![The putative phosphatase All1758 is necessary for normal growth](http://s1.studyres.com/store/data/007200482_1-05c174fdf196a93f5e4ddd425d593c15-300x300.png)
The putative phosphatase All1758 is necessary for normal growth
... cell division genes ftsX and ftsY may be indicative of a role for all1758 in cell division. Taken together, these results suggest that the protein encoded by all1758 may represent a link between cell growth, division and regulation of the morphological differentiation of heterocysts. ...
... cell division genes ftsX and ftsY may be indicative of a role for all1758 in cell division. Taken together, these results suggest that the protein encoded by all1758 may represent a link between cell growth, division and regulation of the morphological differentiation of heterocysts. ...
Practical Hints: Lysis of bacterial cells for plasmid purification
... Specialized modules are available for different scales of plasmid preparation, as well ...
... Specialized modules are available for different scales of plasmid preparation, as well ...
Using glyco-engineering to produce therapeutic proteins
... and function of the different OST subunits. Mammalian cells contain two OST complexes that differ in their catalytic subunit as well as in accessory proteins. These OST complexes display partially overlapping functions, but also preferences for certain glycoprotein substrates [3]. Engineering of the ...
... and function of the different OST subunits. Mammalian cells contain two OST complexes that differ in their catalytic subunit as well as in accessory proteins. These OST complexes display partially overlapping functions, but also preferences for certain glycoprotein substrates [3]. Engineering of the ...
Glucose Metabolism in T Cells and Monocytes: New Perspectives in
... 2012). Indeed, Macintyre and colleagues have revealed that Glut1 is essential for metabolic programming of CD4+ T cell activation, expansion and survival (Macintyre et al., 2014). Glycolysis results in the production of pyruvate from glucose with only a net of two adenosine triphosphates (ATPs) per ...
... 2012). Indeed, Macintyre and colleagues have revealed that Glut1 is essential for metabolic programming of CD4+ T cell activation, expansion and survival (Macintyre et al., 2014). Glycolysis results in the production of pyruvate from glucose with only a net of two adenosine triphosphates (ATPs) per ...
Multiscale Systems Analysis of Root Growth and
... between cells are treated. Cellular Potts (or Glazier-GranerHogeweg) models represent cells as collections of voxels (volumetric elements, typically cuboids) that undergo growth through stochastic relabeling rules (Graner and Glazier, 1992; Glazier and Graner, 1993). Frameworks of this type were ini ...
... between cells are treated. Cellular Potts (or Glazier-GranerHogeweg) models represent cells as collections of voxels (volumetric elements, typically cuboids) that undergo growth through stochastic relabeling rules (Graner and Glazier, 1992; Glazier and Graner, 1993). Frameworks of this type were ini ...
Differences between Edexcel `A` and `B`
... Understand the similarities and differences between the structures and functions of haemoglobin and myoglobin. Understand the significance of the oxygen affinity of fetal haemoglobin as compared to adult haemoglobin. Understand how the interchange of substances occurs through the formation and reabs ...
... Understand the similarities and differences between the structures and functions of haemoglobin and myoglobin. Understand the significance of the oxygen affinity of fetal haemoglobin as compared to adult haemoglobin. Understand how the interchange of substances occurs through the formation and reabs ...
Stamen Structure and Function
... and model species, including tomato (Ursin et al., 1989; Chmelnitsky et al., 2003), tobacco (Koltunow et al., 1990), Antirrhinum (Nacken et al., 1991), oilseed rape (Scott et al., 1991b; Shen and Hsu, 1992), maize (Wright et al., 1993), rice (Tsuchiya et al., 1994), lily (Crossley et al., 1995), whi ...
... and model species, including tomato (Ursin et al., 1989; Chmelnitsky et al., 2003), tobacco (Koltunow et al., 1990), Antirrhinum (Nacken et al., 1991), oilseed rape (Scott et al., 1991b; Shen and Hsu, 1992), maize (Wright et al., 1993), rice (Tsuchiya et al., 1994), lily (Crossley et al., 1995), whi ...
"Unusual" modifications and variations of
... further minor variations and modifications that occur less frequently. Many examples presented above from our own work fall into this category and, for the most part, have not been related to any specific function. The same is true in a variety of novel oligosaccharide modifications that have not be ...
... further minor variations and modifications that occur less frequently. Many examples presented above from our own work fall into this category and, for the most part, have not been related to any specific function. The same is true in a variety of novel oligosaccharide modifications that have not be ...
Cytometry and Plant Sciences: a Personal Retrospective David W. Galbraith
... analyzed, and we extended the method to the analysis of haploids in tissue culture (14), of natural variation in cytotype distributions (15), and for addressing issues in angiosperm evolution (16). Flow cytometry also can be used for quality control monitoring of the ploidy of commercial seeds and o ...
... analyzed, and we extended the method to the analysis of haploids in tissue culture (14), of natural variation in cytotype distributions (15), and for addressing issues in angiosperm evolution (16). Flow cytometry also can be used for quality control monitoring of the ploidy of commercial seeds and o ...
