Probing for Binding Regions of the FtsZ Protein Surface through Site
... cell division. We concluded that these faces are not sites of essential interactions. Inserts at two sites, G124 and R174, located on the left and right surfaces, completely blocked function, and these sites were identified as possible sites for essential lateral interactions. However, the insert at ...
... cell division. We concluded that these faces are not sites of essential interactions. Inserts at two sites, G124 and R174, located on the left and right surfaces, completely blocked function, and these sites were identified as possible sites for essential lateral interactions. However, the insert at ...
Auxin and other signals on the move in plants
... of the plasmodesmata pores, which control the passage of materials through plasmodesmata based on molecular size40,41,44. Some proteins targeted for this type of transport can specifically adapt the exclusion limit to allow their own flow48. ...
... of the plasmodesmata pores, which control the passage of materials through plasmodesmata based on molecular size40,41,44. Some proteins targeted for this type of transport can specifically adapt the exclusion limit to allow their own flow48. ...
Responses to Cell Loss Become Restricted as the Supporting Cells
... The reinforcement of the junctions occurs contemporaneously with sharp postnatal declines in SC plasticity, SC proliferation, new HC production, and mitotic HC regeneration (Ruben, 1967; Gu et al., 1997, 2007; Davies et al., 2007; Meyers and Corwin, 2007; Oshima et al., 2007; Lu and Corwin, 2008; Bu ...
... The reinforcement of the junctions occurs contemporaneously with sharp postnatal declines in SC plasticity, SC proliferation, new HC production, and mitotic HC regeneration (Ruben, 1967; Gu et al., 1997, 2007; Davies et al., 2007; Meyers and Corwin, 2007; Oshima et al., 2007; Lu and Corwin, 2008; Bu ...
Logistics of water and salt transport through the plant
... swelling or shrinking of the vessel-to-vessel pit membranes’. Van Ieperen et al. (2000) concluded that the presence of small amounts of cations (even the effect of 10 µM KCl was significant) and not the osmotic strength influenced the conductance. However, their conclusion that swelling and shrinkin ...
... swelling or shrinking of the vessel-to-vessel pit membranes’. Van Ieperen et al. (2000) concluded that the presence of small amounts of cations (even the effect of 10 µM KCl was significant) and not the osmotic strength influenced the conductance. However, their conclusion that swelling and shrinkin ...
Cardiomyocyte Lineage Specification in€Adult Human Cardiac
... CARMEN, a long noncoding RNA associated with an enhancer located in the MIR-143/145 locus and target of NOTCH signaling. The CARMEN/MIR-145/143 axis represents, therefore, a promising target to favor production of cardiomyocytes in cell replacement therapies. (J Am Coll Cardiol Basic Trans Science 2 ...
... CARMEN, a long noncoding RNA associated with an enhancer located in the MIR-143/145 locus and target of NOTCH signaling. The CARMEN/MIR-145/143 axis represents, therefore, a promising target to favor production of cardiomyocytes in cell replacement therapies. (J Am Coll Cardiol Basic Trans Science 2 ...
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... 1969; Smiley & Koch, 1971, 1972; Mato, Smiley & Dixon, 1972; Holmstedt & Han, 1973), and certain in vitro recombination experiments have been interpreted as showing that this cell death is induced by the palatal mesenchyme immediately prior to the time of palatal fusion (Pourtois, 1972). In the pres ...
... 1969; Smiley & Koch, 1971, 1972; Mato, Smiley & Dixon, 1972; Holmstedt & Han, 1973), and certain in vitro recombination experiments have been interpreted as showing that this cell death is induced by the palatal mesenchyme immediately prior to the time of palatal fusion (Pourtois, 1972). In the pres ...
Biofilms are described as surface ... et al.,
... The primary stage for biofilm formation is the attachment of bacteria to a surface followed by proliferation of attached cells which leads to the accumulation of multilayer clusters of cells and glycocalix formation (EPS) (Shakeri et al., 2007). Biofilms are composed of bacteria, extracellular poly ...
... The primary stage for biofilm formation is the attachment of bacteria to a surface followed by proliferation of attached cells which leads to the accumulation of multilayer clusters of cells and glycocalix formation (EPS) (Shakeri et al., 2007). Biofilms are composed of bacteria, extracellular poly ...
Novel Mechanism of PTEN Regulation by Its
... Cells sense external chemical gradients and respond by moving toward the higher concentration of chemoattractant. Chemotaxis is important for a wide variety of physiological and pathological events such as axon guidance, immune response, wound healing, tissue morphogenesis, and carcinoma invasion. T ...
... Cells sense external chemical gradients and respond by moving toward the higher concentration of chemoattractant. Chemotaxis is important for a wide variety of physiological and pathological events such as axon guidance, immune response, wound healing, tissue morphogenesis, and carcinoma invasion. T ...
