Imaging the fate of histone Cse4 reveals de novo replacement in S
... of centromere–kinetochore interactions. Despite this simplicity, the architecture of Cse4 nucleosomes has become the subject of much debate. Cse4 nucleosomes have been reported to differ from the canonical nucleosome not only by the replacement of both molecules of histone H3 by the Cse4 variant, bu ...
... of centromere–kinetochore interactions. Despite this simplicity, the architecture of Cse4 nucleosomes has become the subject of much debate. Cse4 nucleosomes have been reported to differ from the canonical nucleosome not only by the replacement of both molecules of histone H3 by the Cse4 variant, bu ...
cilia regeneration in tetrahymena and its inhibition
... The cilia of Tetrahymena were amputated by the use of a procedure in which the cells remained viable and regenerated cilia. Deciliated cells were nonmotile, and cilia regeneration was assessed by scoring the percentage of motile cells at intervals following deciliation. After a 30-min lag, the decil ...
... The cilia of Tetrahymena were amputated by the use of a procedure in which the cells remained viable and regenerated cilia. Deciliated cells were nonmotile, and cilia regeneration was assessed by scoring the percentage of motile cells at intervals following deciliation. After a 30-min lag, the decil ...
Rab-A2 and Rab-A3 GTPases Define a trans
... Arabidopsis Rab-A2 and Rab-A3 Proteins For localization studies, we constructed YFP fusions with genomic DNA fragments from all four members of the Rab-A2 subclass (RAB-A2a, -A2b, -A2c, and -A2d) and with the single Rab-A3 protein (RAB-A3). These DNA fragments included the entire intergenic region w ...
... Arabidopsis Rab-A2 and Rab-A3 Proteins For localization studies, we constructed YFP fusions with genomic DNA fragments from all four members of the Rab-A2 subclass (RAB-A2a, -A2b, -A2c, and -A2d) and with the single Rab-A3 protein (RAB-A3). These DNA fragments included the entire intergenic region w ...
Progressive divergence of definitive haematopoietic stem cells from
... expressed KIT. However, in contrast to both the endothelial and haematopoietic lineages, this fraction was greatly enriched for KIThigh cells. Unlike the haematopoietic population, all endothelial and DP cells expressed low levels of SCA1. AA4.1 and CD34, also associated with the embryonic HSCs phen ...
... expressed KIT. However, in contrast to both the endothelial and haematopoietic lineages, this fraction was greatly enriched for KIThigh cells. Unlike the haematopoietic population, all endothelial and DP cells expressed low levels of SCA1. AA4.1 and CD34, also associated with the embryonic HSCs phen ...
Electrocardioqraphic criteria for predicting the site of coronary artery
... The shutting off ; after the plasmid has established itself in the recipient cell results in shutting off the activity of the pheromone by two functions encoded on the plasmid; one involves a reduction of the pheromone production, and other by production of specific inhibitor peptide which encoded b ...
... The shutting off ; after the plasmid has established itself in the recipient cell results in shutting off the activity of the pheromone by two functions encoded on the plasmid; one involves a reduction of the pheromone production, and other by production of specific inhibitor peptide which encoded b ...
Pausing of Golgi Bodies on Microtubules Regulates
... images (Figures 1P and 1Q). In addition to Golgi bodies, we identified a second, relatively rare population (<1 observed per cell per 10 min imaging period) of smaller, homogeneously labeled particles with the same rapid, nonlinear movement as Golgi bodies (Figures 1J and 1K). These particles were i ...
... images (Figures 1P and 1Q). In addition to Golgi bodies, we identified a second, relatively rare population (<1 observed per cell per 10 min imaging period) of smaller, homogeneously labeled particles with the same rapid, nonlinear movement as Golgi bodies (Figures 1J and 1K). These particles were i ...
Physiological and Morphological Characteristics of
... stationary phase cultures (Thomson et al., 1972; Lecroisey et al., 1975). Since enzymes are available in shaken cultures for the degradation and recycling of amino acids from extracellular proteins, the effect of aeration on an amino acid transport system was investigated. Furthermore, the possibili ...
... stationary phase cultures (Thomson et al., 1972; Lecroisey et al., 1975). Since enzymes are available in shaken cultures for the degradation and recycling of amino acids from extracellular proteins, the effect of aeration on an amino acid transport system was investigated. Furthermore, the possibili ...
Visualization of the moonlighting protein CD26DPPIV - UvA-DARE
... produce maximal amounts of colored or fluorescent final reaction product. These concentrations are usually high so that the maximal velocity of the enzyme (Vmax) is obtained. However, these high substrate concentrations are seldom present in vivo. Moreover, the affinity of an enzyme for its substrat ...
