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- RichardWheeler.net
... Three-dimensional electron microscopy tools have revolutionized our understanding of cell structure and molecular complexes in biology. Here, we describe methods for studying flagellar ultrastructure and biogenesis in two unicellular parasitesdTrypanosoma brucei and Leishmania mexicana. We describe ...
... Three-dimensional electron microscopy tools have revolutionized our understanding of cell structure and molecular complexes in biology. Here, we describe methods for studying flagellar ultrastructure and biogenesis in two unicellular parasitesdTrypanosoma brucei and Leishmania mexicana. We describe ...
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... eRNAi complements the global affects of pRNAi by facilitating gene knockdown in specific cells, in the context of other cells in the tissue, and at later developmental stages. Two different approaches have been developed to knock down gene expression using eRNAi in Parasteatoda. Pechmann and colleag ...
... eRNAi complements the global affects of pRNAi by facilitating gene knockdown in specific cells, in the context of other cells in the tissue, and at later developmental stages. Two different approaches have been developed to knock down gene expression using eRNAi in Parasteatoda. Pechmann and colleag ...
Diffusion of Green Fluorescent Protein in Three Cell
... rates at which proteins and other cell components can diffuse is crucial for the understanding of a cell as a physical system. There have been numerous measurements of diffusion coefficients in eukaryotic cells by using fluorescence recovery after photobleaching (FRAP) and related techniques. Much l ...
... rates at which proteins and other cell components can diffuse is crucial for the understanding of a cell as a physical system. There have been numerous measurements of diffusion coefficients in eukaryotic cells by using fluorescence recovery after photobleaching (FRAP) and related techniques. Much l ...
the spider Parasteatoda tepidariorum
... eRNAi complements the global affects of pRNAi by facilitating gene knockdown in specific cells, in the context of other cells in the tissue, and at later developmental stages. Two different approaches have been developed to knock down gene expression using eRNAi in Parasteatoda. Pechmann and colleag ...
... eRNAi complements the global affects of pRNAi by facilitating gene knockdown in specific cells, in the context of other cells in the tissue, and at later developmental stages. Two different approaches have been developed to knock down gene expression using eRNAi in Parasteatoda. Pechmann and colleag ...
Redox Homeostasis and Antioxidant Signaling: A
... and photochemistry. Numerous enzyme systems produce superoxide or H2O2. ...
... and photochemistry. Numerous enzyme systems produce superoxide or H2O2. ...
Hybridoma and Hybridomics
... Antibodies are an excellent tool for the detection of the expressed enzyme in situ and for the separation of the relevant organism for further phylogenetical investigations. Thus, they should be helpful in determining the conditions that control the release of N2O, for example, in agricultural soils ...
... Antibodies are an excellent tool for the detection of the expressed enzyme in situ and for the separation of the relevant organism for further phylogenetical investigations. Thus, they should be helpful in determining the conditions that control the release of N2O, for example, in agricultural soils ...
Protist predation can favour cooperation within bacterial species
... QS determines that they are released at high cell densities when they will be most beneficial [5]. Production of QS-dependent public goods can be exploited by cheats which do not produce the costly public goods because they do not respond to QS signal molecules [6]. Recent studies have reported a be ...
... QS determines that they are released at high cell densities when they will be most beneficial [5]. Production of QS-dependent public goods can be exploited by cheats which do not produce the costly public goods because they do not respond to QS signal molecules [6]. Recent studies have reported a be ...
Dynamics of PhiX174 protein E-mediated lysis of
... to penetrate the outer membrane and equipoise the periplasmic space. As sucrose cannot pass the inner membrane of E. coli, sucrose is often used to compensate the cytoplasmic tonicity (Osborn and Munson 1974). However, the concentrations of sucrose used for the determinations of the sucrose space we ...
... to penetrate the outer membrane and equipoise the periplasmic space. As sucrose cannot pass the inner membrane of E. coli, sucrose is often used to compensate the cytoplasmic tonicity (Osborn and Munson 1974). However, the concentrations of sucrose used for the determinations of the sucrose space we ...
39 | the respiratory system
... Partial pressure is a measure of the concentration of the individual components in a mixture of gases. The total pressure exerted by the mixture is the sum of the partial pressures of the components in the mixture. The rate of diffusion of a gas is proportional to its partial pressure within the tot ...
