Chapter 4 Lecture
... epithelial cells. 8. Using a light microscope, you examine a tissue and see a simple squamous epithelium on the outer surface. Can this be a sample of skin surface? 9. Name the two primary types of glandular epithelia. ...
... epithelial cells. 8. Using a light microscope, you examine a tissue and see a simple squamous epithelium on the outer surface. Can this be a sample of skin surface? 9. Name the two primary types of glandular epithelia. ...
Worksheet
... After that, the mass of water in beaker 3 is measured to find out the amount of water lost due to evaporation (initial volume is 50 g). Meanwhile, the mass of water in beaker 2 is also measured and the change in mass of water is compared with that in beaker 3. Meanwhile, the mass of potato in beaker ...
... After that, the mass of water in beaker 3 is measured to find out the amount of water lost due to evaporation (initial volume is 50 g). Meanwhile, the mass of water in beaker 2 is also measured and the change in mass of water is compared with that in beaker 3. Meanwhile, the mass of potato in beaker ...
Microenvironmental reprogramming by three
... dermal papillae were microdissected from adult human hair follicles and placed between the epidermis and dermis of dispase-separated foreskin tissue. On two occasions, recombined tissue, containing papillae from different male donors, was grafted onto the back of a SCID mouse for 6 wk (nine papillae ...
... dermal papillae were microdissected from adult human hair follicles and placed between the epidermis and dermis of dispase-separated foreskin tissue. On two occasions, recombined tissue, containing papillae from different male donors, was grafted onto the back of a SCID mouse for 6 wk (nine papillae ...
Natural sequence variants of yeast environmental sensors confer
... on populations of BY cells (strain GY51, pink) and RM cells (strain GY601, blue). Fluorescent values were corrected for cell size as indicated in the Materials and methods. Note that strain GY601 did not carry the original ura3D0 mutation previously associated with elevated noise, which implies that ...
... on populations of BY cells (strain GY51, pink) and RM cells (strain GY601, blue). Fluorescent values were corrected for cell size as indicated in the Materials and methods. Note that strain GY601 did not carry the original ura3D0 mutation previously associated with elevated noise, which implies that ...
Dual function for cloche in hematopoiesis - Development
... chicken aorta that express an endothelial-specific marker could subsequently differentiate into cells expressing a blood-specific surface antigen. In vitro studies have also shown that certain cells can give rise to both endothelium and blood (Choi et al., 1998; Nishikawa et al., 1998). Taken togeth ...
... chicken aorta that express an endothelial-specific marker could subsequently differentiate into cells expressing a blood-specific surface antigen. In vitro studies have also shown that certain cells can give rise to both endothelium and blood (Choi et al., 1998; Nishikawa et al., 1998). Taken togeth ...
Protruding Vulva Mutants Identify Novel Loci and Wnt
... 36 had high or moderately penetrant Pvl or Egl (egg-layingdefective) phenotypes and were characterized further (for most Pvl mutants the Egl phenotype is more penetrant than the Pvl phenotype). At this stage, 175 mutant strains were wild type, but went on to be Pvl; therefore many of these mutants m ...
... 36 had high or moderately penetrant Pvl or Egl (egg-layingdefective) phenotypes and were characterized further (for most Pvl mutants the Egl phenotype is more penetrant than the Pvl phenotype). At this stage, 175 mutant strains were wild type, but went on to be Pvl; therefore many of these mutants m ...
The relationship between emerging neural crest cells
... directly under the presumptive epidermis (Fig. IE). At more posterior levels of the embryo, however, staining in the dorsal basement membrane of the neural tube is very weak or absent (Fig. 1C,D). In some cases this is mirrored by weak staining of the adjacent epidermal basement membrane. This can b ...
... directly under the presumptive epidermis (Fig. IE). At more posterior levels of the embryo, however, staining in the dorsal basement membrane of the neural tube is very weak or absent (Fig. 1C,D). In some cases this is mirrored by weak staining of the adjacent epidermal basement membrane. This can b ...
1 - UvA-DARE - University of Amsterdam
... Condorelli paper. Since then a consensus has been reached and the protein is now called GFAPδ (Condorelli et al., 1999; Middeldorp and Hol, 2011; Nielsen et al., 2002; Thomsen et al., 2013). Blechingberg and colleagues studied alternative splicing with a GFAP minigene construct and showed that the e ...
... Condorelli paper. Since then a consensus has been reached and the protein is now called GFAPδ (Condorelli et al., 1999; Middeldorp and Hol, 2011; Nielsen et al., 2002; Thomsen et al., 2013). Blechingberg and colleagues studied alternative splicing with a GFAP minigene construct and showed that the e ...
