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here - The Nebenführ Lab
... In the course of a previous study on actomyosin-dependent Golgi stack movement (Nebenführ et al. 1999), we noticed that treatment of BY-2 cells with the myosin inhibitor BDM not only blocked Golgi stack movement but also seemed to affect the dynamics of TVS. In this study, we give a thorough descrip ...
... In the course of a previous study on actomyosin-dependent Golgi stack movement (Nebenführ et al. 1999), we noticed that treatment of BY-2 cells with the myosin inhibitor BDM not only blocked Golgi stack movement but also seemed to affect the dynamics of TVS. In this study, we give a thorough descrip ...
this PDF file
... The basal rate of H excretion was monitored for 5min, then 100|xM was added to measure the contribution of protons from plasma membrane ...
... The basal rate of H excretion was monitored for 5min, then 100|xM was added to measure the contribution of protons from plasma membrane ...
Principles of Virology
... • A susceptible cell has a functional receptor for a given virus - the cell may or may not be able to support viral replication • A resistant cell has no receptor -it may or may not be competent to support viral replication • A permissive cell has the capacity to replicate virus - it may or may not ...
... • A susceptible cell has a functional receptor for a given virus - the cell may or may not be able to support viral replication • A resistant cell has no receptor -it may or may not be competent to support viral replication • A permissive cell has the capacity to replicate virus - it may or may not ...
Growth-induced Microcracking and Repair Mechanisms of Fruit
... contamination and other external stressors [1-4]. Generally, this cuticular layer is made up of epicuticular wax (non-polar organic solvent-extractable wax) embedded within a matrix of solvent-insoluble cutin composed of covalently cross-linked hydroxy and hydroxyl-epoxy fatty acids, all produced by ...
... contamination and other external stressors [1-4]. Generally, this cuticular layer is made up of epicuticular wax (non-polar organic solvent-extractable wax) embedded within a matrix of solvent-insoluble cutin composed of covalently cross-linked hydroxy and hydroxyl-epoxy fatty acids, all produced by ...
Activation of Metallothionein Gene Expression
... cellular proliferation and apoptosis (6 –9), perhaps through an interaction of MT with nuclear factor-kB-DNA complexes (10). These properties of MTs reflect their potential importance for malignant progression; high expression of MTs correlates with poor prognosis and progressive disease in a number ...
... cellular proliferation and apoptosis (6 –9), perhaps through an interaction of MT with nuclear factor-kB-DNA complexes (10). These properties of MTs reflect their potential importance for malignant progression; high expression of MTs correlates with poor prognosis and progressive disease in a number ...
Modification of the signal sequence cleavage site of
... Fig. 1). Thus, a new SS cleavage site was created in the mutant protein three residues downstream of the natural cleavage site (deleted). Notably, the SignalP program also predicted a cleavage between residues RA and SA for the mutant protein. This alternative cleavage site respects the rule of spec ...
... Fig. 1). Thus, a new SS cleavage site was created in the mutant protein three residues downstream of the natural cleavage site (deleted). Notably, the SignalP program also predicted a cleavage between residues RA and SA for the mutant protein. This alternative cleavage site respects the rule of spec ...
Chapter 7 Structural Organisation in Animals Question
... 1. Define Tissue. Answer: It is the group of similar cells along with intercellular substances Performing specific functioin. 2. What is organ system? Answer: When two or more organs perform a common function by their physical and/or chemical interaction they together form organ system. 3. Which tis ...
... 1. Define Tissue. Answer: It is the group of similar cells along with intercellular substances Performing specific functioin. 2. What is organ system? Answer: When two or more organs perform a common function by their physical and/or chemical interaction they together form organ system. 3. Which tis ...
Every B3 Past Paper Question
... This method for cloning animals is not the only one. Dolly the sheep was produced using a different cloning method. Describe one way the cloning method used to produce Dolly the sheep is different from the cloning method described in the stages above. ...
... This method for cloning animals is not the only one. Dolly the sheep was produced using a different cloning method. Describe one way the cloning method used to produce Dolly the sheep is different from the cloning method described in the stages above. ...
Biology - PCMBToday
... outer seed coat is known as tegmen and inner seed coat is termed as testa. The surface of the seed possesses a fine pore at one end called hilum and there is also a scar called micropyle where funiculus or stalk of the seed is borne. Some seeds show place of origin of seed coats called raphe and par ...