... Partial pressure is a measure of the concentration of the individual components in a mixture of gases. The total pressure exerted by the mixture is the sum of the partial pressures of the components in the mixture. The rate of diffusion of a gas is proportional to its partial pressure within the tot ...
PPT - Altogen Biosystems
... Altogen Biosystems offers the A431 Transfection Reagent among a host of 100+ cell line specific In Vitro Transfection Kits. A431 Transfection Reagent is an advanced formulation of a cationic lipid-based reagent, and it is developed to provide high transfection efficiency with A431 and other skin can ...
... Altogen Biosystems offers the A431 Transfection Reagent among a host of 100+ cell line specific In Vitro Transfection Kits. A431 Transfection Reagent is an advanced formulation of a cationic lipid-based reagent, and it is developed to provide high transfection efficiency with A431 and other skin can ...
Effects of Lignification, Cellulose Crystallinity and Enzyme
... cellulose pol ymers of the microfibril, which in turn prevents cellul ose hydrolys is by cellulol ytic enzymes or microorganisms . As a result, microbes are limited to a tt ac king cellulose polymers on the outer surface of the unhydr a tcd microfibril uni t. Haworth e t al. (1969) noted that 44 % o ...
... cellulose pol ymers of the microfibril, which in turn prevents cellul ose hydrolys is by cellulol ytic enzymes or microorganisms . As a result, microbes are limited to a tt ac king cellulose polymers on the outer surface of the unhydr a tcd microfibril uni t. Haworth e t al. (1969) noted that 44 % o ...
Some Characteristics of an Early Protein (ICP 22) Synthesized in
... unabated during this period. Other polypeptides not discussed here are identified in Fig. 5 in order to allow comparison with earlier work using the same electrophoresis system. Modification of lCP 22 The autoradiograms in Fig. 3 and 4 suggested that the post-translational modification of ICP 22 to ...
... unabated during this period. Other polypeptides not discussed here are identified in Fig. 5 in order to allow comparison with earlier work using the same electrophoresis system. Modification of lCP 22 The autoradiograms in Fig. 3 and 4 suggested that the post-translational modification of ICP 22 to ...
Brief rapid pacing depresses contractile function via Ca
... Received 23 May 2000; accepted in final form 15 August 2000 ...
... Received 23 May 2000; accepted in final form 15 August 2000 ...
Restricted, canonical, stereotyped and convergent immunoglobulin
... that is, responses that induce essentially identical immunoglobulins from different mice, despite the massive potential diversity of VDJ rearrangement. For example, B-cell responses of C57BL/6 mice to NP select for canonical antibodies encoded by V186.2 recombined to DFI 16.1 D segment and the JH1 J ...
... that is, responses that induce essentially identical immunoglobulins from different mice, despite the massive potential diversity of VDJ rearrangement. For example, B-cell responses of C57BL/6 mice to NP select for canonical antibodies encoded by V186.2 recombined to DFI 16.1 D segment and the JH1 J ...
emboj7601781-sup
... XRab40 cDNA fragments were amplified by PCR using the XL044o08 clone in the NIBB Xenopus database (http://xenopus.nibb.ac.jp/) as a template. To express XRab40 in Xenopus embryos and tissue culture cells, the fragments were cloned into pCS2+ or pCS2+-based epitope tagging vectors. XRab40ΔSOCS lacks ...
... XRab40 cDNA fragments were amplified by PCR using the XL044o08 clone in the NIBB Xenopus database (http://xenopus.nibb.ac.jp/) as a template. To express XRab40 in Xenopus embryos and tissue culture cells, the fragments were cloned into pCS2+ or pCS2+-based epitope tagging vectors. XRab40ΔSOCS lacks ...
Brief rapid pacing depresses contractile function via Ca - AJP
... Received 23 May 2000; accepted in final form 15 August 2000 ...
... Received 23 May 2000; accepted in final form 15 August 2000 ...
β Glucocorticoids on IL-1 Counteract the Inhibitory Effect of Migration
... Human PBMC were cultured in RPMI medium with inclusion or exclusion of 10 nM dexamethasone in the absence of LPS (Fig. 2), or in the presence of LPS (Fig. 4). Four hours before staining with cellular surface marker, monensin (GolgiStop; BD PharMingen) that enhanced intracellular cytokine accumulatio ...
