Receptor Regulation of the Volume-Sensitive Efflux of Taurine and
... be readily differentiated from that of taurine on the basis of its more limited dependence on Ca2⫹ availability and, to a lesser extent, PKC activity. Thus, in SH-SY5Y cells, although both osmolytes may exit via a common (or pharmacologically similar) channel(s), distinct biochemical requirements ex ...
... be readily differentiated from that of taurine on the basis of its more limited dependence on Ca2⫹ availability and, to a lesser extent, PKC activity. Thus, in SH-SY5Y cells, although both osmolytes may exit via a common (or pharmacologically similar) channel(s), distinct biochemical requirements ex ...
Neural induction in Xenopus requires early FGF signalling in
... Next, we wanted to address whether BMP inhibition in the presumptive non-neural ectoderm was sufficient for neural induction in vivo. As expected from previous works (Mizuseki et al., 1998; Munoz-Sanjuan and Brivanlou, 2002), BMP inhibition obtained by RNA injection of a dominant-negative BMP type I ...
... Next, we wanted to address whether BMP inhibition in the presumptive non-neural ectoderm was sufficient for neural induction in vivo. As expected from previous works (Mizuseki et al., 1998; Munoz-Sanjuan and Brivanlou, 2002), BMP inhibition obtained by RNA injection of a dominant-negative BMP type I ...
Receptor Regulation of the Volume-Sensitive Efflux of Taurine and
... be readily differentiated from that of taurine on the basis of its more limited dependence on Ca2⫹ availability and, to a lesser extent, PKC activity. Thus, in SH-SY5Y cells, although both osmolytes may exit via a common (or pharmacologically similar) channel(s), distinct biochemical requirements ex ...
... be readily differentiated from that of taurine on the basis of its more limited dependence on Ca2⫹ availability and, to a lesser extent, PKC activity. Thus, in SH-SY5Y cells, although both osmolytes may exit via a common (or pharmacologically similar) channel(s), distinct biochemical requirements ex ...
Contribution of microtubule growth polarity and flux to spindle
... or perpendicular to the spindle axis (xt-plot, Fig. 3A), from the time-lapse measurements (xyt). Visual inspection of movies and these constructed kymographs revealed that metaphase plant chromosomes (seen as a negative stain) maintained their positions without appreciable movements in x or y direct ...
... or perpendicular to the spindle axis (xt-plot, Fig. 3A), from the time-lapse measurements (xyt). Visual inspection of movies and these constructed kymographs revealed that metaphase plant chromosomes (seen as a negative stain) maintained their positions without appreciable movements in x or y direct ...
Arabidopsis Kinetochore Fiber-Associated MAP65-4
... attach the kinetochore MTs to a spindle pole body or a centrosome. Instead, pole focusing of the kinotochore fibers has been proposed to rely on minus end–directed motors that may form cross-links between individual MTs (Smirnova et al., 1998; Chen, 2002; Ambrose et al., 2005). Selective cross-linki ...
... attach the kinetochore MTs to a spindle pole body or a centrosome. Instead, pole focusing of the kinotochore fibers has been proposed to rely on minus end–directed motors that may form cross-links between individual MTs (Smirnova et al., 1998; Chen, 2002; Ambrose et al., 2005). Selective cross-linki ...
A Critical Role for Extracellular DNA in Dental Plaque Formation
... samples using specific antibodies and an ultrasensitive dye. In addition, the possible structural role ...
... samples using specific antibodies and an ultrasensitive dye. In addition, the possible structural role ...
Naturally Occurring Ligand Isoforms Receptor Binding and Function
... We used a comparative approach to identify the fetal liver tyrosine kinase 3 (flt3) ligand structure required for binding and function. Two conserved bovine flt3 ligand isoforms, which differ in a defined region within the extracellular domain, were identified and shown to be uniformly transcribed i ...
