Effects of nitric oxide on red blood cell deformability
... cell contaminants. The cells were washed twice in PBS and once in HEPES buffer [containing (in mM) 123 NaCl, 5 KCl, 1 MgCl2 䡠 6H2O, 1.3 CaCl2, 10 glucose, and 25 HEPES; pH ⫽ 7.4] and then resuspended in the HEPES buffer at a hematocrit of 40%. After the addition of the NO donor SNP or the nonspecifi ...
... cell contaminants. The cells were washed twice in PBS and once in HEPES buffer [containing (in mM) 123 NaCl, 5 KCl, 1 MgCl2 䡠 6H2O, 1.3 CaCl2, 10 glucose, and 25 HEPES; pH ⫽ 7.4] and then resuspended in the HEPES buffer at a hematocrit of 40%. After the addition of the NO donor SNP or the nonspecifi ...
Regulation of plasminogen activator inhibitor 1 expression in human
... largely unknown. Cartilage is a supporting connective tissue com2350 ...
... largely unknown. Cartilage is a supporting connective tissue com2350 ...
b-Arrestin1 and Distinct CXCR4 Structures Are Required for Stromal
... into clathrin-coated pits on the plasma membrane followed by membrane invagination and then the pinching off of an endosome and the trafficking of the receptors into early endosomes. Following endocytosis, additional CXCR4 trafficking results in either receptor degradation or recycling back to the c ...
... into clathrin-coated pits on the plasma membrane followed by membrane invagination and then the pinching off of an endosome and the trafficking of the receptors into early endosomes. Following endocytosis, additional CXCR4 trafficking results in either receptor degradation or recycling back to the c ...
Physiological interactions between a mesophilic cellulolytic
... as these cellulose-derived sugars are formed extracellularly, they are available as fermentable substrates not only to the cellulolytic bacteria that generate them, but also to non-cellulolytic bacteria. Plant cell walls, and plant material in general, usually have a high C : N ratio and, therefore, ...
... as these cellulose-derived sugars are formed extracellularly, they are available as fermentable substrates not only to the cellulolytic bacteria that generate them, but also to non-cellulolytic bacteria. Plant cell walls, and plant material in general, usually have a high C : N ratio and, therefore, ...
Spemann`s organizer and the self-regulation of
... for this, which is that dorsal organizer genes can induce the formation of new structures such as heads or trunks, while overexpression of ventral genes in general causes defects in dorsal or head structures. Loss of structures can also be triggered by non-specific effects, and therefore ventral gen ...
... for this, which is that dorsal organizer genes can induce the formation of new structures such as heads or trunks, while overexpression of ventral genes in general causes defects in dorsal or head structures. Loss of structures can also be triggered by non-specific effects, and therefore ventral gen ...
Study of Pseudomonas aeruginosa and different wound dressing
... proliferative phase. This features a continuous influx of neutrophils into the wound area, which release free radicals, cytolytic enzymes and inflammatory mediators that cause damage to invading pathogens as well as to host tissue (Sibbald et al 2003). Other leukocytes present produce inflammatory m ...
... proliferative phase. This features a continuous influx of neutrophils into the wound area, which release free radicals, cytolytic enzymes and inflammatory mediators that cause damage to invading pathogens as well as to host tissue (Sibbald et al 2003). Other leukocytes present produce inflammatory m ...
Relationship of Net Chloride Flow across the Human Erythrocyte
... modification of Dalmark's method (1975b). The first portion (A) was washed in 75 mM KCI, 120 mM sucrose, and 5 mM HEPES, and then resuspended in the same solution (ice cold) at 10% hematocrit with 75 jig/ml nystatin (E. R. Squibb & Sons, Inc., Princeton, NJ) (added as a 5-mg/ml solution in methanol) ...
... modification of Dalmark's method (1975b). The first portion (A) was washed in 75 mM KCI, 120 mM sucrose, and 5 mM HEPES, and then resuspended in the same solution (ice cold) at 10% hematocrit with 75 jig/ml nystatin (E. R. Squibb & Sons, Inc., Princeton, NJ) (added as a 5-mg/ml solution in methanol) ...
Auxin Polar Transport 1s Essential for the
... The minimal concentration at which 100% of the globularstage embryos developed into mature embryos with fused cotyledons varied with the different inhibitors used, as shown in Figure 5. TIBA was the most effective of the three chemicals tested; only 2 u,M was required to induce cotyledon fusion. For ...
... The minimal concentration at which 100% of the globularstage embryos developed into mature embryos with fused cotyledons varied with the different inhibitors used, as shown in Figure 5. TIBA was the most effective of the three chemicals tested; only 2 u,M was required to induce cotyledon fusion. For ...
Anion-Channel Blockers Inhibit S-Type Anion
... prevent stomatal opening and promote stomatal closure by favoring loss of osmotically active anions and, simultaneously, promoting membrane depolarization (Schroeder and Hedrich, 1989). This has led to the hypothesis that anion-channel blockers might enhance stomatal opening and inhibit stomatal clo ...
