1 - AState.edu
... • Proteins within a vesicle are usually modified by enzymes during their transit from the receiving to the shipping side of the Golgi apparatus. • The shipping side of a Golgi stack is a depot from which finished products can be carried in transport vesicles to other organelles or to the plasma memb ...
... • Proteins within a vesicle are usually modified by enzymes during their transit from the receiving to the shipping side of the Golgi apparatus. • The shipping side of a Golgi stack is a depot from which finished products can be carried in transport vesicles to other organelles or to the plasma memb ...
New Insights on Plant Cell Elongation: A Role for Acetylcholine
... stimulus is integrated and highly complex [1,2]. Recently, it has been proposed that auxin transport could involve secretion via an endocytic vesicle recycling process, at least at the root apex [3–8]. This vesicular secretion closely resembles the synaptic communication in animals [9–12] and, at th ...
... stimulus is integrated and highly complex [1,2]. Recently, it has been proposed that auxin transport could involve secretion via an endocytic vesicle recycling process, at least at the root apex [3–8]. This vesicular secretion closely resembles the synaptic communication in animals [9–12] and, at th ...
Tissues - Union County College
... epidermis – Outermost layers of epidermis are made up of dead, dried out epithelial cells which contain keratin (a fibrous protein, also a component of fingernails and hair) • When cells are dead and water has evaporated, keratin forms a tough barrier which microorganisms generally have a tough time ...
... epidermis – Outermost layers of epidermis are made up of dead, dried out epithelial cells which contain keratin (a fibrous protein, also a component of fingernails and hair) • When cells are dead and water has evaporated, keratin forms a tough barrier which microorganisms generally have a tough time ...
Lonza Group Ltd
... completed the acquisition of the preclinical cell and molecular biology assets of Simbiosys Biowares India Pvt. Ltd as of 13 October 2009. Simbiosys Biowares is a premier biology outsourcing company with core competencies in cell and molecular biology, and in clinical services. The company’s cell an ...
... completed the acquisition of the preclinical cell and molecular biology assets of Simbiosys Biowares India Pvt. Ltd as of 13 October 2009. Simbiosys Biowares is a premier biology outsourcing company with core competencies in cell and molecular biology, and in clinical services. The company’s cell an ...
Localization of Green Fluorescent Protein Fusions
... tobacco (Nicotiana tabacum) cells demonstrated that BP-80 and its homologs were predominantly concentrated on the lytic PVCs at steady state (Li et al., 2002). These results indicate that VSRs can be used as markers to define PVCs and that VSR proteins recycle back to Golgi briefly for cargo selecti ...
... tobacco (Nicotiana tabacum) cells demonstrated that BP-80 and its homologs were predominantly concentrated on the lytic PVCs at steady state (Li et al., 2002). These results indicate that VSRs can be used as markers to define PVCs and that VSR proteins recycle back to Golgi briefly for cargo selecti ...
LvNotch specifies secondary mesenchyme - Development
... all constructs except for LvNfull, which was injected at the highest possible concentration of 1.0-3.0 pg/zygote (see Methods). Protein immunoblot studies were carried out using protein extracts collected from 30 mRNA-injected blastulastage embryos (8-12 hours of development) for each construct. The ...
... all constructs except for LvNfull, which was injected at the highest possible concentration of 1.0-3.0 pg/zygote (see Methods). Protein immunoblot studies were carried out using protein extracts collected from 30 mRNA-injected blastulastage embryos (8-12 hours of development) for each construct. The ...
Leaf epidermal studies of three species of Acalypha Linn.
... headache and colds in Nigeria, the cold extracts of the leaves is used to bath babies with skin infection (Adesina et al., 2000). The leaf poultice is deam good for headache, swellings and cold in trinidad. Its leaf extract is active against Gram +ve bacteria, the extracts of seed have Immunomodulat ...
... headache and colds in Nigeria, the cold extracts of the leaves is used to bath babies with skin infection (Adesina et al., 2000). The leaf poultice is deam good for headache, swellings and cold in trinidad. Its leaf extract is active against Gram +ve bacteria, the extracts of seed have Immunomodulat ...
Klebsiella pneumoniae survives within macrophages by
... Copyright for the publications made accessible via the Queen's University Belfast Research Portal is retained by the author(s) and / or other copyright owners and it is a condition of accessing these publications that users recognise and abide by the legal requirements associated with these rights. ...
... Copyright for the publications made accessible via the Queen's University Belfast Research Portal is retained by the author(s) and / or other copyright owners and it is a condition of accessing these publications that users recognise and abide by the legal requirements associated with these rights. ...
