shown in the grid in order left to right, top to bottom, starting from the uppermost surface of the paraffin section and moving through to the b ...
... was visually enhanced by silver development. The very reflective particles of dense gold / silver caveolin-1 immunostain are shown in black. The images are shown in the grid in order left to right, top to bottom, starting from the uppermost surface of the paraffin section and moving through to the b ...
Involvement of Native TRPC3 Proteins in ATP
... constitutive cation influx with 2 mmol/L Ca2⫹ in the bath likely reflects operation of a highly efficient Ca2⫹ buffering system. In line with this, when cells were exposed to higher Ca2⫹ gradients (10 mmol/L in the bath), a significant yet transient Ca2⫹ influx was observed (not shown). Ba2⫹ is not ...
... constitutive cation influx with 2 mmol/L Ca2⫹ in the bath likely reflects operation of a highly efficient Ca2⫹ buffering system. In line with this, when cells were exposed to higher Ca2⫹ gradients (10 mmol/L in the bath), a significant yet transient Ca2⫹ influx was observed (not shown). Ba2⫹ is not ...
the fine-tuning of the endomembrane system
... Laboratory of Molecular Biology, Graduate School Wageningen University, Droevendaalsesteeg 1, 6708PB ...
... Laboratory of Molecular Biology, Graduate School Wageningen University, Droevendaalsesteeg 1, 6708PB ...
Gene Deletion Screen for Cardiomyopathy in Adult Drosophila
... (suppressor of Hairless) transcription factor, resulting in the expression of specific target genes such as E(spl) (enhancer of split). This activation is strictly controlled, and deregulation causes extreme developmental defects.24 –27 The stringency of the control system is provided by the general ...
... (suppressor of Hairless) transcription factor, resulting in the expression of specific target genes such as E(spl) (enhancer of split). This activation is strictly controlled, and deregulation causes extreme developmental defects.24 –27 The stringency of the control system is provided by the general ...
Print
... Expression and Function of Laminins in the Embryonic and Mature Vasculature. Physiol Rev 85: 979 –1000, 2005; doi:10.1152/physrev.00014.2004.—Endothelial cells of the blood and lymphatic vasculature are polarized cells with luminal surfaces specialized to interact with inflammatory cells upon the ap ...
... Expression and Function of Laminins in the Embryonic and Mature Vasculature. Physiol Rev 85: 979 –1000, 2005; doi:10.1152/physrev.00014.2004.—Endothelial cells of the blood and lymphatic vasculature are polarized cells with luminal surfaces specialized to interact with inflammatory cells upon the ap ...
manipulation of respiratory burst of neutrophils using c1
... Figure 4-21 Effects of Triton X-100 on extracted SOD from WBC and EDTA. ................... 80 Figure 4-22 Effects of C1 on the activity of extracted SOD from PMN. (Subject 1) ............. 81 Figure 4-23 Effects of incubation temperature of C1 on activity of SOD. ............................. 82 Fi ...
... Figure 4-21 Effects of Triton X-100 on extracted SOD from WBC and EDTA. ................... 80 Figure 4-22 Effects of C1 on the activity of extracted SOD from PMN. (Subject 1) ............. 81 Figure 4-23 Effects of incubation temperature of C1 on activity of SOD. ............................. 82 Fi ...
Keysight Technologies Tensile Deformation of Fibers Used in Textile
... years, it has also been an important research direction to understand the structureproperty relations in the natural fiber materials, which then can be successfully mimicked in an artificial material. Given the importance of understanding mechanical behavior in textile industry it is reasonable to a ...
... years, it has also been an important research direction to understand the structureproperty relations in the natural fiber materials, which then can be successfully mimicked in an artificial material. Given the importance of understanding mechanical behavior in textile industry it is reasonable to a ...
Golgi-SNARE GS28 potentiates cisplatin
... as well as the pro-apoptotic phosphorylation of p53 on Ser46 . Further experiments showed that these cellular responses could be ...
... as well as the pro-apoptotic phosphorylation of p53 on Ser46 . Further experiments showed that these cellular responses could be ...
Quantitative analysis of changes in spatial distribution and plus
... types are shown as colored dots (yellow, blunt ends; green, extended ends; red, horned ends; blue, flared ends). The high proportion of yellow dots associated with the solid phragmoplast cell-plate assembly matrix (Fig. 3B) illustrates that the association of a microtubule plus end with the cellplat ...
... types are shown as colored dots (yellow, blunt ends; green, extended ends; red, horned ends; blue, flared ends). The high proportion of yellow dots associated with the solid phragmoplast cell-plate assembly matrix (Fig. 3B) illustrates that the association of a microtubule plus end with the cellplat ...
Effects of phytanic acid on the vitamin E status, lipid composition and
... of myelin destabilization due to incorporation of phytanic acid into its structure [7]. An accumulation of phytanic acid in the retina may, therefore, perturb retinal membrane functions, such as that of the photoreceptor outer membranes or the apical membranes of the retinal pigment epithelium (RPE) ...
... of myelin destabilization due to incorporation of phytanic acid into its structure [7]. An accumulation of phytanic acid in the retina may, therefore, perturb retinal membrane functions, such as that of the photoreceptor outer membranes or the apical membranes of the retinal pigment epithelium (RPE) ...
Tissue engineering
Tissue engineering is the use of a combination of cells, engineering and materials methods, and suitable biochemical and physicochemical factors to improve or replace biological functions. While it was once categorized as a sub-field of biomaterials, having grown in scope and importance it can be considered as a field in its own right.While most definitions of tissue engineering cover a broad range of applications, in practice the term is closely associated with applications that repair or replace portions of or whole tissues (i.e., bone, cartilage, blood vessels, bladder, skin, muscle etc.). Often, the tissues involved require certain mechanical and structural properties for proper functioning. The term has also been applied to efforts to perform specific biochemical functions using cells within an artificially-created support system (e.g. an artificial pancreas, or a bio artificial liver). The term regenerative medicine is often used synonymously with tissue engineering, although those involved in regenerative medicine place more emphasis on the use of stem cells or progenitor cells to produce tissues.