Small Signaling Peptides in Arabidopsis Development
... completes activation. Cys-rich peptides often do not require proteolytic processing from a precursor, though there are exceptions. Activation of Cysrich peptides is completed upon formation of disulphide bonds between the conserved Cys residues of the peptide, thus bringing the peptide to an active ...
... completes activation. Cys-rich peptides often do not require proteolytic processing from a precursor, though there are exceptions. Activation of Cysrich peptides is completed upon formation of disulphide bonds between the conserved Cys residues of the peptide, thus bringing the peptide to an active ...
Summary of the Fiber Composition of Peripheral Nerves
... 4. Autonomic ganglia- source of POSTGANGLIONIC EFFERENT fibers to smooth muscle, cardiac muscle, and glands. Therefore, there are basically only four different types of nerve fibers in the peripheral nervous system, one afferent and three efferent. Although some of these four basic types can be subd ...
... 4. Autonomic ganglia- source of POSTGANGLIONIC EFFERENT fibers to smooth muscle, cardiac muscle, and glands. Therefore, there are basically only four different types of nerve fibers in the peripheral nervous system, one afferent and three efferent. Although some of these four basic types can be subd ...
Functional Microsatellite Polymorphism Associated with Divergent
... 3.5kbV1aR-luc construct (fig. 2). Because we were interested in the relationships among constructs within a cell line, we normalized the luciferase values for each construct to the luciferase values of the 3.5kbV1aR-luc for each cell line with each independent experiment. After two to three independ ...
... 3.5kbV1aR-luc construct (fig. 2). Because we were interested in the relationships among constructs within a cell line, we normalized the luciferase values for each construct to the luciferase values of the 3.5kbV1aR-luc for each cell line with each independent experiment. After two to three independ ...
The posterior section of the chick`s area pellucida
... (PM), which is part of the peripheral belt of the area pelucida (AP), and that they move into the hypoblast via Koller’s sickle (KS). Stern and Ireland (1981) and Stern (1990) claimed that the posterior cellular contribution was derived from the germ wall margin (GWM), or as they called it “the deep ...
... (PM), which is part of the peripheral belt of the area pelucida (AP), and that they move into the hypoblast via Koller’s sickle (KS). Stern and Ireland (1981) and Stern (1990) claimed that the posterior cellular contribution was derived from the germ wall margin (GWM), or as they called it “the deep ...
Urinalysis
... many of these RBC's, compared to two relatively normal RBC's at the center left of the right panel. These abnormal RBC's are dysmorphic RBC's. ...
... many of these RBC's, compared to two relatively normal RBC's at the center left of the right panel. These abnormal RBC's are dysmorphic RBC's. ...
- Columbia University Medical Center
... Figure 2. Expression and Subcellular Localization of SHH and Its Cleavage Products in Mammalian Cells (A) Detection of SHH amino-terminal cleavage product in stage 10 chick notochord. An - 2 0 kDa immunoractive band is detected in extracts of notochord but not dorsal neural tube tissue. Sizes (in ki ...
... Figure 2. Expression and Subcellular Localization of SHH and Its Cleavage Products in Mammalian Cells (A) Detection of SHH amino-terminal cleavage product in stage 10 chick notochord. An - 2 0 kDa immunoractive band is detected in extracts of notochord but not dorsal neural tube tissue. Sizes (in ki ...
Adherens Junctions, Desmosomes and Tight
... the organism from its environment and serves as its first line of structural and functional defense against dehydration, chemical substances, physical insults and micro-organisms. The living cell layers of the epidermis are crucial in the formation and maintenance of the barrier on two different lev ...
... the organism from its environment and serves as its first line of structural and functional defense against dehydration, chemical substances, physical insults and micro-organisms. The living cell layers of the epidermis are crucial in the formation and maintenance of the barrier on two different lev ...
Experimental approaches to study plant cell walls during plant
... linked to plant-microbe associations. Cellular imaging can be particularly important when applying a quantitative methodology to imaging techniques. Many microscopic techniques are available, including light (Wilt et al., 2009), fluorescence (Lichtman and Conchello, 2005), and confocal microscopy (N ...
... linked to plant-microbe associations. Cellular imaging can be particularly important when applying a quantitative methodology to imaging techniques. Many microscopic techniques are available, including light (Wilt et al., 2009), fluorescence (Lichtman and Conchello, 2005), and confocal microscopy (N ...
American Journal of Potato Research
... Failure of the zero tolerance regulation to eradicate ring rot from North America can be attributed at least in part to the presence of symptomless, latent infections. The term "latent infection" was first applied by Dykstra (10) in a subtitle to describe an experiment in which potatoes, inoculated ...
... Failure of the zero tolerance regulation to eradicate ring rot from North America can be attributed at least in part to the presence of symptomless, latent infections. The term "latent infection" was first applied by Dykstra (10) in a subtitle to describe an experiment in which potatoes, inoculated ...