... We used a comparative approach to identify the fetal liver tyrosine kinase 3 (flt3) ligand structure required for binding and function. Two conserved bovine flt3 ligand isoforms, which differ in a defined region within the extracellular domain, were identified and shown to be uniformly transcribed i ...
Paramecium as a bioassay system for elucidation of cytotoxicity and
... cytotoxicity effect on both cellular properties in all experiments performed in this study. However, we have found strong cytotoxic effect of CNF in the cell division tests. In this test we used culture medium containing lettuce juice and nutritive bacteria as described in “Materials and Methods”. T ...
... cytotoxicity effect on both cellular properties in all experiments performed in this study. However, we have found strong cytotoxic effect of CNF in the cell division tests. In this test we used culture medium containing lettuce juice and nutritive bacteria as described in “Materials and Methods”. T ...
Cytokinin–auxin crosstalk
... Cytokinin and auxin have a crucial role in the control of this balance. It has recently been shown that cytokinin controls the rate of meristematic cell differentiation, thus contributing to the determination of the Arabidopsis root meristem size [4]. Application of cytokinin causes a decrease in ro ...
... Cytokinin and auxin have a crucial role in the control of this balance. It has recently been shown that cytokinin controls the rate of meristematic cell differentiation, thus contributing to the determination of the Arabidopsis root meristem size [4]. Application of cytokinin causes a decrease in ro ...
Chapter 4 - Neurasync
... Lamp2a monomers for further substrates (7). Another fate of Lamp2a may be its translocation into the lysosomal lumen, where it does not participate in CMA and can be degraded by cathepsin A. Lysosomal (lys) Hsp90 stabilizes the transition phases of Lamp2a from monomeric to multimeric forms and vice ...
... Lamp2a monomers for further substrates (7). Another fate of Lamp2a may be its translocation into the lysosomal lumen, where it does not participate in CMA and can be degraded by cathepsin A. Lysosomal (lys) Hsp90 stabilizes the transition phases of Lamp2a from monomeric to multimeric forms and vice ...
Anti-HIV-1 activity of Trim 37
... Trim 37 reduced HIV-1 infectivity by approximately 10fold compared with virus produced in its absence as assessed in HeLa cells (Fig. 2a). We confirmed by RT-PCR that the increase in Trim 37 mRNA levels correlated with the amount of the Trim 37 plasmid transiently transfected into 293T cells (Fig. 2 ...
... Trim 37 reduced HIV-1 infectivity by approximately 10fold compared with virus produced in its absence as assessed in HeLa cells (Fig. 2a). We confirmed by RT-PCR that the increase in Trim 37 mRNA levels correlated with the amount of the Trim 37 plasmid transiently transfected into 293T cells (Fig. 2 ...
The paroxysm of Plasmodium vivax malaria
... The paroxysms of Plasmodium vivax malaria typically recur at intervals of 48 h. It has been known for over a century that malarial paroxysms coincide with the rupture of malaria parasite schizont-infected red blood cells in a patients’ circulation [1]. Schizont rupture exposes parasite material to t ...
... The paroxysms of Plasmodium vivax malaria typically recur at intervals of 48 h. It has been known for over a century that malarial paroxysms coincide with the rupture of malaria parasite schizont-infected red blood cells in a patients’ circulation [1]. Schizont rupture exposes parasite material to t ...
CHAPTER 2 Literature Review I Bone Homeostasis
... osteoblasts can be diverted into the adipocytic lineage and are then no longer available for bone formation. Factors driving MSCs to differentiate down the two lineages therefore play important roles in determining bone density. Although there is sufficient evidence for the theory that multipotent b ...
... osteoblasts can be diverted into the adipocytic lineage and are then no longer available for bone formation. Factors driving MSCs to differentiate down the two lineages therefore play important roles in determining bone density. Although there is sufficient evidence for the theory that multipotent b ...
