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Stem Cell Teachers` Kit
Stem Cell Teachers` Kit

... acquisition and use is commonly poorly understood by students and adults alike. This kit enables teachers to introduce and incorporate the concepts of stem cell science into their class rooms. What this kit contains: The kit consists of six chapters (see contents below), each chapter containing a nu ...
Cell fate specification and polarisation in mouse preimplantation
Cell fate specification and polarisation in mouse preimplantation

... This thesis may be made available for consultation within the University Library and may be photocopied or lent to other libraries for the purposes of consultation with effect from ………………….. Signed on behalf of the Faculty of Science …………………….. ...
The 21-day postnatal rat ventricular cardiac muscle cell in culture as
The 21-day postnatal rat ventricular cardiac muscle cell in culture as

... study the mechanism(s) by which cardiac muscle cells permanently exit the cell cycle during early neonatal life. Ventricular cardiomyocytes, isolated by retrograde perfusion of hearts from 21-day-old and adult rats, were compared through 10 days of culture. Expression patterns of genes encoding deve ...
Cleavage modification did not alter early blastomere fates
Cleavage modification did not alter early blastomere fates

... bioRxiv preprint first posted online Aug. 11, 2016; doi: http://dx.doi.org/10.1101/068783. The copyright holder for this preprint (which was not peer-reviewed) is the author/funder. It is made available under a CC-BY 4.0 International license. ...
PureYield™ RNA Midiprep System Technical Manual
PureYield™ RNA Midiprep System Technical Manual

... The PureYield™ RNA Midiprep System(a) is designed to quickly and easily isolate high yields of pure total RNA from essentially any sample type and virtually eliminates the copurification of DNA (1). The streamlined, universal protocol uses the PureYield™ silica-membrane technology to isolate intact ...
Full Text  - The International Journal of Developmental Biology
Full Text - The International Journal of Developmental Biology

... inducers act permissively, because they are not unique to the organizer, and because multipotent responsive cells develop complex local differentiations under artificial conditions. Furthermore, with the discovery of meso-endoderm induction in 1969, the possibility arose that this earlier induction ...
the extracellular electrical current pattern and its variability in
the extracellular electrical current pattern and its variability in

... Interestingly, the inward-directed current at the posterior pole of late vitellogenic follicles can already be detected at stages 9—11 in a small fraction of analysed follicles. At stage 12 we observed inward or outward current, or no current, with the same probability (Figs 2c, 3m,n). At the final ...
Cytological Fixation by Chromic Acid and Dichromates By W. G.
Cytological Fixation by Chromic Acid and Dichromates By W. G.

... contents, resisted only by the nucleolus, and (2) with potassium dichromate and the other less acidic dichromates (ammonium, lithium, or sodium), the mitochondria, cytoplasm, and nucleus are well fixed. With various tissues from mice, the transition between the two types occurs around pH 3-4-3-8. Th ...
Functional Analysis of Class 1 RSL Genes in Caulonema and
Functional Analysis of Class 1 RSL Genes in Caulonema and

... caulonema. The first cells to develop from the spore are chloronema (Cove and Knight, 1993). Chloronema cells are short and contain numerous chloroplasts and perpendicular cell walls, compared to caulonema. The numerous well-developed chloroplasts are very similar to those of leaf cells in ultrastru ...
Mutations in the Catalytic Domain of Prohormone Convertase 2
Mutations in the Catalytic Domain of Prohormone Convertase 2

... residues within the peptide are also critical to inhibitory potency (44). No information is available thus far on which PC2 residues confer the ability to be inhibited by the CT peptide; presumably, these residues are not shared by PC1, which is not inhibited by this peptide (45). In contrast to the ...
Daughter-Specific Transcription Factors Regulate Cell Size Control
Daughter-Specific Transcription Factors Regulate Cell Size Control

... means that the mother cell feeds less biomass into the daughter cell, resulting in smaller daughter size at the time of cell division [3]. The resulting population of very small daughters enhances detection of size control (Figure 1C) [5]. In glycerol-ethanol, across the domain of size overlap in mo ...
Involvement of CD14 and Complement Receptors CR3 and CR4 in
Involvement of CD14 and Complement Receptors CR3 and CR4 in

... LPS and GBS induce TNF production from human monocytes in a CD14-dependent manner, and human serum, LBP, and sCD14 enhance the response In the first series of experiments, we compared the capacities of LPS and GBS to induce TNF from human monocytes and evaluated the roles of membrane and sCD14 as we ...
Lymph heart in chick – somitic origin, development and embryonic
Lymph heart in chick – somitic origin, development and embryonic

... determined which specific part of the somite contributes to the lymph heart. We substituted the dorsal portion of the newly formed somite I (at the position of somite 37) of the chick host with corresponding tissue of quail origin (Fig. 2A). These experiments showed that quail cells contributed to t ...
Transforming growth factor–1 in supernatants from stored red blood
Transforming growth factor–1 in supernatants from stored red blood