... outer seed coat is known as tegmen and inner seed coat is termed as testa. The surface of the seed possesses a fine pore at one end called hilum and there is also a scar called micropyle where funiculus or stalk of the seed is borne. Some seeds show place of origin of seed coats called raphe and par ...
Year 10 Biology GCSE Objectives
... eukaryotic cells do. I can use orders of magnitude to correctly order objects according to size. ...
... eukaryotic cells do. I can use orders of magnitude to correctly order objects according to size. ...
A New Paradigm in Cellular Biology Gerson, Ling, Cope, Sodi
... this time among the establishment.” (18) The “sodium pump” was widely thought to be fueled by adenosine triphosphate (ATP) (the only possible source of energy for such a pump). In the early 50’s Ling inactivated all energy systems in the cells, including ATP, by poisoning them. In spite of the res ...
... this time among the establishment.” (18) The “sodium pump” was widely thought to be fueled by adenosine triphosphate (ATP) (the only possible source of energy for such a pump). In the early 50’s Ling inactivated all energy systems in the cells, including ATP, by poisoning them. In spite of the res ...
Expression Sequences and Distribution of Two Primary Cell
... of L-CAM gradually diminished in the nervous system ...
... of L-CAM gradually diminished in the nervous system ...
Non-specific chemical inhibition of the Fanconi anemia pathway
... cancer chemotherapy. However, the effectiveness of platinum compounds is often tempered by the acquisition of cellular drug resistance. Until now, no pharmacological approach has successfully overcome cisplatin resistance in cancer treatment. Since the Fanconi anemia (FA) pathway is a DNA damage res ...
... cancer chemotherapy. However, the effectiveness of platinum compounds is often tempered by the acquisition of cellular drug resistance. Until now, no pharmacological approach has successfully overcome cisplatin resistance in cancer treatment. Since the Fanconi anemia (FA) pathway is a DNA damage res ...
Fission Yeast Bub1 Is a Mitotic Centromere Protein Essential for the
... is the number of colonies with a red sector covering at least half of the colony divided by the total number of white colonies plus half-sectored colonies. ...
... is the number of colonies with a red sector covering at least half of the colony divided by the total number of white colonies plus half-sectored colonies. ...
BIM1 Encodes a Microtubule-binding Protein in Yeast.
... the strains listed in Table 4. Diploids were selected using complementing auxotrophic markers and then were sporulated and dissected. In most cases, the strains carried pRB326, containing the wild-type TUB1 gene; therefore, before analyzing the double-mutant phenotype, cells that had lost the plasmi ...
... the strains listed in Table 4. Diploids were selected using complementing auxotrophic markers and then were sporulated and dissected. In most cases, the strains carried pRB326, containing the wild-type TUB1 gene; therefore, before analyzing the double-mutant phenotype, cells that had lost the plasmi ...
9700/04 - StudyGuide.PK
... (a) Describe the importance of ATP in cells, giving two examples of processes in which it is used. ...
... (a) Describe the importance of ATP in cells, giving two examples of processes in which it is used. ...
Living together in biofilms: the microbial cell factory and its
... obtain industrial products are typically those of single species, which allows the controlled growth conditions needed to maximize the production of the desired compound [59–61]. In biofilms intended for industrial applications (biofilm reactors), microbial cells are fixed on different supports by a ...
... obtain industrial products are typically those of single species, which allows the controlled growth conditions needed to maximize the production of the desired compound [59–61]. In biofilms intended for industrial applications (biofilm reactors), microbial cells are fixed on different supports by a ...
J153
... MPL, GDL and the reactant channels [6]. Water is generated at the cathode catalyst layer and then transported through the GDL and MPL to the cathode reactant channels, from which it is removed from the cell. Water in the membrane is transported from the anode to the cathode side by electro-osmotic d ...
... MPL, GDL and the reactant channels [6]. Water is generated at the cathode catalyst layer and then transported through the GDL and MPL to the cathode reactant channels, from which it is removed from the cell. Water in the membrane is transported from the anode to the cathode side by electro-osmotic d ...
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... that flank the V, D and J gene segments, and join cleaved ends by non-‐homologous end joining (NHEJ). After rearrangement is complete, cells become pre-‐B cells, expressing the Igμ h ...