Phison Le - American Academy of Optometry
... Benign limbal lesions do not stain with NaFl nor Rose-Bengal. Malignant lesions such as CIN appear gelatinous, with an irregular surface and leukoplakic or gray in appearance. Histological analysis remains gold standard for diagnosis of limbal lesions. Hairpin feeder vessels increases suspicion for ...
... Benign limbal lesions do not stain with NaFl nor Rose-Bengal. Malignant lesions such as CIN appear gelatinous, with an irregular surface and leukoplakic or gray in appearance. Histological analysis remains gold standard for diagnosis of limbal lesions. Hairpin feeder vessels increases suspicion for ...
Isoflavone and Pterocarpan Malonylglucosides and ß -l,3
... sults from other publications [38-40], As marker for the vacuolar compartment several hydrolytic enzymes were determined. a-Mannosidase, acid phosphatase and Mg 2+/K +-ATPase are known to be localized in the vacuoles [28, 40-42], The chick pea vacuoles also contained high levels of a-mannosidase, a ...
... sults from other publications [38-40], As marker for the vacuolar compartment several hydrolytic enzymes were determined. a-Mannosidase, acid phosphatase and Mg 2+/K +-ATPase are known to be localized in the vacuoles [28, 40-42], The chick pea vacuoles also contained high levels of a-mannosidase, a ...
Chlorophyll is not the primary photoreceptor for the
... Discrimination among these possibilities can be made using leaf cells completely lacking chlorophyll. Variegated young leaves of Coleus ´ hybridus grow at modest rates in the light. Leaf strips can be obtained with all mesophyll cells either containing (green) or completely lacking (white) chlorophy ...
... Discrimination among these possibilities can be made using leaf cells completely lacking chlorophyll. Variegated young leaves of Coleus ´ hybridus grow at modest rates in the light. Leaf strips can be obtained with all mesophyll cells either containing (green) or completely lacking (white) chlorophy ...
Embryonic stem cell differentiation: emergence of a new era in
... have uncovered a role for BMP4 in ES cell growth and demonstrated that in the presence of LIF, it can replace the requirement for serum. With these new developments, it is now possible to grow ES cells with defined factors in the absence of serum or feeder cells. Molecular analyses have revealed tha ...
... have uncovered a role for BMP4 in ES cell growth and demonstrated that in the presence of LIF, it can replace the requirement for serum. With these new developments, it is now possible to grow ES cells with defined factors in the absence of serum or feeder cells. Molecular analyses have revealed tha ...
biology and medicine Embryonic stem cell differentiation
... have uncovered a role for BMP4 in ES cell growth and demonstrated that in the presence of LIF, it can replace the requirement for serum. With these new developments, it is now possible to grow ES cells with defined factors in the absence of serum or feeder cells. Molecular analyses have revealed tha ...
... have uncovered a role for BMP4 in ES cell growth and demonstrated that in the presence of LIF, it can replace the requirement for serum. With these new developments, it is now possible to grow ES cells with defined factors in the absence of serum or feeder cells. Molecular analyses have revealed tha ...
Viral Antigens Recombinant Proteins
... • Packaged in vials per end-user’s titer and testing requirements • Contract manufacturing turn-around-time is 6-8 weeks • Tested for endotoxin and bioburden • Other BSL2 viruses and purified viral antigens are available In addition to live viruses, MLS expertise includes the manufacture of viral va ...
... • Packaged in vials per end-user’s titer and testing requirements • Contract manufacturing turn-around-time is 6-8 weeks • Tested for endotoxin and bioburden • Other BSL2 viruses and purified viral antigens are available In addition to live viruses, MLS expertise includes the manufacture of viral va ...
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... vividly exemplified by the differentiation of the neural crest, as well as by the arrangement of repeated embryonic elements that result in iterative anatomical patterns. Amongst these patterns, the peripheral nervous system and tissues derived from the somites (or that are patterned in association ...
... vividly exemplified by the differentiation of the neural crest, as well as by the arrangement of repeated embryonic elements that result in iterative anatomical patterns. Amongst these patterns, the peripheral nervous system and tissues derived from the somites (or that are patterned in association ...
Whole-cell biocatalysts by design - Microbial Cell Factories
... is especially preferred to catalysis by the isolated enzyme when the enzyme is unstable in vitro or requires expensive co-substrates or cofactors [29, 30]. In these cases, enzymes should be screened for high activity, efficient heterologous expression, and a method of blocking the catabolism of the ...