Target recognition at the tips of postsynaptic filopodia
... Precise partner recognition between axons and their target cells is essential for developing a functional nervous system (TessierLavigne and Goodman, 1996; Garrity and Zipursky, 1995; Benson et al., 2001). A traditional view is that presynaptic growth cones explore the target region by elaborating m ...
... Precise partner recognition between axons and their target cells is essential for developing a functional nervous system (TessierLavigne and Goodman, 1996; Garrity and Zipursky, 1995; Benson et al., 2001). A traditional view is that presynaptic growth cones explore the target region by elaborating m ...
Characterising Non-Structural Protein NS4 of African Horse Sickness Virus
... that each of the ten AHSV genome segments (Seg-1 to Seg-10) contains a single open reading frame (ORF), thereby encoding the seven structural proteins (VP1—VP7) that form the virion, and three non-structural proteins (NS1—NS3/NS3A) with other functions during viral replication [9]. The structure of ...
... that each of the ten AHSV genome segments (Seg-1 to Seg-10) contains a single open reading frame (ORF), thereby encoding the seven structural proteins (VP1—VP7) that form the virion, and three non-structural proteins (NS1—NS3/NS3A) with other functions during viral replication [9]. The structure of ...
LPAproducing enzyme PAPLA1 regulates hair follicle development
... follicle layers and marker expressions in follicular layers. (F–H) Immunofluorescent detection of PA-PLA1a in dorsal hair follicles. Nuclei were stained with DAPI. Specificity of anti-PA-PLA1a antibody was confirmed using PA-PLA1a/ hair follicle (F). K72 (G) and K75 (H) (detected with Alexa Fluor ...
... follicle layers and marker expressions in follicular layers. (F–H) Immunofluorescent detection of PA-PLA1a in dorsal hair follicles. Nuclei were stained with DAPI. Specificity of anti-PA-PLA1a antibody was confirmed using PA-PLA1a/ hair follicle (F). K72 (G) and K75 (H) (detected with Alexa Fluor ...
3D representation of the developing chick knee joint:
... involves integration across several tissues and physical/mechanical influences from neighbouring tissues are important. We describe the acquisition of shape in the chick knee joint in detail and show that by HH34 the joint already displays shape characteristics of the adult structure. Through imagin ...
... involves integration across several tissues and physical/mechanical influences from neighbouring tissues are important. We describe the acquisition of shape in the chick knee joint in detail and show that by HH34 the joint already displays shape characteristics of the adult structure. Through imagin ...
Enzymes That Scavenge Reactive Oxygen
... were incubated in 5 m ABA for up to 48 h (Fig. 1, A, C, and E). Treatment of aleurone layers with 5 m GA, on the other hand, brought about a decrease in the number of living cells (green fluorescence) and an increase in the number of dead cells (orange/red fluorescence; Fig. 1, B, D, and F). The e ...
... were incubated in 5 m ABA for up to 48 h (Fig. 1, A, C, and E). Treatment of aleurone layers with 5 m GA, on the other hand, brought about a decrease in the number of living cells (green fluorescence) and an increase in the number of dead cells (orange/red fluorescence; Fig. 1, B, D, and F). The e ...
Dua`s Layer: its discovery, characteristics and applications
... BB to the limbus. This indicated that the DL extended to the limbus but the zone between the edge of the BB and the limbus was firmly attached to the underlying stroma. This attachment could not be broken either by injection of air or by attempts to mechanically and physically peel of the DL from th ...
... BB to the limbus. This indicated that the DL extended to the limbus but the zone between the edge of the BB and the limbus was firmly attached to the underlying stroma. This attachment could not be broken either by injection of air or by attempts to mechanically and physically peel of the DL from th ...
AxyPrep Multisource Total RNA Miniprep Kit
... genomic DNA and achieve complete release of the cellular RNA. Throughout these protocols, homogenization is achieved by passing the lysate several times through a syringe needle or with a manual or motorized homogenizer or. Shearing the genomic DNA reduces the viscosity of the lysate and results in ...
... genomic DNA and achieve complete release of the cellular RNA. Throughout these protocols, homogenization is achieved by passing the lysate several times through a syringe needle or with a manual or motorized homogenizer or. Shearing the genomic DNA reduces the viscosity of the lysate and results in ...
Generation of the podocyte and tubular components
... mesonephros is much simpler in structure than the metanephros, being a linear organ with only a few nephrons per body segment, and thus easier to manipulate and analyze. The current study investigated the initial stages of podocyte and tubule specification in the avian mesonephros. It was found that ...
... mesonephros is much simpler in structure than the metanephros, being a linear organ with only a few nephrons per body segment, and thus easier to manipulate and analyze. The current study investigated the initial stages of podocyte and tubule specification in the avian mesonephros. It was found that ...
Functions of mammalian microRNA in innate immunity to microbial
... This enables a rapid response to a pre-defined array of foreign antigens or abnormal cell ...
... This enables a rapid response to a pre-defined array of foreign antigens or abnormal cell ...
