Dramatic increase in lymph node dendritic cell
... A DC-enriched cell fraction was then obtained after depletion of T cells, B cells, and granulocytes by treating the 1.061-density fraction for 50 minutes at 4°C with a monoclonal antibody (mAb) mixture including anti-CD3 (clone KT3-1.1), anti-B220 (clone RA3-6B2), and antigranulocyte antigen Gr1 (cl ...
... A DC-enriched cell fraction was then obtained after depletion of T cells, B cells, and granulocytes by treating the 1.061-density fraction for 50 minutes at 4°C with a monoclonal antibody (mAb) mixture including anti-CD3 (clone KT3-1.1), anti-B220 (clone RA3-6B2), and antigranulocyte antigen Gr1 (cl ...
Activation of ryanodine receptors induces calcium influx in a
... we have examined the hypothesis that the 401L cell requires activation of RyRs to induce Ca2+ influx, a role described for RyRs in other neuronal cell types [9,10]. In the present study we report, using RT (reverse transcriptase)–PCR, the expression of mRNAs encoding two RyR isoforms (RyR1 and RyR2) ...
... we have examined the hypothesis that the 401L cell requires activation of RyRs to induce Ca2+ influx, a role described for RyRs in other neuronal cell types [9,10]. In the present study we report, using RT (reverse transcriptase)–PCR, the expression of mRNAs encoding two RyR isoforms (RyR1 and RyR2) ...
Survival in Animal Cells Requires ppGpp for Internalization and
... Koch’s lysis solution (51). For determinations of -galactosidase levels in bacteria at different stages of growth, bacteria cultured overnight were diluted 1:50 into medium with or without antibiotics as described in the text. Cultures were incubated further at 37°C to stationary phase. Samples wer ...
... Koch’s lysis solution (51). For determinations of -galactosidase levels in bacteria at different stages of growth, bacteria cultured overnight were diluted 1:50 into medium with or without antibiotics as described in the text. Cultures were incubated further at 37°C to stationary phase. Samples wer ...
Composition and production of thiol constituents induced
... ECnG). Phytochelatins with nranging from 2 to 11 have been described [4,5]. Although glycine is the most common terminal amino acid, glutamic acid [6], serine [7], and βalanine [8] have been found instead of glycine in several species. Moreover, the terminal amino acid does not appear in desglycyl- ...
... ECnG). Phytochelatins with nranging from 2 to 11 have been described [4,5]. Although glycine is the most common terminal amino acid, glutamic acid [6], serine [7], and βalanine [8] have been found instead of glycine in several species. Moreover, the terminal amino acid does not appear in desglycyl- ...
Polyethyleneimine is an effective permeabilizer of Gram
... Briefly, the bacteria were cultivated to OD,,, 0-5,then the cells were deposited by centrifugation (1000 g, 10 min at room temperature) and suspended in a similar volume of 5 mM HEPES, pH 7-2. Samples (1.35ml) of this suspension were pipetted directly into the fluorometer cuvette, and fluorescence w ...
... Briefly, the bacteria were cultivated to OD,,, 0-5,then the cells were deposited by centrifugation (1000 g, 10 min at room temperature) and suspended in a similar volume of 5 mM HEPES, pH 7-2. Samples (1.35ml) of this suspension were pipetted directly into the fluorometer cuvette, and fluorescence w ...
pH-Sensitive Core-Shell Nanoparticles for Intracellular Drug Delivery
... on June, 2008 in Partial Fulfillment of the Requirements for the Degree of Doctor of Philosophy in Chemical Engineering at The Massachusetts Institute of Technology ...
... on June, 2008 in Partial Fulfillment of the Requirements for the Degree of Doctor of Philosophy in Chemical Engineering at The Massachusetts Institute of Technology ...
Study of the Biogenesis of the Golgi Apparatus in Plant Cells （植物
... however, it is impossible to investigate the stacked structure by using it. Although major progress in ...
... however, it is impossible to investigate the stacked structure by using it. Although major progress in ...
FGF signaling is essential for the early events in the development of
... development of nervous system and mesodermal tissues in chick embryo. Treatment of in vitro cultured embryos with exogenous, human recombinant FGF led to abnormalities in neural induction and development, notochord formation and somitogenesis as studied by gross morphology and histology. Overall gro ...
... development of nervous system and mesodermal tissues in chick embryo. Treatment of in vitro cultured embryos with exogenous, human recombinant FGF led to abnormalities in neural induction and development, notochord formation and somitogenesis as studied by gross morphology and histology. Overall gro ...
2 Cellulite Doris Hexsel ...
... Cellulite normally occurs in areas with fat accumulation, such as buttocks, thighs, flanks, abdomen, and upper legs [14, 30]. The characteristic appearance of cellulite is the presence of depressions on the cutaneous surface, surrounded or not by elevations. The depressions can be deep or superficia ...
