From segment to somite: Segmentation to
... Fig. 2. The model mechanisms underlying somite segmentation. A: An illustration of the anteroposterior (AP) axis and the various stages of somite formation according to the clock and wavefront model. The posterior presomitic mesoderm (PSM; shown in light gray) is homogeneous and cells are undetermin ...
... Fig. 2. The model mechanisms underlying somite segmentation. A: An illustration of the anteroposterior (AP) axis and the various stages of somite formation according to the clock and wavefront model. The posterior presomitic mesoderm (PSM; shown in light gray) is homogeneous and cells are undetermin ...
Thermal Diffusion as a Mechanism for Biological
... tween (a) the rate of heat production within the cell and (b) the rate of heat removal from the outer surface of the cell by conduction only. An estimate for the rate of heat production can be m ade from data on the bacterial cells Escherichia coli [36], Production of protein constitutes about 90% o ...
... tween (a) the rate of heat production within the cell and (b) the rate of heat removal from the outer surface of the cell by conduction only. An estimate for the rate of heat production can be m ade from data on the bacterial cells Escherichia coli [36], Production of protein constitutes about 90% o ...
IGF-1 induces rat glomerular mesangial cells to accumulate
... LDL (AcLDL) binding and degradation; and levels of protein expression of sterol-regulatory element binding protein-1c (SREBP-1c), the peroxisome proliferator-activated receptors (PPAR), PPAR␣, PPAR␥ and PPAR␦, scavenger receptor (SR) SR-B1, CD36, ATP-binding cassette A-1 (ABCA-1), and 3-hydroxy-3-me ...
... LDL (AcLDL) binding and degradation; and levels of protein expression of sterol-regulatory element binding protein-1c (SREBP-1c), the peroxisome proliferator-activated receptors (PPAR), PPAR␣, PPAR␥ and PPAR␦, scavenger receptor (SR) SR-B1, CD36, ATP-binding cassette A-1 (ABCA-1), and 3-hydroxy-3-me ...
THEME: HORMONAL DRUGS
... glands. For example, failure of the pancreatic islet cells leads to the development of diabetes mellitus, parathyroid gland failure which causes hypocalcaemia (associated with convulsions) and insufficiency of the antidiuretic hormone of the posterior pituitary lobe – diabetes insipidus. At the same ...
... glands. For example, failure of the pancreatic islet cells leads to the development of diabetes mellitus, parathyroid gland failure which causes hypocalcaemia (associated with convulsions) and insufficiency of the antidiuretic hormone of the posterior pituitary lobe – diabetes insipidus. At the same ...
Implications for AIDS Simian Immunodeficiency Virus Infection of
... In contrast to HIV-infected humans, naturally SIV-infected sooty mangabeys (SMs) very rarely progress to AIDS. Although the mechanisms underlying this disease resistance are unknown, a consistent feature of natural SIV infection is the absence of the generalized immune activation associated with HIV ...
... In contrast to HIV-infected humans, naturally SIV-infected sooty mangabeys (SMs) very rarely progress to AIDS. Although the mechanisms underlying this disease resistance are unknown, a consistent feature of natural SIV infection is the absence of the generalized immune activation associated with HIV ...
K-ras modulates the cell cycle via both positive and negative
... that have been maintained in RPMI 1640 medium containing or lacking Tet (1 mg/ml) were plated in a series of 96-well plates, which contain media supplemented with either 10% (at 2000 cells per well) or 0.1% FBS (at 5000 cells per well), with or without Tet added, respectively. The cells were incubat ...
... that have been maintained in RPMI 1640 medium containing or lacking Tet (1 mg/ml) were plated in a series of 96-well plates, which contain media supplemented with either 10% (at 2000 cells per well) or 0.1% FBS (at 5000 cells per well), with or without Tet added, respectively. The cells were incubat ...
Genome-wide gene expression in uro mutant
... and developmental processes and the uro mutant demonstrated several auxin defective phenotypes (Guo et al. 2004), it is possible that the altered expression levels of other hormone related genes may be caused by an indirect effect. Our data also revealed the altered expression levels of cell cycle a ...
... and developmental processes and the uro mutant demonstrated several auxin defective phenotypes (Guo et al. 2004), it is possible that the altered expression levels of other hormone related genes may be caused by an indirect effect. Our data also revealed the altered expression levels of cell cycle a ...
Muramatsu M
... Plasmids from 115 positive colonies that showed stronger signals with the cDNA probe derived from stimulated cells were sequenced, and their inserts were subjected to Northern blot hybridization to demonstrate up-regulated expression upon CSR in CH12F3-2 cells. Twentythree cDNA fragments were found ...