... Consistent with the possible increased risk of postoperative infections in transfusion patients, various studies suggest that transfusions can induce immunomodulatory effects, including transient reduction in the CD4/CD8 T-cell ratio, reduced natural killer function, impaired lymphocyte mitogenic re ...
Robust methods for purification of histones from cultured
Robust methods for purification of histones from cultured

... procedures, which use strong protein denaturing conditions and yield highly purified core and linker histones from unsynchronized proliferating, M-phase arrested and butyrate-treated cells, fully preserving their native PTMs without using enzyme inhibitors. Cell hypotonic swelling and lysis, nuclei ...
The maxillary recess of the sphenoid sinus
The maxillary recess of the sphenoid sinus

... The most studied recess of the sphenoid sinus seems to be the lateral one, which is highly variable. Less attention seems to be paid to the maxillary recess, extending anterior, inferior and lateral towards the maxillary sinus. Twenty patients were referred for CT of the paranasal sinuses (axial CT) ...
Functional Regulation of MyD88-Activated Interferon Regulatory
Functional Regulation of MyD88-Activated Interferon Regulatory

... Interferon regulatory factor 5 (IRF-5) plays an important role in the innate antiviral and inflammatory response. Specific IRF-5 haplotypes are associated with dysregulated expression of type I interferons and predisposition to autoimmune disorders. IRF-5 is activated by Toll-like receptor 7 (TLR7) ...
Argon Plasma Coagulation - Erbe Elektromedizin GmbH
Argon Plasma Coagulation - Erbe Elektromedizin GmbH

... in regards to tissue effect, compared to the technology of our older generation ICC Models. That said, the FORCED APC® mode in a VIO ESU/APC System is used particularly for hemostasis of small diffuse areas of bleeding, as well as the devitalization (i.e., depravity of life) and reduction of target ...
Calcium-induced calcium release supports recruitment of synaptic
Calcium-induced calcium release supports recruitment of synaptic

... mM) resulted in a net decrease in EPSC frequency that could be recovered upon washout (Fig. 2A). Change in frequency for nine fibers is presented in Fig. 2C. Of these, five are whole cell recordings and four are cell-attached recordings where spike rate could be monitored. In all cases the frequency ...
Disruption of morphogenesis and transformation of
Disruption of morphogenesis and transformation of

... suspensor may consist of a single cell or more than 100 cells, and it may be small or large relative to the embryo proper. Suspensors typically are filamentous, columnar, spherical, or irregular in shape, and the boundary between the suspensor and embryo proper can be distinct or diffuse. Following ...
Pharmacol Rev.2006;58(2):194-243
Pharmacol Rev.2006;58(2):194-243

... the pancreas leads to severe pancreatitis, with accompanying pain and tissue destruction (Morrow et al., 1984). Islet transplantation can provide certain advantages that are not available with pancreas transplantation including the potential for modifying tissue immunogenicity through in vitro cultu ...
The Drosophila Planar Polarity Proteins Inturned and
The Drosophila Planar Polarity Proteins Inturned and

... particularly in the PPE mutant clones (Figure 1 C-H). Our results are consistent with those reported by (STRUTT and WARRINGTON 2008). We extended the analysis and quantified the decrease in two ways (see Methods for details). In the first we compared the mean fluorescence intensity for mutant clone ...
PDF
PDF

... et al., 2003; Schwend and Ahlgren, 2009). Although clearly crucial for vertebrate head development, how pouches achieve their stereotyped positions and morphologies remains poorly understood. Given the importance of pouches in craniofacial development, it is not surprising that mutations in genes th ...
Pretreatment Increases Cytokine-induced NO Production
Pretreatment Increases Cytokine-induced NO Production

... (3), which produce nanomolar amounts of NO. A third isoform, inducible NOS (iNOS) is expressed in response to inflammatory cytokines in many cell types including hepatocytes (4), and produces micromolar levels of NO. All of these isotypes are active as homodimers and require NADPH, FAD, FMN, calmodu ...
Chemotactic Effect of Ciliary Neurotrophic Factor on Macrophages in
Chemotactic Effect of Ciliary Neurotrophic Factor on Macrophages in

... microglia in neural damage and repair have been shown. Macrophages are often seen to associate with neurodegenerative diseases,16,17 but these cells can also be neuroprotective.18 The differential effects of macrophages under different conditions may be accounted for by the different phenotypes of t ...
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Tissue engineering



Tissue engineering is the use of a combination of cells, engineering and materials methods, and suitable biochemical and physicochemical factors to improve or replace biological functions. While it was once categorized as a sub-field of biomaterials, having grown in scope and importance it can be considered as a field in its own right.While most definitions of tissue engineering cover a broad range of applications, in practice the term is closely associated with applications that repair or replace portions of or whole tissues (i.e., bone, cartilage, blood vessels, bladder, skin, muscle etc.). Often, the tissues involved require certain mechanical and structural properties for proper functioning. The term has also been applied to efforts to perform specific biochemical functions using cells within an artificially-created support system (e.g. an artificial pancreas, or a bio artificial liver). The term regenerative medicine is often used synonymously with tissue engineering, although those involved in regenerative medicine place more emphasis on the use of stem cells or progenitor cells to produce tissues.
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