Stringent Response Changes Cell Membrane Permeability in
... changes may also affect uptake and exclusion of other compounds such as antibiotics. To determine whether or not resistance was developed as a result of stringency, wild type and rel-/mutants were subjected to a killing assay. The killing assay (Fig. 3) measured death of cultures after 24 hours incu ...
... changes may also affect uptake and exclusion of other compounds such as antibiotics. To determine whether or not resistance was developed as a result of stringency, wild type and rel-/mutants were subjected to a killing assay. The killing assay (Fig. 3) measured death of cultures after 24 hours incu ...
MAIT Cells Precursor Derivatives Activating Mouse Derived Riboflavin
... bacteria relates to the riboflavin biosynthesis pathway. Indeed, although the enzymes of the riboflavin synthesis pathway are grouped in a highly regulated operon in Gram-positive bacteria, these enzymes are dispersed in the genome of Gram-negative bacteria, and subtle differences in the nature or t ...
... bacteria relates to the riboflavin biosynthesis pathway. Indeed, although the enzymes of the riboflavin synthesis pathway are grouped in a highly regulated operon in Gram-positive bacteria, these enzymes are dispersed in the genome of Gram-negative bacteria, and subtle differences in the nature or t ...
In all vertebrate embryos examined so far, the first
... Copyright © 2007 American Society of Hematology ...
... Copyright © 2007 American Society of Hematology ...
When cells get stressed: an integrative view of cellular senescence
... As mentioned above, yet other work has demonstrated that normal cells that are exposed to various physiologic stresses rapidly enter into a state of senescence, doing so within a period as short as several days. Such stresses include DNA-damaging agents, oxidative stress, “oncogenic stress” (due to ...
... As mentioned above, yet other work has demonstrated that normal cells that are exposed to various physiologic stresses rapidly enter into a state of senescence, doing so within a period as short as several days. Such stresses include DNA-damaging agents, oxidative stress, “oncogenic stress” (due to ...
Glycan-independent Role of Calnexin in the Intracellular
... performed as described previously (28). Monolayers of cells were lysed for 20 min in TNE/TX-100 buffer (20 mM Tris-HCl, pH 8.0, 150 mM NaCl, 5 mM EDTA, and 1% TX-100). Lysates were scraped from the dish and brought to 40% sucrose using 80% sucrose in TNE (20 mM Tris-HCl, pH 8.0, 150 mM NaCl, and 5 m ...
... performed as described previously (28). Monolayers of cells were lysed for 20 min in TNE/TX-100 buffer (20 mM Tris-HCl, pH 8.0, 150 mM NaCl, 5 mM EDTA, and 1% TX-100). Lysates were scraped from the dish and brought to 40% sucrose using 80% sucrose in TNE (20 mM Tris-HCl, pH 8.0, 150 mM NaCl, and 5 m ...
Excretory system - Faculty Support Site
... pigment, thus they appear yellow. Amino acids are derived from proteins in foods. They are used by cells for synthesis of new body protein or other nitrogen-containing molecules. The amino acids not used for synthesis are oxidized to generate energy or are converted to fats or carbohydrates that can ...
... pigment, thus they appear yellow. Amino acids are derived from proteins in foods. They are used by cells for synthesis of new body protein or other nitrogen-containing molecules. The amino acids not used for synthesis are oxidized to generate energy or are converted to fats or carbohydrates that can ...
Expression of the papillomavirus E2 protein in HeLa cells leads to
... The papillomavirus E2 protein plays a central role in the viral life cycle as it regulates both transcription and replication of the viral genome. In this study, we showed that transient expression of bovine papillomavirus type 1 or human papillomavirus type 18 (HPV18) E2 proteins in HeLa cells acti ...
... The papillomavirus E2 protein plays a central role in the viral life cycle as it regulates both transcription and replication of the viral genome. In this study, we showed that transient expression of bovine papillomavirus type 1 or human papillomavirus type 18 (HPV18) E2 proteins in HeLa cells acti ...
Cell culture
Cell culture is the process by which cells are grown under controlled conditions, generally outside of their natural environment. In practice, the term ""cell culture"" now refers to the culturing of cells derived from multicellular eukaryotes, especially animal cells, in contrast with other types of culture that also grow cells, such as plant tissue culture, fungal culture, and microbiological culture (of microbes). The historical development and methods of cell culture are closely interrelated to those of tissue culture and organ culture. Viral culture is also related, with cells as hosts for the viruses. The laboratory technique of maintaining live cell lines (a population of cells descended from a single cell and containing the same genetic makeup) separated from their original tissue source became more robust in the middle 20th century.