... prevent stomatal opening and promote stomatal closure by favoring loss of osmotically active anions and, simultaneously, promoting membrane depolarization (Schroeder and Hedrich, 1989). This has led to the hypothesis that anion-channel blockers might enhance stomatal opening and inhibit stomatal clo ...
Full-Text PDF
... matrix in biofilms formed by different bacterial species [20]. The same is true for C. albicans, where the presence of eDNA has been described in the biofilm matrix under a variety of growing conditions [21], constituting around 5% of the weight of the matrix [10]. Proposed mechanisms implicated in ...
... matrix in biofilms formed by different bacterial species [20]. The same is true for C. albicans, where the presence of eDNA has been described in the biofilm matrix under a variety of growing conditions [21], constituting around 5% of the weight of the matrix [10]. Proposed mechanisms implicated in ...
Bostrom Slides
... Joint Diseases Connection between are “Crystal diseases” Vascular Calcification and Bone where synovial fibroblasts have an inflammatory response to the crystals. ...
... Joint Diseases Connection between are “Crystal diseases” Vascular Calcification and Bone where synovial fibroblasts have an inflammatory response to the crystals. ...
Phloem loading and unloading of sugars and amino acids
... between these regions. For terrestrial macrophytes, distances separating these assimilatory organs exceeded the capacity of simple diffusion to deliver nutrients at rates sufficient to meet demands for their cellular maintenance and growth. In vascular plants, these requirements were met by evolving ...
... between these regions. For terrestrial macrophytes, distances separating these assimilatory organs exceeded the capacity of simple diffusion to deliver nutrients at rates sufficient to meet demands for their cellular maintenance and growth. In vascular plants, these requirements were met by evolving ...
Amino acid regulation of mTORC1 Liron Bar-Peled
... some human cancer cell lines. This spurred initial interest in its application as a chemotherapeutic- an idea that would only be realized decades later in the treatment of advanced renal cell carcinoma (5, 6). Notably, rapamycin treatment of a variety of cell types resulted in a dramatic reduction ...
... some human cancer cell lines. This spurred initial interest in its application as a chemotherapeutic- an idea that would only be realized decades later in the treatment of advanced renal cell carcinoma (5, 6). Notably, rapamycin treatment of a variety of cell types resulted in a dramatic reduction ...
Development of the lymphatic system: new questions and paradigms
... system and provide a thorough historical analysis of the evolution of this field of research, with a special focus on the animal models and experimental approaches utilized. Finally, we discuss exciting findings from the past year highlighting novel potential sources for lymphatic endothelial cells. ...
... system and provide a thorough historical analysis of the evolution of this field of research, with a special focus on the animal models and experimental approaches utilized. Finally, we discuss exciting findings from the past year highlighting novel potential sources for lymphatic endothelial cells. ...
Vibrio cholerae 01 Can Assume a Chlorine
... Inaba N16961 developed symptoms typical of cholera, with a mean diarrheal stool volume of 2.2 L (range, 1.4-4.3). Isolates recovered from the stool of infected volunteers retained the rugose phenotype. The data suggest that rugose strains cause human disease. The role of these strains in the epidemi ...
... Inaba N16961 developed symptoms typical of cholera, with a mean diarrheal stool volume of 2.2 L (range, 1.4-4.3). Isolates recovered from the stool of infected volunteers retained the rugose phenotype. The data suggest that rugose strains cause human disease. The role of these strains in the epidemi ...
Induction of Apoptosis through B-cell Receptor Cross-linking
... AICD, the contribution of either and the exact molecular events through which AICD commences are still unknown. Here we show that in Ramos B-cells, BcR-triggered cell death is associated with an early rise of C16 ceramide that derives from activation of the de novo pathway, as demonstrated using a s ...
... AICD, the contribution of either and the exact molecular events through which AICD commences are still unknown. Here we show that in Ramos B-cells, BcR-triggered cell death is associated with an early rise of C16 ceramide that derives from activation of the de novo pathway, as demonstrated using a s ...
Calcium homeostasis in plants
... allows plant biologists to select indicators that are compatible with windows in the autofluorescence spectrum of their cells. With single-wavelength dyes, such as Fluo-3 or Calcium Green, the intensity of fluorescence increases with the concentration of free Ca2+. Calibration of these dyes is compl ...
... allows plant biologists to select indicators that are compatible with windows in the autofluorescence spectrum of their cells. With single-wavelength dyes, such as Fluo-3 or Calcium Green, the intensity of fluorescence increases with the concentration of free Ca2+. Calibration of these dyes is compl ...
Ligament Injury and Healing: A Review of Current Clinical
... Ligaments heal through a distinct sequence of cellular events that take place in three consecutive stages: an acute inflammatory phase, a proliferative or regenerative phase, and a tissue remodeling phase. The process can take months to resolve itself, and despite advances in therapeutics, many liga ...
