Dynamic targeting of the replication machinery to sites of DNA damage
... the repair of genetic lesions (Rouse and Jackson, 2002). Apart from its function in the replication of the genome, the DNA replication machinery plays an essential role in the repair of damaged DNA. The heterotrimeric replication protein A (RPA) complex has been implicated in the damage detection pr ...
... the repair of genetic lesions (Rouse and Jackson, 2002). Apart from its function in the replication of the genome, the DNA replication machinery plays an essential role in the repair of damaged DNA. The heterotrimeric replication protein A (RPA) complex has been implicated in the damage detection pr ...
A Role for Eosinophils in Adaptive Humoral Immunity
... interleukins, chemokines and RNAses (collectively known as eokines), which can be rapidly released during an inflammatory response to foreign proteins. Their accumulation and release of diverse eokines during the inflammation that accompanies an anamnestic response suggests an immunoregulatory funct ...
... interleukins, chemokines and RNAses (collectively known as eokines), which can be rapidly released during an inflammatory response to foreign proteins. Their accumulation and release of diverse eokines during the inflammation that accompanies an anamnestic response suggests an immunoregulatory funct ...
Cell Division Activity during Apical Hook
... understand how cell division contributes to differential growth, four different parts of the hook were defined (apical [a], basal [b], inner [i], and outer [o]; Figure 2A), and the number of subepidermal cells in each part was counted. The major difference in cell number was found between apical and ...
... understand how cell division contributes to differential growth, four different parts of the hook were defined (apical [a], basal [b], inner [i], and outer [o]; Figure 2A), and the number of subepidermal cells in each part was counted. The major difference in cell number was found between apical and ...
Skeletal Muscle Stem Cells in the Spotlight: The Satellite Cell
... mainly with mouse tissue) have established that satellite cells can be isolated on the basis of being negative for CD45, CD31, and Sca1, and positive for CD34 and a7 integrin [57, 58]. Additional cell surface antigens, including CXCR4, b1 integrin, and syndecan-4 have also been utilized for isolatio ...
... mainly with mouse tissue) have established that satellite cells can be isolated on the basis of being negative for CD45, CD31, and Sca1, and positive for CD34 and a7 integrin [57, 58]. Additional cell surface antigens, including CXCR4, b1 integrin, and syndecan-4 have also been utilized for isolatio ...
Role of - Molecular Physiology and Biophysics
... domain of the a2 chain of laminin-2. Native a-DG competitively inhibited the laminin-2–mediated M. leprae binding to primary Schwann cells. Thus, M. leprae may use linkage between the extracellular matrix and cytoskeleton through laminin-2 and a-DG for its interaction with Schwann cells. Pathogenic ...
... domain of the a2 chain of laminin-2. Native a-DG competitively inhibited the laminin-2–mediated M. leprae binding to primary Schwann cells. Thus, M. leprae may use linkage between the extracellular matrix and cytoskeleton through laminin-2 and a-DG for its interaction with Schwann cells. Pathogenic ...
Leaf growth in grasses is determined by the cell division and
... regulation such as expansin of cell wall extensibility as a key process in controlling growth in plants and have led to the identification of several proteins that are potentially involved in this process (Cosgrove 1999). For instance, the XET-related gene, FpXET1, is a potential marker for leaf elo ...
... regulation such as expansin of cell wall extensibility as a key process in controlling growth in plants and have led to the identification of several proteins that are potentially involved in this process (Cosgrove 1999). For instance, the XET-related gene, FpXET1, is a potential marker for leaf elo ...
Title in Title Case and Bolded
... Cell culture－Human osteoblast-like cell line G-292 (ATCC CRL 1423) , originally isolated from a human osteosarcoma. Cells were initially cultured in McCoy’5a medium containing 10﹪fetal bovine serum (FBS), 100 units/ml of penicillin and 100 μg/ml of strepotmycin. The cells were then thawed and cultur ...
... Cell culture－Human osteoblast-like cell line G-292 (ATCC CRL 1423) , originally isolated from a human osteosarcoma. Cells were initially cultured in McCoy’5a medium containing 10﹪fetal bovine serum (FBS), 100 units/ml of penicillin and 100 μg/ml of strepotmycin. The cells were then thawed and cultur ...
D:Int Agrophysics -2Cybulskacybulska.vp - Research
... arranged mainly randomly but there are also some bundles of several microfibrils. Similar bundles were observed in AFM images of chestnut cell walls (Kirby et al., 1996). Their presence is probably an evidence of crystalline structure of cellulose microfibrils. After adding pectin to culture medium ...
... arranged mainly randomly but there are also some bundles of several microfibrils. Similar bundles were observed in AFM images of chestnut cell walls (Kirby et al., 1996). Their presence is probably an evidence of crystalline structure of cellulose microfibrils. After adding pectin to culture medium ...