... that flank the V, D and J gene segments, and join cleaved ends by non-‐homologous end joining (NHEJ). After rearrangement is complete, cells become pre-‐B cells, expressing the Igμ h ...
silicone rubber in a parallel plate flow chamber in the
... summarized studies reporting increased as well as decreased growth rates of bacteria on surfaces as compared to planktonic bacteria, while increased adhesion of metabolically active cells was also found. The mechanisms of surface-associated changes in bacterial behaviour are probably related to nutr ...
... summarized studies reporting increased as well as decreased growth rates of bacteria on surfaces as compared to planktonic bacteria, while increased adhesion of metabolically active cells was also found. The mechanisms of surface-associated changes in bacterial behaviour are probably related to nutr ...
Myc increases self-renewal in neural progenitor cells through Miz-1
... Fig. 3. Myc overexpression does not block differentiation but delays the cell cycle exit of neural cells. (A) After 4 days in 2% FCS both Myc-overexpressing and control cells expressed the differentiation markers GFAP and TUJ-1 although the morphology of the Myc NPCs varies. (B) The expression of th ...
... Fig. 3. Myc overexpression does not block differentiation but delays the cell cycle exit of neural cells. (A) After 4 days in 2% FCS both Myc-overexpressing and control cells expressed the differentiation markers GFAP and TUJ-1 although the morphology of the Myc NPCs varies. (B) The expression of th ...
silicone rubber in a parallel plate flow chamber in the absence and
... summarized studies reporting increased as well as decreased growth rates of bacteria on surfaces as compared to planktonic bacteria, while increased adhesion of metabolically active cells was also found. The mechanisms of surface-associated changes in bacterial behaviour are probably related to nutr ...
... summarized studies reporting increased as well as decreased growth rates of bacteria on surfaces as compared to planktonic bacteria, while increased adhesion of metabolically active cells was also found. The mechanisms of surface-associated changes in bacterial behaviour are probably related to nutr ...
Suppression of inducible nitric oxide synthase expression in RAW
... Cell-free extracts as enzyme sources were prepared from the RAW 264.7 cells cultured for 16–18 h in the presence of test compounds and stimulators, lipopolysaccharide Ž1 mgrml. and interferon-g Ž10 Urml., using the method of Hayashi et al. Ž1997. with minor modifications. Specific activity of iNOS w ...
... Cell-free extracts as enzyme sources were prepared from the RAW 264.7 cells cultured for 16–18 h in the presence of test compounds and stimulators, lipopolysaccharide Ž1 mgrml. and interferon-g Ž10 Urml., using the method of Hayashi et al. Ž1997. with minor modifications. Specific activity of iNOS w ...
Effect of Gibberellic Acid and Actinomycin D on the Formation and
... does not grant permission for this article to be further copied/distributed or hosted elsewhere without the express permission from American Society of Plant Biologists. ...
... does not grant permission for this article to be further copied/distributed or hosted elsewhere without the express permission from American Society of Plant Biologists. ...
Responses Mediated Downregulation of B Cell
... were 80–85% CD19+ by cytofluorimetric analysis. After the isolation procedure was completed, cells were maintained in PBS for 3 h at 4˚C to minimize potential effects of anti-CD19 Abs on B cell activation. In a preliminary series of experiments, macrophages were removed by adherence, and B cells wer ...
... were 80–85% CD19+ by cytofluorimetric analysis. After the isolation procedure was completed, cells were maintained in PBS for 3 h at 4˚C to minimize potential effects of anti-CD19 Abs on B cell activation. In a preliminary series of experiments, macrophages were removed by adherence, and B cells wer ...
Cell culture
![](https://commons.wikimedia.org/wiki/Special:FilePath/Cell_Culture_in_a_tiny_Petri_dish.jpg?width=300)
Cell culture is the process by which cells are grown under controlled conditions, generally outside of their natural environment. In practice, the term ""cell culture"" now refers to the culturing of cells derived from multicellular eukaryotes, especially animal cells, in contrast with other types of culture that also grow cells, such as plant tissue culture, fungal culture, and microbiological culture (of microbes). The historical development and methods of cell culture are closely interrelated to those of tissue culture and organ culture. Viral culture is also related, with cells as hosts for the viruses. The laboratory technique of maintaining live cell lines (a population of cells descended from a single cell and containing the same genetic makeup) separated from their original tissue source became more robust in the middle 20th century.