Subcellular trafficking kinetics of GLUT4 mutated at the N
... case of TGN38, both a transmembrane domain and a β-turn type of motif [17,18]. GLUT4 has several potential targeting domains and evidence has been presented suggesting that all three types of motif may be involved in GLUT4 trafficking. Piper et al. [19] have shown that GLUT1}GLUT4 and asialoglycopro ...
... case of TGN38, both a transmembrane domain and a β-turn type of motif [17,18]. GLUT4 has several potential targeting domains and evidence has been presented suggesting that all three types of motif may be involved in GLUT4 trafficking. Piper et al. [19] have shown that GLUT1}GLUT4 and asialoglycopro ...
Molecular mapping of tyrosine-phosphorylated proteins in focal
... Integrin-mediated adhesion to the extracellular matrix (ECM) plays a pivotal role in the regulation of tissue integrity, cell migration and transmembrane signaling. These adhesions are formed in specialized sub-cellular sites, known as focal adhesions (Burridge and Chrzanowska-Wodnicka, 1996; Geiger ...
... Integrin-mediated adhesion to the extracellular matrix (ECM) plays a pivotal role in the regulation of tissue integrity, cell migration and transmembrane signaling. These adhesions are formed in specialized sub-cellular sites, known as focal adhesions (Burridge and Chrzanowska-Wodnicka, 1996; Geiger ...
The Young Mouse Heart Is Composed of Myocytes Heterogeneous
... The documentation that senescent and nonsenescent myocytes are present in the young adult mouse heart raised the question whether structural heterogeneity is paralleled by a similar variability in mechanical performance. Comparable results at the physiological level would support the notion that the ...
... The documentation that senescent and nonsenescent myocytes are present in the young adult mouse heart raised the question whether structural heterogeneity is paralleled by a similar variability in mechanical performance. Comparable results at the physiological level would support the notion that the ...
Genetic analysis of leaf development in cotton
... In dicotyledonous leaves the cells of the outermost layer (LI or dermatogen) give rise exclusively to the single cell layered epidermis by dividing anticlinally (Esau, 1965). This may be deduced from the orientation of cell plates in this region early in development and also from chimeras, although ...
... In dicotyledonous leaves the cells of the outermost layer (LI or dermatogen) give rise exclusively to the single cell layered epidermis by dividing anticlinally (Esau, 1965). This may be deduced from the orientation of cell plates in this region early in development and also from chimeras, although ...
Serum Factors from Pseudoxanthoma Elasticum
... Immunostaining of 10-day-old cultures with antibodies specific to elastin and fibrillin 1 revealed that PXE fibroblasts maintained in the presence of 10% FBS produced an abundant network of elastic fibers (Figure 1b and d). The distribution pattern of these elastic fibers did not appreciably differ ...
... Immunostaining of 10-day-old cultures with antibodies specific to elastin and fibrillin 1 revealed that PXE fibroblasts maintained in the presence of 10% FBS produced an abundant network of elastic fibers (Figure 1b and d). The distribution pattern of these elastic fibers did not appreciably differ ...
Occurrence, types and distribution of calcium oxalate crystals in
... Although the shape, size and number of crystals show variations among taxa, they have been classified into five main groups based on their morphology: as prism, druses, styloids, raphides and crystal sand (Webb 1999). Various physical, chemical and biological parameters such as light, temperature, p ...
... Although the shape, size and number of crystals show variations among taxa, they have been classified into five main groups based on their morphology: as prism, druses, styloids, raphides and crystal sand (Webb 1999). Various physical, chemical and biological parameters such as light, temperature, p ...
Spatiotemporal Patterns of Expression of NGF and the Low
... mandible of an E15.5 embryo hybridized to ~75 MX=Rantisense riboprobe. Note the extensive ~75~~~ expression throughout the mesenchyme of the maxillary process. G, Dark-field photomicrograph of section adjacent to F and hybridized to NGF antisense riboprobe. Note transcripts are confined to the epith ...