... Human PBMC were cultured in RPMI medium with inclusion or exclusion of 10 nM dexamethasone in the absence of LPS (Fig. 2), or in the presence of LPS (Fig. 4). Four hours before staining with cellular surface marker, monensin (GolgiStop; BD PharMingen) that enhanced intracellular cytokine accumulatio ...
Sos recruitment system for the analysis of the
... sterile wire loop and streaking the splinters onto a YPAD (yeastextract,peptone,adenine,an d dextrose) agar plate. At the same time, each of 4 “dropout” plates was stocked with agar yeast streaked from -80°C glycerol. The dropout plates were made using appropriate 10X dropout solutions and agar plat ...
... sterile wire loop and streaking the splinters onto a YPAD (yeastextract,peptone,adenine,an d dextrose) agar plate. At the same time, each of 4 “dropout” plates was stocked with agar yeast streaked from -80°C glycerol. The dropout plates were made using appropriate 10X dropout solutions and agar plat ...
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... smooth muscle; these invading mesenchyme cells did not, however, form smooth muscle. In all cultured fragments the epithelium overgrew the ends of the tube and covered the surface of the whole fragment, but this overgrowth appeared to have no effect on folding of the epithelium. The conclusion that ...
... smooth muscle; these invading mesenchyme cells did not, however, form smooth muscle. In all cultured fragments the epithelium overgrew the ends of the tube and covered the surface of the whole fragment, but this overgrowth appeared to have no effect on folding of the epithelium. The conclusion that ...
Effect of topical rebamipide on goblet cells in the lid wiper of human
... surface conditions such as lid wiper epitheliopathy. The aim of the present study was to analyze the changes in goblet cell number, cell proliferation, and epidermal growth factor receptor (EGFR) induced by topical rebamipide addition to the lid wiper of humans. A total of 30 eyelid tissue samples w ...
... surface conditions such as lid wiper epitheliopathy. The aim of the present study was to analyze the changes in goblet cell number, cell proliferation, and epidermal growth factor receptor (EGFR) induced by topical rebamipide addition to the lid wiper of humans. A total of 30 eyelid tissue samples w ...
Morphogenesis of intestinal villi
... smooth muscle; these invading mesenchyme cells did not, however, form smooth muscle. In all cultured fragments the epithelium overgrew the ends of the tube and covered the surface of the whole fragment, but this overgrowth appeared to have no effect on folding of the epithelium. The conclusion that ...
... smooth muscle; these invading mesenchyme cells did not, however, form smooth muscle. In all cultured fragments the epithelium overgrew the ends of the tube and covered the surface of the whole fragment, but this overgrowth appeared to have no effect on folding of the epithelium. The conclusion that ...
Cell cycle exit during terminal erythroid differentiation is associated
... From www.bloodjournal.org by guest on June 11, 2017. For personal use only. BLOOD, 15 OCTOBER 2000 䡠 VOLUME 96, NUMBER 8 ...
... From www.bloodjournal.org by guest on June 11, 2017. For personal use only. BLOOD, 15 OCTOBER 2000 䡠 VOLUME 96, NUMBER 8 ...
THE IMMUNE SYSTEM
... n some cases, like in the case of Type 1 Diabetes (Juvenile Diabetes), the body does not have enough insulin, so the cells can’t absorb sugars as well and the sugar level in the blood starts to rise. The body becomes tired, weak, dehydrated and blood pressure lowers, causing a coma if not treated qu ...
... n some cases, like in the case of Type 1 Diabetes (Juvenile Diabetes), the body does not have enough insulin, so the cells can’t absorb sugars as well and the sugar level in the blood starts to rise. The body becomes tired, weak, dehydrated and blood pressure lowers, causing a coma if not treated qu ...
Cell culture
![](https://commons.wikimedia.org/wiki/Special:FilePath/Cell_Culture_in_a_tiny_Petri_dish.jpg?width=300)
Cell culture is the process by which cells are grown under controlled conditions, generally outside of their natural environment. In practice, the term ""cell culture"" now refers to the culturing of cells derived from multicellular eukaryotes, especially animal cells, in contrast with other types of culture that also grow cells, such as plant tissue culture, fungal culture, and microbiological culture (of microbes). The historical development and methods of cell culture are closely interrelated to those of tissue culture and organ culture. Viral culture is also related, with cells as hosts for the viruses. The laboratory technique of maintaining live cell lines (a population of cells descended from a single cell and containing the same genetic makeup) separated from their original tissue source became more robust in the middle 20th century.