A Comparison of the Oxidation of Clozapine and Olanzapine to
... Packard diode-array spectrophotometer (HP 8452A; Hewlett Packard, Palo Alto, CA) was used to determine the rate of oxidation of olanzapine by hypochlorite. Scanning of the reaction mixture was initiated immediately after the addition of NaOCl (40 ml, 5 mM aqueous solution) to a solution of the drug ...
... Packard diode-array spectrophotometer (HP 8452A; Hewlett Packard, Palo Alto, CA) was used to determine the rate of oxidation of olanzapine by hypochlorite. Scanning of the reaction mixture was initiated immediately after the addition of NaOCl (40 ml, 5 mM aqueous solution) to a solution of the drug ...
FOXO1 Plays an Essential Role in Apoptosis of Retinal
... at passage 2 or 3. Prior to assay cells were incubated in serumfree DMEM medium for 2 days. For all assays cells were tested at approximately 70%–80% confluence in serum-free medium. In some cases cells were preincubated for 2 h with or without signaling inhibitors purchased from Calbiochem (LaJolla ...
... at passage 2 or 3. Prior to assay cells were incubated in serumfree DMEM medium for 2 days. For all assays cells were tested at approximately 70%–80% confluence in serum-free medium. In some cases cells were preincubated for 2 h with or without signaling inhibitors purchased from Calbiochem (LaJolla ...
The 2015 Nurse`s Guide to The Wound Institute®
... Skin aging is a complex process in which most of the major changes occur in the dermal region. Aging skin is at an increased risk for injury because the skin is thinner and more fragile due to the declining height of the rete pegs or ridges which occurs throughout life, allowing for easier separatio ...
... Skin aging is a complex process in which most of the major changes occur in the dermal region. Aging skin is at an increased risk for injury because the skin is thinner and more fragile due to the declining height of the rete pegs or ridges which occurs throughout life, allowing for easier separatio ...
Cadaverine is Transported into Vibrio vulnificus Through its CadB in
... three times, yielding similar results; data shown are representatives of triplicate experiments. Cadaverine Determination The cadaverine level in culture medium was determined as described previously [6, 9, 17]. To determine the cellular cadaverine, approximately 105 colony-forming units (CFU) were ...
... three times, yielding similar results; data shown are representatives of triplicate experiments. Cadaverine Determination The cadaverine level in culture medium was determined as described previously [6, 9, 17]. To determine the cellular cadaverine, approximately 105 colony-forming units (CFU) were ...
View PDF - OMICS International
... remain unnoticed until puberty. Any functioning thyroid tissue found outside of the normal thyroid location is termed ectopic thyroid tissue. Ectopic tissue has also been noted in the mediastinum, heart, esophagus, and diaphragm. Lingual thyroid is the result of failure of descent of the thyroid fro ...
... remain unnoticed until puberty. Any functioning thyroid tissue found outside of the normal thyroid location is termed ectopic thyroid tissue. Ectopic tissue has also been noted in the mediastinum, heart, esophagus, and diaphragm. Lingual thyroid is the result of failure of descent of the thyroid fro ...
Generation of the podocyte and tubular components
... mesonephros is much simpler in structure than the metanephros, being a linear organ with only a few nephrons per body segment, and thus easier to manipulate and analyze. The current study investigated the initial stages of podocyte and tubule specification in the avian mesonephros. It was found that ...
... mesonephros is much simpler in structure than the metanephros, being a linear organ with only a few nephrons per body segment, and thus easier to manipulate and analyze. The current study investigated the initial stages of podocyte and tubule specification in the avian mesonephros. It was found that ...
Skin Graft Preservation
... change of cell wall and ultra-structures in the cells. Even death can occur by intracellular ice crystals growing to a certain degree during freezing. This damage is more serious in the case of quicker freezing. Solution damage causes cell damage by extracellular high-concentration solution because ...
... change of cell wall and ultra-structures in the cells. Even death can occur by intracellular ice crystals growing to a certain degree during freezing. This damage is more serious in the case of quicker freezing. Solution damage causes cell damage by extracellular high-concentration solution because ...
Tissue engineering
Tissue engineering is the use of a combination of cells, engineering and materials methods, and suitable biochemical and physicochemical factors to improve or replace biological functions. While it was once categorized as a sub-field of biomaterials, having grown in scope and importance it can be considered as a field in its own right.While most definitions of tissue engineering cover a broad range of applications, in practice the term is closely associated with applications that repair or replace portions of or whole tissues (i.e., bone, cartilage, blood vessels, bladder, skin, muscle etc.). Often, the tissues involved require certain mechanical and structural properties for proper functioning. The term has also been applied to efforts to perform specific biochemical functions using cells within an artificially-created support system (e.g. an artificial pancreas, or a bio artificial liver). The term regenerative medicine is often used synonymously with tissue engineering, although those involved in regenerative medicine place more emphasis on the use of stem cells or progenitor cells to produce tissues.