... produce maximal amounts of colored or fluorescent final reaction product. These concentrations are usually high so that the maximal velocity of the enzyme (Vmax) is obtained. However, these high substrate concentrations are seldom present in vivo. Moreover, the affinity of an enzyme for its substrat ...
Catalases and PhoB/PhoR system independently contribute to
... cells electrophoretic profile- Fig. 1(c)) were excised, treated with trypsin and the resulting peptides ...
... cells electrophoretic profile- Fig. 1(c)) were excised, treated with trypsin and the resulting peptides ...
Melanotransferrin stimulates t-PA
... PA in degradation of the ECM during angiogenesis [23]. Plasmin is involved in the control of cell proliferation [24], apoptosis [21,25] and migration [24] during the remodeling of vessel walls. In addition, plasmin can affect the ECM organization through the proteolysis of some components such as fi ...
... PA in degradation of the ECM during angiogenesis [23]. Plasmin is involved in the control of cell proliferation [24], apoptosis [21,25] and migration [24] during the remodeling of vessel walls. In addition, plasmin can affect the ECM organization through the proteolysis of some components such as fi ...
The Balance between Cell Division and Endoreplication Depends
... scaffold (CUL1, ASK, and RBX) (del Pozo and Estelle, 2000) and the F-box subunit SKP2A that specifically interacts with the targets. Furthermore, E2FC degradation was triggered during the transition from dark to light growth conditions, suggesting that E2FC plays an important role in this transition ...
... scaffold (CUL1, ASK, and RBX) (del Pozo and Estelle, 2000) and the F-box subunit SKP2A that specifically interacts with the targets. Furthermore, E2FC degradation was triggered during the transition from dark to light growth conditions, suggesting that E2FC plays an important role in this transition ...
pdf: Baskin 2013
... some plant cell biologists have used apical for cell polarity with reference to the shoot apex only, so that under their terminology apical points away from the root’s apex; however, an alternative terminology for cell polarity has been recently proposed.4 In any case, here, anatomy rather than cell ...
... some plant cell biologists have used apical for cell polarity with reference to the shoot apex only, so that under their terminology apical points away from the root’s apex; however, an alternative terminology for cell polarity has been recently proposed.4 In any case, here, anatomy rather than cell ...
Studies on polar cell wall growth and antibiotic susceptibility of
... Understanding cellular processes in bacteria is the fundamental prerequisite for bacterial applications in biotechnology or medicine. The engineering of high efficient production strains as well as the treatment of bacterial pathogens have evolved to extremely important tasks in our society. Being f ...
... Understanding cellular processes in bacteria is the fundamental prerequisite for bacterial applications in biotechnology or medicine. The engineering of high efficient production strains as well as the treatment of bacterial pathogens have evolved to extremely important tasks in our society. Being f ...
The Bordetella pertussis adenylate cyclase toxin binds to T cells via
... The interaction of CyaA with T cells is mediated by LFA-1 To further characterize the interaction of CyaA with its putative receptor on T cells, we compared CyaA binding to quiescent and activated T cells. Peripheral T cells were stimulated at different times with an agonistic anti-CD3 mAb, and CyaA ...
... The interaction of CyaA with T cells is mediated by LFA-1 To further characterize the interaction of CyaA with its putative receptor on T cells, we compared CyaA binding to quiescent and activated T cells. Peripheral T cells were stimulated at different times with an agonistic anti-CD3 mAb, and CyaA ...
Effects of Glucocorticoid on Microglia Cell Functions
... MRs that were 80% occupied while G R was only 10% occupied. G R binding increased only when a higher level of corticosterone was introduced as is the case during stress (Reul and De Kloet, 1985; Reul et al., 1987). Differential binding by the two receptors in brain cells may play a role in the varie ...
... MRs that were 80% occupied while G R was only 10% occupied. G R binding increased only when a higher level of corticosterone was introduced as is the case during stress (Reul and De Kloet, 1985; Reul et al., 1987). Differential binding by the two receptors in brain cells may play a role in the varie ...
Endocytosis, Actin Cytoskeleton, and Signaling
... membrane proteins and lipids, extracellular molecules, fluids, particles, exosomes, viruses, and bacteria. Endocytic internalization is a conserved process for all eukaryotic cells that is required for diverse cellular functions. These include turnover and degradation of plasma membrane proteins and ...
... membrane proteins and lipids, extracellular molecules, fluids, particles, exosomes, viruses, and bacteria. Endocytic internalization is a conserved process for all eukaryotic cells that is required for diverse cellular functions. These include turnover and degradation of plasma membrane proteins and ...