... Ligaments heal through a distinct sequence of cellular events that take place in three consecutive stages: an acute inflammatory phase, a proliferative or regenerative phase, and a tissue remodeling phase. The process can take months to resolve itself, and despite advances in therapeutics, many liga ...
Cytokinins regulate vascular morphogenesis in the Arabidopsis
... terrestrial plants were able to cope with occasional drying, when growing on streams or mudflats, but they were still tightly depended on living on water. To successfully accomplish the transition from aquatic to terrestrial life, several structural and functional changes had to occur in plants. Ada ...
... terrestrial plants were able to cope with occasional drying, when growing on streams or mudflats, but they were still tightly depended on living on water. To successfully accomplish the transition from aquatic to terrestrial life, several structural and functional changes had to occur in plants. Ada ...
Extrapolation of elementary rate constants of P
... It is possible that more than one transporter is involved at these membranes. In that case, we would be fitting some weighted average. Importantly, P-gp mediated transport was not estimated by simply subtracting the transport in the presence of GF120918 from the total transport, which does not take ...
... It is possible that more than one transporter is involved at these membranes. In that case, we would be fitting some weighted average. Importantly, P-gp mediated transport was not estimated by simply subtracting the transport in the presence of GF120918 from the total transport, which does not take ...
12 October 2000
... this molecular 'murder mystery'. On page 777 Andrew Wyllie and co-workers discuss why and how DNA damage results in apoptosis in some cells but not in others and what the consequences are if cells with damaged genomes fail to die. Once a cell is committed to die, its corpse must be removed and destr ...
... this molecular 'murder mystery'. On page 777 Andrew Wyllie and co-workers discuss why and how DNA damage results in apoptosis in some cells but not in others and what the consequences are if cells with damaged genomes fail to die. Once a cell is committed to die, its corpse must be removed and destr ...
the article
... mechanisms by which ODNs are delivered to subcellular structures are unknown. TLR9 was originally believed to uniquely recognize bacterial or viral DNA, although it is now appreciated that TLR9 also recognizes unmethylated CpG–containing DNA sequences, including those of mammalian origin9,14. Howeve ...
... mechanisms by which ODNs are delivered to subcellular structures are unknown. TLR9 was originally believed to uniquely recognize bacterial or viral DNA, although it is now appreciated that TLR9 also recognizes unmethylated CpG–containing DNA sequences, including those of mammalian origin9,14. Howeve ...
A bi-functional siRNA construct induces RNA
... In contrast, crook siRNA quantification is both direct and represents a constant for different siRNAs since detection is reliant upon the DNA component of the crook siRNA construct (Figure 1). The siRNA component of crook siRNA can be varied according to the chosen gene target. Moreover our non-radi ...
... In contrast, crook siRNA quantification is both direct and represents a constant for different siRNAs since detection is reliant upon the DNA component of the crook siRNA construct (Figure 1). The siRNA component of crook siRNA can be varied according to the chosen gene target. Moreover our non-radi ...
Relative Potencies of Individual Polycyclic Aromatic Hydrocarbons to Induce Dioxinlike
... Mass-, or potency-, balance analysis is one approach for addressing whether the known composition of a chemical mixture, as measured by instrumental analysis, can account for the potency and/or the magnitude of the biological response observed (Sanderson and Giesy, 1998). Until now, mass-balance stu ...
... Mass-, or potency-, balance analysis is one approach for addressing whether the known composition of a chemical mixture, as measured by instrumental analysis, can account for the potency and/or the magnitude of the biological response observed (Sanderson and Giesy, 1998). Until now, mass-balance stu ...
Guard cell photosynthesis and stomatal function
... activity contributes to stomatal regulation. However, this is only one of four possible functions of guard cell chloroplasts; other roles include supply of ATP, blue-light signalling and starch storage. Evidence exists for all these mechanisms, but is highly dependent upon species and growth/measure ...
... activity contributes to stomatal regulation. However, this is only one of four possible functions of guard cell chloroplasts; other roles include supply of ATP, blue-light signalling and starch storage. Evidence exists for all these mechanisms, but is highly dependent upon species and growth/measure ...
Tissue engineering
Tissue engineering is the use of a combination of cells, engineering and materials methods, and suitable biochemical and physicochemical factors to improve or replace biological functions. While it was once categorized as a sub-field of biomaterials, having grown in scope and importance it can be considered as a field in its own right.While most definitions of tissue engineering cover a broad range of applications, in practice the term is closely associated with applications that repair or replace portions of or whole tissues (i.e., bone, cartilage, blood vessels, bladder, skin, muscle etc.). Often, the tissues involved require certain mechanical and structural properties for proper functioning. The term has also been applied to efforts to perform specific biochemical functions using cells within an artificially-created support system (e.g. an artificial pancreas, or a bio artificial liver). The term regenerative medicine is often used synonymously with tissue engineering, although those involved in regenerative medicine place more emphasis on the use of stem cells or progenitor cells to produce tissues.