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PPT - Altogen Biosystems
... NIH3T3 Transfection Reagent (Swiss Mouse Fibroblasts) Altogen Biosystems offers the NIH3T3 Transfection Reagent among a host of 100+ cell line specific In Vitro Transfection Kits. NIH3T3 Transfection Reagent is an Proprietary cationic lipids formulation, and it is developed to provide high transfect ...
... NIH3T3 Transfection Reagent (Swiss Mouse Fibroblasts) Altogen Biosystems offers the NIH3T3 Transfection Reagent among a host of 100+ cell line specific In Vitro Transfection Kits. NIH3T3 Transfection Reagent is an Proprietary cationic lipids formulation, and it is developed to provide high transfect ...
Cytotoxicity and inhibition of platelet aggregation
... individual LAAOs differ in their structure, substrate specificity as well as in their biological properties. Thus, ophidian LAAO has become an attractive target for research in molecular biochemistry, physiology and medicine due to its multiple effects on several cells. The pit viper Bothrops leucuru ...
... individual LAAOs differ in their structure, substrate specificity as well as in their biological properties. Thus, ophidian LAAO has become an attractive target for research in molecular biochemistry, physiology and medicine due to its multiple effects on several cells. The pit viper Bothrops leucuru ...
Oocyte Development in Hydra Involves Selection from Competent
... differentiate into oocytes. The oocyte is singled out from this subset and the competence of the remaining cells to become oocytes dramatically decreases as they adopt the alternative nurse cell fate. Progression through the nurse cell differentiation program requires the presence of the oocyte. Whe ...
... differentiate into oocytes. The oocyte is singled out from this subset and the competence of the remaining cells to become oocytes dramatically decreases as they adopt the alternative nurse cell fate. Progression through the nurse cell differentiation program requires the presence of the oocyte. Whe ...
The Cellular Basis of Gastrulation in Xenopus laevis: Active
... (Nakatsuji, 1975, 1976; Keller and Schoenwolf, 1977) and carry the archenteron roof with them (Keller, 1981). More accurately, the involuted mesoderm pulls the preinvolution material vegetally, over itself. In urodeles, bottle cells were thought to migrate into the interior of the gastrula and pull ...
... (Nakatsuji, 1975, 1976; Keller and Schoenwolf, 1977) and carry the archenteron roof with them (Keller, 1981). More accurately, the involuted mesoderm pulls the preinvolution material vegetally, over itself. In urodeles, bottle cells were thought to migrate into the interior of the gastrula and pull ...
Figures and figure supplements
... Figure 5. Exogenous tryptophan promotes bacterial IAA biosynthesis. (a) Addition of 0.1 mM tryptophan to P. inhibens cultures results in approximately 25-fold increase in produced IAA. Shown is an LC-MS extracted ion chromatogram (EIC, m/z 176.0706 ± 10 ppm) of an IAA standard of 10 mM (black), IAA ...
... Figure 5. Exogenous tryptophan promotes bacterial IAA biosynthesis. (a) Addition of 0.1 mM tryptophan to P. inhibens cultures results in approximately 25-fold increase in produced IAA. Shown is an LC-MS extracted ion chromatogram (EIC, m/z 176.0706 ± 10 ppm) of an IAA standard of 10 mM (black), IAA ...
Medullary thymic epithelial cell depletion leads to autoimmune
... of plasma cells and anti-nuclear autoantibodies (ANAs). In addition to the histological manifestations discussed above, development of AIH in humans correlates with increased presence of plasma cells in intrahepatic inflammatory lesions as well as elevated total serum Ig levels and organ-specific an ...
... of plasma cells and anti-nuclear autoantibodies (ANAs). In addition to the histological manifestations discussed above, development of AIH in humans correlates with increased presence of plasma cells in intrahepatic inflammatory lesions as well as elevated total serum Ig levels and organ-specific an ...
PDF
... The environment is also a strong determinant on phytoplankton host-virus interactions (Mojica and Brussaard, 2014). Regarding the important role of marine viruses as drivers of biogeochemical cycling (Suttle, 2007; Brussaard et al., 2008), the indirect effects (i.e., via the host) of the environment ...
... The environment is also a strong determinant on phytoplankton host-virus interactions (Mojica and Brussaard, 2014). Regarding the important role of marine viruses as drivers of biogeochemical cycling (Suttle, 2007; Brussaard et al., 2008), the indirect effects (i.e., via the host) of the environment ...
Compounds from Wild Mushrooms with Antitumor
... the last two decades and scientific explanation to show how compounds derived from mushrooms function in humans are increasingly being established [12]. Fungal fruiting bodies, fungal mycelium or the culture fluid in which the mycelium has been cultivated may all be explored for biological activity. ...
... the last two decades and scientific explanation to show how compounds derived from mushrooms function in humans are increasingly being established [12]. Fungal fruiting bodies, fungal mycelium or the culture fluid in which the mycelium has been cultivated may all be explored for biological activity. ...