Commentary in EMBO Journal
... waste items from which useful materials can be recovered and reused. Cells engage in analogous activities, and autophagy is an integral part of the larger waste recycling strategy. Autophagy, the process of cellular “self-digestion”, was initially described by Christian de Duve, was outlined in mole ...
... waste items from which useful materials can be recovered and reused. Cells engage in analogous activities, and autophagy is an integral part of the larger waste recycling strategy. Autophagy, the process of cellular “self-digestion”, was initially described by Christian de Duve, was outlined in mole ...
Visualization of Intracellular Transport of Vesicular Stomatitis Virus
... are transported toward the cell periphery via a microtubule (MT)-mediated process, and the nucleocapsids were seen to be closely associated with mitochondria. Treatment of cells with nocodazole or Colcemid, drugs known to inhibit MT polymerization, resulted in accumulation of the nucleocapsids aroun ...
... are transported toward the cell periphery via a microtubule (MT)-mediated process, and the nucleocapsids were seen to be closely associated with mitochondria. Treatment of cells with nocodazole or Colcemid, drugs known to inhibit MT polymerization, resulted in accumulation of the nucleocapsids aroun ...
Primary endothelial cells isolated from the yolk sac
... expansion in vivo. At present it is unclear which cells of the yolk sac microenvironment stimulate the HSC expansion. Several groups have isolated yolk sac and AGM endothelial cell lines and reported that these cell lines stimulate HSC and progenitor cell proliferation and differentiation in vitro.1 ...
... expansion in vivo. At present it is unclear which cells of the yolk sac microenvironment stimulate the HSC expansion. Several groups have isolated yolk sac and AGM endothelial cell lines and reported that these cell lines stimulate HSC and progenitor cell proliferation and differentiation in vitro.1 ...
Protozoa as Model System for Studies of
... ciliates avoid lighted areas are not clear at present. It is known that prolonged cell exposure to intensive light levels in the visible range causes significant disturbances in the occurrence of the photophobic response in both ciliates and can even lead to their death as a result of the photodynam ...
... ciliates avoid lighted areas are not clear at present. It is known that prolonged cell exposure to intensive light levels in the visible range causes significant disturbances in the occurrence of the photophobic response in both ciliates and can even lead to their death as a result of the photodynam ...
Culture media - INAYA Medical College
... Are the building blocks of proteins. ‘Essential’ amino acids must be added to culture media as cells are not able to synthesize these themselves. Example: cystine, leucine, lysine, methionine, phenylalanine, tryptophan, and valine. 2. Carbohydrates The main source of energy is derived from s ...
... Are the building blocks of proteins. ‘Essential’ amino acids must be added to culture media as cells are not able to synthesize these themselves. Example: cystine, leucine, lysine, methionine, phenylalanine, tryptophan, and valine. 2. Carbohydrates The main source of energy is derived from s ...
Presentation by Human Dendritic Cells Killed, and Processed for
... Intracellular growth of Hc yeasts in DC and M was quantified by the incorporation of [3H]leucine as described previously (16). DC were incubated at varying ratios of cells to yeasts (50/1, 10/1, and 5/1) in polypropylene tubes with 5 ⫻ 103 viable Hc yeasts for 48 h at 37°C in a water bath with orbi ...
... Intracellular growth of Hc yeasts in DC and M was quantified by the incorporation of [3H]leucine as described previously (16). DC were incubated at varying ratios of cells to yeasts (50/1, 10/1, and 5/1) in polypropylene tubes with 5 ⫻ 103 viable Hc yeasts for 48 h at 37°C in a water bath with orbi ...
Life Science Leoce review
... What could cause a Scientific theory to be modified? •New scientific discoveries are often based on previous data or concepts •Theories are based on the best available evidence, but they may change as new evidence is discovered ...
... What could cause a Scientific theory to be modified? •New scientific discoveries are often based on previous data or concepts •Theories are based on the best available evidence, but they may change as new evidence is discovered ...
Nitric Oxide 9:
... or nitric oxide (NO) via oxidation or nitrosation, respectively, of an OxyR cysteine redox center [9,17–19]. H2 O2 and NO can also activate SoxR, another transcriptional global regulator of genes involved in oxidative stress tolerance, such as sodA, which encodes a manganese SOD [9,20]. NO-related a ...
... or nitric oxide (NO) via oxidation or nitrosation, respectively, of an OxyR cysteine redox center [9,17–19]. H2 O2 and NO can also activate SoxR, another transcriptional global regulator of genes involved in oxidative stress tolerance, such as sodA, which encodes a manganese SOD [9,20]. NO-related a ...
Tissues - MyCAERT
... Can withstand tension in many directions Irregularly arranged collagen fibers Major cell type is fibroblasts ...