Potassium regulation
... the potassium concentration inside the cell higher (the cell actually will shrink) the thing that will drive potassium outside the cell causing hyperkalemia • For each 10 mOsm increase in osmalrity, this will make 0.4-0.8 mEqv increase in potassium extracellular concentration • Remember that the nor ...
... the potassium concentration inside the cell higher (the cell actually will shrink) the thing that will drive potassium outside the cell causing hyperkalemia • For each 10 mOsm increase in osmalrity, this will make 0.4-0.8 mEqv increase in potassium extracellular concentration • Remember that the nor ...
Identification and characterization of subpopulations
... Identification and characterization of subpopulations in undifferentiated ES cell culture Yayoi Toyooka1,2,*, Daisuke Shimosato1,3, Kazuhiro Murakami1, Kadue Takahashi1 and Hitoshi Niwa1,3,* Embryonic stem (ES) cells are pluripotent cells derived from the inner cell mass (ICM) and the epiblast, and ...
... Identification and characterization of subpopulations in undifferentiated ES cell culture Yayoi Toyooka1,2,*, Daisuke Shimosato1,3, Kazuhiro Murakami1, Kadue Takahashi1 and Hitoshi Niwa1,3,* Embryonic stem (ES) cells are pluripotent cells derived from the inner cell mass (ICM) and the epiblast, and ...
IL-13Ra1 Expression on b-Cell–Specific T Cells in NOD
... described previously (9). The p524-reactive T-cell clone GAD35Za was isolated from peptide-immunized mice (9). Briefly, spleen and lymph node cells from NOD mice immunized with p524-538 (20 mg of peptide p524 in complete Freund’s adjuvant) were challenged in vitro with cognate peptide, and expanded w ...
... described previously (9). The p524-reactive T-cell clone GAD35Za was isolated from peptide-immunized mice (9). Briefly, spleen and lymph node cells from NOD mice immunized with p524-538 (20 mg of peptide p524 in complete Freund’s adjuvant) were challenged in vitro with cognate peptide, and expanded w ...
Aluminum-Induced 133-ß-D-Glucan Inhibits Cell
... Symplastic intercellular transport in plants is achieved by plasmodesmata (PD). These cytoplasmic channels are well known to interconnect plant cells to facilitate intercellular movement of water, nutrients, and signaling molecules including hormones. However, it is not known whether Al may affect t ...
... Symplastic intercellular transport in plants is achieved by plasmodesmata (PD). These cytoplasmic channels are well known to interconnect plant cells to facilitate intercellular movement of water, nutrients, and signaling molecules including hormones. However, it is not known whether Al may affect t ...
Applied and Environmental Microbiology
... aqueous environment. Rosettes in aqueous suspensions were interpreted by them as micelles with the hydrophobic ends of the cells attracted to each other. Polar orientation could not be explained by localization of surface ionogenic groups (10), and both infective and noninfective R. trifolii strains ...
... aqueous environment. Rosettes in aqueous suspensions were interpreted by them as micelles with the hydrophobic ends of the cells attracted to each other. Polar orientation could not be explained by localization of surface ionogenic groups (10), and both infective and noninfective R. trifolii strains ...
Altered Cell Fate in LiCI-Treated Sea Urchin Embryos
... mM), staining was observed in small groups of cells within the larger masses present, but again, no obvious organization was evident (data not shown). To quantitate the increase in endoderm tissue suggested by the photographs presented in Fig. 2, we dissociated control and lithium-treated embryos in ...
... mM), staining was observed in small groups of cells within the larger masses present, but again, no obvious organization was evident (data not shown). To quantitate the increase in endoderm tissue suggested by the photographs presented in Fig. 2, we dissociated control and lithium-treated embryos in ...
The Golgi Apparatus
... about in the plane of the membrane • This behavior leads to the description of a membrane as a fluid mosaic – Molecules can move freely within the membrane – A diversity of proteins exists within the membrane ...
... about in the plane of the membrane • This behavior leads to the description of a membrane as a fluid mosaic – Molecules can move freely within the membrane – A diversity of proteins exists within the membrane ...
mitosis on DNA replication and repair. Mitotic checkpoint genes in
... In eukaryotes a cell-cycle control termed a checkpoint causes arrest in the S or G2 phases when chromosomes are incompletely replicated or damaged. Previously, we showed in budding yeast that RAD9 and RAD17 are checkpoint genes required for arrest in the G2 phase after DNA damage. Here, we describe ...
... In eukaryotes a cell-cycle control termed a checkpoint causes arrest in the S or G2 phases when chromosomes are incompletely replicated or damaged. Previously, we showed in budding yeast that RAD9 and RAD17 are checkpoint genes required for arrest in the G2 phase after DNA damage. Here, we describe ...
Patterns of Collective Bacterial Motion in Microfluidic Devices
... trient availability or faster biochemical signal propagation due to enhanced diffusion). Interestingly, pattern formation has also been observed for suspensions of non-active agents.8 Settling of colloid particles under gravitation is an actively studied field of statistical mechanics, and computat ...
... trient availability or faster biochemical signal propagation due to enhanced diffusion). Interestingly, pattern formation has also been observed for suspensions of non-active agents.8 Settling of colloid particles under gravitation is an actively studied field of statistical mechanics, and computat ...