Polarity and endocytosis: reciprocal regulation
... Drosophila, and also complicated by its additional role in polarized biosynthetic delivery pathways [28,29]. The polarity phenotypes associated with loss of ESCRT proteins, which would be expected to have their major impact on sorting of cargo destined for degradation, lend some weight to the requir ...
... Drosophila, and also complicated by its additional role in polarized biosynthetic delivery pathways [28,29]. The polarity phenotypes associated with loss of ESCRT proteins, which would be expected to have their major impact on sorting of cargo destined for degradation, lend some weight to the requir ...
Influence of salinity on the localization of Na+/K+
... indicated that these were mitochondrion-rich chloride cells. No Chloride cells on the secondary lamellae were more flattened other cell types in the gill were stained above background (less round with a lower shape factor; Table·1) than those on the primary filament. Shape of chloride cells on the p ...
... indicated that these were mitochondrion-rich chloride cells. No Chloride cells on the secondary lamellae were more flattened other cell types in the gill were stained above background (less round with a lower shape factor; Table·1) than those on the primary filament. Shape of chloride cells on the p ...
Malaysian Guidelines for Stem Cell Research and Therapy
... The 2nd edition of the National Guidelines for Stem Cell Research and Therapy, prepared by the Medical Development Division of the Ministry of Health, has comprehensively revised the first edition of the same book to facilitate researchers and clinicians involved in stem cell research and therapy. S ...
... The 2nd edition of the National Guidelines for Stem Cell Research and Therapy, prepared by the Medical Development Division of the Ministry of Health, has comprehensively revised the first edition of the same book to facilitate researchers and clinicians involved in stem cell research and therapy. S ...
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... 1989), exploited the fact that cell division cycle mutants tended to die in the late larval or early pupal stages. This is because the maternal contribution of cell cycle proteins supports the rapid syncytial nuclear division cycles and the subsequent embryonic cell cycles. Development through the l ...
... 1989), exploited the fact that cell division cycle mutants tended to die in the late larval or early pupal stages. This is because the maternal contribution of cell cycle proteins supports the rapid syncytial nuclear division cycles and the subsequent embryonic cell cycles. Development through the l ...
The hypersensitive response is associated with host and nonhost
... is prevented from further growth, resulting in resistance. This accelerated localized plant cell death response is de®ned as the HR. In compatible interactions, the HR is not induced or is induced to a lesser extent and a biotrophic relation can be established, resulting in plant disease. Race-nonsp ...
... is prevented from further growth, resulting in resistance. This accelerated localized plant cell death response is de®ned as the HR. In compatible interactions, the HR is not induced or is induced to a lesser extent and a biotrophic relation can be established, resulting in plant disease. Race-nonsp ...
chapt01_lecture
... units made of two or more primary tissues Systems are groups of organs working together to maintain homeostasis ...
... units made of two or more primary tissues Systems are groups of organs working together to maintain homeostasis ...
Merotelic kinetochore orientation occurs frequently during early
... We next tested for the incidence of merotelic orientation in prometaphase of cells recovering from a nocodazoleinduced mitotic block because of the higher incidence of anaphase lagging chromosomes compared to controls (Cimini et al., 2001; Cimini et al., 1999). Cells were released from the mitotic b ...
... We next tested for the incidence of merotelic orientation in prometaphase of cells recovering from a nocodazoleinduced mitotic block because of the higher incidence of anaphase lagging chromosomes compared to controls (Cimini et al., 2001; Cimini et al., 1999). Cells were released from the mitotic b ...
TR3 is preferentially expressed by bulge epithelial stem
... Figure 1 TR3 expression in human hair follicles. (a) qRT-PCR analysis of TR3 expression in anagen bulb matrix and telogen bulge. *Po0.05. Experiments were performed in triplicate at least three times. (b, left) Expression of TR3 protein in human hair follicle from scalp tissue by immunohistochemistr ...
... Figure 1 TR3 expression in human hair follicles. (a) qRT-PCR analysis of TR3 expression in anagen bulb matrix and telogen bulge. *Po0.05. Experiments were performed in triplicate at least three times. (b, left) Expression of TR3 protein in human hair follicle from scalp tissue by immunohistochemistr ...
Study of adhesive and invasion capacity of some opportunistic
... determine in certain conditions the occurrence of infectious diseases. A great number of E. coli strains could induce a great variety of infectious diseases in adult and infant population as: intestinal, urinary tract infections or septicemia. Opportunistic and pathogenic bacteria exhibit specific a ...
... determine in certain conditions the occurrence of infectious diseases. A great number of E. coli strains could induce a great variety of infectious diseases in adult and infant population as: intestinal, urinary tract infections or septicemia. Opportunistic and pathogenic bacteria exhibit specific a ...
Induction of Kranz Anatomy and C4-like
... Both the terrestrial and submerged forms accumulated the large subunit of Rubisco in the chloroplasts in all three types of photosynthetic cells (Table 1). Thus, the terrestrial form differed from typical C4 plants in the cellular distribution of Rubisco (Ueno, 1996b). This cellular distribution of ...