... Cellulite normally occurs in areas with fat accumulation, such as buttocks, thighs, flanks, abdomen, and upper legs [14, 30]. The characteristic appearance of cellulite is the presence of depressions on the cutaneous surface, surrounded or not by elevations. The depressions can be deep or superficia ...
Strategies of aerobic ammonia-oxidizing bacteria
... maintenance energy demand and decay rate, cell-to-cell communication, cell mobility, stable enzymes and RNAs, could allow AOB to maintain themselves under unfavourable circumstances. This review examines whether AOB possess such traits and how these traits might offer advantages over competing organ ...
... maintenance energy demand and decay rate, cell-to-cell communication, cell mobility, stable enzymes and RNAs, could allow AOB to maintain themselves under unfavourable circumstances. This review examines whether AOB possess such traits and how these traits might offer advantages over competing organ ...
1 Hernias 1.1 A few statistics 1.2 A look at the anatomy
... parts of the intestines, protrudes.s are a widespread phenomenon. Accordingly, around 27 % of men and 3% of women will suffer from an inguinal herniaThe most common type of hernia with hernial orifice above the inguinal ligament in the region of the lateral or medial inguinal fossa (Latin term: [no- ...
... parts of the intestines, protrudes.s are a widespread phenomenon. Accordingly, around 27 % of men and 3% of women will suffer from an inguinal herniaThe most common type of hernia with hernial orifice above the inguinal ligament in the region of the lateral or medial inguinal fossa (Latin term: [no- ...
The Cultural, Spiritual and Ethical Aspects of Xenotransplantation
... The body’s immune response is serious enough with donated human organs and tissues, but it is so severe with animal organs and tissues that xenotransplantation has not been seen as a viable option. However, interest in xenotransplantation has been rekindled as a result of recent advances in the gene ...
... The body’s immune response is serious enough with donated human organs and tissues, but it is so severe with animal organs and tissues that xenotransplantation has not been seen as a viable option. However, interest in xenotransplantation has been rekindled as a result of recent advances in the gene ...
Spatial Frequency Analysis in the Visual System
... of howto describe the receptive field of a retinal ganglion cell. Weuse this as an example, and then go on to discuss other major insights that have been provided by this approach. It is often useful to knowthe distribution of sensitivity within the receptive field: Wemaywant to knowhowthe propertie ...
... of howto describe the receptive field of a retinal ganglion cell. Weuse this as an example, and then go on to discuss other major insights that have been provided by this approach. It is often useful to knowthe distribution of sensitivity within the receptive field: Wemaywant to knowhowthe propertie ...
DEFINING THE ROLE OF THE SHP2 PROTEIN TYROSINE
... system3. They are involved in the inflammatory response through the combined activities of a variety of cell surface receptors, including the Toll Like Receptor (TLR) family and the high affinity IgE receptor, FcεRI3. The activities of the receptors expressed by mast cells allows them to be involved ...
... system3. They are involved in the inflammatory response through the combined activities of a variety of cell surface receptors, including the Toll Like Receptor (TLR) family and the high affinity IgE receptor, FcεRI3. The activities of the receptors expressed by mast cells allows them to be involved ...
Mucosal IL-10 and TGF-β play crucial roles in preventing LPS
... Most of our understanding of the effects of IL-10 in humans comes from the systemic administration of IL-10 to patients with Crohn disease (8–11) or to healthy volunteers subjected to experimental endotoxemia (14). In these conditions, IL-10 can induce a proinflammatory effect with enhanced Th1 immu ...
... Most of our understanding of the effects of IL-10 in humans comes from the systemic administration of IL-10 to patients with Crohn disease (8–11) or to healthy volunteers subjected to experimental endotoxemia (14). In these conditions, IL-10 can induce a proinflammatory effect with enhanced Th1 immu ...
Guanylate cyclase in Dictyostelium discoideum with the topology of
... The eukaryotic amoeba Dictyostelium discoideum grows as a unicellular organism in the presence of a food source, such as bacteria. Starvation induces a developmental programme leading to cell aggregation and the formation of a fruiting body, which is composed of a stalk with a spore head. Chemotaxis ...
... The eukaryotic amoeba Dictyostelium discoideum grows as a unicellular organism in the presence of a food source, such as bacteria. Starvation induces a developmental programme leading to cell aggregation and the formation of a fruiting body, which is composed of a stalk with a spore head. Chemotaxis ...
care of the wound bed assessment and management algorithm
... The removal of necrotic tissue and foreign material by the process of debridement is one of the most important factors in the management of chronic wounds. ...
... The removal of necrotic tissue and foreign material by the process of debridement is one of the most important factors in the management of chronic wounds. ...
EMD Millipore Protease and Phosphatase Inhibitor Cocktails
... cells and a finely tuned balance exists between their rate of synthesis and breakdown that determines the concentration of any given protein. Protein degradation is an essential process whereby damaged, unwanted, or “used” proteins are continuously eliminated. The extraordinary complexities of prote ...