... Plasmids from 115 positive colonies that showed stronger signals with the cDNA probe derived from stimulated cells were sequenced, and their inserts were subjected to Northern blot hybridization to demonstrate up-regulated expression upon CSR in CH12F3-2 cells. Twentythree cDNA fragments were found ...
Understanding Lignification: Challenges Beyond
... from the cytoplasm to the cell wall. The translocation of small molecules across the cell membrane may occur by at least three different mechanisms: exocytosis, transporter-mediated export, and diffusion. Golgiderived vesicles are known to be involved in exporting some other cell wall components suc ...
... from the cytoplasm to the cell wall. The translocation of small molecules across the cell membrane may occur by at least three different mechanisms: exocytosis, transporter-mediated export, and diffusion. Golgiderived vesicles are known to be involved in exporting some other cell wall components suc ...
Derivation of naïve human embryonic stem cells
... state. Among them is ease of trypsin passage and developmental capacity. Whole animals can be generated from good naïve mESCs through tetraploid complementation (4), and mEpiSCs cannot contribute to chimerism. Being more comparable to mESCs, naïve hESCs will likely allow increased developmental pote ...
... state. Among them is ease of trypsin passage and developmental capacity. Whole animals can be generated from good naïve mESCs through tetraploid complementation (4), and mEpiSCs cannot contribute to chimerism. Being more comparable to mESCs, naïve hESCs will likely allow increased developmental pote ...
Evidence for inducible recruitment of Wiskott
... A) Nck1 and Nck2 are constitutively associated with WASp. Jurkat cells were unstimulated or stimulated for 5 min with 1 μg/ml anti-TCR antibody (C305). Immunoprecipitation (IP) was done with anti-WASp antibody followed by immunoblotted (IB) with corresponding antibodies. The membrane was reprobed wi ...
... A) Nck1 and Nck2 are constitutively associated with WASp. Jurkat cells were unstimulated or stimulated for 5 min with 1 μg/ml anti-TCR antibody (C305). Immunoprecipitation (IP) was done with anti-WASp antibody followed by immunoblotted (IB) with corresponding antibodies. The membrane was reprobed wi ...
Cells Last modified January 9, 2017 at 5:21 am
... Cells Question # 1 Chemical and structural bridges link groups or layers of similar cells, uniting them in structure and function as ...
... Cells Question # 1 Chemical and structural bridges link groups or layers of similar cells, uniting them in structure and function as ...
Association of Calmodulin and an Unconventional Myosin with the
... incubated 1.5 h at room temperature. The resin was washed three times in buffer I, then mixed with 300-700/~l of sucrose gradient fraction 7 (see Fig. 5). Additions were made to obtain the following final concentrations in the incubation mixture: 0.1% BSA, 0.005% Tween-20,, l0 ng/ml leupeptin, 10 ng ...
... incubated 1.5 h at room temperature. The resin was washed three times in buffer I, then mixed with 300-700/~l of sucrose gradient fraction 7 (see Fig. 5). Additions were made to obtain the following final concentrations in the incubation mixture: 0.1% BSA, 0.005% Tween-20,, l0 ng/ml leupeptin, 10 ng ...
TOPIC 5 Energy for biological processes 5.1 Cellular respiration
... mitochondrial membranes, using the energy provided as the electrons pass along the transport chain. The inner mitochondrial membrane is impermeable to protons. This means that as a result of the active transport of the protons there are different hydrogen ion concentrations on the two sides of the i ...
... mitochondrial membranes, using the energy provided as the electrons pass along the transport chain. The inner mitochondrial membrane is impermeable to protons. This means that as a result of the active transport of the protons there are different hydrogen ion concentrations on the two sides of the i ...
PLANT CELL WALL PROTEINS
... It took 85 million years for land plants to evolve from their green-algae ancestors. One of the most notable changes that occurred was the development of different kinds of cell walls. The vast ecological diversity of plants is directly associated with a great variety in size, shape, form, and funct ...
... It took 85 million years for land plants to evolve from their green-algae ancestors. One of the most notable changes that occurred was the development of different kinds of cell walls. The vast ecological diversity of plants is directly associated with a great variety in size, shape, form, and funct ...
Hoeflea phototrophica sp. nov., a novel marine aerobic
... or no growth at all. Growth factors were required; these were provided by small amounts of yeast extract, but not by standard vitamin solutions. Growth occurred under aerobic to microaerobic conditions, but not under anaerobic conditions, either in the dark or light. Nitrate was not reduced. Photosy ...
... or no growth at all. Growth factors were required; these were provided by small amounts of yeast extract, but not by standard vitamin solutions. Growth occurred under aerobic to microaerobic conditions, but not under anaerobic conditions, either in the dark or light. Nitrate was not reduced. Photosy ...
Chapter 6 Cell - SCF Faculty Site Homepage
... 1. All organisms are composed of one or more cells ...