... Can withstand tension in many directions Irregularly arranged collagen fibers Major cell type is fibroblasts ...
Nature Cell Biol. 6
... and membrane ruffles, which was also insensitive to pre-treatment with rapamycin (see Supplementary Information, Fig. S1). The addition of lysophosphatidic acid (LPA) also led to rapamycin-insensitive formation of stress fibres. Similar results were obtained with serum-, insulin- or LPA-treated Swis ...
... and membrane ruffles, which was also insensitive to pre-treatment with rapamycin (see Supplementary Information, Fig. S1). The addition of lysophosphatidic acid (LPA) also led to rapamycin-insensitive formation of stress fibres. Similar results were obtained with serum-, insulin- or LPA-treated Swis ...
Polypeptide Composition of Chlamydia trachomatis
... Infecfiuify tirration. Serial five-fold dilutions of egg-grown chlamydiae were prepared in Eagle's Minimal Essential Medium containing 10 o/o (v/v) foetal calf serum, L-glutamine and sodium bicarbonate (MEM). The chlamydial dilutions in 1 ml volumes of MEM were centrifuged at 2000g for 1 h at room t ...
... Infecfiuify tirration. Serial five-fold dilutions of egg-grown chlamydiae were prepared in Eagle's Minimal Essential Medium containing 10 o/o (v/v) foetal calf serum, L-glutamine and sodium bicarbonate (MEM). The chlamydial dilutions in 1 ml volumes of MEM were centrifuged at 2000g for 1 h at room t ...
SIM002 Sig Tech Liquid Kelp - hugh
... SIGNATURE LIQUID KELP is a concentrated form of natural plant hormones derived from seaweed. Extracted from the fastest growing seaweed on the planet (Ecklonia maxima), Signature Liquid Kelp delivers Cytokinins and Auxins to help expand and increase the root mass of turfgrass. Simply, without the pr ...
... SIGNATURE LIQUID KELP is a concentrated form of natural plant hormones derived from seaweed. Extracted from the fastest growing seaweed on the planet (Ecklonia maxima), Signature Liquid Kelp delivers Cytokinins and Auxins to help expand and increase the root mass of turfgrass. Simply, without the pr ...
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... applying daily heat-shock treatment (Kataoka et al., 2001). In this study, we distinguished GSCs from differentiated germ cells by using anti-Vasa and anti-Hts antibodies to visualize germ cells and fusomes, respectively. The fusome (also called a ‘spectrosome’ in GSCs/CBs) is a germ-cell-specific o ...
... applying daily heat-shock treatment (Kataoka et al., 2001). In this study, we distinguished GSCs from differentiated germ cells by using anti-Vasa and anti-Hts antibodies to visualize germ cells and fusomes, respectively. The fusome (also called a ‘spectrosome’ in GSCs/CBs) is a germ-cell-specific o ...
Murine gammaherpesvirus 68 open reading frame 35 is required for
... viral DNA polymerase inhibitor, no genome replication was observed, as expected. All of ...
... viral DNA polymerase inhibitor, no genome replication was observed, as expected. All of ...
Lecture_8
... and motility. Two subunits of this protein complex are actin-related proteins (ARPs) belonging to the Arp2 and Arp3 subfamilies. The Arp3 subunit localizes to the surface of stationary bacteria and the tails of motile bacteria in tissue culture cells infected with L. monocytogenes; this is consisten ...
... and motility. Two subunits of this protein complex are actin-related proteins (ARPs) belonging to the Arp2 and Arp3 subfamilies. The Arp3 subunit localizes to the surface of stationary bacteria and the tails of motile bacteria in tissue culture cells infected with L. monocytogenes; this is consisten ...
research article Numerical study of the electroporation pulse shape
... world and for this reason it has drawn the attention of many researchers. Chemotherapy has been used for many years and is one of the most common treatments for cancer. Cytotoxic chemotherapeutic drugs are usually hydrophilic with very low transport through the cell membrane and thus high doses of t ...
... world and for this reason it has drawn the attention of many researchers. Chemotherapy has been used for many years and is one of the most common treatments for cancer. Cytotoxic chemotherapeutic drugs are usually hydrophilic with very low transport through the cell membrane and thus high doses of t ...
Cell culture
Cell culture is the process by which cells are grown under controlled conditions, generally outside of their natural environment. In practice, the term ""cell culture"" now refers to the culturing of cells derived from multicellular eukaryotes, especially animal cells, in contrast with other types of culture that also grow cells, such as plant tissue culture, fungal culture, and microbiological culture (of microbes). The historical development and methods of cell culture are closely interrelated to those of tissue culture and organ culture. Viral culture is also related, with cells as hosts for the viruses. The laboratory technique of maintaining live cell lines (a population of cells descended from a single cell and containing the same genetic makeup) separated from their original tissue source became more robust in the middle 20th century.