... cells and a finely tuned balance exists between their rate of synthesis and breakdown that determines the concentration of any given protein. Protein degradation is an essential process whereby damaged, unwanted, or “used” proteins are continuously eliminated. The extraordinary complexities of prote ...
What`s the Difference? Efficient Set Reconciliation without
... sets stored at two nodes across a communication link. The set difference is the set of keys that are in one set but not the other. In reconciliation, the difference is used to compute the set union; in deduplication, it is used to compute the intersection. Efficiency is measured primarily by the ban ...
... sets stored at two nodes across a communication link. The set difference is the set of keys that are in one set but not the other. In reconciliation, the difference is used to compute the set union; in deduplication, it is used to compute the intersection. Efficiency is measured primarily by the ban ...
Cornell University, 1989 M.S. Environmental Engineering Stanford University, 1990
... Olson for their patience and devotion to my work over these many years. Through them, I have been able to gain many different insights into the world of oceanography, and science in general. Over my final year, I have benefited tremendously from involvements with two other scientists. Dave Karl open ...
... Olson for their patience and devotion to my work over these many years. Through them, I have been able to gain many different insights into the world of oceanography, and science in general. Over my final year, I have benefited tremendously from involvements with two other scientists. Dave Karl open ...
articles - Saleh Lab
... pharmacological evidence suggests that dsRNA entry is mediated by pattern-recognition receptors. The possible role of these receptors in dsRNA entry may link RNA interference (RNAi) silencing to other innate immune responses. RNAi is a highly conserved dsRNA-guided mechanism that mediates sequence-s ...
... pharmacological evidence suggests that dsRNA entry is mediated by pattern-recognition receptors. The possible role of these receptors in dsRNA entry may link RNA interference (RNAi) silencing to other innate immune responses. RNAi is a highly conserved dsRNA-guided mechanism that mediates sequence-s ...
PDF
... In the grafts treated with collagenase, hyaluronidase and RNase independently, the incidence of cartilage is around 35 %, compared with 52 % in control grafts. This depression of chondrogenesis may, however, be due to the fact that the agar-impregnated Millipore is not adhesive towards the tissues, ...
... In the grafts treated with collagenase, hyaluronidase and RNase independently, the incidence of cartilage is around 35 %, compared with 52 % in control grafts. This depression of chondrogenesis may, however, be due to the fact that the agar-impregnated Millipore is not adhesive towards the tissues, ...
An Important Pool of Sucrose Linked to Starch Biosynthesis is Taken
... ADPglucose. No changes in maximum catalytic activities of enzymes closely linked to starch and sucrose metabolism occurred in cells cultured with endocytic inhibitors. In addition, starch accumulation was unaffected by endocytic inhibitors when cells were cultured with glucose. These results provide ...
... ADPglucose. No changes in maximum catalytic activities of enzymes closely linked to starch and sucrose metabolism occurred in cells cultured with endocytic inhibitors. In addition, starch accumulation was unaffected by endocytic inhibitors when cells were cultured with glucose. These results provide ...
7 - Dynamic Microtubules and the Texture of Plant Cell Walls
... described as helicoidal. Crab shells, which are similarly based on chitin, are also said to have such a rotating arrangement of layers (Neville et al., 1976). Human bone, which is based on the fibrous protein, type 1 collagen, and a crystalline form of a calcium salt, has also been interpreted as ha ...
... described as helicoidal. Crab shells, which are similarly based on chitin, are also said to have such a rotating arrangement of layers (Neville et al., 1976). Human bone, which is based on the fibrous protein, type 1 collagen, and a crystalline form of a calcium salt, has also been interpreted as ha ...
WHO Guidelines on Tissue Infectivity Distribution in Transmissible
... might currently be incubating the disease would also be potential sources of infection for recipients. The possible extent of future blood-borne spread of vCJD infections is still unknown. The identification of these cases has intensified the concern about possible unmapped ways in which the disease ...
... might currently be incubating the disease would also be potential sources of infection for recipients. The possible extent of future blood-borne spread of vCJD infections is still unknown. The identification of these cases has intensified the concern about possible unmapped ways in which the disease ...
Tissue engineering
Tissue engineering is the use of a combination of cells, engineering and materials methods, and suitable biochemical and physicochemical factors to improve or replace biological functions. While it was once categorized as a sub-field of biomaterials, having grown in scope and importance it can be considered as a field in its own right.While most definitions of tissue engineering cover a broad range of applications, in practice the term is closely associated with applications that repair or replace portions of or whole tissues (i.e., bone, cartilage, blood vessels, bladder, skin, muscle etc.). Often, the tissues involved require certain mechanical and structural properties for proper functioning. The term has also been applied to efforts to perform specific biochemical functions using cells within an artificially-created support system (e.g. an artificial pancreas, or a bio artificial liver). The term regenerative medicine is often used synonymously with tissue engineering, although those involved in regenerative medicine place more emphasis on the use of stem cells or progenitor cells to produce tissues.