Targeting Robo4-dependent slit signaling to survive the cytokine storm in sepsis and influenza. Science Trans Med 2, 1-9.
... immune responses. The marked and abrupt release of multiple cytokines by the immune system, often referred to as hypercytokinemia or cytokine storm, itself has disruptive effects on the host’s physiology. In many infections, components of the resulting cytokine-induced secondary inflammatory injury ...
... immune responses. The marked and abrupt release of multiple cytokines by the immune system, often referred to as hypercytokinemia or cytokine storm, itself has disruptive effects on the host’s physiology. In many infections, components of the resulting cytokine-induced secondary inflammatory injury ...
Phospholipase D promotes Arcanobacterium haemolyticum
... punctate-staining cells compared to untreated HeLa cells (9.9%; negative control; Figure 2D). In the presence of PLD, 26.0% of HeLa cells displayed punctate staining (positive control; p < 0.05 compared to the negative control; Figure 2D). In the presence of PLD, addition of pre-immune serum did not ...
... punctate-staining cells compared to untreated HeLa cells (9.9%; negative control; Figure 2D). In the presence of PLD, 26.0% of HeLa cells displayed punctate staining (positive control; p < 0.05 compared to the negative control; Figure 2D). In the presence of PLD, addition of pre-immune serum did not ...
Experimental taphonomy of giant sulphur bacteria: implications for
... compare them directly with living organisms [18]. Although patterns of decay in animal embryos are now being studied [17,19–22], nothing is known about decay processes or preservation potential in giant sulphur-oxidizing bacteria. Previous studies on decay of bacteria (see earlier studies [23,24] fo ...
... compare them directly with living organisms [18]. Although patterns of decay in animal embryos are now being studied [17,19–22], nothing is known about decay processes or preservation potential in giant sulphur-oxidizing bacteria. Previous studies on decay of bacteria (see earlier studies [23,24] fo ...
FST 305 GENERAL MICROBIOLOGY By Prof. Olusola Oyewole
... Selective medium and differential medium ...
... Selective medium and differential medium ...
Bacteria and Archaea
... divide every 20 minutes under ideal lab conditions, one reason it is used as a model organism in research. However, when growing in a human intestine, one of its natural environments, E. coli cells divide only once every 12–24 hours. But whether cell division occurs every 20 minutes or every few day ...
... divide every 20 minutes under ideal lab conditions, one reason it is used as a model organism in research. However, when growing in a human intestine, one of its natural environments, E. coli cells divide only once every 12–24 hours. But whether cell division occurs every 20 minutes or every few day ...
Direct interaction of FtsZ and MreB is required for septum synthesis
... proteins yield different subcellular patterns (Swulius and Jensen, 2012). Thus, the localisation pattern of MreB relative to FtsZ has not been firmly established in E. coli. Here, we report a novel role of MreB in bacterial cell division using E. coli as the model organism. Microscopic observations v ...
... proteins yield different subcellular patterns (Swulius and Jensen, 2012). Thus, the localisation pattern of MreB relative to FtsZ has not been firmly established in E. coli. Here, we report a novel role of MreB in bacterial cell division using E. coli as the model organism. Microscopic observations v ...
Isolation and Characterization of Conditional-Lethal Mutations in the TUB1 alpha-Tubulin Gene of the Yeast Saccharomyces cerevisiae .
... mutagenized plasmid pRB539, eight frommisincorporation mutagenesis andone from hydroxylamine mutagenesis, were introduced into yeast strain DBY2384. Before plating, the cells were incubated in SD minimal medium containing 45 pg/ml leucine and 30 pg/ml lysine for 30 min. Aliquots of 0.33 ml of cells ...
... mutagenized plasmid pRB539, eight frommisincorporation mutagenesis andone from hydroxylamine mutagenesis, were introduced into yeast strain DBY2384. Before plating, the cells were incubated in SD minimal medium containing 45 pg/ml leucine and 30 pg/ml lysine for 30 min. Aliquots of 0.33 ml of cells ...
Cell culture
Cell culture is the process by which cells are grown under controlled conditions, generally outside of their natural environment. In practice, the term ""cell culture"" now refers to the culturing of cells derived from multicellular eukaryotes, especially animal cells, in contrast with other types of culture that also grow cells, such as plant tissue culture, fungal culture, and microbiological culture (of microbes). The historical development and methods of cell culture are closely interrelated to those of tissue culture and organ culture. Viral culture is also related, with cells as hosts for the viruses. The laboratory technique of maintaining live cell lines (a population of cells descended from a single cell and containing the same genetic makeup) separated from their original tissue source became more robust in the middle 20th century.