... 1. All organisms are composed of one or more cells ...
pig-1_final 121812
... and the generation of daughter cells with distinct fates. Changes in cell fate may underlie many of the abnormal behaviors exhibited by cells after loss of PAR-4 or LKB1. ...
... and the generation of daughter cells with distinct fates. Changes in cell fate may underlie many of the abnormal behaviors exhibited by cells after loss of PAR-4 or LKB1. ...
Systematic identification of cell size regulators in
... The emerging molecular model of size control in budding yeast is centered on the SBF/MBF transcription complex, whose activity is inhibited by the transcription repressor Whi5 that binds SBF in late M/early G1 (Jorgensen et al, 2002; Costanzo et al, 2004; De Bruin et al, 2004). It is suggested that ...
... The emerging molecular model of size control in budding yeast is centered on the SBF/MBF transcription complex, whose activity is inhibited by the transcription repressor Whi5 that binds SBF in late M/early G1 (Jorgensen et al, 2002; Costanzo et al, 2004; De Bruin et al, 2004). It is suggested that ...
(Human Umbilical Vein Endothelial Cells
... Products > HUVEC Transfection Reagent (Human Umbilical Vein Endothelial Cells) Altogen Biosystems offers the HUVEC Transfection Reagent among a host of 100+ cell line specific In Vitro Transfection Kits. The HUVEC Transfection Reagent is an advanced formulation of a lipid based reagent, and it has b ...
... Products > HUVEC Transfection Reagent (Human Umbilical Vein Endothelial Cells) Altogen Biosystems offers the HUVEC Transfection Reagent among a host of 100+ cell line specific In Vitro Transfection Kits. The HUVEC Transfection Reagent is an advanced formulation of a lipid based reagent, and it has b ...
BCH364C-391L_SyntheticBio1_Spring2015
... Synthetic Biology = design and engineering of biological systems that aren’t found in nature Why would we want to do this? - Want to understand natural systems. One of the best ways to understand a system is to change it or make new, related ones - To fully “understand” a system, we should be able t ...
... Synthetic Biology = design and engineering of biological systems that aren’t found in nature Why would we want to do this? - Want to understand natural systems. One of the best ways to understand a system is to change it or make new, related ones - To fully “understand” a system, we should be able t ...
The putative phosphatase All1758 is necessary for normal growth
... cell division genes ftsX and ftsY may be indicative of a role for all1758 in cell division. Taken together, these results suggest that the protein encoded by all1758 may represent a link between cell growth, division and regulation of the morphological differentiation of heterocysts. ...
... cell division genes ftsX and ftsY may be indicative of a role for all1758 in cell division. Taken together, these results suggest that the protein encoded by all1758 may represent a link between cell growth, division and regulation of the morphological differentiation of heterocysts. ...
Nucleolar targeting of BN46/51 - Journal of Cell Science
... with the mAbs BN5.1, AH6 and DE6 (Trimbur and Walsh, 1992) followed by horseradish peroxidase-coupled second antibody and visualized with either 4-chloro-1-naphthol and H2O2 (Trimbur and Walsh, 1992) or using the ECL method (Amersham). For the isolation of nuclei, M15 cells grown to confluence in 25 ...
... with the mAbs BN5.1, AH6 and DE6 (Trimbur and Walsh, 1992) followed by horseradish peroxidase-coupled second antibody and visualized with either 4-chloro-1-naphthol and H2O2 (Trimbur and Walsh, 1992) or using the ECL method (Amersham). For the isolation of nuclei, M15 cells grown to confluence in 25 ...
Using glyco-engineering to produce therapeutic proteins
... and function of the different OST subunits. Mammalian cells contain two OST complexes that differ in their catalytic subunit as well as in accessory proteins. These OST complexes display partially overlapping functions, but also preferences for certain glycoprotein substrates [3]. Engineering of the ...
... and function of the different OST subunits. Mammalian cells contain two OST complexes that differ in their catalytic subunit as well as in accessory proteins. These OST complexes display partially overlapping functions, but also preferences for certain glycoprotein substrates [3]. Engineering of the ...
Cell culture
Cell culture is the process by which cells are grown under controlled conditions, generally outside of their natural environment. In practice, the term ""cell culture"" now refers to the culturing of cells derived from multicellular eukaryotes, especially animal cells, in contrast with other types of culture that also grow cells, such as plant tissue culture, fungal culture, and microbiological culture (of microbes). The historical development and methods of cell culture are closely interrelated to those of tissue culture and organ culture. Viral culture is also related, with cells as hosts for the viruses. The laboratory technique of maintaining live cell lines (a population of cells descended from a single cell and containing the same genetic makeup) separated from their original tissue source became more robust in the middle 20th century.