... Both the terrestrial and submerged forms accumulated the large subunit of Rubisco in the chloroplasts in all three types of photosynthetic cells (Table 1). Thus, the terrestrial form differed from typical C4 plants in the cellular distribution of Rubisco (Ueno, 1996b). This cellular distribution of ...
The Cortical Microtubule Array: From Dynamics to
... regulated changes of their assembly dynamics. The direct regulation of microtubule assembly dynamics typically is achieved through the activity of MAPs; therefore, a key objective is to understand the mechanisms that regulate MAP activity and how these mechanisms are triggered in response to develop ...
... regulated changes of their assembly dynamics. The direct regulation of microtubule assembly dynamics typically is achieved through the activity of MAPs; therefore, a key objective is to understand the mechanisms that regulate MAP activity and how these mechanisms are triggered in response to develop ...
One-eyed pinhead regulates cell motility independent of Squint
... Morphologically abnormal cells in the hypoblast of oep mutants To elucidate the role of oep in morphogenesis, we labeled individual precursor cells of the mesendoderm with lineage tracer in wild-type and oep mutant blastulae and examined the morphology of the clonal progeny in the late gastrulae. In ...
... Morphologically abnormal cells in the hypoblast of oep mutants To elucidate the role of oep in morphogenesis, we labeled individual precursor cells of the mesendoderm with lineage tracer in wild-type and oep mutant blastulae and examined the morphology of the clonal progeny in the late gastrulae. In ...
Chapter 5
... Defined (synthetic): Know all components and their concentrations Complex: contain some ingredients of unknown composition and/or concentration (supplies amino acids, vitamins, growth factors…..) Function ...
... Defined (synthetic): Know all components and their concentrations Complex: contain some ingredients of unknown composition and/or concentration (supplies amino acids, vitamins, growth factors…..) Function ...
- Wiley Online Library
... could be used to overcome these detrimental changes. STEM CELLS 2012;30:1182–1195 ...
... could be used to overcome these detrimental changes. STEM CELLS 2012;30:1182–1195 ...
Recruitment of lymphocytes to the human liver
... fenestrated endothelium that lines the sinusoids. However, there is also evidence for differences between portal and hepatic venous endothelium and differences within the sinusoids.5–9 The sinusoidal endothelial cells (SEC) are morphologically unique and are characterized by an absence of tight junc ...
... fenestrated endothelium that lines the sinusoids. However, there is also evidence for differences between portal and hepatic venous endothelium and differences within the sinusoids.5–9 The sinusoidal endothelial cells (SEC) are morphologically unique and are characterized by an absence of tight junc ...
Goblet Cells Contribute to Ocular Surface Immune Tolerance
... conjunctiva. (Figure 1A). Antigens, including 2.3 kDa OVA peptide, and 10 and 70 kDa dextrans, passed into the stroma through goblet cell associated passages (GAPs) in WT (Figure 1A). The least epithelial retention was noted for OVA peptide, and the greatest stromal localization was noted for the tw ...
... conjunctiva. (Figure 1A). Antigens, including 2.3 kDa OVA peptide, and 10 and 70 kDa dextrans, passed into the stroma through goblet cell associated passages (GAPs) in WT (Figure 1A). The least epithelial retention was noted for OVA peptide, and the greatest stromal localization was noted for the tw ...
UVB induced cell cycle checkpoints in an early stage
... S phase fell from 33 to 5% at 12 h post-UVB, with a nadir of 2 ± 3% S phase cells by 18 ± 24 h. Recovery of G1-to-S phase progression was seen between 24 and 36 h. Cells that had a DNA content between 2N and 4N before irradiation failed to progress into G2, indicating inhibition of replicative DNA s ...
... S phase fell from 33 to 5% at 12 h post-UVB, with a nadir of 2 ± 3% S phase cells by 18 ± 24 h. Recovery of G1-to-S phase progression was seen between 24 and 36 h. Cells that had a DNA content between 2N and 4N before irradiation failed to progress into G2, indicating inhibition of replicative DNA s ...
Cell culture
![](https://commons.wikimedia.org/wiki/Special:FilePath/Cell_Culture_in_a_tiny_Petri_dish.jpg?width=300)
Cell culture is the process by which cells are grown under controlled conditions, generally outside of their natural environment. In practice, the term ""cell culture"" now refers to the culturing of cells derived from multicellular eukaryotes, especially animal cells, in contrast with other types of culture that also grow cells, such as plant tissue culture, fungal culture, and microbiological culture (of microbes). The historical development and methods of cell culture are closely interrelated to those of tissue culture and organ culture. Viral culture is also related, with cells as hosts for the viruses. The laboratory technique of maintaining live cell lines (a population of cells descended from a single cell and containing the same genetic makeup) separated from their original tissue source became more robust in the middle 20th century.