Cellulose orientation determines mechanical anisotropy in onion
... these tissues were determined. It was demonstrated for the first time for higher plants that a net parallel orientation of cellulose microfibrils in the onion epidermis wall coincided with a high mechanical anisotropy. In contrast, the Kalanchoë epidermis had a random net orientation of microfibril ...
... these tissues were determined. It was demonstrated for the first time for higher plants that a net parallel orientation of cellulose microfibrils in the onion epidermis wall coincided with a high mechanical anisotropy. In contrast, the Kalanchoë epidermis had a random net orientation of microfibril ...
Nuclear envelope dynamics during plant cell division suggest
... receptor) or LEM (Lap-Emerin-Man) domain proteins are absent in plants. This is also true for lamins. However, recent research of the dynamics of two lamin-like proteins, NMCP (nuclear matrix constituent protein) 1 and NMCP2, suggests that these also disassemble at the beginning of mitosis in Apium ...
... receptor) or LEM (Lap-Emerin-Man) domain proteins are absent in plants. This is also true for lamins. However, recent research of the dynamics of two lamin-like proteins, NMCP (nuclear matrix constituent protein) 1 and NMCP2, suggests that these also disassemble at the beginning of mitosis in Apium ...
Chapter 2 - William Stallings, Data and Computer
... propagation delay and switching delay • assume propagation at two-thirds speed of light • if source and destination on opposite sides of USA, propagation time ~ 48x10-3 seconds • given implicit congestion control, by the time dropped cell notification has reached source, 7.2x106 bits have been trans ...
... propagation delay and switching delay • assume propagation at two-thirds speed of light • if source and destination on opposite sides of USA, propagation time ~ 48x10-3 seconds • given implicit congestion control, by the time dropped cell notification has reached source, 7.2x106 bits have been trans ...
Stamen Structure and Function
... and model species, including tomato (Ursin et al., 1989; Chmelnitsky et al., 2003), tobacco (Koltunow et al., 1990), Antirrhinum (Nacken et al., 1991), oilseed rape (Scott et al., 1991b; Shen and Hsu, 1992), maize (Wright et al., 1993), rice (Tsuchiya et al., 1994), lily (Crossley et al., 1995), whi ...
... and model species, including tomato (Ursin et al., 1989; Chmelnitsky et al., 2003), tobacco (Koltunow et al., 1990), Antirrhinum (Nacken et al., 1991), oilseed rape (Scott et al., 1991b; Shen and Hsu, 1992), maize (Wright et al., 1993), rice (Tsuchiya et al., 1994), lily (Crossley et al., 1995), whi ...
Lactic acid excretion by Streptococcus mutans
... Lactic acid is the major end-product of glycolysis by Streptococcus mutans under conditions of sugar excess or low environmental pH. However, the mechanism of lactic acid excretion by S. mutans is unknown. To characterize lactic acid efflux in 5. mutans the transmembrane movement of radiolabelled la ...
... Lactic acid is the major end-product of glycolysis by Streptococcus mutans under conditions of sugar excess or low environmental pH. However, the mechanism of lactic acid excretion by S. mutans is unknown. To characterize lactic acid efflux in 5. mutans the transmembrane movement of radiolabelled la ...
Transfer of Primordial Germ-cells inXenopus laevis
... In the experiments, care was taken at the time of operation to graft pieces of endoderm very similar in size. In view of the argument at the end of the last paragraph, one is naturally inclined to ask why the graft is successful in some cases and not in others. We would draw attention to the signifi ...
... In the experiments, care was taken at the time of operation to graft pieces of endoderm very similar in size. In view of the argument at the end of the last paragraph, one is naturally inclined to ask why the graft is successful in some cases and not in others. We would draw attention to the signifi ...
Morphology and LPS content for the estimation of marine
... as a ground truth baseline on which a microbiological interpretation can be developed (Ducklow, 2000). The total cell count in combination with cell volume measurements is considered a key parameter that provides ecologically important information about the bacterial assemblage (Posch et al., 2001; ...
... as a ground truth baseline on which a microbiological interpretation can be developed (Ducklow, 2000). The total cell count in combination with cell volume measurements is considered a key parameter that provides ecologically important information about the bacterial assemblage (Posch et al., 2001; ...
Cell culture
![](https://commons.wikimedia.org/wiki/Special:FilePath/Cell_Culture_in_a_tiny_Petri_dish.jpg?width=300)
Cell culture is the process by which cells are grown under controlled conditions, generally outside of their natural environment. In practice, the term ""cell culture"" now refers to the culturing of cells derived from multicellular eukaryotes, especially animal cells, in contrast with other types of culture that also grow cells, such as plant tissue culture, fungal culture, and microbiological culture (of microbes). The historical development and methods of cell culture are closely interrelated to those of tissue culture and organ culture. Viral culture is also related, with cells as hosts for the viruses. The laboratory technique of maintaining live cell lines (a population of cells descended from a single cell and containing the same genetic makeup) separated from their original tissue source became more robust in the middle 20th century.