... mandible of an E15.5 embryo hybridized to ~75 MX=Rantisense riboprobe. Note the extensive ~75~~~ expression throughout the mesenchyme of the maxillary process. G, Dark-field photomicrograph of section adjacent to F and hybridized to NGF antisense riboprobe. Note transcripts are confined to the epith ...
pdf - European Association of Tissue Banks
... Remarkable developments in the scientific, technical and medical fields have led to the increased therapeutic use of human organs, tissues and cells. Transplantation of these substances of human origin (SOHO) has not only improved the quality of life of thousands of individuals but also prolonged th ...
... Remarkable developments in the scientific, technical and medical fields have led to the increased therapeutic use of human organs, tissues and cells. Transplantation of these substances of human origin (SOHO) has not only improved the quality of life of thousands of individuals but also prolonged th ...
Gut microbiota translocation to the pancreatic lymph nodes
... The intestinal microbiota has also been considered an important component of T1D development (van Belle et al., 2011; Sorini and Falcone, 2013). However, whether it plays a protective or pathogenic role in the disease remains controversial. In this context, it has been shown that, in rodent models, ...
... The intestinal microbiota has also been considered an important component of T1D development (van Belle et al., 2011; Sorini and Falcone, 2013). However, whether it plays a protective or pathogenic role in the disease remains controversial. In this context, it has been shown that, in rodent models, ...
Organization of cardiac chamber progenitors in the zebrafish blastula
... cells are determined by morphology and location, either in whole mount (E,G,I-L) or in longitudinal sections (F,H). We show a series of typical results. (E) After labeling three blastomeres in tier 1, 105-115° from dorsal, we found six labeled cells in the ventricular myocardium (arrowhead). (F) Sec ...
... cells are determined by morphology and location, either in whole mount (E,G,I-L) or in longitudinal sections (F,H). We show a series of typical results. (E) After labeling three blastomeres in tier 1, 105-115° from dorsal, we found six labeled cells in the ventricular myocardium (arrowhead). (F) Sec ...
PDF + SI
... The gliotransmitter D-serine is released upon (S)-␣-amino-3-hydroxy5-methyl-4-isoxazolepropionic acid兾kainate and metabotropic glutamate receptor stimulation, but the mechanisms involved are unknown. Here, by using a highly sensitive bioassay to continuously monitor extracellular D-serine levels, we ...
... The gliotransmitter D-serine is released upon (S)-␣-amino-3-hydroxy5-methyl-4-isoxazolepropionic acid兾kainate and metabotropic glutamate receptor stimulation, but the mechanisms involved are unknown. Here, by using a highly sensitive bioassay to continuously monitor extracellular D-serine levels, we ...
refinement of an in vitro cell model for cytochrome p450 induction
... greatly facilitate the ability to develop drugs devoid of these possible negative traits. Such a model would offer the advantage of requiring less drug (,1 mg) and reducing the number of animals used. In addition, confounding factors such as bioavailability, blood, and liver levels of the drug would ...
... greatly facilitate the ability to develop drugs devoid of these possible negative traits. Such a model would offer the advantage of requiring less drug (,1 mg) and reducing the number of animals used. In addition, confounding factors such as bioavailability, blood, and liver levels of the drug would ...
Tissue engineering
Tissue engineering is the use of a combination of cells, engineering and materials methods, and suitable biochemical and physicochemical factors to improve or replace biological functions. While it was once categorized as a sub-field of biomaterials, having grown in scope and importance it can be considered as a field in its own right.While most definitions of tissue engineering cover a broad range of applications, in practice the term is closely associated with applications that repair or replace portions of or whole tissues (i.e., bone, cartilage, blood vessels, bladder, skin, muscle etc.). Often, the tissues involved require certain mechanical and structural properties for proper functioning. The term has also been applied to efforts to perform specific biochemical functions using cells within an artificially-created support system (e.g. an artificial pancreas, or a bio artificial liver). The term regenerative medicine is often used synonymously with tissue engineering, although those involved in regenerative medicine place more emphasis on the use of stem cells or progenitor cells to produce tissues.