... is especially preferred to catalysis by the isolated enzyme when the enzyme is unstable in vitro or requires expensive co-substrates or cofactors [29, 30]. In these cases, enzymes should be screened for high activity, efficient heterologous expression, and a method of blocking the catabolism of the ...
Metabolic allometric scaling model. Combining cellular
... contributing to the value of allometric exponent, considering different biomolecular mechanisms participating in ATP turnover as the major contributors, added with "layering of function at various levels of organization", like different scale levels. Allometric exponents found separately for each co ...
... contributing to the value of allometric exponent, considering different biomolecular mechanisms participating in ATP turnover as the major contributors, added with "layering of function at various levels of organization", like different scale levels. Allometric exponents found separately for each co ...
3D Plus transfection reagent
... Optimal conditions vary depending on the nucleic acid, cell types, hydrogel types, and cell culture conditions. Therefore, the amounts and ratio of the individual components (DNA and 3D-PLUS Transfect ) may have to be adjusted to achieve best results. Accordingly, we suggest you to optimize the vari ...
... Optimal conditions vary depending on the nucleic acid, cell types, hydrogel types, and cell culture conditions. Therefore, the amounts and ratio of the individual components (DNA and 3D-PLUS Transfect ) may have to be adjusted to achieve best results. Accordingly, we suggest you to optimize the vari ...
Gastrulation: Making and Shaping Germ Layers
... (AP), dorsoventral (DV), and left-right (LR) embryonic axes. Other aspects of embryogenesis are specification of the germ layers, endoderm, mesoderm, and ectoderm, as well as their subsequent patterning and diversification of cell fates along the embryonic axes. These processes occur very early during ...
... (AP), dorsoventral (DV), and left-right (LR) embryonic axes. Other aspects of embryogenesis are specification of the germ layers, endoderm, mesoderm, and ectoderm, as well as their subsequent patterning and diversification of cell fates along the embryonic axes. These processes occur very early during ...
IFN and IL12 synergize to convert in vivo generated Th17 into Th1
... fivefold less IL-12Rb2 transcripts than in vitro-generated Th17 cells (Fig. 1B). Allowing the cells to rest did not significantly increase IL-12Rb2 expression of ex vivo isolated Th17 cells (data not shown), suggesting that Il12rb2 chain expression of Th17 cells is downregulated constitutively in vi ...
... fivefold less IL-12Rb2 transcripts than in vitro-generated Th17 cells (Fig. 1B). Allowing the cells to rest did not significantly increase IL-12Rb2 expression of ex vivo isolated Th17 cells (data not shown), suggesting that Il12rb2 chain expression of Th17 cells is downregulated constitutively in vi ...
Imaging the fate of histone Cse4 reveals de novo replacement in S
... of centromere–kinetochore interactions. Despite this simplicity, the architecture of Cse4 nucleosomes has become the subject of much debate. Cse4 nucleosomes have been reported to differ from the canonical nucleosome not only by the replacement of both molecules of histone H3 by the Cse4 variant, bu ...
... of centromere–kinetochore interactions. Despite this simplicity, the architecture of Cse4 nucleosomes has become the subject of much debate. Cse4 nucleosomes have been reported to differ from the canonical nucleosome not only by the replacement of both molecules of histone H3 by the Cse4 variant, bu ...
cilia regeneration in tetrahymena and its inhibition
... The cilia of Tetrahymena were amputated by the use of a procedure in which the cells remained viable and regenerated cilia. Deciliated cells were nonmotile, and cilia regeneration was assessed by scoring the percentage of motile cells at intervals following deciliation. After a 30-min lag, the decil ...
... The cilia of Tetrahymena were amputated by the use of a procedure in which the cells remained viable and regenerated cilia. Deciliated cells were nonmotile, and cilia regeneration was assessed by scoring the percentage of motile cells at intervals following deciliation. After a 30-min lag, the decil ...
Cell culture
Cell culture is the process by which cells are grown under controlled conditions, generally outside of their natural environment. In practice, the term ""cell culture"" now refers to the culturing of cells derived from multicellular eukaryotes, especially animal cells, in contrast with other types of culture that also grow cells, such as plant tissue culture, fungal culture, and microbiological culture (of microbes). The historical development and methods of cell culture are closely interrelated to those of tissue culture and organ culture. Viral culture is also related, with cells as hosts for the viruses. The laboratory technique of maintaining live cell lines (a population of cells descended from a single cell and containing the same genetic makeup) separated from their original tissue source became more robust in the middle 20th century.